data_SASDFS2_MAIN # _sas_result.id 1305 _sas_result.SASBDB_code SASDFS2 _sas_result.experimental_MW 29.920 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 45.4100000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 13.220 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 1 _sas_result.Guinier_point_max 6 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73A) polymer frame no. 280-284 ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 280-284)" _sas_scan.detc_id 15 _sas_scan.id 1305 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1266 _sas_scan.result_id 1305 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1266 _sas_sample.name "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 280-284)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1266 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 4.058020E+01 1.173273E+01 1305 2 5.968380E-03 3.566280E+01 8.631668E+00 1305 3 6.388220E-03 3.458440E+01 6.260050E+00 1305 4 6.808050E-03 3.266820E+01 4.425412E+00 1305 5 7.227880E-03 3.682180E+01 4.064416E+00 1305 6 7.647710E-03 3.095400E+01 3.285944E+00 1305 7 8.067540E-03 2.733190E+01 2.600936E+00 1305 8 8.487370E-03 2.694560E+01 2.497852E+00 1305 9 8.907200E-03 2.380612E+01 2.157508E+00 1305 10 9.327030E-03 2.488230E+01 2.128646E+00 1305 11 9.746860E-03 2.305334E+01 1.817950E+00 1305 12 1.016670E-02 2.273208E+01 1.759514E+00 1305 13 1.058650E-02 2.112572E+01 1.517202E+00 1305 14 1.100630E-02 1.998514E+01 1.480754E+00 1305 15 1.142620E-02 1.926954E+01 1.391216E+00 1305 16 1.184600E-02 1.789302E+01 1.326152E+00 1305 17 1.226580E-02 1.608684E+01 1.165010E+00 1305 18 1.268570E-02 1.647786E+01 1.160410E+00 1305 19 1.310550E-02 1.457192E+01 1.097250E+00 1305 20 1.352530E-02 1.533572E+01 1.048764E+00 1305 21 1.394520E-02 1.362472E+01 9.616930E-01 1305 22 1.436500E-02 1.308488E+01 8.998874E-01 1305 23 1.478480E-02 1.172134E+01 8.267596E-01 1305 24 1.520460E-02 1.134148E+01 8.312858E-01 1305 25 1.562450E-02 1.096836E+01 7.798824E-01 1305 26 1.604430E-02 1.113944E+01 7.608074E-01 1305 27 1.646410E-02 9.838060E+00 7.184470E-01 1305 28 1.688400E-02 1.002598E+01 6.895250E-01 1305 29 1.730380E-02 9.532160E+00 6.342274E-01 1305 30 1.772360E-02 8.765860E+00 6.278322E-01 1305 31 1.814350E-02 8.760580E+00 6.155948E-01 1305 32 1.856330E-02 7.993520E+00 5.868698E-01 1305 33 1.898310E-02 7.558180E+00 5.526444E-01 1305 34 1.940290E-02 7.366580E+00 5.682694E-01 1305 35 1.982280E-02 7.286420E+00 5.246244E-01 1305 36 2.024260E-02 6.857940E+00 5.149180E-01 1305 37 2.066240E-02 6.117800E+00 5.201586E-01 1305 38 2.108230E-02 5.950020E+00 4.911252E-01 1305 39 2.150210E-02 5.543480E+00 5.061318E-01 1305 40 2.192190E-02 5.887000E+00 4.809888E-01 1305 41 2.234180E-02 5.098140E+00 4.757828E-01 1305 42 2.276160E-02 5.206800E+00 4.676302E-01 1305 43 2.318140E-02 4.901660E+00 4.522172E-01 1305 44 2.360130E-02 4.515900E+00 4.407846E-01 1305 45 2.402110E-02 4.408740E+00 4.460562E-01 1305 46 2.444090E-02 4.278320E+00 4.216596E-01 1305 47 2.486070E-02 4.225220E+00 4.144528E-01 1305 48 2.528060E-02 3.955940E+00 4.154752E-01 1305 49 2.570040E-02 3.623020E+00 4.050536E-01 1305 50 2.612020E-02 3.480180E+00 4.089680E-01 1305 51 2.654010E-02 3.657600E+00 4.052260E-01 1305 52 