data_SASDFX2_MAIN # _sas_result.id 1310 _sas_result.SASBDB_code SASDFX2 _sas_result.experimental_MW 29.920 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 24.6900000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 11.050 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 5 _sas_result.Guinier_point_max 15 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73A) polymer frame no. 305-309 ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 305-309)" _sas_scan.detc_id 15 _sas_scan.id 1310 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1271 _sas_scan.result_id 1310 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1271 _sas_sample.name "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 305-309)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1271 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 1.883960E+01 1.611328E+01 1310 2 5.968380E-03 1.736980E+01 8.414094E+00 1310 3 6.388220E-03 1.971480E+01 5.550196E+00 1310 4 6.808050E-03 2.355880E+01 4.392248E+00 1310 5 7.227880E-03 1.987340E+01 3.782118E+00 1310 6 7.647710E-03 2.098900E+01 2.965688E+00 1310 7 8.067540E-03 1.796790E+01 2.407498E+00 1310 8 8.487370E-03 1.896674E+01 2.531332E+00 1310 9 8.907200E-03 1.875342E+01 2.210124E+00 1310 10 9.327030E-03 1.618512E+01 1.912688E+00 1310 11 9.746860E-03 1.609162E+01 1.712736E+00 1310 12 1.016670E-02 1.576998E+01 1.725676E+00 1310 13 1.058650E-02 1.572234E+01 1.419832E+00 1310 14 1.100630E-02 1.574358E+01 1.423026E+00 1310 15 1.142620E-02 1.441526E+01 1.270924E+00 1310 16 1.184600E-02 1.311412E+01 1.243424E+00 1310 17 1.226580E-02 1.293542E+01 1.192210E+00 1310 18 1.268570E-02 1.254212E+01 1.128722E+00 1310 19 1.310550E-02 1.161654E+01 1.083512E+00 1310 20 1.352530E-02 1.159538E+01 9.393220E-01 1310 21 1.394520E-02 1.093026E+01 1.005061E+00 1310 22 1.436500E-02 1.026598E+01 9.412884E-01 1310 23 1.478480E-02 9.741240E+00 8.531308E-01 1310 24 1.520460E-02 8.904500E+00 8.772834E-01 1310 25 1.562450E-02 9.394480E+00 7.701360E-01 1310 26 1.604430E-02 8.470800E+00 7.624764E-01 1310 27 1.646410E-02 8.676160E+00 6.918312E-01 1310 28 1.688400E-02 8.762160E+00 6.604332E-01 1310 29 1.730380E-02 8.907180E+00 6.851466E-01 1310 30 1.772360E-02 7.376700E+00 6.015084E-01 1310 31 1.814350E-02 7.610280E+00 6.229094E-01 1310 32 1.856330E-02 7.311140E+00 5.764350E-01 1310 33 1.898310E-02 7.031140E+00 5.737352E-01 1310 34 1.940290E-02 6.533700E+00 5.400628E-01 1310 35 1.982280E-02 6.599060E+00 5.500992E-01 1310 36 2.024260E-02 6.178220E+00 5.220520E-01 1310 37 2.066240E-02 6.036200E+00 5.179544E-01 1310 38 2.108230E-02 5.609060E+00 5.014332E-01 1310 39 2.150210E-02 5.840500E+00 5.074900E-01 1310 40 2.192190E-02 5.628140E+00 4.663676E-01 1310 41 2.234180E-02 5.303020E+00 4.788120E-01 1310 42 2.276160E-02 5.140320E+00 4.634492E-01 1310 43 2.318140E-02 4.569920E+00 4.601248E-01 1310 44 2.360130E-02 4.681400E+00 4.310710E-01 1310 45 2.402110E-02 4.173980E+00 4.449254E-01 1310 46 2.444090E-02 4.260600E+00 4.281672E-01 1310 47 2.486070E-02 3.955460E+00 4.159672E-01 1310 48 2.528060E-02 4.146780E+00 4.152506E-01 1310 49 2.570040E-02 4.116400E+00 4.005828E-01 1310 50 2.612020E-02 3.759060E+00 4.032734E-01 1310 51 2.654010E-02 3.495200E+00 3.916508E-01 1310 52 