data_SASDEW4_MAIN # _sas_result.id 1117 _sas_result.SASBDB_code SASDEW4 _sas_result.experimental_MW 334.000 _sas_result.experimental_MW_error 30.000 _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod 503.000 _sas_result.MW_Porod_error 50.000 _sas_result.I0_from_PR 0.1290000000 _sas_result.I0_from_PR_error 0.0010000000 _sas_result.I0_from_Guinier 0.1290000000 _sas_result.I0_from_Guinier_error 0.0010000000 _sas_result.Rg_from_PR 6.000 _sas_result.Rg_from_PR_error 0.010 _sas_result.Rg_from_Guinier 5.880 _sas_result.Rg_from_Guinier_error 0.010 _sas_result.D_max 22.00 _sas_result.Porod_volume 805.00 _sas_result.Porod_volume_error 80.00 _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 22 _sas_result.Guinier_point_max 72 _sas_result.type_of_curve single_conc _sas_result.standard . _sas_result.Dmax_error 0.50 _sas_result.comments 'ACA8 protein complex with Calmodulin encapsulated in a partially deuterated "stealth-nanodisc": (sND). Disc components (MSP1D1 scaffold protein - 75% deuterated, and POPC lipid - heads/tails 78% and 92% deuterated).' # _sas_scan.title "ACA8 + d75-Cam7 in stealth nanodisc" _sas_scan.detc_id 4 _sas_scan.id 1117 _sas_scan.measurement_date 2017-09-08 _sas_scan.storage_temperature 10.0 _sas_scan.cell_temperature 10.0 _sas_scan.exposure_time 0.050 _sas_scan.number_of_frames 20 _sas_scan.unit 1/nm _sas_scan.qmin 0.0249 _sas_scan.qmax 5.0640 _sas_scan.sample_id 1077 _sas_scan.result_id 1117 _sas_scan.beam_id 6 # _sas_beam.id 6 _sas_beam.instrument_name "PETRA III EMBL P12" _sas_beam.instrument_city Hamburg _sas_beam.instrument_country Germany _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.124000 _sas_beam.dist_spec_to_detc 3.30000 # _sas_detc.id 4 _sas_detc.name "Pilatus 2M" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1077 _sas_sample.name "ACA8 complex with Calmodulin (hydrogenated) in stealth nanodisc" _sas_sample.ext_coefficient . _sas_sample.contrast 1.876 _sas_sample.specific_vol 0.8100 _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 1.00-6.00 _sas_sample.concentration_method A280 loop_ _sas_sample.entity_id 1017 1018 1312 1314 # _sas_buffer.id 691 _sas_buffer.name "30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1077 # loop_ _entity.id _entity.type _entity.pdbx_description _entity.formula_weight _entity.pdbx_number_of_molecules 1017 protein "Membrane scaffold protein 1D1 (deuterated, 75%)" 24.661 2 1018 other "1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)" 0.760 . 1312 protein "Calcium-transporting ATPase 8, plasma membrane-type" 118.209 1 1314 protein Calmodulin-7 . 1 # loop_ _entity_name_com.entity_id _entity_name_com.name 1017 d75-msp1d1 1018 d(78,92)-POPC 1312 ACA8 1314 Cam7 # loop_ _entity_poly.entity_id _entity_poly.pdbx_seq_one_letter_code 1017 ;GHHHHHHHDYDIPTTENLYFQGSTFSKLREQLGPVTQEFWDNLEKETEGLRQEM SKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQE KLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEY HAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ ; 1018 C42H82NO8P 1312 ;HHHHHHHHENLYFQGS MTSLLKSSPGRRRGGDVESGKSEHADSDSDTFYIPSKNASIERLQQWRKAALVLNASRRF RYTLDLKKEQETREMRQKIRSHAHALLAANRFMDMGRESGVEKTTGPATPAGDFGITPEQ LVIMSKDHNSGALEQYGGTQGLANLLKTNPEKGISGDDDDLLKRKTIYGSNTYPRKKGKG FLRFLWDACHDLTLIILMVAAVASLALGIKTEGIKEGWYDGGSIAFAVILVIVVTAVSDY KQSLQFQNLNDEKRNIHLEVLRGGRRVEISIYDIVVGDVIPLNIGNQVPADGVLISGHSL ALDESSMTGESKIVNKDANKDPFLMSGCKVADGNGSMLVTGVGVNTEWGLLMASISEDNG EETPLQVRLNGVATFIGSIGLAVAAAVLVILLTRYFTGHTKDNNGGPQFVKGKTKVGHVI DDVVKVLTVAVTIVVVAVPEGLPLAVTLTLAYSMRKMMADKALVRRLSACETMGSATTIC SDKTGTLTLNQMTVVESYAGGKKTDTEQLPATITSLVVEGISQNTTGSIFVPEGGGDLEY SGSPTEKAILGWGVKLGMNFETARSQSSILHAFPFNSEKKRGGVAVKTADGEVHVHWKGA SEIVLASCRSYIDEDGNVAPMTDDKASFFKNGINDMAGRTLRCVALAFRTYEAEKVPTGE ELSKWVLPEDDLILLAIVGIKDPCRPGVKDSVVLCQNAGVKVRMVTGDNVQTARAIALEC GILSSDADLSEPTLIEGKSFREMTDAERDKISDKISVMGRSSPNDKLLLVQSLRRQGHVV AVTGDGTNDAPALHEADIGLAMGIAGTEVAKESSDIIILDDNFASVVKVVRWGRSVYANI QKFIQFQLTVNVAALVINVVAAISSGDVPLTAVQLLWVNLIMDTLGALALATEPPTDHLM GRPPVGRKEPLITNIMWRNLLIQAIYQVSVLLTLNFRGISILGLEHEVHEHATRVKNTII FNAFVLCQAFNEFNARKPDEKNIFKGVIKNRLFMGIIVITLVLQVIIVEFLGKFASTTKL NWKQWLICVGIGVISWPLALVGKFIPVPAAPISNKLKVLKFWGKKKNSSGEGSL ; 1314 . # loop_ _entity_src_gen.entity_id _entity_src_gen.gene_src_common_name 1017 . 1018 "Escherichia coli. (AL95 strain)" 1312 "Arabidopsis thaliana" 1314 "Arabidopsis thaliana" # loop_ _struct_ref.id _struct_ref.entity_id _struct_ref.db_name _struct_ref.db_code _struct_ref.details 1 1017 UniProt . http://www.uniprot.org/uniprot/Q9LF79 2 1018 UniProt . http://www.uniprot.org/uniprot/P59220