REMARK 265 REMARK 265 EXPERIMENT TYPE : X-RAY SOLUTION SCATTERING REMARK 265 DATA ACQUISITION REMARK 265 RADIATION SOURCE : BENDING MAGNET REMARK 265 SYNCHROTRON (Y/N) : Y REMARK 265 BEAMLINE : B21 DIAMOND LIGHT SOURCE REMARK 265 REMARK 265 DATA REDUCTION SOFTWARE : SCATTER (v3.0a) REMARK 265 SOFTWARE AUTHOR : RP RAMBO REMARK 265 DATA ANALYSIS SOFTWARE : SCATTER (v3.0a) REMARK 265 SOFTWARE AUTHOR : RP RAMBO REMARK 265 REMARK 265 EXPERIMENTAL INFO REMARK 265 NOTE : SEC-SAXS Signal Plot. 20.0 mg/ml of protein injected into a 4.6 ml Shodex KW403-4F size exclusion column at a flow rate of 0.16 mL min−1. REMARK 265 NOTE : Two-second frames in B21 using a Pilatus 2M pixel detector (DECTRIS) REMARK 265 REMARK 265 NOTE : REMARK 265 REMARK 265 EXPERIMENTAL DETAILS REMARK 265 BUFFER COMPOSITION REMARK 265 TEMPERATURE (KELVIN) : 298 REMARK 265 PH : 7.6 REMARK 265 SAMPLE BUFFER : 50 mM Tris-HCl REMARK 265 SAMPLE BUFFER :150 mM NaCl REMARK 265 SAMPLE BUFFER : 2 mM TCEP REMARK 265 REMARK 265 REMARK 265 COLUMNS : q, I(q), error REMARK 265 q : defined in inverse Angstroms 3.5565714E-03 7.30744E-02 1.47122E-02 3.7348571E-03 7.42307E-02 1.19398E-02 3.9131429E-03 5.82314E-02 9.35612E-03 4.0914286E-03 6.91828E-02 8.10361E-03 4.2697143E-03 7.81087E-02 6.58652E-03 4.448000E-03 6.52356E-02 5.44271E-03 4.6262857E-03 7.42166E-02 4.89950E-03 4.8045714E-03 7.09894E-02 4.13400E-03 4.9828571E-03 7.35909E-02 3.65293E-03 5.1611429E-03 7.50258E-02 3.23513E-03 5.3394286E-03 7.68631E-02 2.86488E-03 5.5177143E-03 7.95175E-02 2.64646E-03 5.696000E-03 7.58986E-02 2.39339E-03 5.8742857E-03 7.67397E-02 2.18164E-03 6.0525714E-03 7.88179E-02 2.05751E-03 6.2308571E-03 7.79820E-02 1.81901E-03 6.4091429E-03 7.77305E-02 1.67547E-03 6.5874286E-03 7.90058E-02 1.55603E-03 6.7657143E-03 7.67321E-02 1.47471E-03 6.944000E-03 7.42038E-02 1.35755E-03 7.1222857E-03 7.74214E-02 1.29335E-03 7.3005714E-03 7.73972E-02 1.19943E-03 7.4788571E-03 7.67984E-02 1.12027E-03 7.6571429E-03 7.69289E-02 1.05592E-03 7.8354286E-03 7.62083E-02 9.95798E-04 8.0137143E-03 7.69878E-02 9.51874E-04 8.192000E-03 7.62514E-02 9.09138E-04 8.3702857E-03 7.55164E-02 8.50149E-04 8.5485714E-03 7.63111E-02 8.13163E-04 8.7268571E-03 7.56243E-02 7.73054E-04 8.9051429E-03 7.47950E-02 7.41985E-04 9.0834286E-03 7.47088E-02 7.14372E-04 9.2617143E-03 7.42351E-02 6.83216E-04 9.440000E-03 7.45627E-02 6.50841E-04 9.6182857E-03 7.42890E-02 6.27652E-04 9.7965714E-03 7.36722E-02 6.01312E-04 9.9748571E-03 7.23639E-02 5.85433E-04 1.0153143E-02 7.35655E-02 5.71403E-04 1.0331429E-02 7.29080E-02 5.49011E-04 1.0509714E-02 7.39726E-02 5.47761E-04 1.068800E-02 7.40330E-02 5.28397E-04 1.0866286E-02 7.48632E-02 5.19107E-04 1.1044571E-02 7.45842E-02 5.11804E-04 1.1222857E-02 7.50882E-02 5.02924E-04 1.1401143E-02 7.49719E-02 4.91651E-04 1.1579429E-02 7.46324E-02 4.87519E-04 1.1757714E-02 7.49650E-02 4.64325E-04 1.193600E-02 7.57473E-02 4.59802E-04 1.2114286E-02 7.47089E-02 4.49715E-04 1.2292571E-02 7.38720E-02 4.44779E-04 1.2470857E-02 7.43619E-02 4.35525E-04 1.2649143E-02 7.48567E-02 4.33089E-04 1.2827429E-02 7.45837E-02 4.19209E-04 1.3005714E-02 7.47437E-02 4.19959E-04 1.318400E-02 7.48927E-02 4.08956E-04 1.3362286E-02 7.45657E-02 4.03542E-04 1.3540571E-02 7.47865E-02 4.04555E-04 1.3718857E-02 7.45084E-02 3.93694E-04 1.3897143E-02 7.39052E-02 3.86535E-04 1.4075429E-02 7.50674E-02 3.84270E-04 1.4253714E-02 7.41777E-02 3.75290E-04 1.443200E-02 7.39571E-02 3.75017E-04 1.4610286E-02 7.41163E-02 3.66601E-04 1.4788571E-02 7.42041E-02 3.60094E-04 1.4966857E-02 7.46298E-02 3.63731E-04 1.5145143E-02 7.34529E-02 3.51010E-04 1.5323429E-02 7.40355E-02 3.48950E-04 1.5501714E-02 7.42538E-02 3.47797E-04 1.568000E-02 7.39199E-02 3.40646E-04 1.5858286E-02 7.39073E-02 3.38859E-04 1.6036571E-02 7.35609E-02 3.36651E-04 1.6214857E-02 7.30966E-02 3.24368E-04 1.6393143E-02 7.36138E-02 3.27114E-04 1.6571429E-02 7.35700E-02 3.17439E-04 1.6749714E-02 7.32248E-02 3.14632E-04 1.692800E-02 7.32107E-02 3.14768E-04 1.7106286E-02 7.31792E-02 3.05281E-04 1.7284571E-02 7.28900E-02 2.96993E-04 1.7462857E-02 7.27616E-02 2.95378E-04 1.7641143E-02 7.34812E-02 2.85693E-04 1.7819429E-02 7.29565E-02 2.86959E-04 1.7997714E-02 7.30710E-02 2.82501E-04 1.817600E-02 7.30268E-02 2.77050E-04 1.8354286E-02 7.24203E-02 2.71761E-04 1.8532571E-02 7.21846E-02 2.68025E-04 1.8710857E-02 7.21926E-02 2.64691E-04 1.8889143E-02 7.24807E-02 2.65495E-04 1.9067429E-02 7.23258E-02 2.61185E-04 1.9245714E-02 7.27273E-02 2.60032E-04 1.942400E-02 7.15209E-02 2.58420E-04 1.9602286E-02 7.19515E-02 2.53749E-04 1.9780571E-02 7.24267E-02 2.53875E-04 1.9958857E-02 7.13165E-02 2.50537E-04 2.0137143E-02 7.18147E-02 2.49065E-04 2.0315429E-02 7.15632E-02 2.49435E-04 2.0493714E-02 7.09632E-02 2.45420E-04 2.067200E-02 7.11020E-02 2.41395E-04 2.0850286E-02 7.12124E-02 2.44417E-04 2.1028571E-02 7.14518E-02 2.37824E-04 2.1206857E-02 7.13512E-02 2.39460E-04 2.1385143E-02 7.14306E-02 2.38624E-04 2.1563429E-02 7.11589E-02 2.35582E-04 2.1741714E-02 7.10931E-02 2.34408E-04 2.192000E-02 7.05294E-02 2.32601E-04 2.2098286E-02 7.10744E-02 2.27892E-04 2.2276571E-02 7.03450E-02 2.31355E-04 2.2454857E-02 7.05411E-02 2.27271E-04 2.2633143E-02 7.02844E-02 2.26723E-04 2.2811429E-02 7.00244E-02 2.25118E-04 2.2989714E-02 7.03737E-02 2.22514E-04 2.316800E-02 7.03193E-02 2.21615E-04 2.3346286E-02 6.96052E-02 2.21542E-04 2.3524571E-02 7.02666E-02 2.19199E-04 2.3702857E-02 7.00272E-02 2.20221E-04 2.3881143E-02 6.98659E-02 2.17008E-04 2.4059429E-02 6.99344E-02 2.14392E-04 2.4237714E-02 6.98247E-02 2.15385E-04 2.441600E-02 6.92427E-02 2.13222E-04 2.4594286E-02 6.91756E-02 2.11529E-04 2.4772571E-02 6.92253E-02 2.12083E-04 2.4950857E-02 6.89923E-02 2.09266E-04 2.5129143E-02 6.93761E-02 2.08841E-04 2.5307429E-02 6.86768E-02 2.09277E-04 2.5485714E-02 6.90488E-02 2.03816E-04 2.566400E-02 6.83291E-02 2.06464E-04 2.5842286E-02 