data_SASDFW2_MAIN # _sas_result.id 1309 _sas_result.SASBDB_code SASDFW2 _sas_result.experimental_MW 29.920 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 30.9700000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 13.080 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 1 _sas_result.Guinier_point_max 10 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73A) polymer frame no. 300-304 ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 300-304)" _sas_scan.detc_id 15 _sas_scan.id 1309 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1270 _sas_scan.result_id 1309 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1270 _sas_sample.name "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 300-304)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1270 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 2.924020E+01 1.463365E+01 1309 2 5.968380E-03 2.641600E+01 8.274774E+00 1309 3 6.388220E-03 2.367700E+01 6.057508E+00 1309 4 6.808050E-03 2.226480E+01 4.185686E+00 1309 5 7.227880E-03 2.252440E+01 3.665626E+00 1309 6 7.647710E-03 2.117020E+01 3.058194E+00 1309 7 8.067540E-03 2.228250E+01 2.355354E+00 1309 8 8.487370E-03 2.194820E+01 2.516332E+00 1309 9 8.907200E-03 1.905364E+01 2.187800E+00 1309 10 9.327030E-03 1.845160E+01 1.926012E+00 1309 11 9.746860E-03 1.640506E+01 1.682558E+00 1309 12 1.016670E-02 1.609898E+01 1.647104E+00 1309 13 1.058650E-02 1.637102E+01 1.415374E+00 1309 14 1.100630E-02 1.491166E+01 1.383202E+00 1309 15 1.142620E-02 1.508714E+01 1.354972E+00 1309 16 1.184600E-02 1.391780E+01 1.202320E+00 1309 17 1.226580E-02 1.448324E+01 1.209050E+00 1309 18 1.268570E-02 1.473166E+01 1.092354E+00 1309 19 1.310550E-02 1.245612E+01 1.108608E+00 1309 20 1.352530E-02 1.258908E+01 1.022003E+00 1309 21 1.394520E-02 1.185770E+01 9.920310E-01 1309 22 1.436500E-02 1.093532E+01 9.305604E-01 1309 23 1.478480E-02 1.164420E+01 8.820878E-01 1309 24 1.520460E-02 1.078230E+01 8.863476E-01 1309 25 1.562450E-02 1.012068E+01 7.723054E-01 1309 26 1.604430E-02 1.026328E+01 7.788884E-01 1309 27 1.646410E-02 9.276860E+00 7.216584E-01 1309 28 1.688400E-02 9.119180E+00 6.769844E-01 1309 29 1.730380E-02 9.091620E+00 6.642094E-01 1309 30 1.772360E-02 8.707660E+00 6.457740E-01 1309 31 1.814350E-02 7.809100E+00 5.948694E-01 1309 32 1.856330E-02 7.927900E+00 5.997396E-01 1309 33 1.898310E-02 7.109140E+00 5.882414E-01 1309 34 1.940290E-02 7.709040E+00 5.747964E-01 1309 35 1.982280E-02 7.212780E+00 5.259440E-01 1309 36 2.024260E-02 6.623040E+00 5.558838E-01 1309 37 2.066240E-02 6.463420E+00 5.274858E-01 1309 38 2.108230E-02 6.406220E+00 5.283614E-01 1309 39 2.150210E-02 5.969840E+00 5.076206E-01 1309 40 2.192190E-02 6.054940E+00 4.826732E-01 1309 41 2.234180E-02 5.376080E+00 4.838628E-01 1309 42 2.276160E-02 5.233420E+00 4.753678E-01 1309 43 2.318140E-02 5.333540E+00 4.612748E-01 1309 44 2.360130E-02 5.478500E+00 4.627504E-01 1309 45 2.402110E-02 4.504220E+00 4.518000E-01 1309 46 2.444090E-02 4.849660E+00 4.304982E-01 1309 47 2.486070E-02 4.451100E+00 4.157524E-01 1309 48 2.528060E-02 4.611200E+00 4.197238E-01 1309 49 2.570040E-02 3.983540E+00 4.068504E-01 1309 50 2.612020E-02 4.069300E+00 3.945258E-01 1309 51 2.654010E-02 3.769540E+00 4.029732E-01 1309 52 