2.695990E-02 3.545940E+00 3.909184E-01 1310 53 2.737970E-02 3.169680E+00 3.947660E-01 1310 54 2.779960E-02 3.478940E+00 3.904054E-01 1310 55 2.821940E-02 3.205560E+00 3.771518E-01 1310 56 2.863920E-02 2.992340E+00 3.793456E-01 1310 57 2.905900E-02 2.909560E+00 3.795776E-01 1310 58 2.947890E-02 2.937700E+00 3.842376E-01 1310 59 2.989870E-02 2.744040E+00 3.802516E-01 1310 60 3.031850E-02 2.872000E+00 3.685796E-01 1310 61 3.073840E-02 2.470580E+00 3.640538E-01 1310 62 3.115820E-02 2.601280E+00 3.688794E-01 1310 63 3.157800E-02 2.475500E+00 3.695216E-01 1310 64 3.199790E-02 2.545820E+00 3.585190E-01 1310 65 3.241770E-02 2.722320E+00 3.687568E-01 1310 66 3.283750E-02 2.366760E+00 3.592910E-01 1310 67 3.325730E-02 2.147260E+00 3.557598E-01 1310 68 3.367720E-02 2.319800E+00 3.554908E-01 1310 69 3.409700E-02 1.938340E+00 3.497836E-01 1310 70 3.451680E-02 1.856840E+00 3.582792E-01 1310 71 3.493670E-02 2.364020E+00 3.521188E-01 1310 72 3.535650E-02 2.229140E+00 3.455296E-01 1310 73 3.577630E-02 1.860700E+00 3.378798E-01 1310 74 3.619620E-02 2.025660E+00 3.447322E-01 1310 75 3.661600E-02 2.060420E+00 3.439048E-01 1310 76 3.703580E-02 1.782320E+00 3.346734E-01 1310 77 3.745560E-02 1.452720E+00 3.214522E-01 1310 78 3.787550E-02 1.982400E+00 3.291712E-01 1310 79 3.829530E-02 1.839640E+00 3.203872E-01 1310 80 3.871510E-02 1.762360E+00 3.148742E-01 1310 81 3.913500E-02 1.662320E+00 3.043940E-01 1310 82 3.955480E-02 1.662940E+00 3.104726E-01 1310 83 3.997460E-02 1.444440E+00 3.045170E-01 1310 84 4.039450E-02 1.423360E+00 3.058970E-01 1310 85 4.081430E-02 1.458980E+00 3.034472E-01 1310 86 4.123410E-02 1.341000E+00 2.896804E-01 1310 87 4.165390E-02 1.347620E+00 3.023390E-01 1310 88 4.207380E-02 1.311020E+00 2.891330E-01 1310 89 4.249360E-02 1.397720E+00 2.849180E-01 1310 90 4.291340E-02 1.437520E+00 2.865602E-01 1310 91 4.333330E-02 1.317100E+00 2.819296E-01 1310 92 4.375310E-02 1.369680E+00 2.888636E-01 1310 93 4.417290E-02 1.380540E+00 2.687732E-01 1310 94 4.459280E-02 1.073960E+00 2.590856E-01 1310 95 4.501260E-02 9.954000E-01 2.664128E-01 1310 96 4.543240E-02 1.124800E+00 2.730832E-01 1310 97 4.585220E-02 1.180660E+00 2.739226E-01 1310 98 4.627210E-02 1.185320E+00 2.626984E-01 1310 99 4.669190E-02 1.116080E+00 2.594434E-01 1310 100 4.711170E-02 1.315320E+00 2.616192E-01 1310 101 4.753160E-02 9.103196E-01 2.605360E-01 1310 102 4.795140E-02 1.063760E+00 2.574198E-01 1310 103 4.837120E-02 1.086700E+00 2.536490E-01 1310 104 4.879110E-02 1.130920E+00 2.550504E-01 1310 105 4.921090E-02 1.116300E+00 2.536744E-01 1310 106 4.963070E-02 8.340806E-01 2.516950E-01 1310 107 5.005050E-02 8.339800E-01 2.516966E-01 1310 108 5.047040E-02 9.999400E-01 2.491110E-01 1310 109 5.089020E-02 9.223598E-01 2.604858E-01 1310 110 5.131000E-02 9.511000E-01 2.474162E-01 1310 111 5.172990E-02 9.576600E-01 2.529750E-01 1310 112 5.214970E-02 8.241598E-01 2.459196E-01 1310 113 5.256950E-02 7.954004E-01 2.494590E-01 1310 114 5.298940E-02 6.689796E-01 2.418542E-01 1310 115 5.340920E-02 7.114398E-01 2.397324E-01 1310 116 5.382900E-02 7.873600E-01 2.437600E-01 1310 117 5.424890E-02 9.202798E-01 2.416810E-01 1310 118 5.466870E-02 6.850000E-01 2.443158E-01 1310 119 5.508850E-02 8.348000E-01 2.388798E-01 1310 120 5.550830E-02 