{ "code": "SASDCU8", "status": "Published", "type_of_curve": "Other", "angular_unit": "1/nm", "project": { "title": "Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors.", "publication": { "title": "Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors.", "author_list": "de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V", "journal": "Mol Cell", "doi": "10.1016/j.molcel.2018.02.022", "pmid": "29547724", "published_date": "2018 Mar 15" }, "status": "released", "submitted_date": "2017-11-06", "released_date": "2018-03-20" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDCU8.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDCU8.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCU8_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCU8_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCU8_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCU8_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDCU8.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 2M", "resolution": null }, "name": "PETRA III", "city": "DESY; Hamburg", "country": "Germany", "beamline_name": "EMBL P12", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "hypothetical protein CTHT_0072540", "short_name": null, "sequence": "SLSEGLVEHVNEVQQQLMKTRKKRPI\r\nPQGWATADDVAALQQVAYTDLNVTQASSLDLENECAAVGGLDGKLDIYSVVANKVERTLD\r\nIGEPVTATEWTGTKVVIGTAKGWVKVYDAGRESATFQTHAGPVTGLAVHPGGRILASVGV\r\nDKSFVFYDLETGERVARGYADAALTTCAFHPDGNLFAAGTQTGHILVFHTTTLEQAESFP\r\nLGTPIQALAFSENGFWFAATGKGTSSVTIFDLRKSGAAAAVKELQTGEVLSISWDYTGQY\r\nLATGGGTGVTVQMYTKATKSWSEPVRLGMPVVGVKWGGEAKRLVVVSREGVVSVLGKKEE", "organism": "Chaetomium thermophilum", "uniprot_code": "G0SFY0", "uniprot_range_first": 155, "uniprot_range_last": 480, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MLCALSGEIPEEPVVSKKTGVLFEKRLILKYLEEHNNIEPGTTEELDPETDLLPIKTSRV\nVRPRPPNFTSIPSLLKAFQDEWDALVLETYTTREQLARVREELATALYQHDAAVRVIARL\nTRERDEAREALARLTVTGAAPAAQNGEAMAVDSESLSEGLVEHVNEVQQQLMKTRKKRPI\nPQGWATADDVAALQQVAYTDLNVTQASSLDLENECAAVGGLDGKLDIYSVVANKVERTLD\nIGEPVTATEWTGTKVVIGTAKGWVKVYDAGRESATFQTHAGPVTGLAVHPGGRILASVGV\nDKSFVFYDLETGERVARGYADAALTTCAFHPDGNLFAAGTQTGHILVFHTTTLEQAESFP\nLGTPIQALAFSENGFWFAATGKGTSSVTIFDLRKSGAAAAVKELQTGEVLSISWDYTGQY\nLATGGGTGVTVQMYTKATKSWSEPVRLGMPVVGVKWGGEAKRLVVVSREGVVSVLGKKEE", "mw": 34.583, "total_mw": 34.583, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "Uncharacterized protein CTHT_0072540 (WD40) from Chaetomium thermophilum", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "EMBL-Hamburg", "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany", "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation", "webpage": "http://www.embl-hamburg.de/index.php" } ], "contributor_name": "Tales", "contributor_surname": "Rocha de Moura", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2013-07-19", "storage_temperature": null, "cell_temperature": 25.0, "exposure_time": 1.0, "number_of_frames": 20, "wavelength": 0.124, "sample_detector_distance": 3.1, "concentration_min": 1.5, "concentration_max": 5.0, "sample_volume": null, "flow_rate": null, "s_min": 0.183, "s_max": 2.811, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDCU8_fit1_model1_img.png", "software": "SASREF", "pdb_link": [], "model_title": null, "type_of_model": "atomic", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDCU8_fit1_model1.pdb", "model_mw": 34.34, "bead_radius": 1.9, "log": null, "symmetry": "", "comment": "", "user": 227 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCU8_fit1_fit_img.png", "software": "SASREF", "chi_square_value": 1.331, "p_value": 0.388158, "fit_residual_plot": "fit4columns1039_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDCU8_fit1.fit", "fit_log": null, "software_version": "", "description": null }, { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDCU8_fit2_model1_img.png", "software": "DAMMIN", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDCU8_fit2_model1.pdb", "model_mw": 34.258, "bead_radius": 2.0, "log": null, "symmetry": "", "comment": "", "user": 227 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCU8_fit2_fit_img.png", "software": "DAMMIN", "chi_square_value": 1.086, "p_value": 0.058107, "fit_residual_plot": "SASDCU8_fit2_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDCU8_fit2.fir", "fit_log": null, "software_version": "", "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of Uncharacterized protein CTHT_0072540 (WD40) from Chaetomium thermophilum in 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH 7.5 were collected on the P12 beam line at the PETRAIII the storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1.5 and 5 mg/ml were measured at 25°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 40.8, "experimental_mw_error": null, "guinier_i0_mw": 56.0, "guinier_i0_mw_error": null, "porod_mw": 40.8, "porod_mw_error": null, "pddf_i0": 1376.0, "pddf_i0_error": 9.0, "guinier_i0": 1477.17, "guinier_i0_error": 23.5, "pddf_rg": 2.15, "pddf_rg_error": 0.009, "guinier_rg": 2.3, "guinier_rg_error": 0.09, "pddf_dmax": 5.8, "pddf_dmax_error": 6.0, "porod_volume": 68.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 97, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDCU8_datloglog_img.png", "symmetry": null, "last_modified": "2023-06-21T07:56:39.492710+02:00", "bragg_peak": [] }