data_SASDFU2_MAIN # _sas_result.id 1307 _sas_result.SASBDB_code SASDFU2 _sas_result.experimental_MW 29.920 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 41.4700000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 14.480 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 3 _sas_result.Guinier_point_max 9 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73A) polymer frame no. 290-294 ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 290-294)" _sas_scan.detc_id 15 _sas_scan.id 1307 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1268 _sas_scan.result_id 1307 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1268 _sas_sample.name "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 290-294)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1268 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 2.893940E+01 1.367814E+01 1307 2 5.968380E-03 3.453080E+01 8.134738E+00 1307 3 6.388220E-03 3.272080E+01 6.093912E+00 1307 4 6.808050E-03 2.860460E+01 4.609690E+00 1307 5 7.227880E-03 2.949520E+01 3.988082E+00 1307 6 7.647710E-03 2.684220E+01 3.091324E+00 1307 7 8.067540E-03 2.667770E+01 2.756174E+00 1307 8 8.487370E-03 2.481020E+01 2.420986E+00 1307 9 8.907200E-03 2.398530E+01 2.274680E+00 1307 10 9.327030E-03 2.172874E+01 1.912414E+00 1307 11 9.746860E-03 2.126170E+01 1.805386E+00 1307 12 1.016670E-02 2.088844E+01 1.714260E+00 1307 13 1.058650E-02 2.095512E+01 1.543754E+00 1307 14 1.100630E-02 2.106736E+01 1.505652E+00 1307 15 1.142620E-02 1.843482E+01 1.300192E+00 1307 16 1.184600E-02 1.742946E+01 1.211034E+00 1307 17 1.226580E-02 1.611058E+01 1.300926E+00 1307 18 1.268570E-02 1.700364E+01 1.093856E+00 1307 19 1.310550E-02 1.648516E+01 1.122794E+00 1307 20 1.352530E-02 1.501588E+01 1.036877E+00 1307 21 1.394520E-02 1.431680E+01 9.664524E-01 1307 22 1.436500E-02 1.331516E+01 9.915070E-01 1307 23 1.478480E-02 1.288296E+01 8.726122E-01 1307 24 1.520460E-02 1.261454E+01 9.371046E-01 1307 25 1.562450E-02 1.192512E+01 7.640012E-01 1307 26 1.604430E-02 1.224634E+01 7.686250E-01 1307 27 1.646410E-02 1.068826E+01 7.177388E-01 1307 28 1.688400E-02 1.079226E+01 7.037582E-01 1307 29 1.730380E-02 1.022942E+01 6.907500E-01 1307 30 1.772360E-02 9.571040E+00 6.323924E-01 1307 31 1.814350E-02 9.143900E+00 6.170462E-01 1307 32 1.856330E-02 8.425040E+00 5.955854E-01 1307 33 1.898310E-02 8.508900E+00 6.033552E-01 1307 34 1.940290E-02 8.237280E+00 5.768458E-01 1307 35 1.982280E-02 7.843340E+00 5.638574E-01 1307 36 2.024260E-02 7.827680E+00 5.553468E-01 1307 37 2.066240E-02 7.106600E+00 5.474050E-01 1307 38 2.108230E-02 6.972740E+00 5.265504E-01 1307 39 2.150210E-02 6.759300E+00 5.161942E-01 1307 40 2.192190E-02 6.420420E+00 4.874490E-01 1307 41 2.234180E-02 6.472500E+00 5.035886E-01 1307 42 2.276160E-02 5.820820E+00 4.696150E-01 1307 43 2.318140E-02 6.001880E+00 4.589580E-01 1307 44 2.360130E-02 5.630940E+00 4.540920E-01 1307 45 2.402110E-02 5.353120E+00 4.463474E-01 1307 46 2.444090E-02 5.378860E+00 4.346284E-01 1307 47 2.486070E-02 5.087020E+00 4.358580E-01 1307 48 2.528060E-02 4.772240E+00 4.123402E-01 1307 49 2.570040E-02 4.528520E+00 4.251110E-01 1307 50 2.612020E-02 4.100780E+00 4.095660E-01 1307 51 2.654010E-02 4.191820E+00 3.917548E-01 1307 52 