{ "code": "SASDBY6", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "Structural and functional studies of the Leishmania braziliensis mitochondrial Hsp70: Similarities and dissimilarities to human orthologues.", "publication": { "title": "Structural and functional studies of the Leishmania braziliensis mitochondrial Hsp70: Similarities and dissimilarities to human orthologues.", "author_list": "Dores-Silva PR, Nishimura LS, Kiraly VT, Borges JC", "journal": "Arch Biochem Biophys", "doi": "10.1016/j.abb.2016.11.004", "pmid": "27840097", "published_date": "2017 Jan 1" }, "status": "released", "submitted_date": "2016-09-27", "released_date": "2016-11-17" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDBY6.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDBY6.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBY6_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBY6_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBY6_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBY6_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDBY6.sascif", "experiment": { "instrument": { "detector": { "type": "20Hz", "name": "Pilatus 300K", "resolution": null }, "name": "Brazilian Synchrotron Light Laboratory", "city": "Campinas", "country": "Brazil", "beamline_name": "SAXS1 Beamline", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Mitochondrial heat shock protein 70", "short_name": "LbmtHsp70", "sequence": "MGSSHHHHHHSSGLVPRGSHMESQKVTGDVIGVDLGTTYSCVATMDGDKARVLENSEGFRTTPSVVAFKGSEKLVGLAAKRQAITNPQSTFYAVKRLIGRRFEDEHIQRDIKNVPYKIVRAGNGDAWVQDGNGKQYSPSQVGAFVLEKMKETAQNFLGHTVSNAVVTCPAYFNDAQRQATKDAGTIAGLNVIRVVNEPTAAALAYGMDKTKDSLIAVYDLGGGTFDISVLEIAGGVFEVKATNGDTHLGGEDFDLALSDYILEEFRKTSGIDLSKERMALQRVREAAEKAKCELSSAMETEVNLPFITANADGAQHIQMHISRSKFEGITTRLIERSIAPCKQCIKDAGVELKEINDVVLVGGMTRMPKVVEEVKRFFQKEPFRGVNPDEAVALGAATLGGVLRGDVKGLVLLDVTPLSLGIETLGGVFTRMIPKNTTIPTKKSQTFSTAADNQTQVGIKVFQGEREMAADNQMMGQFDLVGIPPAPRGVPQVEVTFDIDANGICHVTAKDKATGKTQNITITAHGGLSKEQIEQMVRDSEQHAEADRVKRELVEARNNAETQLTTAERQLGEWKYVSDAEKENVKTHVAELRKAMENPNVAKDDLVAATDKLQKAVMECGRTEYQQAAAANSGSSSNSGEQQQQQQQSSEKN", "organism": "Leishmania braziliensis", "uniprot_code": null, "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": null, "mw": 70.902, "total_mw": 70.902, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "25 mM Tris-HCl, 50 mM NacL, 5 mM KCl, 5 mM sodium phosphate, 2 mM B-mercaptoethanol", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "Leishmania braziliensis Mitochondrial heat shock protein 70 (LbmtHSP70)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "São Carlos - SP - Brasil", "full_name": "Instituto de Química de São Carlos", "webpage": "" } ], "contributor_name": "Júlio", "contributor_surname": "Borges", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2012-06-22", "storage_temperature": 4.0, "cell_temperature": 25.0, "exposure_time": 60.0, "number_of_frames": 5, "wavelength": 0.1488, "sample_detector_distance": 1.0, "concentration_min": null, "concentration_max": 0.5, "sample_volume": null, "flow_rate": null, "s_min": 0.193, "s_max": 3.508, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDBY6_fit1_model1_img.png", "software": "DAMMIN", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDBY6_fit1_model1.pdb", "model_mw": 67.75, "bead_radius": 3.25, "log": "https://www.sasbdb.org/media/log_files/damsup.log", "symmetry": "", "comment": "", "user": 161 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBY6_fit1_fit_img.png", "software": null, "chi_square_value": 1.507, "p_value": 0.401, "fit_residual_plot": "SASDBY6_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDBY6_fit1.fir", "fit_log": null, "software_version": null, "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "GNOM - Version 4.5a", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of Leishmania braziliensis mitochondrial heat shock protein 70 (LbmtHSP70) in 25 mM Tris-HCl, 50 mM NaCL, 5 mM KCl, 5 mM sodium phosphate, 2 mM β-mercaptoethanol, pH 7.5, were collected on the SAXS1 Beamline at the Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a 20Hz Pilatus 300K detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.1488 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Scattering data from solute concentration of 0.5 mg/ml was measured at 25°C. Five successive 60 second data frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the radially-averaged scattering of the solvent-blank was subtracted.", "tags": [], "intensity_unit": null, "experimental_mw": 76.0, "experimental_mw_error": null, "guinier_i0_mw": 76.0, "guinier_i0_mw_error": 8.0, "porod_mw": 74.0, "porod_mw_error": null, "pddf_i0": 0.02592, "pddf_i0_error": 0.0001413, "guinier_i0": 0.025262101, "guinier_i0_error": 0.0025, "pddf_rg": 3.779, "pddf_rg_error": 0.032, "guinier_rg": 3.63, "guinier_rg_error": 0.2, "pddf_dmax": 14.0, "pddf_dmax_error": 1.0, "porod_volume": 118.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 14, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDBY6_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }