data_SASDFV2_MAIN # _sas_result.id 1308 _sas_result.SASBDB_code SASDFV2 _sas_result.experimental_MW 29.920 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 32.6000000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 12.090 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 6 _sas_result.Guinier_point_max 13 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73A) polymer frame no. 295-299 ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 295-299)" _sas_scan.detc_id 15 _sas_scan.id 1308 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1269 _sas_scan.result_id 1308 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1269 _sas_sample.name "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 295-299)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1269 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 1.945100E+01 1.472515E+01 1308 2 5.968380E-03 2.622920E+01 8.696430E+00 1308 3 6.388220E-03 2.875500E+01 5.952698E+00 1308 4 6.808050E-03 3.044900E+01 4.692656E+00 1308 5 7.227880E-03 2.990200E+01 3.797618E+00 1308 6 7.647710E-03 2.515960E+01 3.062088E+00 1308 7 8.067540E-03 2.413850E+01 2.728996E+00 1308 8 8.487370E-03 2.295100E+01 2.514004E+00 1308 9 8.907200E-03 2.079696E+01 2.000700E+00 1308 10 9.327030E-03 2.230892E+01 1.917652E+00 1308 11 9.746860E-03 1.969908E+01 1.685684E+00 1308 12 1.016670E-02 1.986824E+01 1.767344E+00 1308 13 1.058650E-02 1.914774E+01 1.548916E+00 1308 14 1.100630E-02 1.716694E+01 1.451746E+00 1308 15 1.142620E-02 1.728030E+01 1.323750E+00 1308 16 1.184600E-02 1.609694E+01 1.202696E+00 1308 17 1.226580E-02 1.638768E+01 1.218712E+00 1308 18 1.268570E-02 1.572194E+01 1.079574E+00 1308 19 1.310550E-02 1.503088E+01 1.096606E+00 1308 20 1.352530E-02 1.356662E+01 1.054476E+00 1308 21 1.394520E-02 1.316954E+01 1.025193E+00 1308 22 1.436500E-02 1.203126E+01 9.745886E-01 1308 23 1.478480E-02 1.187732E+01 8.346312E-01 1308 24 1.520460E-02 1.217652E+01 8.478936E-01 1308 25 1.562450E-02 1.164084E+01 8.185996E-01 1308 26 1.604430E-02 1.157416E+01 7.481540E-01 1308 27 1.646410E-02 1.029684E+01 7.131116E-01 1308 28 1.688400E-02 9.459440E+00 6.851874E-01 1308 29 1.730380E-02 9.944620E+00 6.766448E-01 1308 30 1.772360E-02 8.959060E+00 6.398864E-01 1308 31 1.814350E-02 8.718240E+00 6.153882E-01 1308 32 1.856330E-02 8.355600E+00 6.047380E-01 1308 33 1.898310E-02 8.511380E+00 5.808258E-01 1308 34 1.940290E-02 7.920840E+00 5.742464E-01 1308 35 1.982280E-02 7.623280E+00 5.316222E-01 1308 36 2.024260E-02 7.305660E+00 5.449180E-01 1308 37 2.066240E-02 7.083980E+00 5.364858E-01 1308 38 2.108230E-02 6.589980E+00 5.031728E-01 1308 39 2.150210E-02 6.386000E+00 5.116688E-01 1308 40 2.192190E-02 5.783900E+00 4.916116E-01 1308 41 2.234180E-02 6.112160E+00 4.952684E-01 1308 42 2.276160E-02 5.446940E+00 4.446308E-01 1308 43 2.318140E-02 5.821580E+00 4.618954E-01 1308 44 2.360130E-02 5.593920E+00 4.653792E-01 1308 45 2.402110E-02 4.903400E+00 4.423108E-01 1308 46 2.444090E-02 5.008640E+00 4.324626E-01 1308 47 2.486070E-02 4.931120E+00 4.188962E-01 1308 48 2.528060E-02 4.802800E+00 4.146268E-01 1308 49 2.570040E-02 4.665440E+00 4.178276E-01 1308 50 2.612020E-02 3.983200E+00 4.094002E-01 1308 51 2.654010E-02 3.892940E+00 4.060696E-01 1308 52 