2.695990E-02 3.759940E+00 3.839310E-01 1307 53 2.737970E-02 3.634320E+00 4.089554E-01 1307 54 2.779960E-02 3.743740E+00 3.771460E-01 1307 55 2.821940E-02 3.775420E+00 3.896036E-01 1307 56 2.863920E-02 3.449660E+00 3.903798E-01 1307 57 2.905900E-02 3.340060E+00 3.755148E-01 1307 58 2.947890E-02 3.209400E+00 3.827562E-01 1307 59 2.989870E-02 3.204060E+00 3.706878E-01 1307 60 3.031850E-02 3.177860E+00 3.740876E-01 1307 61 3.073840E-02 2.886540E+00 3.680612E-01 1307 62 3.115820E-02 2.823620E+00 3.609746E-01 1307 63 3.157800E-02 3.006180E+00 3.702242E-01 1307 64 3.199790E-02 2.607540E+00 3.609074E-01 1307 65 3.241770E-02 2.654580E+00 3.595204E-01 1307 66 3.283750E-02 2.623200E+00 3.591366E-01 1307 67 3.325730E-02 2.889920E+00 3.634626E-01 1307 68 3.367720E-02 2.335480E+00 3.559258E-01 1307 69 3.409700E-02 2.419100E+00 3.502484E-01 1307 70 3.451680E-02 2.350740E+00 3.655030E-01 1307 71 3.493670E-02 2.383640E+00 3.561076E-01 1307 72 3.535650E-02 2.513360E+00 3.585080E-01 1307 73 3.577630E-02 2.156420E+00 3.386696E-01 1307 74 3.619620E-02 2.055700E+00 3.446654E-01 1307 75 3.661600E-02 1.955420E+00 3.451044E-01 1307 76 3.703580E-02 1.769360E+00 3.324372E-01 1307 77 3.745560E-02 2.018740E+00 3.339550E-01 1307 78 3.787550E-02 1.888980E+00 3.326332E-01 1307 79 3.829530E-02 2.083480E+00 3.256172E-01 1307 80 3.871510E-02 1.938040E+00 3.251542E-01 1307 81 3.913500E-02 1.719480E+00 3.122476E-01 1307 82 3.955480E-02 1.889920E+00 3.198662E-01 1307 83 3.997460E-02 1.993540E+00 3.118224E-01 1307 84 4.039450E-02 1.612180E+00 3.098210E-01 1307 85 4.081430E-02 1.783480E+00 2.980080E-01 1307 86 4.123410E-02 1.969600E+00 3.027538E-01 1307 87 4.165390E-02 1.436840E+00 2.988232E-01 1307 88 4.207380E-02 1.487920E+00 2.909088E-01 1307 89 4.249360E-02 1.659540E+00 2.971336E-01 1307 90 4.291340E-02 1.539900E+00 2.930416E-01 1307 91 4.333330E-02 1.516820E+00 2.883286E-01 1307 92 4.375310E-02 1.443380E+00 2.847818E-01 1307 93 4.417290E-02 1.303000E+00 2.819962E-01 1307 94 4.459280E-02 1.208820E+00 2.768628E-01 1307 95 4.501260E-02 1.151240E+00 2.690860E-01 1307 96 4.543240E-02 1.141140E+00 2.741840E-01 1307 97 4.585220E-02 1.484760E+00 2.728486E-01 1307 98 4.627210E-02 1.414960E+00 2.696452E-01 1307 99 4.669190E-02 1.405940E+00 2.666978E-01 1307 100 4.711170E-02 1.456060E+00 2.664720E-01 1307 101 4.753160E-02 1.287740E+00 2.637674E-01 1307 102 4.795140E-02 1.286000E+00 2.680868E-01 1307 103 4.837120E-02 1.108460E+00 2.611590E-01 1307 104 4.879110E-02 9.945000E-01 2.606108E-01 1307 105 4.921090E-02 1.068440E+00 2.580548E-01 1307 106 4.963070E-02 1.169540E+00 2.543626E-01 1307 107 5.005050E-02 8.633400E-01 2.556064E-01 1307 108 5.047040E-02 1.091920E+00 2.517968E-01 1307 109 5.089020E-02 9.461598E-01 2.526114E-01 1307 110 5.131000E-02 1.006660E+00 2.442232E-01 1307 111 5.172990E-02 9.753998E-01 2.491226E-01 1307 112 5.214970E-02 1.170320E+00 2.508048E-01 1307 113 5.256950E-02 8.148404E-01 2.494628E-01 1307 114 5.298940E-02 9.932398E-01 2.410152E-01 1307 115 5.340920E-02 8.029198E-01 2.437190E-01 1307 116 5.382900E-02 9.346800E-01 2.419344E-01 1307 117 5.424890E-02 8.638396E-01 2.413642E-01 1307 118 5.466870E-02 7.936800E-01 2.400742E-01 1307 119 5.508850E-02 8.962200E-01 2.360124E-01 1307 120 5.550830E-02 