{ "code": "SASDBU9", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "Human βB2-Crystallin Forms a Face-en-Face Dimer in Solution: An Integrated NMR and SAXS Study.", "publication": { "title": "Human βB2-Crystallin Forms a Face-en-Face Dimer in Solution: An Integrated NMR and SAXS Study.", "author_list": "Xi Z, Whitley MJ, Gronenborn AM", "journal": "Structure", "doi": "10.1016/j.str.2017.02.001", "pmid": "28238532", "published_date": "2017 Mar 7" }, "status": "released", "submitted_date": "2017-01-17", "released_date": "2017-03-21" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDBU9.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDBU9.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBU9_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBU9_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBU9_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBU9_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDBU9.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 2M", "resolution": null }, "name": "Advanced Photon Source (APS), Argonne National Laboratory", "city": "Lemont, IL", "country": "USA", "beamline_name": "12-ID-B SAXS/WAXS", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Beta-crystallin B2", "short_name": null, "sequence": ">|terminally truncated human βB2-crystallin\r\nMLNPKIIIFEQENFQGHSHELNGPCPNLKETGVEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDSSDFGAPHPQVQSVRRIRDMQW", "organism": "Homo sapiens", "uniprot_code": "P43320", "uniprot_range_first": 15, "uniprot_range_last": 195, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MASDHQTQAGKPQSLNPKIIIFEQENFQGHSHELNGPCPNLKETGVEKAGSVLVQAGPWV\nGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGK\nKMEIIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDSSDFGAP\nHPQVQSVRRIRDMQWHQRGAFHPSN", "mw": 20.911, "total_mw": 41.822, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "25 mM NaPi, 5 mM DTT, 1 mM EDTA,", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 6.5, "deuteration": null }, "purity_method": null, "name": "Terminally truncated human βB2-crystallin", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Pittsburgh, PA USA", "full_name": "University of Pittsburgh", "webpage": "http://www.pitt.edu/" } ], "contributor_name": "Zhaoyong", "contributor_surname": "Xi", "orcid": null } ], "concentration_method": null, "concentration_unit": "mg/ml", "date": "2015-03-20", "storage_temperature": null, "cell_temperature": null, "exposure_time": 0.4, "number_of_frames": 30, "wavelength": 0.08856, "sample_detector_distance": 2.0, "concentration_min": null, "concentration_max": 1.75, "sample_volume": null, "flow_rate": null, "s_min": 0.323, "s_max": 4.499, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDBU9_fit1_model1_img.png", "software": "DAMMIN", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDBU9_fit1_model1.pdb", "model_mw": 31.585, "bead_radius": 2.0, "log": null, "symmetry": "", "comment": "", "user": 200 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBU9_fit1_fit_img.png", "software": "DAMMIN", "chi_square_value": 0.216, "p_value": 0.0384, "fit_residual_plot": "SASDBU9_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDBU9_fit1.fir", "fit_log": null, "software_version": "", "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "ATSAS 2.6.0", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of truncated human βB2-crystallin in 25 mM sodium phosphate, 5 mM DTT, 1 mM EDTA, pH 6.5, were collected on the 12ID-B SAXS/WAXS beam line at the Advanced Photon Source (APS, Argonne, IL, USA) using a Pilatus 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.08856 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Data were acquired over 30 successive 0.400 second frames from a sample at 1.75 mg/ml. The data were normalized to the intensity of the transmitted beam and radially averaged. The scattering of the radially-averaged solvent-blank was subtracted to produce the scattering profile displayed in this entry.", "tags": [], "intensity_unit": null, "experimental_mw": 38.6, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 38.6, "porod_mw_error": null, "pddf_i0": 0.0513, "pddf_i0_error": 0.0002, "guinier_i0": 0.051, "guinier_i0_error": 0.0004, "pddf_rg": 2.076, "pddf_rg_error": 0.012, "guinier_rg": 2.09, "guinier_rg_error": 0.019, "pddf_dmax": 6.7, "pddf_dmax_error": null, "porod_volume": 61.84, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 2, "guinier_point_last": 29, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDBU9_datloglog_img.png", "symmetry": null, "last_modified": "2023-03-02T16:54:16.648202+01:00", "bragg_peak": [] }