2.695990E-02 4.110680E+00 3.922200E-01 1308 53 2.737970E-02 3.710700E+00 4.084534E-01 1308 54 2.779960E-02 3.552560E+00 3.860120E-01 1308 55 2.821940E-02 3.758280E+00 3.928458E-01 1308 56 2.863920E-02 3.569980E+00 3.896860E-01 1308 57 2.905900E-02 3.420520E+00 3.878102E-01 1308 58 2.947890E-02 3.243740E+00 3.921938E-01 1308 59 2.989870E-02 3.013740E+00 3.734760E-01 1308 60 3.031850E-02 3.049940E+00 3.821534E-01 1308 61 3.073840E-02 2.934540E+00 3.793476E-01 1308 62 3.115820E-02 3.193960E+00 3.727240E-01 1308 63 3.157800E-02 2.862060E+00 3.812408E-01 1308 64 3.199790E-02 2.817320E+00 3.556032E-01 1308 65 3.241770E-02 2.693580E+00 3.705804E-01 1308 66 3.283750E-02 2.481860E+00 3.522376E-01 1308 67 3.325730E-02 2.496600E+00 3.721478E-01 1308 68 3.367720E-02 2.488860E+00 3.599992E-01 1308 69 3.409700E-02 1.997040E+00 3.466008E-01 1308 70 3.451680E-02 2.493260E+00 3.570356E-01 1308 71 3.493670E-02 2.298520E+00 3.406702E-01 1308 72 3.535650E-02 2.370560E+00 3.601520E-01 1308 73 3.577630E-02 2.092440E+00 3.455702E-01 1308 74 3.619620E-02 1.961100E+00 3.451970E-01 1308 75 3.661600E-02 2.018600E+00 3.415522E-01 1308 76 3.703580E-02 2.317200E+00 3.291830E-01 1308 77 3.745560E-02 1.658820E+00 3.236762E-01 1308 78 3.787550E-02 1.612700E+00 3.122344E-01 1308 79 3.829530E-02 1.896260E+00 3.196882E-01 1308 80 3.871510E-02 1.310940E+00 3.251420E-01 1308 81 3.913500E-02 1.891500E+00 3.061608E-01 1308 82 3.955480E-02 1.449440E+00 3.126314E-01 1308 83 3.997460E-02 1.782780E+00 3.082108E-01 1308 84 4.039450E-02 1.554700E+00 3.039314E-01 1308 85 4.081430E-02 1.592020E+00 2.911984E-01 1308 86 4.123410E-02 1.725200E+00 2.907320E-01 1308 87 4.165390E-02 1.258280E+00 2.932014E-01 1308 88 4.207380E-02 1.526860E+00 2.954752E-01 1308 89 4.249360E-02 1.485320E+00 2.939664E-01 1308 90 4.291340E-02 1.579880E+00 2.962000E-01 1308 91 4.333330E-02 1.709980E+00 2.885456E-01 1308 92 4.375310E-02 1.346060E+00 2.856168E-01 1308 93 4.417290E-02 1.313240E+00 2.821362E-01 1308 94 4.459280E-02 1.393740E+00 2.706550E-01 1308 95 4.501260E-02 1.194440E+00 2.640830E-01 1308 96 4.543240E-02 1.254900E+00 2.704020E-01 1308 97 4.585220E-02 1.294400E+00 2.805210E-01 1308 98 4.627210E-02 1.260080E+00 2.674192E-01 1308 99 4.669190E-02 1.473460E+00 2.715020E-01 1308 100 4.711170E-02 1.025780E+00 2.630854E-01 1308 101 4.753160E-02 1.207320E+00 2.671144E-01 1308 102 4.795140E-02 1.196900E+00 2.643580E-01 1308 103 4.837120E-02 1.296920E+00 2.514922E-01 1308 104 4.879110E-02 1.109340E+00 2.584286E-01 1308 105 4.921090E-02 9.960200E-01 2.572312E-01 1308 106 4.963070E-02 1.220940E+00 2.540030E-01 1308 107 5.005050E-02 1.061720E+00 2.516096E-01 1308 108 5.047040E-02 1.280980E+00 2.570942E-01 1308 109 5.089020E-02 1.173520E+00 2.469050E-01 1308 110 5.131000E-02 8.240604E-01 2.457388E-01 1308 111 5.172990E-02 1.086840E+00 2.458042E-01 1308 112 5.214970E-02 1.070360E+00 2.449166E-01 1308 113 5.256950E-02 8.493802E-01 2.470668E-01 1308 114 5.298940E-02 9.920398E-01 2.420698E-01 1308 115 5.340920E-02 7.105996E-01 2.394640E-01 1308 116 5.382900E-02 7.768400E-01 2.433822E-01 1308 117 5.424890E-02 9.891798E-01 2.389002E-01 1308 118 5.466870E-02 1.005040E+00 2.407714E-01 1308 119 5.508850E-02 7.437000E-01 2.339984E-01 1308 120 5.550830E-02 