2.695990E-02 3.471620E+00 3.786628E-01 1309 53 2.737970E-02 3.358820E+00 3.933950E-01 1309 54 2.779960E-02 3.254480E+00 3.767228E-01 1309 55 2.821940E-02 3.291100E+00 3.932226E-01 1309 56 2.863920E-02 3.086780E+00 3.939624E-01 1309 57 2.905900E-02 3.181140E+00 3.770958E-01 1309 58 2.947890E-02 2.956020E+00 3.858692E-01 1309 59 2.989870E-02 3.182660E+00 3.707908E-01 1309 60 3.031850E-02 3.189680E+00 3.778402E-01 1309 61 3.073840E-02 2.983400E+00 3.719876E-01 1309 62 3.115820E-02 2.793380E+00 3.740676E-01 1309 63 3.157800E-02 2.551620E+00 3.808802E-01 1309 64 3.199790E-02 2.595460E+00 3.701742E-01 1309 65 3.241770E-02 2.459980E+00 3.673364E-01 1309 66 3.283750E-02 2.219000E+00 3.558072E-01 1309 67 3.325730E-02 2.421900E+00 3.588382E-01 1309 68 3.367720E-02 2.471660E+00 3.581048E-01 1309 69 3.409700E-02 2.004880E+00 3.604776E-01 1309 70 3.451680E-02 2.241620E+00 3.565452E-01 1309 71 3.493670E-02 1.845840E+00 3.387974E-01 1309 72 3.535650E-02 2.107820E+00 3.461084E-01 1309 73 3.577630E-02 2.472540E+00 3.465856E-01 1309 74 3.619620E-02 2.270020E+00 3.423154E-01 1309 75 3.661600E-02 1.968120E+00 3.398212E-01 1309 76 3.703580E-02 2.025400E+00 3.444788E-01 1309 77 3.745560E-02 1.609360E+00 3.345194E-01 1309 78 3.787550E-02 1.984640E+00 3.322892E-01 1309 79 3.829530E-02 1.761840E+00 3.160472E-01 1309 80 3.871510E-02 1.833320E+00 3.276020E-01 1309 81 3.913500E-02 1.561140E+00 3.060362E-01 1309 82 3.955480E-02 1.672440E+00 3.158912E-01 1309 83 3.997460E-02 1.610340E+00 3.077572E-01 1309 84 4.039450E-02 1.580440E+00 3.016720E-01 1309 85 4.081430E-02 1.664220E+00 3.109896E-01 1309 86 4.123410E-02 1.652220E+00 2.970118E-01 1309 87 4.165390E-02 1.630520E+00 2.975544E-01 1309 88 4.207380E-02 1.604880E+00 2.903726E-01 1309 89 4.249360E-02 1.621780E+00 2.900712E-01 1309 90 4.291340E-02 1.385980E+00 2.822226E-01 1309 91 4.333330E-02 1.368040E+00 2.841722E-01 1309 92 4.375310E-02 1.385500E+00 2.952184E-01 1309 93 4.417290E-02 1.290860E+00 2.739898E-01 1309 94 4.459280E-02 1.419340E+00 2.724180E-01 1309 95 4.501260E-02 1.232140E+00 2.652726E-01 1309 96 4.543240E-02 1.324260E+00 2.760730E-01 1309 97 4.585220E-02 1.248880E+00 2.702438E-01 1309 98 4.627210E-02 1.282200E+00 2.672200E-01 1309 99 4.669190E-02 1.132700E+00 2.655482E-01 1309 100 4.711170E-02 1.297800E+00 2.668084E-01 1309 101 4.753160E-02 1.114220E+00 2.627684E-01 1309 102 4.795140E-02 9.620998E-01 2.626540E-01 1309 103 4.837120E-02 9.683798E-01 2.515214E-01 1309 104 4.879110E-02 9.122800E-01 2.549786E-01 1309 105 4.921090E-02 9.826200E-01 2.569384E-01 1309 106 4.963070E-02 1.087740E+00 2.630410E-01 1309 107 5.005050E-02 1.018820E+00 2.527104E-01 1309 108 5.047040E-02 1.068140E+00 2.518516E-01 1309 109 5.089020E-02 1.104480E+00 2.511906E-01 1309 110 5.131000E-02 8.814000E-01 2.474480E-01 1309 111 5.172990E-02 8.371000E-01 2.525210E-01 1309 112 5.214970E-02 9.566200E-01 2.450154E-01 1309 113 5.256950E-02 7.458002E-01 2.468336E-01 1309 114 5.298940E-02 8.768798E-01 2.468718E-01 1309 115 5.340920E-02 1.108220E+00 2.438098E-01 1309 116 5.382900E-02 9.480998E-01 2.455970E-01 1309 117 5.424890E-02 9.943198E-01 2.383922E-01 1309 118 5.466870E-02 9.503000E-01 2.443224E-01 1309 119 5.508850E-02 1.061200E+00 2.400198E-01 1309 120 5.550830E-02 