data_SASDFT2_MAIN # _sas_result.id 1306 _sas_result.SASBDB_code SASDFT2 _sas_result.experimental_MW 29.920 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 46.5700000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 15.270 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 1 _sas_result.Guinier_point_max 8 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73A) polymer frame no 285-289 ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 285-289)" _sas_scan.detc_id 15 _sas_scan.id 1306 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1267 _sas_scan.result_id 1306 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1267 _sas_sample.name "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 285-289)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1267 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 3.691060E+01 1.187569E+01 1306 2 5.968380E-03 3.690380E+01 8.438388E+00 1306 3 6.388220E-03 3.510180E+01 6.220536E+00 1306 4 6.808050E-03 3.129120E+01 4.408438E+00 1306 5 7.227880E-03 3.055020E+01 3.805992E+00 1306 6 7.647710E-03 3.090320E+01 3.206712E+00 1306 7 8.067540E-03 2.524810E+01 2.916598E+00 1306 8 8.487370E-03 2.759960E+01 2.431110E+00 1306 9 8.907200E-03 2.448500E+01 2.069120E+00 1306 10 9.327030E-03 2.448018E+01 2.043822E+00 1306 11 9.746860E-03 2.417304E+01 1.843968E+00 1306 12 1.016670E-02 2.204624E+01 1.786738E+00 1306 13 1.058650E-02 2.109386E+01 1.575044E+00 1306 14 1.100630E-02 2.090256E+01 1.470302E+00 1306 15 1.142620E-02 1.865708E+01 1.302464E+00 1306 16 1.184600E-02 1.793702E+01 1.236732E+00 1306 17 1.226580E-02 1.705240E+01 1.223432E+00 1306 18 1.268570E-02 1.751584E+01 1.122500E+00 1306 19 1.310550E-02 1.533392E+01 1.137408E+00 1306 20 1.352530E-02 1.484230E+01 1.060423E+00 1306 21 1.394520E-02 1.522326E+01 9.769680E-01 1306 22 1.436500E-02 1.494656E+01 9.879708E-01 1306 23 1.478480E-02 1.269460E+01 8.339098E-01 1306 24 1.520460E-02 1.271184E+01 8.534316E-01 1306 25 1.562450E-02 1.234476E+01 8.107664E-01 1306 26 1.604430E-02 1.122708E+01 7.811532E-01 1306 27 1.646410E-02 1.113198E+01 6.930066E-01 1306 28 1.688400E-02 1.044678E+01 6.998054E-01 1306 29 1.730380E-02 9.607620E+00 6.702520E-01 1306 30 1.772360E-02 9.377120E+00 6.495502E-01 1306 31 1.814350E-02 9.140180E+00 6.071652E-01 1306 32 1.856330E-02 8.517380E+00 6.009812E-01 1306 33 1.898310E-02 8.595840E+00 5.817690E-01 1306 34 1.940290E-02 8.278740E+00 5.864430E-01 1306 35 1.982280E-02 8.012020E+00 5.394866E-01 1306 36 2.024260E-02 7.184420E+00 5.604616E-01 1306 37 2.066240E-02 6.454860E+00 5.140476E-01 1306 38 2.108230E-02 6.717880E+00 5.173180E-01 1306 39 2.150210E-02 6.382940E+00 5.156482E-01 1306 40 2.192190E-02 6.329220E+00 4.901774E-01 1306 41 2.234180E-02 6.470740E+00 4.948354E-01 1306 42 2.276160E-02 5.862860E+00 4.766338E-01 1306 43 2.318140E-02 5.600400E+00 4.479126E-01 1306 44 2.360130E-02 5.535100E+00 4.467334E-01 1306 45 2.402110E-02 5.235820E+00 4.408718E-01 1306 46 2.444090E-02 5.237840E+00 4.373738E-01 1306 47 2.486070E-02 4.590200E+00 4.284304E-01 1306 48 2.528060E-02 4.206380E+00 4.246428E-01 1306 49 2.570040E-02 4.437900E+00 4.049798E-01 1306 50 2.612020E-02 4.170540E+00 4.122812E-01 1306 51 2.654010E-02 4.244820E+00 4.034278E-01 1306 52 