9.110202E-01 2.404744E-01 1308 121 5.592820E-02 8.338196E-01 2.383390E-01 1308 122 5.634800E-02 9.162000E-01 2.371594E-01 1308 123 5.676780E-02 9.770400E-01 2.369904E-01 1308 124 5.718770E-02 7.452396E-01 2.311336E-01 1308 125 5.760750E-02 7.003802E-01 2.274372E-01 1308 126 5.802730E-02 4.976400E-01 2.231404E-01 1308 127 5.844720E-02 8.053804E-01 2.274236E-01 1308 128 5.886700E-02 6.490198E-01 2.266434E-01 1308 129 5.928680E-02 8.313798E-01 2.276374E-01 1308 130 5.970660E-02 7.312598E-01 2.316800E-01 1308 131 6.012650E-02 9.752200E-01 2.235646E-01 1308 132 6.054630E-02 7.737602E-01 2.243978E-01 1308 133 6.096610E-02 6.870200E-01 2.219914E-01 1308 134 6.138600E-02 6.312400E-01 2.171140E-01 1308 135 6.180580E-02 7.574200E-01 2.263684E-01 1308 136 6.222560E-02 6.541002E-01 2.166018E-01 1308 137 6.264550E-02 7.442998E-01 2.178380E-01 1308 138 6.306530E-02 9.520400E-01 2.255684E-01 1308 139 6.348510E-02 7.378800E-01 2.167200E-01 1308 140 6.390490E-02 6.442802E-01 2.199266E-01 1308 141 6.432480E-02 7.517400E-01 2.159616E-01 1308 142 6.474460E-02 6.751202E-01 2.148064E-01 1308 143 6.516440E-02 7.175000E-01 2.083632E-01 1308 144 6.558430E-02 6.094400E-01 2.112226E-01 1308 145 6.600410E-02 7.983000E-01 2.134476E-01 1308 146 6.642390E-02 5.033198E-01 2.094950E-01 1308 147 6.684380E-02 5.689800E-01 2.143038E-01 1308 148 6.726360E-02 5.621002E-01 2.081118E-01 1308 149 6.768340E-02 7.853200E-01 2.106076E-01 1308 150 6.810320E-02 7.801406E-01 2.082130E-01 1308 151 6.852310E-02 7.710802E-01 2.070600E-01 1308 152 6.894290E-02 6.554398E-01 2.071294E-01 1308 153 6.936270E-02 5.667604E-01 2.019102E-01 1308 154 6.978260E-02 5.875006E-01 2.056546E-01 1308 155 7.020240E-02 5.541001E-01 2.056698E-01 1308 156 7.062220E-02 6.075202E-01 2.051828E-01 1308 157 7.104210E-02 5.795200E-01 2.025478E-01 1308 158 7.146190E-02 5.204802E-01 2.004912E-01 1308 159 7.188170E-02 6.096590E-01 2.034782E-01 1308 160 7.230160E-02 5.958198E-01 1.961936E-01 1308 161 7.272140E-02 7.058792E-01 2.037230E-01 1308 162 7.314120E-02 5.559000E-01 2.011704E-01 1308 163 7.356100E-02 5.579804E-01 1.972576E-01 1308 164 7.398090E-02 3.996600E-01 1.969284E-01 1308 165 7.440070E-02 4.218408E-01 1.923612E-01 1308 166 7.482050E-02 5.155000E-01 1.956968E-01 1308 167 7.524040E-02 4.960000E-01 1.964608E-01 1308 168 7.566020E-02 5.042392E-01 1.947066E-01 1308 169 7.608000E-02 2.987200E-01 1.942122E-01 1308 170 7.649980E-02 4.825200E-01 1.922196E-01 1308 171 7.691970E-02 3.772000E-01 1.986166E-01 1308 172 7.733950E-02 4.517806E-01 1.961066E-01 1308 173 7.775930E-02 4.494202E-01 1.926818E-01 1308 174 7.817920E-02 5.521600E-01 1.953630E-01 1308 175 7.859900E-02 4.075602E-01 1.899024E-01 1308 176 7.901880E-02 3.166797E-01 1.960762E-01 1308 177 7.943870E-02 6.337800E-01 1.906742E-01 1308 178 7.985850E-02 3.981796E-01 1.928592E-01 1308 179 8.027830E-02 5.411006E-01 1.948158E-01 1308 180 8.069810E-02 4.391398E-01 1.917654E-01 1308 181 8.111800E-02 3.043604E-01 1.933852E-01 1308 182 8.153780E-02 4.722598E-01 1.887790E-01 1308 183 8.195760E-02 4.436600E-01 1.894910E-01 1308 184 8.237750E-02 4.223000E-01 1.907104E-01 1308 185 8.279730E-02 4.195796E-01 1.909328E-01 1308 186 8.321710E-02 3.309803E-01 1.918478E-01 1308 187 8.363700E-02 