{ "code": "SASDVU6", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation.", "publication": { "title": "Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation.", "author_list": "Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN", "journal": "Nucleic Acids Res", "doi": "10.1093/nar/gkaf502", "pmid": "40503686", "published_date": "2025 Jun 6" }, "status": "released", "submitted_date": "2023-09-21", "released_date": "2025-02-10" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDVU6.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDVU6.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVU6_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVU6_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVU6_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVU6_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDVU6.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Eiger 4M", "resolution": 75.0 }, "name": "Diamond Light Source", "city": "Didcot", "country": "UK", "beamline_name": "B21", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Nucleoprotein", "short_name": "N1-245", "sequence": "GPMSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHG\r\nKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAG\r\nLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGS\r\nQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQ\r\nQQGQTWSHPQFEK", "organism": "Severe acute respiratory syndrome coronavirus 2", "uniprot_code": "P0DTC9", "uniprot_range_first": 1, "uniprot_range_last": 245, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHG\nKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAG\nLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGS\nQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQ\nQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKH\nWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAY\nKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA", "mw": 27.388, "total_mw": 54.776, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "Strep(II)-tagged N-protein 1-245. The protein construct used for SAXS contains an additional two amino acids at the N-terminus (GP) as well as the VWSHPQFEK C-terminal tag." } ], "buffer": { "name": "100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 8.0, "deuteration": null }, "purity_method": null, "name": "SARS-CoV-2 N-protein (N1-245; amino acids 1-245): 167 µM", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Oeiras, Portugal", "full_name": "Instituto de Tecnologia Química e Biológica António Xavier, Universidade de Lisboa", "webpage": "http://www.itqb.unl.pt" } ], "contributor_name": "Guillem", "contributor_surname": "Hernandez", "orcid": "https://orcid.org/0000-0003-4438-5611" } ], "concentration_method": null, "concentration_unit": null, "date": "2022-05-13", "storage_temperature": 10.0, "cell_temperature": 10.0, "exposure_time": 2.0, "number_of_frames": 10, "wavelength": 0.0954, "sample_detector_distance": 3.69, "concentration_min": null, "concentration_max": 4.6, "sample_volume": null, "flow_rate": null, "s_min": 0.045, "s_max": 3.4, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of SARS-CoV-2 N-protein (amino acids 1-245) in 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 8 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.0954 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.60 mg/ml was measured at 10°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 54.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 0.4605, "pddf_i0_error": null, "guinier_i0": 0.466054, "guinier_i0_error": null, "pddf_rg": 5.2, "pddf_rg_error": 0.01, "guinier_rg": 5.17, "guinier_rg_error": 0.169, "pddf_dmax": 20.5, "pddf_dmax_error": 0.5, "porod_volume": 121.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 6, "guinier_point_last": 113, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDVU6_datloglog_img.png", "symmetry": null, "last_modified": "2025-06-16T15:30:57.949374+02:00", "bragg_peak": [] }