9.631600E-01 2.396914E-01 1309 121 5.592820E-02 8.908598E-01 2.375988E-01 1309 122 5.634800E-02 6.398604E-01 2.253580E-01 1309 123 5.676780E-02 9.672600E-01 2.408786E-01 1309 124 5.718770E-02 7.050800E-01 2.288806E-01 1309 125 5.760750E-02 6.480404E-01 2.323254E-01 1309 126 5.802730E-02 7.036198E-01 2.266066E-01 1309 127 5.844720E-02 6.868604E-01 2.260424E-01 1309 128 5.886700E-02 6.913596E-01 2.282834E-01 1309 129 5.928680E-02 7.250998E-01 2.297198E-01 1309 130 5.970660E-02 5.406400E-01 2.292376E-01 1309 131 6.012650E-02 6.259198E-01 2.259998E-01 1309 132 6.054630E-02 7.974002E-01 2.300256E-01 1309 133 6.096610E-02 6.078000E-01 2.247964E-01 1309 134 6.138600E-02 4.973004E-01 2.200872E-01 1309 135 6.180580E-02 6.123202E-01 2.242758E-01 1309 136 6.222560E-02 6.422000E-01 2.210736E-01 1309 137 6.264550E-02 7.703796E-01 2.203738E-01 1309 138 6.306530E-02 8.377992E-01 2.190360E-01 1309 139 6.348510E-02 6.671798E-01 2.142394E-01 1309 140 6.390490E-02 4.626602E-01 2.201566E-01 1309 141 6.432480E-02 5.494400E-01 2.140980E-01 1309 142 6.474460E-02 6.609600E-01 2.149338E-01 1309 143 6.516440E-02 5.113600E-01 2.078882E-01 1309 144 6.558430E-02 8.077200E-01 2.128082E-01 1309 145 6.600410E-02 6.327194E-01 2.164786E-01 1309 146 6.642390E-02 3.667598E-01 2.090474E-01 1309 147 6.684380E-02 5.438600E-01 2.108124E-01 1309 148 6.726360E-02 6.354402E-01 2.116944E-01 1309 149 6.768340E-02 5.047402E-01 2.107586E-01 1309 150 6.810320E-02 5.305806E-01 2.059514E-01 1309 151 6.852310E-02 6.394002E-01 2.104252E-01 1309 152 6.894290E-02 6.919398E-01 2.046952E-01 1309 153 6.936270E-02 5.286804E-01 2.072868E-01 1309 154 6.978260E-02 4.141606E-01 2.066484E-01 1309 155 7.020240E-02 5.726804E-01 2.057432E-01 1309 156 7.062220E-02 6.337002E-01 2.046454E-01 1309 157 7.104210E-02 5.407600E-01 2.052896E-01 1309 158 7.146190E-02 4.642600E-01 1.985712E-01 1309 159 7.188170E-02 5.401196E-01 2.001084E-01 1309 160 7.230160E-02 4.127600E-01 2.004964E-01 1309 161 7.272140E-02 6.212398E-01 2.029144E-01 1309 162 7.314120E-02 5.710200E-01 1.992664E-01 1309 163 7.356100E-02 4.102608E-01 1.963998E-01 1309 164 7.398090E-02 6.209200E-01 2.004208E-01 1309 165 7.440070E-02 4.617802E-01 1.964294E-01 1309 166 7.482050E-02 3.563806E-01 1.988970E-01 1309 167 7.524040E-02 4.379000E-01 1.935792E-01 1309 168 7.566020E-02 5.978992E-01 1.952664E-01 1309 169 7.608000E-02 2.662800E-01 1.949858E-01 1309 170 7.649980E-02 3.498200E-01 1.950286E-01 1309 171 7.691970E-02 3.965600E-01 1.921740E-01 1309 172 7.733950E-02 4.088404E-01 1.943970E-01 1309 173 7.775930E-02 3.958200E-01 1.894508E-01 1309 174 7.817920E-02 4.476400E-01 1.933948E-01 1309 175 7.859900E-02 5.894800E-01 1.909962E-01 1309 176 7.901880E-02 3.086197E-01 1.923130E-01 1309 177 7.943870E-02 4.162400E-01 1.890332E-01 1309 178 7.985850E-02 4.242396E-01 1.939070E-01 1309 179 8.027830E-02 4.868200E-01 1.904880E-01 1309 180 8.069810E-02 4.163596E-01 1.913318E-01 1309 181 8.111800E-02 5.671208E-01 1.948548E-01 1309 182 8.153780E-02 2.422995E-01 1.871020E-01 1309 183 8.195760E-02 5.072200E-01 1.916462E-01 1309 184 8.237750E-02 2.444997E-01 1.892862E-01 1309 185 8.279730E-02 2.139196E-01 1.882442E-01 1309 186 8.321710E-02 1.808202E-01 1.863512E-01 1309 187 8.363700E-02 