{ "code": "SASDCS9", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "Signaling ammonium across membranes through an ammonium sensor histidine kinase.", "publication": { "title": "Signaling ammonium across membranes through an ammonium sensor histidine kinase.", "author_list": "Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA", "journal": "Nat Commun", "doi": "10.1038/s41467-017-02637-3", "pmid": "29323112", "published_date": "2018 Jan 11" }, "status": "released", "submitted_date": "2017-11-29", "released_date": "2018-01-16" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDCS9.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDCS9.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCS9_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCS9_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCS9_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCS9_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDCS9.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 2M", "resolution": null }, "name": "PETRA III", "city": "DESY; Hamburg", "country": "Germany", "beamline_name": "EMBL P12", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "ammonium sensor/transducer", "short_name": "Ks-Amt5", "sequence": "MENIQININHLWVIMAACMVFLMQLGFTSYETGFSQSKNAISIALRNLVDTLISSLVFFS\r\nVGFGFMFGKSYMGLIGIDLFFANDLALHPNTLSYSFFFFQMVFASTAATILTGAIAERSG\r\nFIPNIAGTAFIVAIIYPIFGHWAWGNLFSPDQTGWLKELGFIDFAGATVVHSIGGWFAMA\r\nAAIMVGPRIDKYNPDGSSNRIGLHNVPLATLGTFFLWFGWFGFNGGSLLRVSVNIGLVIL\r\nNTNMAAASAGVSALIFIYATRKRIEAGSLFTAILAGLVAITASSNMVTPVSAVAIGLITG\r\nILAIIAEGFIEKTLKIDDPVSAIAVHGVGGVIGTLCVAIFAQKSYLLAENGSRMHQLGIQ\r\nALGVIVAFSWSFGLGMLFFLCLKKVKRLRVTPEEEKRGLNVAEYEDVASWLDFIRITRLQ\r\nDINTILEKRVQEKTADLQMANVALEKANRLKSEFLTTMSHELRTPLNAIIGFAEVLRDEI\r\nAGSLSKDQKEYVTDIHSSGHHLLDMINNILDLSKIETGKMHLQYEEFCIEDAINDTLTII\r\nNASANNKGISVHTNIQDNTPLLSADKTKFRQILYNLLSNAVKFTPENGKITINVFQKDNS\r\nLQFEIVDTGIGIKPEDKEKLFEAFHQADASLTREYEGTGLGLHLTKRLVELHGGKIWAES\r\nTFGKGSTFFFILPINPVNKLEHHHHHH", "organism": "Candidatus Kuenenia stuttgartiensis", "uniprot_code": "Q1Q357", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "trimer", "molecular_type": "protein", "uniprot_sequence": "MENIQININHLWVIMAACMVFLMQLGFTSYETGFSQSKNAISIALRNLVDTLISSLVFFS\nVGFGFMFGKSYMGLIGIDLFFANDLALHPNTLSYSFFFFQMVFASTAATILTGAIAERSG\nFIPNIAGTAFIVAIIYPIFGHWAWGNLFSPDQTGWLKELGFIDFAGATVVHSIGGWFAMA\nAAIMVGPRIDKYNPDGSSNRIGLHNVPLATLGTFFLWFGWFGFNGGSLLRVSVNIGLVIL\nNTNMAAASAGVSALIFIYATRKRIEAGSLFTAILAGLVAITASSNMVTPVSAVAIGLITG\nILAIIAEGFIEKTLKIDDPVSAIAVHGVGGVIGTLCVAIFAQKSYLLAENGSRMHQLGIQ\nALGVIVAFSWSFGLGMLFFLCLKKVKRLRVTPEEEKRGLNVAEYEDVASWLDFIRITRLQ\nDINTILEKRVQEKTADLQMANVALEKANRLKSEFLTTMSHELRTPLNAIIGFAEVLRDEI\nAGSLSKDQKEYVTDIHSSGHHLLDMINNILDLSKIETGKMHLQYEEFCIEDAINDTLTII\nNASANNKGISVHTNIQDNTPLLSADKTKFRQILYNLLSNAVKFTPENGKITINVFQKDNS\nLQFEIVDTGIGIKPEDKEKLFEAFHQADASLTREYEGTGLGLHLTKRLVELHGGKIWAES\nTFGKGSTFFFILPINPVNK", "mw": 75.514, "total_mw": 226.542, "number_molecules": 3, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, 0.09%(w/v) CYMAL-5", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 8.0, "deuteration": null }, "purity_method": null, "name": "Ammonium transporter Ks-Amt5 (Batch)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": 95, "absorbption": null, "deuteration": null, "mixture": false }, "contributor": [ { "affiliation": [ { "short_name": "EMBL-Hamburg", "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany", "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation", "webpage": "http://www.embl-hamburg.de/index.php" } ], "contributor_name": "Haydyn", "contributor_surname": "Mertens", "orcid": null } ], "concentration_method": "A280", "concentration_unit": null, "date": "2013-03-02", "storage_temperature": 20.0, "cell_temperature": 20.0, "exposure_time": 0.05, "number_of_frames": 20, "wavelength": 0.124, "sample_detector_distance": 2.7, "concentration_min": null, "concentration_max": 1.0, "sample_volume": null, "flow_rate": null, "s_min": 0.148, "s_max": 4.423, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": "Excluded volume (DAMMIN)", "pddf_software": "ATSAS GNOM", "pddf_software_version": null, "i0_calibration_standard": "BSA", "description": "Batch mode Ks-Amt5 sample in 0.09% (w/v) CYMAL-5 detergent. Pooled fractions from offline SEC.", "experiment_description": null, "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 236.0, "experimental_mw_error": 30.0, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 283.0, "porod_mw_error": 30.0, "pddf_i0": 14060.0, "pddf_i0_error": 100.0, "guinier_i0": 14318.0, "guinier_i0_error": 160.0, "pddf_rg": 4.8, "pddf_rg_error": 0.1, "guinier_rg": 4.9, "guinier_rg_error": 0.1, "pddf_dmax": 17.0, "pddf_dmax_error": 0.5, "porod_volume": 482.0, "porod_volume_error": 50.0, "estimated_volume": 544.0, "estimated_volume_error": 50.0, "guinier_point_first": 1, "guinier_point_last": 44, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDCS9_datloglog_img.png", "symmetry": null, "last_modified": "2023-06-21T07:56:39.492710+02:00", "bragg_peak": [] }