{ "code": "SASDEV5", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "Solution structures of long-acting insulin analogues and their complexes with albumin.", "publication": { "title": "Solution structures of long-acting insulin analogues and their complexes with albumin.", "author_list": "Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P", "journal": "Acta Crystallogr D Struct Biol", "doi": "10.1107/S2059798318017552", "pmid": "30950398", "published_date": "2019 Mar 1" }, "status": "released", "submitted_date": "2018-10-03", "released_date": "2019-04-07" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDEV5.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDEV5.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV5_dat_img.png", "intensities_kratky_plot": 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"GIVEQCCTSICSLYQLENYCN\r\nFVNQHLCGSHLVEALYLVCGERGFFYTPK", "organism": null, "uniprot_code": "P01308", "uniprot_range_first": 25, "uniprot_range_last": 110, "oligomerization": "18-mer", "molecular_type": "protein", "uniprot_sequence": "MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAED\nLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN", "mw": 5.9, "total_mw": 106.2, "number_molecules": 18, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "5.0 mM Na2HPO4, 13.1 mM m-cresol, 15.1 mM phenol, 173.7 mM glycerol, 20.0 mM NaCl", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.4, "deuteration": null }, "purity_method": null, "name": "Insulin detemir tri-hexamer", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Lyngby, Denmark", "full_name": "Technical University of Denmark", "webpage": "http://www.dtu.dk" } ], "contributor_name": "Line Abildgaard", "contributor_surname": "Ryberg", "orcid": "" } ], "concentration_method": null, "concentration_unit": null, "date": "2015-09-25", "storage_temperature": null, "cell_temperature": 20.0, "exposure_time": 30.0, "number_of_frames": 4, "wavelength": 0.091, "sample_detector_distance": 1.962, "concentration_min": null, "concentration_max": 2.5, "sample_volume": null, "flow_rate": null, "s_min": 0.085, "s_max": 5.183, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDEV5_fit2_model1_img.png", "software": "SASREF CV", "pdb_link": [ { "pdb_code": "1trz", "ext_code": "1TRZ", "ext_type": "pdb", "difference_with_model": "derived" } ], "model_title": null, "type_of_model": "atomic", "software_version": null, "model_data": 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"https://www.sasbdb.org/media/fitting_files/scattering_plots/dammif_fit_img.png", "software": "DAMMIF", "chi_square_value": 0.802, "p_value": 6e-06, "fit_residual_plot": "dammif_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDEV5_fit3.fir", "fit_log": null, "software_version": null, "description": "" } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of insulin detemir tri-hexamer in 5.0 mM Na2HPO4, 13.1 mM m-cresol, 15.1 mM phenol, 173.7 mM glycerol, 20.0 mM NaCl, pH 7.4 were collected on the I911-4 beam line at the MAX IV storage ring (Lund, Sweden) using a Dectris Pilatus 1M detector at a sample-detector distance of 2.0 m and at a wavelength of λ = 0.091 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.50 mg/ml was measured at 20°C. Four successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.", "tags": [], "intensity_unit": "1/cm", "experimental_mw": 100.3, "experimental_mw_error": null, "guinier_i0_mw": 100.3, "guinier_i0_mw_error": null, "porod_mw": 91.5, "porod_mw_error": null, "pddf_i0": 0.07362, "pddf_i0_error": null, "guinier_i0": 0.072, "guinier_i0_error": null, "pddf_rg": 3.35, "pddf_rg_error": null, "guinier_rg": 3.26, "guinier_rg_error": null, "pddf_dmax": 11.32, "pddf_dmax_error": null, "porod_volume": 137.2, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": null, "guinier_point_last": null, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDEV5_datloglog_img.png", "symmetry": null, "last_modified": "2020-11-27T11:56:59.741576+01:00", "bragg_peak": [] }