3.755192E-01 1.892858E-01 1308 188 8.405680E-02 3.188198E-01 1.974828E-01 1308 189 8.447660E-02 3.956400E-01 1.986890E-01 1308 190 8.489650E-02 3.886398E-01 1.992838E-01 1308 191 8.531630E-02 4.389000E-01 2.021372E-01 1308 192 8.573610E-02 3.119604E-01 2.004658E-01 1308 193 8.615590E-02 5.527200E-01 2.083474E-01 1308 194 8.657580E-02 3.095007E-01 2.012960E-01 1308 195 8.699560E-02 4.047206E-01 2.024694E-01 1308 196 8.741540E-02 4.286200E-01 2.013166E-01 1308 197 8.783530E-02 3.155401E-01 2.032890E-01 1308 198 8.825510E-02 4.097798E-01 2.038656E-01 1308 199 8.867490E-02 4.351602E-01 2.067080E-01 1308 200 8.909480E-02 3.478200E-01 2.087260E-01 1308 201 8.951460E-02 2.949397E-01 2.055872E-01 1308 202 8.993440E-02 3.229798E-01 2.079312E-01 1308 203 9.035420E-02 5.049604E-01 2.081894E-01 1308 204 9.077410E-02 2.154398E-01 2.024916E-01 1308 205 9.119390E-02 3.692400E-01 2.092138E-01 1308 206 9.161370E-02 2.138800E-01 2.057134E-01 1308 207 9.203360E-02 3.491400E-01 2.089136E-01 1308 208 9.245340E-02 3.982204E-01 2.082278E-01 1308 209 9.287320E-02 2.615805E-01 2.054048E-01 1308 210 9.329310E-02 1.858198E-01 2.097610E-01 1308 211 9.371290E-02 3.511796E-01 2.118884E-01 1308 212 9.413270E-02 2.595796E-01 2.097728E-01 1308 213 9.455250E-02 2.930197E-01 2.046080E-01 1308 214 9.497240E-02 3.255800E-01 2.100710E-01 1308 215 9.539220E-02 3.706602E-01 2.078858E-01 1308 216 9.581200E-02 2.915600E-01 2.015514E-01 1308 217 9.623190E-02 2.974599E-01 2.072288E-01 1308 218 9.665170E-02 2.229203E-01 2.023676E-01 1308 219 9.707150E-02 1.846997E-01 2.013132E-01 1308 220 9.749140E-02 4.031797E-01 2.029842E-01 1308 221 9.791120E-02 4.014203E-01 2.032100E-01 1308 222 9.833100E-02 3.590400E-01 2.015616E-01 1308 223 9.875080E-02 4.635006E-01 1.956644E-01 1308 224 9.917070E-02 2.886804E-01 1.929386E-01 1308 225 9.959050E-02 2.166002E-01 1.944720E-01 1308 226 1.000100E-01 3.180601E-01 1.949638E-01 1308 227 1.004300E-01 2.562800E-01 2.009582E-01 1308 228 1.008500E-01 4.249000E-01 1.947870E-01 1308 229 1.012700E-01 4.022202E-01 1.992586E-01 1308 230 1.016900E-01 3.206799E-01 1.921446E-01 1308 231 1.021090E-01 2.656800E-01 1.939702E-01 1308 232 1.025290E-01 4.174794E-01 1.959862E-01 1308 233 1.029490E-01 3.453798E-01 1.904256E-01 1308 234 1.033690E-01 3.289200E-01 1.964058E-01 1308 235 1.037890E-01 1.823200E-01 1.917744E-01 1308 236 1.042090E-01 1.840393E-01 1.949852E-01 1308 237 1.046280E-01 2.205800E-01 1.919952E-01 1308 238 1.050480E-01 2.282200E-01 1.897374E-01 1308 239 1.054680E-01 1.906400E-01 1.930058E-01 1308 240 1.058880E-01 3.948602E-01 1.901558E-01 1308 241 1.063080E-01 2.847602E-01 1.896502E-01 1308 242 1.067280E-01 2.915005E-01 1.938544E-01 1308 243 1.071470E-01 2.185002E-01 1.865862E-01 1308 244 1.075670E-01 2.317402E-01 1.909500E-01 1308 245 1.079870E-01 2.790592E-01 1.852438E-01 1308 246 1.084070E-01 2.041606E-01 1.887510E-01 1308 247 1.088270E-01 3.592796E-01 1.895714E-01 1308 248 1.092470E-01 3.381600E-01 1.881994E-01 1308 249 1.096660E-01 2.102198E-01 1.848468E-01 1308 250 1.100860E-01 2.848600E-01 1.887156E-01 1308 251 1.105060E-01 -2.006006E-02 1.848150E-01 1308 252 1.109260E-01 2.300600E-01 1.848100E-01 1308 253 1.113460E-01 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