9.950600E-01 2.389414E-01 1307 121 5.592820E-02 8.241396E-01 2.329008E-01 1307 122 5.634800E-02 7.623000E-01 2.344062E-01 1307 123 5.676780E-02 8.241800E-01 2.372360E-01 1307 124 5.718770E-02 8.073394E-01 2.328012E-01 1307 125 5.760750E-02 9.775002E-01 2.332066E-01 1307 126 5.802730E-02 8.181600E-01 2.278904E-01 1307 127 5.844720E-02 8.369806E-01 2.321098E-01 1307 128 5.886700E-02 7.482196E-01 2.293440E-01 1307 129 5.928680E-02 8.774200E-01 2.280238E-01 1307 130 5.970660E-02 7.034596E-01 2.293142E-01 1307 131 6.012650E-02 6.232400E-01 2.252986E-01 1307 132 6.054630E-02 6.793804E-01 2.207978E-01 1307 133 6.096610E-02 7.647600E-01 2.233052E-01 1307 134 6.138600E-02 7.586404E-01 2.237962E-01 1307 135 6.180580E-02 5.507804E-01 2.198794E-01 1307 136 6.222560E-02 8.905402E-01 2.182474E-01 1307 137 6.264550E-02 7.214194E-01 2.210768E-01 1307 138 6.306530E-02 7.045000E-01 2.194826E-01 1307 139 6.348510E-02 8.831200E-01 2.178874E-01 1307 140 6.390490E-02 7.631600E-01 2.219188E-01 1307 141 6.432480E-02 5.726200E-01 2.144300E-01 1307 142 6.474460E-02 7.327402E-01 2.180486E-01 1307 143 6.516440E-02 8.096600E-01 2.167072E-01 1307 144 6.558430E-02 7.065000E-01 2.166344E-01 1307 145 6.600410E-02 5.035798E-01 2.135182E-01 1307 146 6.642390E-02 5.702600E-01 2.105476E-01 1307 147 6.684380E-02 5.523800E-01 2.102558E-01 1307 148 6.726360E-02 4.840602E-01 2.073172E-01 1307 149 6.768340E-02 5.452002E-01 2.113708E-01 1307 150 6.810320E-02 5.204402E-01 2.078476E-01 1307 151 6.852310E-02 5.797602E-01 2.034396E-01 1307 152 6.894290E-02 5.203994E-01 2.082934E-01 1307 153 6.936270E-02 5.146602E-01 2.050514E-01 1307 154 6.978260E-02 8.022402E-01 2.071162E-01 1307 155 7.020240E-02 6.593602E-01 2.029422E-01 1307 156 7.062220E-02 5.045204E-01 2.056970E-01 1307 157 7.104210E-02 6.198200E-01 2.075124E-01 1307 158 7.146190E-02 3.544200E-01 2.015950E-01 1307 159 7.188170E-02 5.023596E-01 2.055758E-01 1307 160 7.230160E-02 4.535198E-01 1.966378E-01 1307 161 7.272140E-02 4.502996E-01 2.034808E-01 1307 162 7.314120E-02 5.045000E-01 1.983318E-01 1307 163 7.356100E-02 4.061604E-01 1.983160E-01 1307 164 7.398090E-02 5.998998E-01 2.004250E-01 1307 165 7.440070E-02 4.843202E-01 1.925946E-01 1307 166 7.482050E-02 4.477600E-01 1.992414E-01 1307 167 7.524040E-02 4.485400E-01 1.947232E-01 1307 168 7.566020E-02 6.116796E-01 1.974158E-01 1307 169 7.608000E-02 4.614198E-01 2.014536E-01 1307 170 7.649980E-02 5.448200E-01 1.941342E-01 1307 171 7.691970E-02 4.932400E-01 1.924354E-01 1307 172 7.733950E-02 4.851600E-01 1.933156E-01 1307 173 7.775930E-02 6.562404E-01 1.911344E-01 1307 174 7.817920E-02 5.119000E-01 1.979932E-01 1307 175 7.859900E-02 5.573800E-01 1.925526E-01 1307 176 7.901880E-02 4.887598E-01 1.920708E-01 1307 177 7.943870E-02 4.218398E-01 1.923336E-01 1307 178 7.985850E-02 3.805398E-01 1.906938E-01 1307 179 8.027830E-02 4.674804E-01 1.940126E-01 1307 180 8.069810E-02 4.889998E-01 1.890782E-01 1307 181 8.111800E-02 5.339600E-01 1.960410E-01 1307 182 8.153780E-02 3.604397E-01 1.882060E-01 1307 183 8.195760E-02 3.641400E-01 1.910592E-01 1307 184 8.237750E-02 3.877800E-01 1.930520E-01 1307 185 8.279730E-02 5.909598E-01 1.887112E-01 1307 186 8.321710E-02 2.520002E-01 1.873566E-01 1307 187 8.363700E-02 