{ "code": "SASDUW7", "status": "Published", "type_of_curve": "SEC-SAS", "angular_unit": "1/A", "project": { "title": "The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures", "publication": { "title": "The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures", "author_list": "Bradshaw W, Harris G, Gileadi O, Katis V", "journal": "Structure", "doi": "10.1016/j.str.2024.09.024", "pmid": null, "published_date": "2024 Oct" }, "status": "released", "submitted_date": "2024-04-29", "released_date": "2024-10-07" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDUW7.dat", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDUW7.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUW7_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUW7_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDUW7_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUW7_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDUW7.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Eiger 4M", "resolution": 75.0 }, "name": "Diamond Light Source", "city": "Didcot", "country": "UK", "beamline_name": "B21", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Tyrosine-protein kinase SYK", "short_name": "SYK", "sequence": "SMADSANHLPFFFGNITREEAEDYLVQGGMSDGLYLLRQSRNYLGGFALSVAHGRKAHHYTIERELNGTYAIAGGRTHASPADLCHYHSQESDGLVCLLKKPFNRPQGVQPKTGPFEDLKENLIREYVKQTWNLQGQALEQAIISQKPQLEKLIATTAHEKMPWFHGKISREESEQIVLIGSKTNGKFLIRARDNNGSYALCLLHEGKVLHYRIDKDKTGKLSIPEGKKFDTLWQLVEHYSYKADGLLRVLTVPCQKIGTQGNVN", "organism": "Homo sapiens", "uniprot_code": "P43405", "uniprot_range_first": 6, "uniprot_range_last": 269, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MASSGMADSANHLPFFFGNITREEAEDYLVQGGMSDGLYLLRQSRNYLGGFALSVAHGRK\nAHHYTIERELNGTYAIAGGRTHASPADLCHYHSQESDGLVCLLKKPFNRPQGVQPKTGPF\nEDLKENLIREYVKQTWNLQGQALEQAIISQKPQLEKLIATTAHEKMPWFHGKISREESEQ\nIVLIGSKTNGKFLIRARDNNGSYALCLLHEGKVLHYRIDKDKTGKLSIPEGKKFDTLWQL\nVEHYSYKADGLLRVLTVPCQKIGTQGNVNFGGRPQLPGSHPATWSAGGIISRIKSYSFPK\nPGHRKSSPAQGNRQESTVSFNPYEPELAPWAADKGPQREALPMDTEVYESPYADPEEIRP\nKEVYLDRKLLTLEDKELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAE\nANVMQQLDNPYIVRMIGICEAESWMLVMEMAELGPLNKYLQQNRHVKDKNIIELVHQVSM\nGMKYLEESNFVHRDLAARNVLLVTQHYAKISDFGLSKALRADENYYKAQTHGKWPVKWYA\nPECINYYKFSSKSDVWSFGVLMWEAFSYGQKPYRGMKGSEVTAMLEKGERMGCPAGCPRE\nMYDLMNLCWTYDVENRPGFAAVELRLRNYYYDVVN", "mw": 29.862, "total_mw": 29.862, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" }, { "long_name": "TYRO protein tyrosine kinase-binding protein", "short_name": "TYROBP", "sequence": "ESPYQELQGQRSDVYSDLNT", "organism": "Homo sapiens", "uniprot_code": "O43914", "uniprot_range_first": 88, "uniprot_range_last": 107, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MGGLEPCSRLLLLPLLLAVSGLRPVQAQAQSDCSCSTVSPGVLAGIVMGDLVLTVLIALA\nVYFLGRLVPRGRGAAEAATRKQRITETESPYQELQGQRSDVYSDLNTQRPYYK", "mw": 2.489, "total_mw": 2.489, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" } ], "buffer": { "name": "10 mM HEPES, 150 mM NaCl, 1 mM TCEP", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "The tandem SH2 domains of Tyrosine-protein kinase SYK with a bound TYROBP diphospho-ITAM peptide", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Oxford, UK", "full_name": "University of Oxford", "webpage": "http://www.ox.ac.uk/" } ], "contributor_name": "William", "contributor_surname": "Bradshaw", "orcid": "https://orcid.org/0000-0002-7985-9752" } ], "concentration_method": null, "concentration_unit": null, "date": "2023-11-13", "storage_temperature": 15.0, "cell_temperature": 15.0, "exposure_time": 3.0, "number_of_frames": 600, "wavelength": 0.09408, "sample_detector_distance": 3.712, "concentration_min": null, "concentration_max": 10.0, "sample_volume": 45.0, "flow_rate": 0.08, "s_min": 0.117, "s_max": 2.32, "total_exposure_time": null, "seccolumn": 4 }, "fits": [], "estimated_volume_method": null, "pddf_software": "ScÅtter", "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of the tandem SH2 domains of Tyrosine-protein kinase SYK with a bound TYROBP diphospho-ITAM peptide in 10 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.09408 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample at 10 mg/ml was injected at a 0.08 ml/min flow rate onto a GE Superdex 75 Increase 5/150 column at 15°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.", "tags": [], "intensity_unit": "1/cm", "experimental_mw": 31.635, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 31.635, "porod_mw_error": null, "pddf_i0": 0.1440895, "pddf_i0_error": null, "guinier_i0": 0.0191493, "guinier_i0_error": null, "pddf_rg": 2.15, "pddf_rg_error": null, "guinier_rg": 2.143, "guinier_rg_error": 0.013, "pddf_dmax": 7.05, "pddf_dmax_error": null, "porod_volume": 52.51, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 362, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDUW7_datloglog_img.png", "symmetry": null, "last_modified": "2024-10-23T10:59:49.720798+02:00", "bragg_peak": [] }