3.711798E-01 1.911944E-01 1309 188 8.405680E-02 3.405600E-01 1.996180E-01 1309 189 8.447660E-02 3.381600E-01 1.992820E-01 1309 190 8.489650E-02 4.723995E-01 2.002504E-01 1309 191 8.531630E-02 2.502200E-01 2.028650E-01 1309 192 8.573610E-02 3.646602E-01 2.026300E-01 1309 193 8.615590E-02 2.469199E-01 2.023502E-01 1309 194 8.657580E-02 4.978406E-01 2.032640E-01 1309 195 8.699560E-02 4.376600E-01 2.047208E-01 1309 196 8.741540E-02 2.445601E-01 2.036868E-01 1309 197 8.783530E-02 4.293200E-01 2.054698E-01 1309 198 8.825510E-02 3.055998E-01 2.051644E-01 1309 199 8.867490E-02 3.971202E-01 2.102580E-01 1309 200 8.909480E-02 3.956398E-01 2.091870E-01 1309 201 8.951460E-02 3.709798E-01 2.044234E-01 1309 202 8.993440E-02 4.209198E-01 2.123178E-01 1309 203 9.035420E-02 2.290402E-01 2.059826E-01 1309 204 9.077410E-02 3.108600E-01 2.038844E-01 1309 205 9.119390E-02 2.264200E-01 2.039530E-01 1309 206 9.161370E-02 5.218799E-01 2.096174E-01 1309 207 9.203360E-02 2.786000E-01 2.081866E-01 1309 208 9.245340E-02 3.978800E-01 2.026248E-01 1309 209 9.287320E-02 4.583206E-01 2.062176E-01 1309 210 9.329310E-02 4.250396E-01 2.119460E-01 1309 211 9.371290E-02 2.662994E-01 2.121532E-01 1309 212 9.413270E-02 2.843994E-01 2.126196E-01 1309 213 9.455250E-02 3.577200E-01 2.081042E-01 1309 214 9.497240E-02 3.428406E-01 2.073546E-01 1309 215 9.539220E-02 3.543600E-01 2.056044E-01 1309 216 9.581200E-02 2.419999E-01 2.034276E-01 1309 217 9.623190E-02 2.154799E-01 2.042018E-01 1309 218 9.665170E-02 3.424000E-01 2.022102E-01 1309 219 9.707150E-02 2.608193E-01 2.044262E-01 1309 220 9.749140E-02 2.299398E-01 2.045378E-01 1309 221 9.791120E-02 3.467800E-01 2.024156E-01 1309 222 9.833100E-02 2.413601E-01 2.005096E-01 1309 223 9.875080E-02 3.736203E-01 1.984666E-01 1309 224 9.917070E-02 2.357802E-01 1.957136E-01 1309 225 9.959050E-02 2.530600E-01 1.926854E-01 1309 226 1.000100E-01 2.778400E-01 1.933616E-01 1309 227 1.004300E-01 1.926999E-01 1.982554E-01 1309 228 1.008500E-01 3.831998E-01 1.936302E-01 1309 229 1.012700E-01 2.222802E-01 1.976550E-01 1309 230 1.016900E-01 1.366599E-01 1.912668E-01 1309 231 1.021090E-01 1.146802E-01 1.897066E-01 1309 232 1.025290E-01 1.486996E-01 1.956166E-01 1309 233 1.029490E-01 3.271396E-01 1.979854E-01 1309 234 1.033690E-01 1.995203E-01 1.938460E-01 1309 235 1.037890E-01 1.553397E-01 1.898104E-01 1309 236 1.042090E-01 2.414792E-01 1.902004E-01 1309 237 1.046280E-01 2.654203E-01 1.927162E-01 1309 238 1.050480E-01 1.336601E-01 1.894600E-01 1309 239 1.054680E-01 1.152801E-01 1.926562E-01 1309 240 1.058880E-01 1.576202E-01 1.897746E-01 1309 241 1.063080E-01 2.124804E-01 1.856972E-01 1309 242 1.067280E-01 2.383603E-01 1.892946E-01 1309 243 1.071470E-01 2.532404E-01 1.903000E-01 1309 244 1.075670E-01 1.716003E-01 1.906104E-01 1309 245 1.079870E-01 2.788594E-01 1.889942E-01 1309 246 1.084070E-01 1.143607E-01 1.882888E-01 1309 247 1.088270E-01 1.929995E-01 1.866488E-01 1309 248 1.092470E-01 1.364001E-01 1.859244E-01 1309 249 1.096660E-01 2.755594E-01 1.878352E-01 1309 250 1.100860E-01 3.263198E-01 1.862160E-01 1309 251 1.105060E-01 3.503397E-01 1.899586E-01 1309 252 1.109260E-01 1.342200E-01 1.834210E-01 1309 253 1.113460E-01 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