data_SASDCT6_MAIN
#
_sas_result.id                            666
_sas_result.SASBDB_code                   SASDCT6
_sas_result.experimental_MW               105.000
_sas_result.experimental_MW_error         .
_sas_result.MW_standard                   .
_sas_result.MW_standard_error             .
_sas_result.MW_Porod                      .
_sas_result.MW_Porod_error                .
_sas_result.I0_from_PR                    0.0222000000
_sas_result.I0_from_PR_error              0.0001300000
_sas_result.I0_from_Guinier               0.0216220000
_sas_result.I0_from_Guinier_error         0.0001760000
_sas_result.Rg_from_PR                    4.937
_sas_result.Rg_from_PR_error              0.026
_sas_result.Rg_from_Guinier               4.812
_sas_result.Rg_from_Guinier_error         0.520
_sas_result.D_max                         14.00
_sas_result.Porod_volume                  .
_sas_result.Porod_volume_error            .
_sas_result.estimated_volume              .
_sas_result.estimated_volume_error        .
_sas_result.estimated_volume_method       .
_sas_result.Guinier_point_min             1
_sas_result.Guinier_point_max             12
_sas_result.type_of_curve                 single_conc
_sas_result.standard                      .
_sas_result.Dmax_error                    .
_sas_result.comments                      
;12N12 nucleosome measured in 60% sucrose and ADP-BeF3.  
Due to contrast matching between the solvent and histone proteins (due to sucrose), the measured signal is dominated by the DNA component. The model was generated using 3D-DART and custom scripts written in Pymol. The percentage-weighting of each model in the ensemble ('XXXperc') is noted in the model key below: 
SASDCT6_fit1_model1 = 12N12_ADP_01_100perc
;

#
_sas_scan.title                     "12N12 nucleosome in 60% sucrose with ADP-BeF3"
_sas_scan.detc_id                   39
_sas_scan.id                        666
_sas_scan.measurement_date          2015-10-24
_sas_scan.storage_temperature       25.0
_sas_scan.cell_temperature          25.0
_sas_scan.exposure_time             10.000
_sas_scan.number_of_frames          40
_sas_scan.unit                      1/A
_sas_scan.qmin                      0.0160
_sas_scan.qmax                      0.2600
_sas_scan.sample_id                 624
_sas_scan.result_id                 666
_sas_scan.beam_id                   60
#
_sas_beam.id                         60
_sas_beam.instrument_name            "Cornell High Energy Synchrotron Source (CHESS) G1"
_sas_beam.instrument_city            "Ithaca, NY"
_sas_beam.instrument_country         USA
_sas_beam.shape                      .
_sas_beam.type_of_source             "X-ray synchrotron"
_sas_beam.radiation_wavelength       0.110900
_sas_beam.dist_spec_to_detc          2.08000
#
_sas_detc.id              39
_sas_detc.name            "Pilatus 200K"
_sas_detc.type            Pilatus
_sas_detc.pixsize_x       172.000
#
_sas_sample.id                           624
_sas_sample.name                         "12N12 nucleosome in 60% sucrose with ADP-BeF3"
_sas_sample.ext_coefficient              .
_sas_sample.contrast                     .
_sas_sample.specific_vol                 .
_sas_sample.purity_method                .
_sas_sample.dry_vol                      .
_sas_sample.sample_MW                    .
_sas_sample.specimen_concentration       1.10
_sas_sample.concentration_method         .
loop_
_sas_sample.entity_id                    
769
770
771
772
773
#
_sas_buffer.id                  385
_sas_buffer.name                "10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose, ADP-BeF3 (0.5 mM ADP, 4 mM NaF, 0.6 mM BeCl2)"
_sas_buffer.concentration       .
_sas_buffer.unit                .
_sas_buffer.pK                  .
_sas_buffer.pH                  7.800
_sas_buffer.comment             "60% sucrose ADP-BeF3"
_sas_buffer.composition         .
_sas_buffer.sample_id           624
#
loop_
_entity.id                             
_entity.type                           
_entity.pdbx_description               
_entity.formula_weight                 
_entity.pdbx_number_of_molecules       
769 DNA     "169 bp DNA (145 bp Widom 601, flanked by 12bp DNA)" 52.468 1
770 protein "Histone H2A type 1"                                 13.966 1
771 protein "Histone H2B 1.1"                                    13.934 1
772 protein "Histone H3.2"                                       15.388 1
773 protein "Histone H4"                                         11.367 1
#
loop_
_entity_name_com.entity_id       
_entity_name_com.name            
769 DNA
770 H2A
771 H2B
772 H3 
773 H4 
#
loop_
_entity_poly.entity_id                      
_entity_poly.pdbx_seq_one_letter_code       
769 CTTTCAGCTGATATCGATGTATATATCTGACACGTGCCTGGAGACTAGGGAGTAATCCCCTTGGCGGTTAAAACGCGGGGGACAGCGCGTACGTGCGTTTAAGCGGTGCTAGAGCTGTCTACGACCAATTGAGCGGCCTCGGCACCGGGATCTTGATATCAGCTGAAAG
770 
;MSGRGKQGGKTRAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLT
AEILELAGNAARDNKKTRIIPRHLQLAVRNDEELNKLLGGVTIAQGGVLPNIQSVLLPKK
TESAKSAKSK
;

771 
;MPEPAKSAPAPKKGSKKAVTKTQKKDGKKRRKSRKESYAIYVYKVLKQVHPDTGISSKAM
SIMNSFVNDVFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVT
KYTSAK
;

772 
;MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTE
LLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTI
MPKDIQLARRIRGERA
;

773 
;MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK
VFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG
;

#
loop_
_entity_src_gen.entity_id                  
_entity_src_gen.gene_src_common_name       
769 .               
770 "Xenopus laevis"
771 "Xenopus laevis"
772 "Xenopus laevis"
773 "Xenopus laevis"
#
loop_
_struct_ref.id              
_struct_ref.entity_id       
_struct_ref.db_name         
_struct_ref.db_code         
_struct_ref.details         
1 769 UniProt .      http://www.uniprot.org/uniprot/P06897
2 770 UniProt P06897 http://www.uniprot.org/uniprot/P02281
3 771 UniProt P02281 http://www.uniprot.org/uniprot/P84233
4 772 UniProt P84233 http://www.uniprot.org/uniprot/P62799