2.695990E-02 3.599180E+00 3.897880E-01 1305 53 2.737970E-02 3.463120E+00 3.972598E-01 1305 54 2.779960E-02 3.260360E+00 3.734592E-01 1305 55 2.821940E-02 2.675920E+00 3.806794E-01 1305 56 2.863920E-02 3.064900E+00 3.845298E-01 1305 57 2.905900E-02 2.601540E+00 3.714834E-01 1305 58 2.947890E-02 2.824080E+00 3.902180E-01 1305 59 2.989870E-02 2.717640E+00 3.684186E-01 1305 60 3.031850E-02 2.522000E+00 3.703250E-01 1305 61 3.073840E-02 2.456720E+00 3.565660E-01 1305 62 3.115820E-02 2.717960E+00 3.625492E-01 1305 63 3.157800E-02 2.525380E+00 3.539246E-01 1305 64 3.199790E-02 2.328880E+00 3.616546E-01 1305 65 3.241770E-02 2.170740E+00 3.850680E-01 1305 66 3.283750E-02 2.248420E+00 3.488036E-01 1305 67 3.325730E-02 2.399700E+00 3.615428E-01 1305 68 3.367720E-02 2.194360E+00 3.632600E-01 1305 69 3.409700E-02 1.743160E+00 3.525892E-01 1305 70 3.451680E-02 2.237580E+00 3.609710E-01 1305 71 3.493670E-02 2.119020E+00 3.497036E-01 1305 72 3.535650E-02 2.035700E+00 3.461450E-01 1305 73 3.577630E-02 1.725260E+00 3.482366E-01 1305 74 3.619620E-02 1.855780E+00 3.465930E-01 1305 75 3.661600E-02 1.951240E+00 3.336514E-01 1305 76 3.703580E-02 2.080980E+00 3.283486E-01 1305 77 3.745560E-02 1.626880E+00 3.298280E-01 1305 78 3.787550E-02 1.488360E+00 3.183408E-01 1305 79 3.829530E-02 1.870300E+00 3.188182E-01 1305 80 3.871510E-02 1.646160E+00 3.107372E-01 1305 81 3.913500E-02 1.420280E+00 3.113842E-01 1305 82 3.955480E-02 1.444560E+00 3.133124E-01 1305 83 3.997460E-02 1.679600E+00 3.023306E-01 1305 84 4.039450E-02 1.739060E+00 3.132168E-01 1305 85 4.081430E-02 1.497300E+00 3.054654E-01 1305 86 4.123410E-02 1.363580E+00 2.926782E-01 1305 87 4.165390E-02 1.618000E+00 3.061748E-01 1305 88 4.207380E-02 1.376400E+00 2.929578E-01 1305 89 4.249360E-02 1.373840E+00 2.911334E-01 1305 90 4.291340E-02 1.313140E+00 2.893812E-01 1305 91 4.333330E-02 1.346080E+00 2.915500E-01 1305 92 4.375310E-02 1.312520E+00 2.809726E-01 1305 93 4.417290E-02 1.256460E+00 2.774228E-01 1305 94 4.459280E-02 1.129400E+00 2.693954E-01 1305 95 4.501260E-02 1.144960E+00 2.680976E-01 1305 96 4.543240E-02 9.152602E-01 2.660792E-01 1305 97 4.585220E-02 1.297840E+00 2.721272E-01 1305 98 4.627210E-02 1.180400E+00 2.673350E-01 1305 99 4.669190E-02 1.351720E+00 2.645492E-01 1305 100 4.711170E-02 1.281620E+00 2.729084E-01 1305 101 4.753160E-02 9.160798E-01 2.622860E-01 1305 102 4.795140E-02 1.016380E+00 2.603586E-01 1305 103 4.837120E-02 1.149960E+00 2.561198E-01 1305 104 4.879110E-02 1.029320E+00 2.578428E-01 1305 105 4.921090E-02 9.483200E-01 2.512468E-01 1305 106 4.963070E-02 8.795200E-01 2.491178E-01 1305 107 5.005050E-02 1.091980E+00 2.516746E-01 1305 108 5.047040E-02 8.578006E-01 2.543448E-01 1305 109 5.089020E-02 8.579400E-01 2.545784E-01 1305 110 5.131000E-02 1.014180E+00 2.463178E-01 1305 111 5.172990E-02 6.432196E-01 2.495148E-01 1305 112 5.214970E-02 7.385200E-01 2.471712E-01 1305 113 5.256950E-02 1.169340E+00 2.514854E-01 1305 114 5.298940E-02 8.627600E-01 2.416372E-01 1305 115 5.340920E-02 8.541194E-01 2.450344E-01 1305 116 5.382900E-02 7.398398E-01 2.447202E-01 1305 117 5.424890E-02 7.688998E-01 2.402546E-01 1305 118 5.466870E-02 6.972800E-01 2.324518E-01 1305 119 5.508850E-02 6.866800E-01 2.376564E-01 1305 120 5.550830E-02 