{
  "code": "SASDWU6",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "A structural basis for chaperone repression of stress signaling from the endoplasmic reticulum.",
    "publication": {
      "title": "A structural basis for chaperone repression of stress signaling from the endoplasmic reticulum.",
      "author_list": "Neidhardt L, Tung J, Kuchersky M, Milczarek J, Kargas V, Stott K, Rosenzweig R, Ron D, Yan Y",
      "journal": "Mol Cell",
      "doi": "10.1016/j.molcel.2025.09.032",
      "pmid": "41135511",
      "published_date": "2025 Oct 23"
    },
    "status": "released",
    "submitted_date": "2025-01-24",
    "released_date": "2025-10-23"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDWU6.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDWU6.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWU6_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWU6_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWU6_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWU6_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDWU6.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Eiger 4M",
        "resolution": 75.0
      },
      "name": "Diamond Light Source",
      "city": "Didcot",
      "country": "UK",
      "beamline_name": "B21",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Anterior gradient protein 2 homolog",
          "short_name": "AGR2",
          "sequence": "PQTLSRGWGDQLIWTQTYEEALYKSKTSNKPLMIIHHLDECPHSQALKKVFAENKEIQKLAEQFVLLNLVYETTDKHLSPDGQYVPRIMFVDPSLTVRADITGRYSNRLYAYEPADTALLLDNMKKALKLL",
          "organism": "Homo sapiens",
          "uniprot_code": "O95994",
          "uniprot_range_first": 41,
          "uniprot_range_last": 171,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MEKIPVSAFLLLVALSYTLARDTTVKPGAKKDTKDSRPKLPQTLSRGWGDQLIWTQTYEE\nALYKSKTSNKPLMIIHHLDECPHSQALKKVFAENKEIQKLAEQFVLLNLVYETTDKHLSP\nDGQYVPRIMFVDPSLTVRADITGRYSNRLYAYEPADTALLLDNMKKALKLLKTEL",
          "mw": 15.122,
          "total_mw": 30.244,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Required for MUC2 post-transcriptional synthesis and secretion."
        }
      ],
      "buffer": {
        "name": "20 mM Tris pH7.4, 150 mM NaCl, 1% glycerol, 0.2 mM TCEP",
        "concentration_unit": null,
        "comment": "The buffer for SEC runs was the same as the sample buffer.",
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Human anterior gradient protein 2 (AGR2)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "University of Cambridge",
            "webpage": null
          }
        ],
        "contributor_name": "Yahui",
        "contributor_surname": "Yan",
        "orcid": "https://orcid.org/0000-0001-6934-9874"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2024-05-05",
    "storage_temperature": 15.0,
    "cell_temperature": 15.0,
    "exposure_time": 1.0,
    "number_of_frames": 30,
    "wavelength": 0.09464,
    "sample_detector_distance": 3.6929,
    "concentration_min": null,
    "concentration_max": 46.0,
    "sample_volume": 35.0,
    "flow_rate": 0.06,
    "s_min": 0.045,
    "s_max": 3.4,
    "total_exposure_time": null,
    "seccolumn": 13
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDWU6_fit1_model1_img.png",
          "software": "MultiFoXS",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDWU6_fit1_model1.pdb",
          "model_mw": 30.03,
          "bead_radius": null,
          "log": null,
          "symmetry": null,
          "comment": null,
          "user": 1180
        },
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDWU6_fit1_model2_img.png",
          "software": "MultiFoXS",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDWU6_fit1_model2.pdb",
          "model_mw": 15.02,
          "bead_radius": null,
          "log": null,
          "symmetry": null,
          "comment": null,
          "user": 1180
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDWU6_fit1_fitfoxs_img.png",
      "software": "MultiFoXS",
      "chi_square_value": 4.229369,
      "p_value": 0.0,
      "fit_residual_plot": "SASDWU6_fit1_fitfoxsresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDWU6_fit1.dat",
      "fit_log": null,
      "software_version": null,
      "description": ""
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of anterior gradient protein 2 (AGR2) in 20 mM Tris pH7.4, 150 mM NaCl, 1% glycerol, 0.2 mM TCEP, were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.09464 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 35.00 μl sample at 46 mg/ml was injected at a 0.06 ml/min flow rate onto a GE Superdex 75 Increase 3.2/300 column at 15°C. 30 successive 1 second frames were collected through the sample elution peak (from a total of 600 frames collected throughout the entire SEC run). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": "1/cm",
  "experimental_mw": 24.4,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 18.7,
  "porod_mw_error": null,
  "pddf_i0": 0.1785,
  "pddf_i0_error": null,
  "guinier_i0": 0.177626,
  "guinier_i0_error": null,
  "pddf_rg": 1.98,
  "pddf_rg_error": 0.018,
  "guinier_rg": 1.955,
  "guinier_rg_error": 0.011,
  "pddf_dmax": 6.27,
  "pddf_dmax_error": null,
  "porod_volume": 29.9,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 224,
  "guinier_point_last": 473,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDWU6_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2025-10-27T12:08:22.283589+01:00",
  "bragg_peak": []
}