3.256198E-01 1.937494E-01 1307 188 8.405680E-02 5.323800E-01 1.980376E-01 1307 189 8.447660E-02 4.303001E-01 1.983478E-01 1307 190 8.489650E-02 3.946600E-01 2.003110E-01 1307 191 8.531630E-02 2.800200E-01 2.000678E-01 1307 192 8.573610E-02 4.260006E-01 2.014304E-01 1307 193 8.615590E-02 4.371200E-01 2.070548E-01 1307 194 8.657580E-02 3.948202E-01 2.008760E-01 1307 195 8.699560E-02 4.282408E-01 2.062284E-01 1307 196 8.741540E-02 3.365602E-01 2.022572E-01 1307 197 8.783530E-02 2.890200E-01 2.017748E-01 1307 198 8.825510E-02 3.601599E-01 2.059934E-01 1307 199 8.867490E-02 4.166802E-01 2.089572E-01 1307 200 8.909480E-02 2.315799E-01 2.028308E-01 1307 201 8.951460E-02 4.316200E-01 2.084924E-01 1307 202 8.993440E-02 4.351196E-01 2.055012E-01 1307 203 9.035420E-02 3.822406E-01 2.094704E-01 1307 204 9.077410E-02 4.663794E-01 2.048954E-01 1307 205 9.119390E-02 4.967400E-01 2.060418E-01 1307 206 9.161370E-02 3.316000E-01 2.034804E-01 1307 207 9.203360E-02 4.633200E-01 2.090580E-01 1307 208 9.245340E-02 3.177802E-01 2.032470E-01 1307 209 9.287320E-02 3.669608E-01 2.095212E-01 1307 210 9.329310E-02 3.321596E-01 2.113818E-01 1307 211 9.371290E-02 4.510396E-01 2.159740E-01 1307 212 9.413270E-02 2.860794E-01 2.097816E-01 1307 213 9.455250E-02 3.606400E-01 2.083412E-01 1307 214 9.497240E-02 3.208800E-01 2.060774E-01 1307 215 9.539220E-02 3.691603E-01 2.044580E-01 1307 216 9.581200E-02 3.613400E-01 2.055796E-01 1307 217 9.623190E-02 3.202200E-01 2.041718E-01 1307 218 9.665170E-02 3.570600E-01 2.029258E-01 1307 219 9.707150E-02 3.567398E-01 2.021612E-01 1307 220 9.749140E-02 2.841596E-01 1.985812E-01 1307 221 9.791120E-02 3.753200E-01 1.979902E-01 1307 222 9.833100E-02 2.681200E-01 1.997974E-01 1307 223 9.875080E-02 4.464406E-01 2.022626E-01 1307 224 9.917070E-02 2.674407E-01 2.002574E-01 1307 225 9.959050E-02 2.927601E-01 1.978378E-01 1307 226 1.000100E-01 2.813595E-01 1.952408E-01 1307 227 1.004300E-01 2.922605E-01 1.987048E-01 1307 228 1.008500E-01 1.500199E-01 1.908106E-01 1307 229 1.012700E-01 3.793606E-01 2.036664E-01 1307 230 1.016900E-01 3.851401E-01 1.936454E-01 1307 231 1.021090E-01 3.912400E-01 1.981846E-01 1307 232 1.025290E-01 3.119599E-01 1.943068E-01 1307 233 1.029490E-01 2.674596E-01 1.876806E-01 1307 234 1.033690E-01 2.486401E-01 1.970158E-01 1307 235 1.037890E-01 2.161593E-01 1.902928E-01 1307 236 1.042090E-01 3.217794E-01 1.910186E-01 1307 237 1.046280E-01 2.825001E-01 1.957436E-01 1307 238 1.050480E-01 1.871806E-01 1.906666E-01 1307 239 1.054680E-01 2.887200E-01 1.907832E-01 1307 240 1.058880E-01 2.659801E-01 1.915870E-01 1307 241 1.063080E-01 1.625203E-01 1.928230E-01 1307 242 1.067280E-01 1.804602E-01 1.899654E-01 1307 243 1.071470E-01 1.487202E-01 1.859928E-01 1307 244 1.075670E-01 2.025405E-01 1.930748E-01 1307 245 1.079870E-01 2.940794E-01 1.911122E-01 1307 246 1.084070E-01 2.606804E-01 1.907644E-01 1307 247 1.088270E-01 2.005396E-01 1.897172E-01 1307 248 1.092470E-01 2.090999E-01 1.901240E-01 1307 249 1.096660E-01 2.849793E-01 1.871082E-01 1307 250 1.100860E-01 2.838996E-01 1.868842E-01 1307 251 1.105060E-01 7.227976E-02 1.875934E-01 1307 252 1.109260E-01 2.191998E-01 1.871784E-01 1307 253 1.113460E-01 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