8.149200E-01 2.362390E-01 1305 121 5.592820E-02 8.480600E-01 2.345626E-01 1305 122 5.634800E-02 7.248802E-01 2.322524E-01 1305 123 5.676780E-02 7.092800E-01 2.385832E-01 1305 124 5.718770E-02 5.584196E-01 2.278340E-01 1305 125 5.760750E-02 7.571400E-01 2.282652E-01 1305 126 5.802730E-02 9.403398E-01 2.307720E-01 1305 127 5.844720E-02 5.137406E-01 2.223768E-01 1305 128 5.886700E-02 7.768398E-01 2.309628E-01 1305 129 5.928680E-02 7.113198E-01 2.239488E-01 1305 130 5.970660E-02 4.469398E-01 2.271980E-01 1305 131 6.012650E-02 8.332798E-01 2.265834E-01 1305 132 6.054630E-02 7.297004E-01 2.248632E-01 1305 133 6.096610E-02 7.502000E-01 2.192104E-01 1305 134 6.138600E-02 6.191602E-01 2.187350E-01 1305 135 6.180580E-02 7.481200E-01 2.228028E-01 1305 136 6.222560E-02 6.137200E-01 2.220510E-01 1305 137 6.264550E-02 7.942996E-01 2.192118E-01 1305 138 6.306530E-02 6.224396E-01 2.184674E-01 1305 139 6.348510E-02 6.727798E-01 2.160718E-01 1305 140 6.390490E-02 5.500400E-01 2.195540E-01 1305 141 6.432480E-02 7.602400E-01 2.105960E-01 1305 142 6.474460E-02 6.313400E-01 2.132228E-01 1305 143 6.516440E-02 7.187600E-01 2.149172E-01 1305 144 6.558430E-02 5.287800E-01 2.121900E-01 1305 145 6.600410E-02 6.612398E-01 2.129390E-01 1305 146 6.642390E-02 5.162198E-01 2.057038E-01 1305 147 6.684380E-02 6.018600E-01 2.176494E-01 1305 148 6.726360E-02 5.680200E-01 2.117786E-01 1305 149 6.768340E-02 4.909202E-01 2.111982E-01 1305 150 6.810320E-02 7.068600E-01 2.072290E-01 1305 151 6.852310E-02 6.626000E-01 2.090870E-01 1305 152 6.894290E-02 6.642600E-01 2.106512E-01 1305 153 6.936270E-02 5.803404E-01 2.032150E-01 1305 154 6.978260E-02 5.152610E-01 2.064808E-01 1305 155 7.020240E-02 4.521402E-01 2.043654E-01 1305 156 7.062220E-02 4.924404E-01 2.017854E-01 1305 157 7.104210E-02 7.131400E-01 2.072186E-01 1305 158 7.146190E-02 5.987200E-01 2.001882E-01 1305 159 7.188170E-02 4.861998E-01 2.026814E-01 1305 160 7.230160E-02 4.880996E-01 1.968172E-01 1305 161 7.272140E-02 4.483990E-01 2.004070E-01 1305 162 7.314120E-02 4.108400E-01 2.004480E-01 1305 163 7.356100E-02 3.949400E-01 1.972448E-01 1305 164 7.398090E-02 4.945599E-01 2.032538E-01 1305 165 7.440070E-02 4.140002E-01 1.972564E-01 1305 166 7.482050E-02 4.216202E-01 1.983750E-01 1305 167 7.524040E-02 3.961804E-01 1.964398E-01 1305 168 7.566020E-02 3.660998E-01 1.954878E-01 1305 169 7.608000E-02 3.341400E-01 1.972692E-01 1305 170 7.649980E-02 5.553400E-01 1.912984E-01 1305 171 7.691970E-02 3.538600E-01 1.966202E-01 1305 172 7.733950E-02 3.571002E-01 1.905768E-01 1305 173 7.775930E-02 4.589200E-01 1.900118E-01 1305 174 7.817920E-02 4.016400E-01 1.942364E-01 1305 175 7.859900E-02 4.278000E-01 1.912464E-01 1305 176 7.901880E-02 2.889798E-01 1.888402E-01 1305 177 7.943870E-02 4.051198E-01 1.895880E-01 1305 178 7.985850E-02 5.286598E-01 1.959124E-01 1305 179 8.027830E-02 3.976204E-01 1.882048E-01 1305 180 8.069810E-02 5.020800E-01 1.913474E-01 1305 181 8.111800E-02 4.500006E-01 1.937746E-01 1305 182 8.153780E-02 4.491198E-01 1.927864E-01 1305 183 8.195760E-02 4.416600E-01 1.877936E-01 1305 184 8.237750E-02 3.736995E-01 1.892692E-01 1305 185 8.279730E-02 3.823792E-01 1.908260E-01 1305 186 8.321710E-02 3.183402E-01 1.891044E-01 1305 187 8.363700E-02 4.625190E-01 1.941730E-01 1305 188 8.405680E-02 4.410998E-01 1.958818E-01 1305 189 8.447660E-02 2.014600E-01 1.912948E-01 1305 190 8.489650E-02 4.495598E-01 2.009300E-01 1305 191 8.531630E-02 4.130000E-01 2.039538E-01 1305 192 8.573610E-02 4.047004E-01 2.015812E-01 1305 193 8.615590E-02 3.261201E-01 2.037568E-01 1305 194 8.657580E-02 3.505207E-01 2.029646E-01 1305 195 8.699560E-02 4.951404E-01 2.032556E-01 1305 196 8.741540E-02 4.377404E-01 2.065390E-01 1305 197 8.783530E-02 4.698002E-01 2.023450E-01 1305 198 8.825510E-02 3.735394E-01 2.066602E-01 1305 199 8.867490E-02 2.716402E-01 2.105116E-01 1305 200 8.909480E-02 3.403599E-01 2.037708E-01 1305 201 8.951460E-02 2.713197E-01 2.019432E-01 1305 202 8.993440E-02 3.081396E-01 2.059832E-01 1305 203 9.035420E-02 4.320606E-01 2.090858E-01 1305 204 9.077410E-02 3.713596E-01 2.066604E-01 1305 205 9.119390E-02 2.981805E-01 2.068906E-01 1305 206 9.161370E-02 2.710800E-01 2.053940E-01 1305 207 9.203360E-02 3.074999E-01 2.055834E-01 1305 208 9.245340E-02 4.623603E-01 2.096524E-01 1305 209 9.287320E-02 1.158607E-01 2.063588E-01 1305 210 9.329310E-02 2.327596E-01 2.085678E-01 1305 211 9.371290E-02 3.557798E-01 2.101956E-01 1305 212 9.413270E-02 2.893394E-01 2.080376E-01 1305 213 9.455250E-02 1.875397E-01 2.078930E-01 1305 214 9.497240E-02 6.216014E-02 2.048946E-01 1305 215 9.539220E-02 2.791600E-01 2.094730E-01 1305 216 9.581200E-02 4.007600E-01 1.996172E-01 1305 217 9.623190E-02 2.445999E-01 2.042154E-01 1305 218 9.665170E-02 4.202600E-01 2.023820E-01 1305 219 9.707150E-02 2.058799E-01 2.011072E-01 1305 220 9.749140E-02 2.184998E-01 1.989000E-01 1305 221 9.791120E-02 2.051603E-01 2.007220E-01 1305 222 9.833100E-02 3.462601E-01 2.030292E-01 1305 223 9.875080E-02 2.041202E-01 1.964188E-01 1305 224 9.917070E-02 9.146060E-02 1.954510E-01 1305 225 9.959050E-02 2.318000E-01 1.953304E-01 1305 226 1.000100E-01 2.554196E-01 1.917102E-01 1305 227 1.004300E-01 1.052200E-01 1.975446E-01 1305 228 1.008500E-01 7.997998E-02 1.908236E-01 1305 229 1.012700E-01 2.040804E-01 1.980778E-01 1305 230 1.016900E-01 1.741200E-01 1.937912E-01 1305 231 1.021090E-01 3.351603E-01 1.941332E-01 1305 232 1.025290E-01 2.831392E-01 1.952764E-01 1305 233 1.029490E-01 3.100594E-01 1.884072E-01 1305 234 1.033690E-01 3.094800E-01 1.968038E-01 1305 235 1.037890E-01 1.945395E-01 1.961664E-01 1305 236 1.042090E-01 2.219996E-01 1.946040E-01 1305 237 1.046280E-01 2.329202E-01 1.932226E-01 1305 238 1.050480E-01 1.163003E-01 1.911136E-01 1305 239 1.054680E-01 1.293600E-01 1.929228E-01 1305 240 1.058880E-01 2.878605E-01 1.898024E-01 1305 241 1.063080E-01 2.461606E-01 1.916622E-01 1305 242 1.067280E-01 3.070600E-01 1.904186E-01 1305 243 1.071470E-01 2.926202E-01 1.865262E-01 1305 244 1.075670E-01 6.870030E-02 1.886592E-01 1305 245 1.079870E-01 1.738795E-01 1.857670E-01 1305 246 1.084070E-01 1.627403E-01 1.870546E-01 1305 247 1.088270E-01 2.585000E-01 1.869042E-01 1305 248 1.092470E-01 2.534199E-01 1.905010E-01 1305 249 1.096660E-01 2.463994E-01 1.859164E-01 1305 250 1.100860E-01 1.859988E-02 1.832740E-01 1305 251 1.105060E-01 3.054400E-01 1.874136E-01 1305 252 1.109260E-01 3.509800E-01 1.857050E-01 1305 253 1.113460E-01 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