8.452404E-01 2.404218E-01 1310 121 5.592820E-02 7.962598E-01 2.336132E-01 1310 122 5.634800E-02 7.773602E-01 2.334208E-01 1310 123 5.676780E-02 8.170800E-01 2.360090E-01 1310 124 5.718770E-02 6.510200E-01 2.259944E-01 1310 125 5.760750E-02 6.963602E-01 2.252030E-01 1310 126 5.802730E-02 6.537800E-01 2.276456E-01 1310 127 5.844720E-02 6.737202E-01 2.273126E-01 1310 128 5.886700E-02 6.593800E-01 2.261040E-01 1310 129 5.928680E-02 7.168798E-01 2.208550E-01 1310 130 5.970660E-02 6.667996E-01 2.272910E-01 1310 131 6.012650E-02 7.879200E-01 2.204622E-01 1310 132 6.054630E-02 6.769000E-01 2.198360E-01 1310 133 6.096610E-02 7.486400E-01 2.213674E-01 1310 134 6.138600E-02 3.900004E-01 2.143686E-01 1310 135 6.180580E-02 6.154804E-01 2.188648E-01 1310 136 6.222560E-02 5.641802E-01 2.190854E-01 1310 137 6.264550E-02 6.888000E-01 2.202430E-01 1310 138 6.306530E-02 5.796798E-01 2.184890E-01 1310 139 6.348510E-02 6.850400E-01 2.164350E-01 1310 140 6.390490E-02 5.689800E-01 2.231746E-01 1310 141 6.432480E-02 6.519200E-01 2.158556E-01 1310 142 6.474460E-02 5.155602E-01 2.209296E-01 1310 143 6.516440E-02 4.427400E-01 2.110486E-01 1310 144 6.558430E-02 4.449400E-01 2.141324E-01 1310 145 6.600410E-02 5.607800E-01 2.156554E-01 1310 146 6.642390E-02 5.480396E-01 2.097912E-01 1310 147 6.684380E-02 6.304800E-01 2.101856E-01 1310 148 6.726360E-02 5.727802E-01 2.097402E-01 1310 149 6.768340E-02 6.235800E-01 2.104892E-01 1310 150 6.810320E-02 5.206002E-01 2.104110E-01 1310 151 6.852310E-02 4.658600E-01 2.065690E-01 1310 152 6.894290E-02 4.255200E-01 2.060950E-01 1310 153 6.936270E-02 4.515405E-01 2.031620E-01 1310 154 6.978260E-02 4.891206E-01 2.036434E-01 1310 155 7.020240E-02 6.714600E-01 2.058662E-01 1310 156 7.062220E-02 3.351202E-01 2.036924E-01 1310 157 7.104210E-02 6.084200E-01 2.039826E-01 1310 158 7.146190E-02 2.545002E-01 1.978158E-01 1310 159 7.188170E-02 4.684796E-01 2.016720E-01 1310 160 7.230160E-02 6.012800E-01 1.995002E-01 1310 161 7.272140E-02 6.063794E-01 2.034222E-01 1310 162 7.314120E-02 4.920200E-01 1.995186E-01 1310 163 7.356100E-02 4.783006E-01 1.973288E-01 1310 164 7.398090E-02 5.275400E-01 2.005824E-01 1310 165 7.440070E-02 4.331402E-01 1.918814E-01 1310 166 7.482050E-02 4.242602E-01 1.975610E-01 1310 167 7.524040E-02 3.302204E-01 1.958466E-01 1310 168 7.566020E-02 4.373394E-01 1.912066E-01 1310 169 7.608000E-02 3.891800E-01 1.998176E-01 1310 170 7.649980E-02 3.251000E-01 1.935886E-01 1310 171 7.691970E-02 3.234601E-01 1.923288E-01 1310 172 7.733950E-02 4.484400E-01 1.917498E-01 1310 173 7.775930E-02 3.972604E-01 1.874918E-01 1310 174 7.817920E-02 5.163800E-01 1.965054E-01 1310 175 7.859900E-02 5.439402E-01 1.914760E-01 1310 176 7.901880E-02 3.837398E-01 1.944684E-01 1310 177 7.943870E-02 3.077401E-01 1.924326E-01 1310 178 7.985850E-02 5.094798E-01 1.927678E-01 1310 179 8.027830E-02 2.600202E-01 1.894646E-01 1310 180 8.069810E-02 5.243398E-01 1.896586E-01 1310 181 8.111800E-02 3.739004E-01 1.905988E-01 1310 182 8.153780E-02 4.560996E-01 1.903406E-01 1310 183 8.195760E-02 3.817200E-01 1.892462E-01 1310 184 8.237750E-02 2.782998E-01 1.890240E-01 1310 185 8.279730E-02 4.425600E-01 1.891902E-01 1310 186 8.321710E-02 3.519404E-01 1.932992E-01 1310 187 8.363700E-02 