{ "code": "SASDEV7", "status": "Published", "type_of_curve": "Merged", "angular_unit": "1/A", "project": { "title": "The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility.", "publication": { "title": "The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility.", "author_list": "Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV", "journal": "Nat Microbiol", "doi": "10.1038/s41564-019-0622-3", "pmid": "31844299", "published_date": "2019 Dec 16" }, "status": "released", "submitted_date": "2018-11-12", "released_date": "2019-11-14" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDEV7.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDEV7.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV7_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV7_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEV7_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV7_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDEV7.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus3 X 2M", "resolution": 172.0 }, "name": "Advanced Light Source (ALS)", "city": "Berkeley, CA", "country": "USA", "beamline_name": "12.3.1 (SIBYLS)", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Stator protein FlaG soluble domain", "short_name": "sFlaG", "sequence": "MGSSHHHHHHSQDPNSISTTMSSYSIQQSQKMLTQLQIDYATNTSSNTVVAYLHNVGETTISYLQNSVVYFGPNGQLQPVGYNSGSSPYWTVTSNSLQPGSVVKIIIYLSSPLSSNQYYTIQIVTPNGYTVSYMF", "organism": "Sulfolobus acidocaldarius", "uniprot_code": "Q4J9K7", "uniprot_range_first": 32, "uniprot_range_last": 151, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MVSEVISETIMIIVSVTLIGVLAGTVFSVASSISTTMSSYSIQQSQKMLTQLQIDYATNT\nSSNTVVAYLHNVGETTISYLQNSVVYFGPNGQLQPVGYNSGSSPYWTVTSNSLQPGSVVK\nIIIYLSSPLSSNQYYTIQIVTPNGYTVSYMFKVS", "mw": 14.932, "total_mw": 29.864, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null }, { "long_name": "Conserved flagellar protein FlaF-I96Y soluble domain", "short_name": "sFlaF-I96Y", "sequence": "MGSSHHHHHHSQDPNSNQAQELNHELELEQLETKITVSSVSLTGSTLNVVLENNGSTNLYDFQGFSVIVQYYANISNYSTFNLSLYNYTKNSNPSPYYWTINTPLLAPGSQATLTIILPYPPYPNTQATVVIVTNYGPSVIWRGSL", "organism": "Sulfolobus acidocaldarius", "uniprot_code": "Q4J9K8", "uniprot_range_first": 35, "uniprot_range_last": 164, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MGVSQTLAFILITFIGVSFGIVLLSAYFKSQQILNQAQELNHELELEQLETKITVSSVSL\nTGSTLNVVLENNGSTNLYDFQGFSVIVQYYANISNISTFNLSLYNYTKNSNPSPYYWTIN\nTPLLAPGSQATLTIILPYPPYPNTQATVVIVTNYGPSVIWRGSL", "mw": 16.289, "total_mw": 32.578, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 3.0, "deuteration": null }, "purity_method": null, "name": "Stator protein complex FlaG/FlaF-I96Y", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Texas, USA", "full_name": "MD Anderson Cancer Center", "webpage": "https://www.mdanderson.org/" } ], "contributor_name": "Chi-Lin", "contributor_surname": "Tsai", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2016-11-10", "storage_temperature": null, "cell_temperature": 10.0, "exposure_time": 0.3, "number_of_frames": 33, "wavelength": 0.127, "sample_detector_distance": 1.5, "concentration_min": 1.5, "concentration_max": 3.0, "sample_volume": null, "flow_rate": null, "s_min": 0.125, "s_max": 3.698, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDEV7_fit1_model1_img.png", "software": "BILBOMD", "pdb_link": [ { "pdb_code": "5tug", "ext_code": "5TUG", "ext_type": "pdb", "difference_with_model": "derived" } ], "model_title": null, "type_of_model": "mix", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDEV7_fit1_model1.pdb", "model_mw": 187.9, "bead_radius": null, "log": null, "symmetry": null, "comment": "The His-tag is modeled by Modeller", "user": 321 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEV7_fit1_fixed_fit_img.png", "software": "FoXS", "chi_square_value": 10.038432, "p_value": 0.0, "fit_residual_plot": "SASDEV7_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDEV7_fit1.fit", "fit_log": null, "software_version": null, "description": "" } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "4.5a", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of the stator protein complex FlaG/FlaF-I96Y in 25 mM citric acid/sodium citrate, 150mM NaCl, 3% glycerol, pH 3 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.5 and 3 mg/ml were measured at 10°C. 33 successive 0.300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.", "tags": [], "intensity_unit": "1/cm", "experimental_mw": 50.85, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 56.0, "porod_mw_error": null, "pddf_i0": 208.5, "pddf_i0_error": null, "guinier_i0": 208.48, "guinier_i0_error": 0.22, "pddf_rg": 2.67, "pddf_rg_error": null, "guinier_rg": 2.675, "guinier_rg_error": 0.004, "pddf_dmax": 8.2, "pddf_dmax_error": null, "porod_volume": 90.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 62, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDEV7_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-19T18:54:58.540247+01:00", "bragg_peak": [] }