{
  "code": "SASDWX2",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Structure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.",
    "publication": {
      "title": "Structure-guided disulfide engineering restricts antibody conformation to elicit TNFR agonism.",
      "author_list": "Elliott IG, Fisher H, Chan HTC, Inzhelevskaya T, Mockridge CI, Penfold CA, Duriez PJ, Orr CM, Herniman J, Müller KTJ, Essex JW, Cragg MS, Tews I",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-025-58773-8",
      "pmid": "40221417",
      "published_date": "2025 Apr 12"
    },
    "status": "released",
    "submitted_date": "2023-07-12",
    "released_date": "2025-02-25"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDWX2.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDWX2.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWX2_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWX2_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWX2_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWX2_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDWX2.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus3 2M",
        "resolution": 0.172
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "point",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Human immunoglobulin gamma 2 (IgG2) SAP1.3 - heavy chain C226S",
          "short_name": null,
          "sequence": "QVQLKQSGPGLVQPSQTLSITCTVSGFSLTSYGVHWVRQSPGKGLEWLGVIWSGGITDYNAAFISRLSISKDNSKSQVFFKVNSLQPNDTAIYYCARNGRIGSTMTLYYAMDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSNFGTQTYTCNVDHKPSNTKVDKTVERKSCVECPPCPAPPVAGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTFRVVSVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK",
          "organism": "Homo sapiens",
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "",
          "mw": 49.42,
          "total_mw": 98.84,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        },
        {
          "long_name": "Human immunoglobulin SAP1.3 - kappa chain C214S",
          "short_name": null,
          "sequence": "DIVMTQAAFSNPVTLGTSASISCRSSKSLLHSNGITYLYWYLQKPGQSPQLLIYQMSNLASGVPDRFSSSGSGTDFTLRISRVEAEDVGVYYCAQNLELPLTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGES",
          "organism": "Homo sapiens",
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "",
          "mw": 23.891,
          "total_mw": 47.782,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        }
      ],
      "buffer": {
        "name": "50 mM HEPES, 150 mM KCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Human immunoglobulin gamma 2 (IgG2) SAP1.3 - heavy chain C226S kappa chain C214S mutant",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Southampton, UK",
            "full_name": "University of Southampton",
            "webpage": "https://www.southampton.ac.uk/"
          }
        ],
        "contributor_name": "Isabel",
        "contributor_surname": "Elliott",
        "orcid": "https://orcid.org/0000-0003-4535-5932"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2024-09-28",
    "storage_temperature": 3.7,
    "cell_temperature": 20.0,
    "exposure_time": 2.0,
    "number_of_frames": 600,
    "wavelength": 0.099,
    "sample_detector_distance": 2.7,
    "concentration_min": null,
    "concentration_max": 9.3,
    "sample_volume": 45.0,
    "flow_rate": 0.25,
    "s_min": 0.06,
    "s_max": 4.959,
    "total_exposure_time": null,
    "seccolumn": 10
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "4.0.1-1",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 146.8,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 153.0,
  "porod_mw_error": null,
  "pddf_i0": 211.0,
  "pddf_i0_error": 0.2048,
  "guinier_i0": 211.116,
  "guinier_i0_error": null,
  "pddf_rg": 4.91,
  "pddf_rg_error": 0.006,
  "guinier_rg": 4.897,
  "guinier_rg_error": 0.092,
  "pddf_dmax": 15.8,
  "pddf_dmax_error": null,
  "porod_volume": 213.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 2,
  "guinier_point_last": 42,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDWX2_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-03-06T17:19:16.547548+01:00",
  "bragg_peak": []
}