2.695990E-02 3.941980E+00 3.874294E-01 1306 53 2.737970E-02 3.675980E+00 3.917152E-01 1306 54 2.779960E-02 3.617340E+00 3.883570E-01 1306 55 2.821940E-02 3.740280E+00 3.891862E-01 1306 56 2.863920E-02 3.027420E+00 3.832780E-01 1306 57 2.905900E-02 3.166560E+00 3.752552E-01 1306 58 2.947890E-02 3.116960E+00 3.868102E-01 1306 59 2.989870E-02 3.388000E+00 3.784130E-01 1306 60 3.031850E-02 3.028360E+00 3.713410E-01 1306 61 3.073840E-02 2.956340E+00 3.765390E-01 1306 62 3.115820E-02 3.083160E+00 3.770214E-01 1306 63 3.157800E-02 2.788240E+00 3.703478E-01 1306 64 3.199790E-02 2.682200E+00 3.643486E-01 1306 65 3.241770E-02 2.665880E+00 3.742292E-01 1306 66 3.283750E-02 2.540860E+00 3.651240E-01 1306 67 3.325730E-02 2.181060E+00 3.612404E-01 1306 68 3.367720E-02 2.508240E+00 3.610746E-01 1306 69 3.409700E-02 2.250960E+00 3.386362E-01 1306 70 3.451680E-02 2.074620E+00 3.640852E-01 1306 71 3.493670E-02 2.217840E+00 3.430242E-01 1306 72 3.535650E-02 2.513640E+00 3.552682E-01 1306 73 3.577630E-02 1.983400E+00 3.393088E-01 1306 74 3.619620E-02 2.202300E+00 3.510726E-01 1306 75 3.661600E-02 2.134480E+00 3.393112E-01 1306 76 3.703580E-02 2.187920E+00 3.343692E-01 1306 77 3.745560E-02 1.856300E+00 3.357826E-01 1306 78 3.787550E-02 1.891000E+00 3.216714E-01 1306 79 3.829530E-02 1.724480E+00 3.201436E-01 1306 80 3.871510E-02 1.873140E+00 3.168974E-01 1306 81 3.913500E-02 1.834240E+00 3.140046E-01 1306 82 3.955480E-02 2.002480E+00 3.186798E-01 1306 83 3.997460E-02 1.707380E+00 3.076836E-01 1306 84 4.039450E-02 1.722640E+00 3.045270E-01 1306 85 4.081430E-02 1.474080E+00 3.096864E-01 1306 86 4.123410E-02 1.715900E+00 2.978570E-01 1306 87 4.165390E-02 1.401600E+00 3.025478E-01 1306 88 4.207380E-02 1.527720E+00 2.875636E-01 1306 89 4.249360E-02 1.614140E+00 2.915588E-01 1306 90 4.291340E-02 1.224740E+00 2.940884E-01 1306 91 4.333330E-02 1.610700E+00 2.886526E-01 1306 92 4.375310E-02 1.474320E+00 2.788724E-01 1306 93 4.417290E-02 1.215480E+00 2.716476E-01 1306 94 4.459280E-02 1.387340E+00 2.682730E-01 1306 95 4.501260E-02 1.528300E+00 2.698152E-01 1306 96 4.543240E-02 1.269100E+00 2.685742E-01 1306 97 4.585220E-02 1.243920E+00 2.688852E-01 1306 98 4.627210E-02 1.164660E+00 2.655590E-01 1306 99 4.669190E-02 1.559520E+00 2.681146E-01 1306 100 4.711170E-02 1.297680E+00 2.657708E-01 1306 101 4.753160E-02 1.371740E+00 2.673840E-01 1306 102 4.795140E-02 1.120820E+00 2.580696E-01 1306 103 4.837120E-02 1.215520E+00 2.567562E-01 1306 104 4.879110E-02 1.182880E+00 2.581086E-01 1306 105 4.921090E-02 1.005060E+00 2.545844E-01 1306 106 4.963070E-02 1.107700E+00 2.614888E-01 1306 107 5.005050E-02 8.756000E-01 2.514162E-01 1306 108 5.047040E-02 9.638402E-01 2.473426E-01 1306 109 5.089020E-02 9.779398E-01 2.497308E-01 1306 110 5.131000E-02 1.008700E+00 2.476504E-01 1306 111 5.172990E-02 7.306600E-01 2.502586E-01 1306 112 5.214970E-02 1.095240E+00 2.404480E-01 1306 113 5.256950E-02 9.641000E-01 2.519528E-01 1306 114 5.298940E-02 8.866200E-01 2.442400E-01 1306 115 5.340920E-02 9.089598E-01 2.442978E-01 1306 116 5.382900E-02 9.595400E-01 2.553034E-01 1306 117 5.424890E-02 7.447798E-01 2.371124E-01 1306 118 5.466870E-02 9.117600E-01 2.458922E-01 1306 119 5.508850E-02 1.070780E+00 2.367056E-01 1306 120 5.550830E-02 