{ "code": "SASDET5", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "Structural studies of Old Yellow Enzyme of Leishmania braziliensis in solution.", "publication": { "title": "Structural studies of Old Yellow Enzyme of Leishmania braziliensis in solution.", "author_list": "Walbert Veloso-Silva LL, Dores-Silva PR, Bertolino Reis DE, Moreno Oliveira LF, Libardi SH, Borges JC", "journal": "Arch Biochem Biophys", "doi": "10.1016/j.abb.2018.11.009", "pmid": "30447208", "published_date": "2018 Nov 14" }, "status": "released", "submitted_date": "2018-10-05", "released_date": "2018-11-19" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDET5.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDET5.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDET5_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDET5_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDET5_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDET5_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDET5.sascif", "experiment": { "instrument": { "detector": { "type": "20Hz", "name": "Pilatus 300K", "resolution": null }, "name": "Brazilian Synchrotron Light Laboratory", "city": "Campinas", "country": "Brazil", "beamline_name": "SAXS1 Beamline", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Old Yellow Enzyme of Leishmania braziliensis", "short_name": "LbOYE", "sequence": ">LbOYE_deltaHis\r\nGSEFDYTMPTASNSIDALLKPLVIGQLSMPNRFVMAPLTRCRADDEHVPTPAMVRHYADR\r\nASMGLIITEATQIEKGYSTFCHEGGIYGKEQVDGWRKVTDAVHEKGGLIFCQIHNGGRAT\r\nVPSNVDEGVRIVAPSAVAITDHECPGAFARNGEKQPYPVPHEMTAEDIALYVKLYATAAC\r\nNAMAAGFDGVEVHGANGYLIDEFLKSSSNQRTDEYGGSIENRCRFLFEVLDAVIKAIGRE\r\nRVGLRISPLNSFNSQSDENPEALTRYICSQLNSRNISFLDVMRGDFFSQACGADKWAREA\r\nYEGVLFTGMGFEIEEAAETVASGAADAVVFGTKALANPDLVARAIAGAALNTPDPATFYS\r\nTSEAGYNDYPSMVSSCPSAAAPSCTKETP", "organism": "Leishmania braziliensis", "uniprot_code": null, "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": null, "mw": 41.859, "total_mw": 41.859, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "25 mM Tris-HCl 100 mM NaCl and 1 mM β-mercaptoethanol", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 8.0, "deuteration": null }, "purity_method": null, "name": "Old Yellow Enzyme of Leishmania braziliensis", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "São Carlos - SP - Brasil", "full_name": "Instituto de Química de São Carlos", "webpage": "" } ], "contributor_name": "Júlio", "contributor_surname": "Borges", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2015-05-26", "storage_temperature": 4.0, "cell_temperature": 25.0, "exposure_time": 360.0, "number_of_frames": 1, "wavelength": 0.148, "sample_detector_distance": 1.0, "concentration_min": null, "concentration_max": 3.0, "sample_volume": null, "flow_rate": null, "s_min": 0.234, "s_max": 3.181, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDET5_fit1_model1_img.png", "software": "DAMMIN", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDET5_fit1_model1.pdb", "model_mw": 43.075, "bead_radius": 2.4, "log": null, "symmetry": null, "comment": null, "user": 161 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/LbOYE_fit_img.png", "software": "DAMMIN", "chi_square_value": 6.159, "p_value": 2e-06, "fit_residual_plot": "LbOYE_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDET5_fit1.fir", "fit_log": null, "software_version": null, "description": "" } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "4.6", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of old yellow enzyme in 25 mM Tris-HCl 100 mM NaCl and 1 mM β-mercaptoethanol, pH 8 were collected on the SAXS1 beam line at the Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a 20Hz Pilatus 300K detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.148 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 25°C. One 360 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.", "tags": [], "intensity_unit": "1/cm", "experimental_mw": 40.0, "experimental_mw_error": null, "guinier_i0_mw": 48.6, "guinier_i0_mw_error": 4.0, "porod_mw": 40.0, "porod_mw_error": null, "pddf_i0": 0.3768, "pddf_i0_error": 0.037, "guinier_i0": 0.381, "guinier_i0_error": 0.0007, "pddf_rg": 2.69, "pddf_rg_error": 0.2, "guinier_rg": 2.71, "guinier_rg_error": null, "pddf_dmax": 9.5, "pddf_dmax_error": 1.0, "porod_volume": 73.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 16, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDET5_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }