{
  "code": "SASDWV3",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "The <i>Paulinella</i>\n              chromatophore transit peptide part2 adopts a structural fold similar to the γ-glutamyl-cyclotransferase fold",
    "publication": {
      "title": "The <i>Paulinella</i>\n              chromatophore transit peptide part2 adopts a structural fold similar to the γ-glutamyl-cyclotransferase fold",
      "author_list": "Klimenko V, Reiners J, Applegate V, Reimann K, Popowicz G, Hoeppner A, Papadopoulos A, Smits S, Nowack E",
      "journal": "Plant Physiology",
      "doi": "10.1093/plphys/kiaf504",
      "pmid": null,
      "published_date": "2025 Oct 10"
    },
    "status": "released",
    "submitted_date": "2024-12-04",
    "released_date": "2025-10-13"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDWV3.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDWV3.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWV3_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWV3_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWV3_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWV3_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDWV3.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 6M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "crTPpart2 of RNA helicase (RnaH)",
          "short_name": "crTPpart2_RnaH",
          "sequence": "GMLDFSSPAPRKPFSRNSRTTVREGPPPAAKPFWPSDDLEDVVVKPGAPVAGLALLAPDDTTGCLFTYASLKEHDEQDEEFAEGSTVEDAWLYGAKLNHNGPFAYATGNTTDIVKGRVLCWPAETFEDKLEEVYIFRKFDPDQPQEGSIRCSIAPVVLRDGSSTDAVWFHQTPESIVPKQKKKTNTPKIAESF",
          "organism": "Paulinella chromatophora",
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "",
          "mw": 21.288,
          "total_mw": 21.288,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "No UniProt ID is available. This sequence was derived from the transcripts (GenBank: GEZN00000000.1,  Nowack, E. C. M., Price, D. C., Bhattacharya, D., Singer, A., Melkonian, M. and Grossman, A. R. (2016) Gene transfers from diverse bacteria compensate for reductive genome evolution in the chromatophore of Paulinella chromatophora. Proc. Nati. Acad. Sci. USA, 113, 12214-12219.)  Scaffold2581-size2436, encoding a protein annotated as RNA helicase (RnaH),"
        }
      ],
      "buffer": {
        "name": "20 mM HEPES, 300 mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "crTPpart2 of RNA helicase (RnaH)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Düsseldorf, Germany",
            "full_name": "Heinrich Heine University Düsseldorf",
            "webpage": "https://www.uni-duesseldorf.de"
          }
        ],
        "contributor_name": "Jens",
        "contributor_surname": "Reiners",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2021-06-15",
    "storage_temperature": 10.0,
    "cell_temperature": 10.0,
    "exposure_time": 0.095,
    "number_of_frames": 40,
    "wavelength": 0.124,
    "sample_detector_distance": 3.0,
    "concentration_min": 1.16,
    "concentration_max": 9.3,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.077,
    "s_max": 6.983,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDWV3_fit1_model1_img.png",
          "software": "EOM/RANCH",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "mix",
          "software_version": "GAJOE - version 2.1 - (r14885)",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDWV3_fit1_model1.pdb",
          "model_mw": 21.5,
          "bead_radius": 1.9,
          "log": "https://www.sasbdb.org/media/log_files/logFile_001_1_XWRGKiY.log",
          "symmetry": "P1",
          "comment": "Volume fraction (2/9)",
          "user": 402
        },
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDWV3_fit1_model2_img.png",
          "software": "EOM/RANCH",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "mix",
          "software_version": "GAJOE - version 2.1 - (r14885)",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDWV3_fit1_model2.pdb",
          "model_mw": 21.5,
          "bead_radius": 1.9,
          "log": "https://www.sasbdb.org/media/log_files/logFile_001_1_e9dmc5Z.log",
          "symmetry": "P1",
          "comment": "Volume fraction (5/9)",
          "user": 402
        },
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDWV3_fit1_model3_img.png",
          "software": "EOM/RANCH",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "mix",
          "software_version": "GAJOE - version 2.1 - (r14885)",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDWV3_fit1_model3.pdb",
          "model_mw": 21.5,
          "bead_radius": 1.9,
          "log": "https://www.sasbdb.org/media/log_files/logFile_001_1_3bfCnmC.log",
          "symmetry": "P1",
          "comment": "Volume fraction (1/9)",
          "user": 402
        },
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDWV3_fit1_model4_img.png",
          "software": "EOM/RANCH",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "mix",
          "software_version": "GAJOE - version 2.1 - (r14885)",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDWV3_fit1_model4.pdb",
          "model_mw": 21.5,
          "bead_radius": 1.9,
          "log": "https://www.sasbdb.org/media/log_files/logFile_001_1_sJJXUi8.log",
          "symmetry": "P1",
          "comment": "Volume fraction (1/9)",
          "user": 402
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDWV3_fit1_fixed_fit_img.png",
      "software": "EOM/GAJOE",
      "chi_square_value": 1.062,
      "p_value": 0.0,
      "fit_residual_plot": "SASDWV3_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDWV3_fit1.fit",
      "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/logFile_001_1_52dhJQd.log",
      "software_version": "GAJOE - version 2.1 - (r14885)",
      "description": ""
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of crTPpart2 of RnaH in 20 mM HEPES, 300 mM NaCl, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.2 and 9.3 mg/ml were measured at 10°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.",
  "tags": [],
  "intensity_unit": "1/cm",
  "experimental_mw": 21.18,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 28.0,
  "porod_mw_error": null,
  "pddf_i0": 0.01658,
  "pddf_i0_error": null,
  "guinier_i0": 0.0163714,
  "guinier_i0_error": null,
  "pddf_rg": 2.482,
  "pddf_rg_error": null,
  "guinier_rg": 2.34,
  "guinier_rg_error": 0.011,
  "pddf_dmax": 9.17,
  "pddf_dmax_error": null,
  "porod_volume": 45.61,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 4,
  "guinier_point_last": 171,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDWV3_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2025-10-13T13:58:54.876094+02:00",
  "bragg_peak": []
}