data_SASDFU8_MAIN # _sas_result.id 1499 _sas_result.SASBDB_code SASDFU8 _sas_result.experimental_MW 105.000 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod 81.000 _sas_result.MW_Porod_error . _sas_result.I0_from_PR 280.7000000000 _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 279.1900000000 _sas_result.I0_from_Guinier_error 1.0400000000 _sas_result.Rg_from_PR 4.040 _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 3.960 _sas_result.Rg_from_Guinier_error 0.060 _sas_result.D_max 13.50 _sas_result.Porod_volume 130.00 _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 21 _sas_result.Guinier_point_max 117 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments "SEC-SAXS analysis was performed in parallel with right-angle laser light scattering (RALLS) and refractive index measurements at room temperature. The Rg-correlation through the SEC-SAXS peak, as well as the results from coupled RALLS/RI, and alternative CORAL rigid-body refined models that fit the scattering data are included in the full entry zip archive. The quoted experimental molecular weight was determined from RALLS/RI measurements that were recorded from the same sample eluting from the column using a split-flow SEC-SAXS-light scattering configuration as described in Graewert et al., (2015) Sci. Reports. 5, 10734: doi: 10.1038/srep10734. The MW estimated directly from the SAXS data using Bayesian inference is ca. 86 kDa in the interval 79-93 kDa (Hajizadeh et al., (2018) Sci. Reports. 8(1):7204. doi: 10.1038/s41598-018-25355-2)." # _sas_scan.title "The retinoic acid receptor (RXR/RAR heterodimer) bound to the DNA response element F11r DR5." _sas_scan.detc_id 4 _sas_scan.id 1499 _sas_scan.measurement_date 2014-01-19 _sas_scan.storage_temperature 20.0 _sas_scan.cell_temperature 10.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 74 _sas_scan.unit 1/A _sas_scan.qmin 0.0020 _sas_scan.qmax 0.4512 _sas_scan.sample_id 1460 _sas_scan.result_id 1499 _sas_scan.beam_id 6 # _sas_beam.id 6 _sas_beam.instrument_name "PETRA III EMBL P12" _sas_beam.instrument_city Hamburg _sas_beam.instrument_country Germany _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.124000 _sas_beam.dist_spec_to_detc 3.00000 # _sas_detc.id 4 _sas_detc.name "Pilatus 2M" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1460 _sas_sample.name "The retinoic acid receptor (RAR-RXR heterodimer) bound to the DNA response element F11r DR5." _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 4.80 _sas_sample.concentration_method . loop_ _sas_sample.entity_id 1783 1784 1786 # _sas_buffer.id 929 _sas_buffer.name "20 mM Tris, pH 8, 150 mM NaCl, 5% v/v glycerol, 1 mM CHAPS, 4 mM MgSO4, 1 mM TCEP" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 8.000 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1460 # loop_ _entity.id _entity.type _entity.pdbx_description _entity.formula_weight _entity.pdbx_number_of_molecules 1783 protein "Retinoic acid receptor alpha, RAR" 40.786 1 1784 protein "Retinoic acid receptor RXR-alpha" 37.745 1 1786 DNA "DNA response element F11r DR5" 12.995 1 # loop_ _entity_name_com.entity_id _entity_name_com.name 1783 mRARa 1784 mRXRa 1786 "F11r DR5" # loop_ _entity_poly.entity_id _entity_poly.pdbx_seq_one_letter_code 1783 MGSSHHHHHHSSGLVPRGSHMIYKPCFVCQDKSSGYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFDVGMSKESVRNDRNKKKKEAPKPECSESYTLTPEVGELIEKVRKAHQETFPALCQLGKYTTNNSSEQRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLICGDRQDLEQPDKVDMLQEPLLEALKVYVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLD 1784 FTKHICAICGDRSSGKHYGVYSCEGCKGFFKRTVRKDLTYTCRDNKDCLIDKRQRNRCQYCRYQKCLAMGMKREAVQEERQRGKDRNENEVESTSSANEDMPVEKILEAELAVEPKTETYVEANMGLNPSSPNDPVTNICQAADKQLFTLVEWAKRIPHFSELPLDDQVILLRAGWNELLIASFSHRSIAVKDGILLATGLHVHRNSAHSAGVGAIFDRVLTELVSKMRDMQMDKTELGCLRAIVLFNPDSKGLSNPAEVEALREKVYASLEAYCKHKYPEQPGRFAKLLLRLPALRSIGLKCLEHLFFFKLIGDTPIDTFLMEMLEAPHQAT 1786 ;AGGGGTCAAGCCAGGTTCACT TCCCCAGTTCGGTCCAAGTGA ; # loop_ _entity_src_gen.entity_id _entity_src_gen.gene_src_common_name 1783 "Mus musculus" 1784 "Mus musculus" 1786 . # loop_ _struct_ref.id _struct_ref.entity_id _struct_ref.db_name _struct_ref.db_code _struct_ref.details 1 1783 UniProt P11416 http://www.uniprot.org/uniprot/P11416 2 1784 UniProt P28700 http://www.uniprot.org/uniprot/P28700