{ "code": "SASDDZ2", "status": "Published", "type_of_curve": "SEC-SAS", "angular_unit": "1/nm", "project": { "title": "The structure of SALM5 suggests a dimeric assembly for the presynaptic RPTP ligand recognition.", "publication": { "title": "The structure of SALM5 suggests a dimeric assembly for the presynaptic RPTP ligand recognition.", "author_list": "Karki S, Paudel P, Sele C, Shkumatov AV, Kajander T", "journal": "Protein Eng Des Sel", "doi": "10.1093/protein/gzy012", "pmid": "29897575", "published_date": "2018 Jun 12" }, "status": "released", "submitted_date": "2018-01-11", "released_date": "2018-06-13" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDDZ2.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDDZ2.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDZ2_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDZ2_kratky_img.png", 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"MDKLCPLPCVCQNLSESLSTLCAHRGLLFVPPNVDRRTVELRLADNFIQALGPPDFRNMTGLVDLTLSRNAITRIGARSF\r\nGDLESLRSLHLDGNRLVELGSSSLRGPVNLQHLILSGNQLGRIAPGAFDDFLDSLEDLDVSYNNLRQVPWAGIGSMPALH\r\nTLNLDHNLIDALPPGVFAQLSQLSRLDLTSNRLATLAPDPLFSRGRDAEASPSPLVLSFSGNPLHCNCELLWLRRLARPD\r\nDLETCASPPTLAGRYFWAVPEGEFSCEPPLIARHTQRLWVLEGQRATLRCRALGDPVPTMHWVGPDDRLVGNSSRAWAFP\r\nNGTLEIGVTGAGDAGAYTCIATNPAGEATARVELRVLALPHGGNTSAEGGRPGPSDIAASARTAAEGEGTLESEPAVQVT\r\nEVTATSGLVSWGLGRPADPVWMFQIQYNSSEDETLIYRIVPASSHHFLLKHLVPGADYDLCLLALSPAAGPSDLTATRLL\r\nGCAHFSTLPATPLCHALQYPGILEVLFQ", "organism": "Mus musculus", "uniprot_code": "Q80XU8", "uniprot_range_first": 17, "uniprot_range_last": 510, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": 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7.5, "deuteration": null }, "purity_method": null, "name": "Leucine-rich repeat and fibronectin type-III domain-containing protein 4: Fragment SALM3 LRR-Ig-Fn", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "VUB", "address": "Pleinlaan 2 1050 Brussel ", "full_name": "Vrije Universiteit Brussel", "webpage": "http://www.vub.ac.be/" } ], "contributor_name": "Alexander", "contributor_surname": "Shkumatov", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2017-03-11", "storage_temperature": 4.7, "cell_temperature": 4.7, "exposure_time": 1.0, "number_of_frames": 1, "wavelength": 0.09919, "sample_detector_distance": 2.867, "concentration_min": null, "concentration_max": 22.3, "sample_volume": null, "flow_rate": null, "s_min": 0.093, "s_max": 3.504, "total_exposure_time": null, "seccolumn": null }, "fits": [ { 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"r9684", "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of Leucine-rich repeat and fibronectin type-III domain-containing protein 4 (fragment SALM3 LRR-Ig-Fn) in 20 mM Tris HCl, 100 mM NaCl, 0.02% NaN3, pH 7.5, were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). A solute concentration of 23.3 mg/ml was injected onto a size exclusion chromatography column and the eluted sample and buffer were measured at 4.7°C. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted to produce the SAXS data displayed in this entry.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 215.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 184.5, "porod_mw_error": null, "pddf_i0": 42.32, "pddf_i0_error": 0.05, "guinier_i0": 42.19, "guinier_i0_error": 0.076, "pddf_rg": 4.856, "pddf_rg_error": 0.01, "guinier_rg": 4.79, "guinier_rg_error": 0.01, "pddf_dmax": 17.09, "pddf_dmax_error": 1.5, "porod_volume": 313.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 17, "guinier_point_last": 39, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDDZ2_datloglog_img.png", "symmetry": null, "last_modified": "2020-08-28T15:27:45.357458+02:00", "bragg_peak": [] }