9.193600E-01 2.401282E-01 1306 121 5.592820E-02 9.215800E-01 2.348742E-01 1306 122 5.634800E-02 7.746602E-01 2.336230E-01 1306 123 5.676780E-02 7.746200E-01 2.370554E-01 1306 124 5.718770E-02 9.247998E-01 2.288626E-01 1306 125 5.760750E-02 7.683800E-01 2.296636E-01 1306 126 5.802730E-02 7.253200E-01 2.279068E-01 1306 127 5.844720E-02 5.701200E-01 2.271206E-01 1306 128 5.886700E-02 8.185198E-01 2.245192E-01 1306 129 5.928680E-02 7.729794E-01 2.222196E-01 1306 130 5.970660E-02 6.938200E-01 2.280054E-01 1306 131 6.012650E-02 9.073196E-01 2.254210E-01 1306 132 6.054630E-02 6.423400E-01 2.257078E-01 1306 133 6.096610E-02 7.687000E-01 2.269282E-01 1306 134 6.138600E-02 7.022202E-01 2.207376E-01 1306 135 6.180580E-02 7.964600E-01 2.221422E-01 1306 136 6.222560E-02 8.156002E-01 2.197772E-01 1306 137 6.264550E-02 6.893196E-01 2.200954E-01 1306 138 6.306530E-02 8.079798E-01 2.182488E-01 1306 139 6.348510E-02 7.307996E-01 2.181948E-01 1306 140 6.390490E-02 5.829802E-01 2.170110E-01 1306 141 6.432480E-02 6.761396E-01 2.142898E-01 1306 142 6.474460E-02 6.923800E-01 2.149372E-01 1306 143 6.516440E-02 8.217200E-01 2.169380E-01 1306 144 6.558430E-02 5.465600E-01 2.130018E-01 1306 145 6.600410E-02 7.451000E-01 2.153554E-01 1306 146 6.642390E-02 5.355000E-01 2.097668E-01 1306 147 6.684380E-02 9.161800E-01 2.144138E-01 1306 148 6.726360E-02 6.818602E-01 2.081594E-01 1306 149 6.768340E-02 6.382200E-01 2.109918E-01 1306 150 6.810320E-02 5.701002E-01 2.088330E-01 1306 151 6.852310E-02 7.105206E-01 2.092230E-01 1306 152 6.894290E-02 5.222396E-01 2.120598E-01 1306 153 6.936270E-02 6.789804E-01 2.041496E-01 1306 154 6.978260E-02 6.602800E-01 2.039284E-01 1306 155 7.020240E-02 5.645602E-01 2.049606E-01 1306 156 7.062220E-02 4.694602E-01 2.028080E-01 1306 157 7.104210E-02 6.448200E-01 2.014750E-01 1306 158 7.146190E-02 5.338802E-01 2.027586E-01 1306 159 7.188170E-02 4.910998E-01 1.992866E-01 1306 160 7.230160E-02 6.703996E-01 2.020984E-01 1306 161 7.272140E-02 4.623998E-01 1.965430E-01 1306 162 7.314120E-02 6.050000E-01 2.029638E-01 1306 163 7.356100E-02 4.901200E-01 1.946636E-01 1306 164 7.398090E-02 4.159800E-01 1.981276E-01 1306 165 7.440070E-02 4.830002E-01 1.953482E-01 1306 166 7.482050E-02 5.291404E-01 1.975790E-01 1306 167 7.524040E-02 4.567604E-01 1.949140E-01 1306 168 7.566020E-02 4.137600E-01 1.896642E-01 1306 169 7.608000E-02 4.820600E-01 1.956260E-01 1306 170 7.649980E-02 5.626000E-01 1.951842E-01 1306 171 7.691970E-02 3.415402E-01 1.933312E-01 1306 172 7.733950E-02 3.514803E-01 1.913680E-01 1306 173 7.775930E-02 4.855800E-01 1.902006E-01 1306 174 7.817920E-02 4.902000E-01 1.919920E-01 1306 175 7.859900E-02 2.716004E-01 1.903942E-01 1306 176 7.901880E-02 4.364800E-01 1.949600E-01 1306 177 7.943870E-02 4.408996E-01 1.909148E-01 1306 178 7.985850E-02 4.850394E-01 1.907860E-01 1306 179 8.027830E-02 4.364402E-01 1.905912E-01 1306 180 8.069810E-02 4.354996E-01 1.923318E-01 1306 181 8.111800E-02 2.866203E-01 1.932606E-01 1306 182 8.153780E-02 5.300598E-01 1.921476E-01 1306 183 8.195760E-02 4.404200E-01 1.913784E-01 1306 184 8.237750E-02 2.670798E-01 1.908550E-01 1306 185 8.279730E-02 4.044598E-01 1.846368E-01 1306 186 8.321710E-02 3.595202E-01 1.926564E-01 1306 187 8.363700E-02 