{ "code": "SASDDT8", "status": "Published", "type_of_curve": "Merged", "angular_unit": "1/nm", "project": { "title": "Structures of insect Imp-L2 suggest an alternative strategy for regulating the bioavailability of insulin-like hormones.", "publication": { "title": "Structures of insect Imp-L2 suggest an alternative strategy for regulating the bioavailability of insulin-like hormones.", "author_list": "Roed NK, Viola CM, Kristensen O, Schluckebier G, Norrman M, Sajid W, Wade JD, Andersen AS, Kristensen C, Ganderton TR, Turkenburg JP, De Meyts P, Brzozowski AM", "journal": "Nat Commun", "doi": "10.1038/s41467-018-06192-3", "pmid": "30242155", "published_date": "2018 Sep 21" }, "status": "released", "submitted_date": "2018-06-21", "released_date": "2018-12-03" }, "pddf_data": null, "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDDT8.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDT8_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDT8_kratky_img.png", "pddf_plot": null, "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDT8_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDDT8.sascif", "experiment": { "instrument": { "detector": { "type": "Dectris", "name": "Pilatus 1M", "resolution": 172.0 }, "name": "ESRF", "city": "Grenoble", "country": "France", "beamline_name": "ID14-3", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": true, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Insulin-like peptide 5", "short_name": null, "sequence": "NSLRACGPALMDMLRVACPNGFNSMFAK\r\nGVVDSCCRKSCSFSTLRAYCDS", "organism": "Drosophila melanogaster", "uniprot_code": "E7BBS4", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MFRSVIPVLLFLIPLLLSAQAANSLRACGPALMDMLRVACPNGFNSMFAKRGTLGLFDYE\nDHLADLDSSESHHMNSLSSIRRDFRGVVDSCCRKSCSFSTLRAYCDS", "mw": 5.385, "total_mw": 5.385, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null }, { "long_name": "Neural/ectodermal development factor IMP-L2", "short_name": "Imp-L2 IBP", "sequence": "MEAKMNLHVCALALLLFGSIATVRGRAVDLVDDSNDVDNSIEAEEEKPRNRAFEADWLKF\r\nTKTPPTKLQQADGATIEIVCEMMGSQVPSIQWVVGHLPRSELDDLDSNQVAEEAPSAIVR\r\nVRSSHIIDHVLSEARTYTCVGRTGSKTIYASTVVHPPRSSRLTPEKTYPGAQKPRIIYTE\r\nKTHLDLMGSNIQLPCRVHARPRAEITWLNNENKEIVQGHRHRVLANGDLLISEIKWEDMG\r\nNYKCIARNVVGKDTADTFVYPVLNEED", "organism": "Drosophila melanogaster", "uniprot_code": "Q09024", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MEAKMNLHVCALALLLFGSIATVRGRAVDLVDDSNDVDNSIEAEEEKPRNRAFEADWLKF\nTKTPPTKLQQADGATIEIVCEMMGSQVPSIQWVVGHLPRSELDDLDSNQVAEEAPSAIVR\nVRSSHIIDHVLSEARTYTCVGRTGSKTIYASTVVHPPRSSRLTPEKTYPGAQKPRIIYTE\nKTHLDLMGSNIQLPCRVHARPRAEITWLNNENKEIVQGHRHRVLANGDLLISEIKWEDMG\nNYKCIARNVVGKDTADTFVYPVLNEED", "mw": 29.895, "total_mw": 29.895, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "phosphate buffered saline", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.4, "deuteration": null }, "purity_method": null, "name": "Neural/ectodermal development factor IMP-L2 in complex with insulin-like peptide 5 (DILP5)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Denmark", "full_name": "Novo Nordisk A/S", "webpage": "https://www.novonordisk.com/" } ], "contributor_name": "Mathias", "contributor_surname": "Norrman", "orcid": "" } ], "concentration_method": null, "concentration_unit": null, "date": "2011-11-20", "storage_temperature": 5.0, "cell_temperature": 25.0, "exposure_time": 10.0, "number_of_frames": 10, "wavelength": 0.093, "sample_detector_distance": 2.43, "concentration_min": 2.3, "concentration_max": 9.3, "sample_volume": null, "flow_rate": null, "s_min": 0.064, "s_max": 6.108, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": null, "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of neural/ectodermal development factor IMP-L2 in complex with insulin-like peptide 5 (DILP5) in phosphate buffered saline, pH 7.4 were collected on the ID14-3 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.093 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2.3 and 9.3 mg/ml were measured at 25°C. 10 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 30.0, "experimental_mw_error": null, "guinier_i0_mw": 30.0, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": null, "pddf_i0_error": null, "guinier_i0": 44.77, "guinier_i0_error": 0.03, "pddf_rg": null, "pddf_rg_error": null, "guinier_rg": 2.59, "guinier_rg_error": 0.04, "pddf_dmax": 9.0, "pddf_dmax_error": null, "porod_volume": 55.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 26, "guinier_point_last": 65, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDDT8_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }