data_SASDCU6_MAIN # _sas_result.id 667 _sas_result.SASBDB_code SASDCU6 _sas_result.experimental_MW 105.000 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR 0.0220000000 _sas_result.I0_from_PR_error 0.0001300000 _sas_result.I0_from_Guinier 0.0226300000 _sas_result.I0_from_Guinier_error 0.0002900000 _sas_result.Rg_from_PR 5.035 _sas_result.Rg_from_PR_error 0.180 _sas_result.Rg_from_Guinier 5.213 _sas_result.Rg_from_Guinier_error 0.152 _sas_result.D_max 12.80 _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 1 _sas_result.Guinier_point_max 8 _sas_result.type_of_curve single_conc _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;12N12 nucleosomes in the presence of the chromatin remodeler Chd1 in 60% sucrose and no nucleotides added (apo). The complex formed contains two Chd1 proteins bound to each nucleosome. Due to contrast matching between the solvent and histone proteins, the measured signal is dominated by the DNA component. The models were generated using 3D-DART and custom scripts written in Pymol. The percentage-weighting of each model in the ensemble ('XXXperc') is noted in the following model key/list that sequentially corresponds to models displayed in this entry ordered from top-to-bottom: SASDCU6_fit1_model1 = Chd1_12N12_apo_01_wrapped_27perc; SASDCU6_fit1_model2 = Chd1_12N12_apo_02_extreme_09perc; SASDCU6_fit1_model3 = Chd1_12N12_apo_03_wrapped_36perc; SASDCU6_fit1_model4 = Chd1_12N12_apo_04_inplane_27perc. ; # _sas_scan.title "Chd1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose without any nucleotides added (Apo)" _sas_scan.detc_id 39 _sas_scan.id 667 _sas_scan.measurement_date 2015-10-24 _sas_scan.storage_temperature 25.0 _sas_scan.cell_temperature 25.0 _sas_scan.exposure_time 10.000 _sas_scan.number_of_frames 40 _sas_scan.unit 1/A _sas_scan.qmin 0.0160 _sas_scan.qmax 0.2600 _sas_scan.sample_id 625 _sas_scan.result_id 667 _sas_scan.beam_id 60 # _sas_beam.id 60 _sas_beam.instrument_name "Cornell High Energy Synchrotron Source (CHESS) G1" _sas_beam.instrument_city "Ithaca, NY" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.110900 _sas_beam.dist_spec_to_detc 2.08000 # _sas_detc.id 39 _sas_detc.name "Pilatus 200K" _sas_detc.type Pilatus _sas_detc.pixsize_x 172.000 # _sas_sample.id 625 _sas_sample.name "Chd1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose without any nucleotides added (Apo)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 1.88 _sas_sample.concentration_method . loop_ _sas_sample.entity_id 768 769 770 771 772 773 # _sas_buffer.id 387 _sas_buffer.name "10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.800 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 625 # loop_ _entity.id _entity.type _entity.pdbx_description _entity.formula_weight _entity.pdbx_number_of_molecules 768 protein "Chromodomain-helicase-DNA-binding protein 1" 133.115 2 769 DNA "169 bp DNA (145 bp