4.640796E-01 1.944714E-01 1306 188 8.405680E-02 3.040800E-01 1.976658E-01 1306 189 8.447660E-02 4.389400E-01 1.975406E-01 1306 190 8.489650E-02 3.371800E-01 1.993704E-01 1306 191 8.531630E-02 2.432198E-01 2.020236E-01 1306 192 8.573610E-02 3.332406E-01 2.011720E-01 1306 193 8.615590E-02 4.526800E-01 2.057250E-01 1306 194 8.657580E-02 5.374002E-01 2.036536E-01 1306 195 8.699560E-02 2.343202E-01 1.991068E-01 1306 196 8.741540E-02 4.997602E-01 2.038988E-01 1306 197 8.783530E-02 4.275200E-01 2.057760E-01 1306 198 8.825510E-02 3.414193E-01 2.058426E-01 1306 199 8.867490E-02 4.082407E-01 2.021934E-01 1306 200 8.909480E-02 3.351600E-01 2.085726E-01 1306 201 8.951460E-02 3.833200E-01 2.090484E-01 1306 202 8.993440E-02 3.441392E-01 2.032858E-01 1306 203 9.035420E-02 4.114400E-01 2.072872E-01 1306 204 9.077410E-02 2.502800E-01 2.037486E-01 1306 205 9.119390E-02 3.338001E-01 2.043178E-01 1306 206 9.161370E-02 2.579800E-01 2.095920E-01 1306 207 9.203360E-02 2.799600E-01 2.066754E-01 1306 208 9.245340E-02 2.268001E-01 2.075280E-01 1306 209 9.287320E-02 2.423601E-01 2.042408E-01 1306 210 9.329310E-02 3.274796E-01 2.069188E-01 1306 211 9.371290E-02 3.532600E-01 2.161102E-01 1306 212 9.413270E-02 2.792799E-01 2.074886E-01 1306 213 9.455250E-02 3.293798E-01 2.055092E-01 1306 214 9.497240E-02 2.966200E-01 2.029322E-01 1306 215 9.539220E-02 2.957600E-01 2.057344E-01 1306 216 9.581200E-02 4.088800E-01 2.031722E-01 1306 217 9.623190E-02 4.089800E-01 2.068396E-01 1306 218 9.665170E-02 1.335601E-01 1.984156E-01 1306 219 9.707150E-02 3.435597E-01 1.997798E-01 1306 220 9.749140E-02 8.043980E-02 1.993802E-01 1306 221 9.791120E-02 2.286403E-01 1.988760E-01 1306 222 9.833100E-02 1.824601E-01 2.005262E-01 1306 223 9.875080E-02 2.626002E-01 1.930156E-01 1306 224 9.917070E-02 2.258202E-01 1.973722E-01 1306 225 9.959050E-02 2.855400E-01 1.972208E-01 1306 226 1.000100E-01 2.835597E-01 1.945942E-01 1306 227 1.004300E-01 2.099000E-01 1.979346E-01 1306 228 1.008500E-01 3.054198E-01 1.950386E-01 1306 229 1.012700E-01 2.543202E-01 1.967998E-01 1306 230 1.016900E-01 3.965000E-01 1.921838E-01 1306 231 1.021090E-01 2.295203E-01 1.918280E-01 1306 232 1.025290E-01 1.332994E-01 1.936550E-01 1306 233 1.029490E-01 4.017398E-01 1.902412E-01 1306 234 1.033690E-01 2.861000E-01 1.933714E-01 1306 235 1.037890E-01 2.332396E-01 1.928624E-01 1306 236 1.042090E-01 2.923591E-01 1.931594E-01 1306 237 1.046280E-01 2.206805E-01 1.942338E-01 1306 238 1.050480E-01 3.840400E-01 1.914492E-01 1306 239 1.054680E-01 3.392200E-01 1.956542E-01 1306 240 1.058880E-01 1.769600E-01 1.910432E-01 1306 241 1.063080E-01 3.428002E-01 1.948100E-01 1306 242 1.067280E-01 2.362404E-01 1.894034E-01 1306 243 1.071470E-01 2.085803E-01 1.893796E-01 1306 244 1.075670E-01 2.854404E-01 1.933566E-01 1306 245 1.079870E-01 2.289394E-01 1.868524E-01 1306 246 1.084070E-01 2.432803E-01 1.883290E-01 1306 247 1.088270E-01 1.394597E-01 1.853300E-01 1306 248 1.092470E-01 2.420400E-01 1.883348E-01 1306 249 1.096660E-01 2.196592E-01 1.862144E-01 1306 250 1.100860E-01 2.503000E-01 1.855310E-01 1306 251 1.105060E-01 1.776198E-01 1.888010E-01 1306 252 1.109260E-01 2.672400E-01 1.838716E-01 1306 253 1.113460E-01 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