6.80778E-02 2.03084E-04 2.6020571E-02 6.83624E-02 2.03962E-04 2.6198857E-02 6.83732E-02 2.02846E-04 2.6377143E-02 6.84967E-02 2.00838E-04 2.6555429E-02 6.78679E-02 1.99713E-04 2.6733714E-02 6.79140E-02 2.00793E-04 2.691200E-02 6.76363E-02 1.97354E-04 2.7090286E-02 6.81333E-02 1.99557E-04 2.7268571E-02 6.76616E-02 1.97175E-04 2.7446857E-02 6.72249E-02 1.94964E-04 2.7625143E-02 6.73430E-02 1.94587E-04 2.7803429E-02 6.71074E-02 1.94765E-04 2.7981714E-02 6.70201E-02 1.91021E-04 2.816000E-02 6.70150E-02 1.92861E-04 2.8338286E-02 6.69371E-02 1.89774E-04 2.8516571E-02 6.67764E-02 1.90566E-04 2.8694857E-02 6.65822E-02 1.89411E-04 2.8873143E-02 6.68868E-02 1.88173E-04 2.9051429E-02 6.64735E-02 1.88091E-04 2.9229714E-02 6.60608E-02 1.86622E-04 2.940800E-02 6.62678E-02 1.85325E-04 2.9586286E-02 6.60152E-02 1.85418E-04 2.9764571E-02 6.58961E-02 1.85129E-04 2.9942857E-02 6.56003E-02 1.82699E-04 3.0121143E-02 6.54724E-02 1.83392E-04 3.0299429E-02 6.54747E-02 1.80864E-04 3.0477714E-02 6.54411E-02 1.81685E-04 3.065600E-02 6.52115E-02 1.80227E-04 3.0834286E-02 6.52069E-02 1.79444E-04 3.1012571E-02 6.50135E-02 1.78871E-04 3.1190857E-02 6.48312E-02 1.79240E-04 3.1369143E-02 6.48489E-02 1.76227E-04 3.1547429E-02 6.46610E-02 1.77366E-04 3.1725714E-02 6.45904E-02 1.75023E-04 3.190400E-02 6.41636E-02 1.75452E-04 3.2082286E-02 6.41262E-02 1.74555E-04 3.2260571E-02 6.41091E-02 1.74002E-04 3.2438857E-02 6.38249E-02 1.73521E-04 3.2617143E-02 6.39967E-02 1.73560E-04 3.2795429E-02 6.36267E-02 1.71119E-04 3.2973714E-02 6.36072E-02 1.73003E-04 3.315200E-02 6.35575E-02 1.71072E-04 3.3330286E-02 6.35096E-02 1.69034E-04 3.3508571E-02 6.33156E-02 1.69507E-04 3.3686857E-02 6.30323E-02 1.67703E-04 3.3865143E-02 6.32154E-02 1.68098E-04 3.4043429E-02 6.26086E-02 1.68250E-04 3.4221714E-02 6.23629E-02 1.66163E-04 3.440000E-02 6.24432E-02 1.65668E-04 3.4578286E-02 6.23857E-02 1.65229E-04 3.4756571E-02 6.20078E-02 1.63557E-04 3.4934857E-02 6.17423E-02 1.63871E-04 3.5113143E-02 6.18629E-02 1.62705E-04 3.5291429E-02 6.19965E-02 1.63729E-04 3.5469714E-02 6.20277E-02 1.62496E-04 3.564800E-02 6.15050E-02 1.61364E-04 3.5826286E-02 6.14277E-02 1.61142E-04 3.6004571E-02 6.11988E-02 1.61938E-04 3.6182857E-02 6.14128E-02 1.59377E-04 3.6361143E-02 6.08575E-02 1.60341E-04 3.6539429E-02 6.09017E-02 1.58923E-04 3.6717714E-02 6.07282E-02 1.57517E-04 3.689600E-02 6.06423E-02 1.57881E-04 3.7074286E-02 6.01492E-02 1.57032E-04 3.7252571E-02 6.04430E-02 1.56596E-04 3.7430857E-02 5.98814E-02 1.57129E-04 3.7609143E-02 6.04210E-02 1.55238E-04 3.7787429E-02 6.01740E-02 1.54908E-04 3.7965714E-02 5.96027E-02 1.54861E-04 3.814400E-02 5.95914E-02 1.53393E-04 3.8322286E-02 5.96724E-02 1.53266E-04 3.8500571E-02 5.93035E-02 1.53324E-04 3.8678857E-02 5.90554E-02 1.51686E-04 3.8857143E-02 5.90653E-02 1.51697E-04 3.9035429E-02 5.86953E-02 1.51050E-04 3.9213714E-02 5.86509E-02 1.51233E-04 3.939200E-02 5.85575E-02 1.50522E-04 