Widom 601, flanked by 12bp DNA)" 52.468 1 770 protein "Histone H2A type 1" 13.966 1 771 protein "Histone H2B 1.1" 13.934 1 772 protein "Histone H3.2" 15.388 1 773 protein "Histone H4" 11.367 1 # loop_ _entity_name_com.entity_id _entity_name_com.name 768 Chd1 769 DNA 770 H2A 771 H2B 772 H3 773 H4 # loop_ _entity_poly.entity_id _entity_poly.pdbx_seq_one_letter_code 768 ;GPQSTVKIPTRFSNRQNKTVNYNIDYSDDDLLESEDDYGSEEALSEENVH EASANPQPEDFHGIDIVINHRLKTSLEEGKVLEKTVPDLNNCKENYEFLI KWTDESHLHNTWETYESIGQVRGLKRLDNYCKQFIIEDQQVRLDPYVTAE DIEIMDMERERRLDEFEEFHVPERIIDSQRASLEDGTSQLQYLVKWRRLN YDEATWENATDIVKLAPEQVKHFQNRENSKILPQYSSNYTSQRPRFEKLS VQPPFIKGGELRDFQLTGINWMAFLWSKGDNGILADEMGLGKTVQTVAFI SWLIFARRQNGPHIIVVPLSTMPAWLDTFEKWAPDLNCICYMGNQKSRDT IREYEFYTNPRAKGKKTMKFNVLLTTYEYILKDRAELGSIKWQFMAVDEA HRLKNAESSLYESLNSFKVANRMLITGTPLQNNIKELAALVNFLMPGRFT IDQEIDFENQDEEQEEYIHDLHRRIQPFILRRLKKDVEKSLPSKTERILR VELSDVQTEYYKNILTKNYSALTAGAKGGHFSLLNIMNELKKASNHPYLF DNAEERVLQKFGDGKMTRENVLRGLIMSSGKMVLLDQLLTRLKKDGHRVL IFSQMVRMLDILGDYLSIKGINFQRLDGTVPSAQRRISIDHFNSPDSNDF VFLLSTRAGGLGINLMTADTVVIFDSDWNPQADLQAMARAHRIGQKNHVM VYRLVSKDTVEEEVLERARKKMILEYAIISLGVTDGNKYTKKNEPNAGEL SAILKFGAGNMFTATDNQKKLEDLNLDDVLNHAEDHVTTPDLGESHLGGE EFLKQFEVTDYKADIDWDDIIPEEELKKLQDEEQKRKDEEYVKEQLEMMN RRDNALKKIKNSVNGDGTAANSDSDDDSTSRSSRRRARANDMDSIGESEV RALYKAILKFGNLKEILDELIADGTLPVKSFEKYGETYDEMMEAAKDCVH EEEKNRKEILEKLEKHATAYRAKLKSGEIKAENQPKDNPLTRLSLKKREK KAVLFNFKGVKSLNAESLLSRVEDLKYLKNLINSNYKDDPLKFSLGNNTP KPVQNWSSNWTKEEDEKLLIGVFKYGYGSWTQIRDDPFLGITDKIFLNEV HNPVAKKSASSSDTTPTPSKKGKGITGSSKKVPGAIHLGRRVDYLLSFLR GGLNTKSPS ; 769 CTTTCAGCTGATATCGATGTATATATCTGACACGTGCCTGGAGACTAGGGAGTAATCCCCTTGGCGGTTAAAACGCGGGGGACAGCGCGTACGTGCGTTTAAGCGGTGCTAGAGCTGTCTACGACCAATTGAGCGGCCTCGGCACCGGGATCTTGATATCAGCTGAAAG 770 ;MSGRGKQGGKTRAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLT AEILELAGNAARDNKKTRIIPRHLQLAVRNDEELNKLLGGVTIAQGGVLPNIQSVLLPKK TESAKSAKSK ; 771 ;MPEPAKSAPAPKKGSKKAVTKTQKKDGKKRRKSRKESYAIYVYKVLKQVHPDTGISSKAM SIMNSFVNDVFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVT KYTSAK ; 772 ;MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTE LLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTI MPKDIQLARRIRGERA ; 773 ;MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK VFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG ; # loop_ _entity_src_gen.entity_id _entity_src_gen.gene_src_common_name 768 "Saccharomyces cerevisiae (strain ATCC 204508 / S288c)" 769 . 770 "Xenopus laevis" 771 "Xenopus laevis" 772 "Xenopus laevis" 773 "Xenopus laevis" # loop_ _struct_ref.id _struct_ref.entity_id _struct_ref.db_name _struct_ref.db_code _struct_ref.details 1 768 UniProt P32657 http://www.uniprot.org/uniprot/P32657 2 769 UniProt . http://www.uniprot.org/uniprot/P06897 3 770 UniProt P06897 http://www.uniprot.org/uniprot/P02281 4 771 UniProt P02281 http://www.uniprot.org/uniprot/P84233 5 772 UniProt P84233 http://www.uniprot.org/uniprot/P62799