4.667795E-01 1.906732E-01 1310 188 8.405680E-02 4.422800E-01 1.967054E-01 1310 189 8.447660E-02 3.300397E-01 1.994538E-01 1310 190 8.489650E-02 5.290994E-01 2.033386E-01 1310 191 8.531630E-02 3.818200E-01 1.996234E-01 1310 192 8.573610E-02 3.570804E-01 2.041708E-01 1310 193 8.615590E-02 2.924800E-01 2.025026E-01 1310 194 8.657580E-02 3.141200E-01 2.000574E-01 1310 195 8.699560E-02 3.382408E-01 2.033776E-01 1310 196 8.741540E-02 3.785804E-01 2.053354E-01 1310 197 8.783530E-02 1.841001E-01 2.020226E-01 1310 198 8.825510E-02 3.363599E-01 2.029542E-01 1310 199 8.867490E-02 2.393206E-01 2.051376E-01 1310 200 8.909480E-02 2.463198E-01 2.036608E-01 1310 201 8.951460E-02 3.155798E-01 2.030966E-01 1310 202 8.993440E-02 8.363976E-02 2.004922E-01 1310 203 9.035420E-02 2.322605E-01 2.065428E-01 1310 204 9.077410E-02 1.869599E-01 2.006312E-01 1310 205 9.119390E-02 2.904999E-01 2.015348E-01 1310 206 9.161370E-02 2.392199E-01 2.050756E-01 1310 207 9.203360E-02 3.742400E-01 2.092710E-01 1310 208 9.245340E-02 2.036202E-01 2.035884E-01 1310 209 9.287320E-02 2.747401E-01 2.067186E-01 1310 210 9.329310E-02 2.717598E-01 2.108306E-01 1310 211 9.371290E-02 1.513595E-01 2.154394E-01 1310 212 9.413270E-02 2.022393E-01 2.079582E-01 1310 213 9.455250E-02 2.000797E-01 2.017058E-01 1310 214 9.497240E-02 2.976800E-01 2.036290E-01 1310 215 9.539220E-02 3.050800E-01 2.064616E-01 1310 216 9.581200E-02 2.427800E-01 2.014304E-01 1310 217 9.623190E-02 3.836200E-01 2.056884E-01 1310 218 9.665170E-02 3.191600E-01 2.018414E-01 1310 219 9.707150E-02 2.712594E-01 2.031424E-01 1310 220 9.749140E-02 8.957986E-02 1.978596E-01 1310 221 9.791120E-02 1.939202E-01 2.014942E-01 1310 222 9.833100E-02 1.661000E-01 2.036802E-01 1310 223 9.875080E-02 2.128602E-01 1.950608E-01 1310 224 9.917070E-02 1.255400E-01 1.956792E-01 1310 225 9.959050E-02 5.610004E-02 1.954764E-01 1310 226 1.000100E-01 1.474799E-01 1.938438E-01 1310 227 1.004300E-01 3.152603E-01 2.013646E-01 1310 228 1.008500E-01 3.987800E-01 1.939658E-01 1310 229 1.012700E-01 2.459402E-01 1.972002E-01 1310 230 1.016900E-01 2.308001E-01 1.946174E-01 1310 231 1.021090E-01 2.783202E-01 1.943806E-01 1310 232 1.025290E-01 2.707399E-01 1.960736E-01 1310 233 1.029490E-01 3.296596E-01 1.922446E-01 1310 234 1.033690E-01 2.825401E-01 1.949176E-01 1310 235 1.037890E-01 2.062194E-01 1.885580E-01 1310 236 1.042090E-01 3.582392E-01 1.944878E-01 1310 237 1.046280E-01 2.327804E-01 1.933588E-01 1310 238 1.050480E-01 5.242012E-02 1.899580E-01 1310 239 1.054680E-01 -5.220002E-03 1.899180E-01 1310 240 1.058880E-01 3.079602E-01 1.885432E-01 1310 241 1.063080E-01 3.072804E-01 1.918276E-01 1310 242 1.067280E-01 2.359802E-01 1.914532E-01 1310 243 1.071470E-01 2.139803E-01 1.868144E-01 1310 244 1.075670E-01 2.864806E-01 1.923600E-01 1310 245 1.079870E-01 3.074594E-01 1.887494E-01 1310 246 1.084070E-01 1.158803E-01 1.896690E-01 1310 247 1.088270E-01 1.378796E-01 1.868632E-01 1310 248 1.092470E-01 2.096601E-01 1.876686E-01 1310 249 1.096660E-01 1.054794E-01 1.852426E-01 1310 250 1.100860E-01 1.786798E-01 1.876866E-01 1310 251 1.105060E-01 3.098198E-01 1.907096E-01 1310 252 1.109260E-01 1.956399E-01 1.871978E-01 1310 253 1.113460E-01 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