3.9570286E-02 5.87206E-02 1.48562E-04 3.9748571E-02 5.84225E-02 1.49903E-04 3.9926857E-02 5.83827E-02 1.48942E-04 4.0105143E-02 5.77758E-02 1.48092E-04 4.0283429E-02 5.77768E-02 1.47581E-04 4.0461714E-02 5.75826E-02 1.47729E-04 4.064000E-02 5.75408E-02 1.46278E-04 4.0818286E-02 5.73551E-02 1.46842E-04 4.0996571E-02 5.69735E-02 1.45720E-04 4.1174857E-02 5.68844E-02 1.44592E-04 4.1353143E-02 5.70016E-02 1.45111E-04 4.1531429E-02 5.67468E-02 1.43877E-04 4.1709714E-02 5.67662E-02 1.43357E-04 4.188800E-02 5.66601E-02 1.44784E-04 4.2066286E-02 5.63814E-02 1.42231E-04 4.2244571E-02 5.62094E-02 1.42692E-04 4.2422857E-02 5.61946E-02 1.42143E-04 4.2601143E-02 5.59555E-02 1.42004E-04 4.2779429E-02 5.59166E-02 1.41861E-04 4.2957714E-02 5.55204E-02 1.40486E-04 4.313600E-02 5.54907E-02 1.40680E-04 4.3314286E-02 5.55354E-02 1.40721E-04 4.3492571E-02 5.54875E-02 1.39470E-04 4.3670857E-02 5.49321E-02 1.40594E-04 4.3849143E-02 5.50388E-02 1.41320E-04 4.4027429E-02 5.48949E-02 1.41119E-04 4.4205714E-02 5.44221E-02 1.41842E-04 4.438400E-02 5.43429E-02 1.41845E-04 4.4562286E-02 5.40447E-02 1.41949E-04 4.4740571E-02 5.40893E-02 1.41739E-04 4.4918857E-02 5.39574E-02 1.40722E-04 4.5097143E-02 5.36135E-02 1.40893E-04 4.5275429E-02 5.39186E-02 1.43031E-04 4.5453714E-02 5.34411E-02 1.40943E-04 4.563200E-02 5.33337E-02 1.41287E-04 4.5810286E-02 5.32188E-02 1.41757E-04 4.5988571E-02 5.29437E-02 1.40731E-04 4.6166857E-02 5.29423E-02 1.41272E-04 4.6345143E-02 5.25339E-02 1.40825E-04 4.6523429E-02 5.23429E-02 1.40479E-04 4.6701714E-02 5.22310E-02 1.40943E-04 4.688000E-02 5.21569E-02 1.39941E-04 4.7058286E-02 5.18399E-02 1.40112E-04 4.7236571E-02 5.20492E-02 1.40874E-04 4.7414857E-02 5.19575E-02 1.39436E-04 4.7593143E-02 5.16024E-02 1.40268E-04 4.7771429E-02 5.11399E-02 1.39306E-04 4.7949714E-02 5.11748E-02 1.39667E-04 4.812800E-02 5.10530E-02 1.39061E-04 4.8306286E-02 5.09328E-02 1.38466E-04 4.8484571E-02 5.04908E-02 1.38517E-04 4.8662857E-02 5.08791E-02 1.39818E-04 4.8841143E-02 5.03659E-02 1.37632E-04 4.9019429E-02 5.04623E-02 1.38042E-04 4.9197714E-02 5.00952E-02 1.37926E-04 4.937600E-02 4.99399E-02 1.37602E-04 4.9554286E-02 5.00027E-02 1.37926E-04 4.9732571E-02 4.96990E-02 1.36953E-04 4.9910857E-02 4.96434E-02 1.36938E-04 5.0089143E-02 4.93465E-02 1.36498E-04 5.0267429E-02 4.91263E-02 1.35117E-04 5.0445714E-02 4.89591E-02 1.35348E-04 5.062400E-02 4.88015E-02 1.33822E-04 5.0802286E-02 4.87748E-02 1.32167E-04 5.0980571E-02 4.84648E-02 1.31725E-04 5.1158857E-02 4.83753E-02 1.30222E-04 5.1337143E-02 4.80785E-02 1.30942E-04 5.1515429E-02 4.82616E-02 1.32648E-04 5.1693714E-02 4.79943E-02 1.31644E-04 5.187200E-02 4.79783E-02 1.32236E-04 5.2050286E-02 4.76687E-02 1.32223E-04 5.2228571E-02 4.75966E-02 1.29762E-04 5.2406857E-02 4.70799E-02 1.30279E-04 5.2585143E-02 4.71958E-02 1.31473E-04 5.2763429E-02 4.69924E-02 1.29029E-04 5.2941714E-02 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