data_SASDFZ2_MAIN # _sas_result.id 1312 _sas_result.SASBDB_code SASDFZ2 _sas_result.experimental_MW 29.920 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 20.3300000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 12.130 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 2 _sas_result.Guinier_point_max 13 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2 ; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73A) polymer frame no. 315-319 ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 315-319)" _sas_scan.detc_id 15 _sas_scan.id 1312 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1273 _sas_scan.result_id 1312 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1273 _sas_sample.name "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 315-319)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1273 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 9.801208E+00 1.235371E+01 1312 2 5.968380E-03 1.589640E+01 7.228972E+00 1312 3 6.388220E-03 1.376940E+01 5.572112E+00 1312 4 6.808050E-03 1.489200E+01 4.206244E+00 1312 5 7.227880E-03 1.579380E+01 3.564906E+00 1312 6 7.647710E-03 1.716940E+01 3.038412E+00 1312 7 8.067540E-03 1.455750E+01 2.497498E+00 1312 8 8.487370E-03 1.477402E+01 2.337072E+00 1312 9 8.907200E-03 1.347832E+01 2.005530E+00 1312 10 9.327030E-03 1.268008E+01 1.916894E+00 1312 11 9.746860E-03 1.278962E+01 1.695566E+00 1312 12 1.016670E-02 1.261090E+01 1.472294E+00 1312 13 1.058650E-02 1.145612E+01 1.414080E+00 1312 14 1.100630E-02 1.155566E+01 1.409732E+00 1312 15 1.142620E-02 1.009506E+01 1.229828E+00 1312 16 1.184600E-02 9.108980E+00 1.104012E+00 1312 17 1.226580E-02 9.725440E+00 1.130578E+00 1312 18 1.268570E-02 1.018000E+01 1.042140E+00 1312 19 1.310550E-02 8.490680E+00 1.038966E+00 1312 20 1.352530E-02 8.069020E+00 9.459338E-01 1312 21 1.394520E-02 7.853060E+00 9.021746E-01 1312 22 1.436500E-02 8.188220E+00 8.907108E-01 1312 23 1.478480E-02 8.189380E+00 7.721528E-01 1312 24 1.520460E-02 7.324940E+00 8.175168E-01 1312 25 1.562450E-02 7.540140E+00 7.504150E-01 1312 26 1.604430E-02 7.562100E+00 7.249972E-01 1312 27 1.646410E-02 7.493980E+00 6.690764E-01 1312 28 1.688400E-02 6.677840E+00 6.504856E-01 1312 29 1.730380E-02 6.482040E+00 6.613288E-01 1312 30 1.772360E-02 5.993160E+00 5.936008E-01 1312 31 1.814350E-02 6.286920E+00 5.956520E-01 1312 32 1.856330E-02 5.555180E+00 5.343036E-01 1312 33 1.898310E-02 5.760240E+00 5.698000E-01 1312 34 1.940290E-02 6.209560E+00 5.505542E-01 1312 35 1.982280E-02 5.618920E+00 5.274190E-01 1312 36 2.024260E-02 5.148000E+00 5.375820E-01 1312 37 2.066240E-02 4.577440E+00 4.957372E-01 1312 38 2.108230E-02 4.473040E+00 4.892666E-01 1312 39 2.150210E-02 4.982300E+00 4.914052E-01 1312 40 2.192190E-02 4.494680E+00 4.685170E-01 1312 41 2.234180E-02 4.601580E+00 4.804036E-01 1312 42 2.276160E-02 4.162660E+00 4.404982E-01 1312 43 2.318140E-02 4.238160E+00 4.437724E-01 1312 44 2.360130E-02 4.312280E+00 4.422930E-01 1312 45 2.402110E-02 3.808160E+00 4.180476E-01 1312 46 2.444090E-02 3.632000E+00 4.322130E-01 1312 47 2.486070E-02 3.275760E+00 3.945864E-01 1312 48 2.528060E-02 3.456000E+00 4.084460E-01 1312 49 2.570040E-02 3.176400E+00 3.979238E-01 1312 50 2.612020E-02 3.157780E+00 3.951204E-01 1312 51 2.654010E-02 3.059060E+00 3.933264E-01 1312 52 2.695990E-02 3.051200E+00 3.795436E-01 1312 53 2.737970E-02 2.808880E+00 3.839976E-01 1312 54 2.779960E-02 2.582480E+00 3.727558E-01 1312 55 2.821940E-02 3.223180E+00 3.912626E-01 1312 56 2.863920E-02 2.690940E+00 3.812958E-01 1312 57 2.905900E-02 2.128400E+00 3.630316E-01 1312 58 2.947890E-02 2.834180E+00 3.873592E-01 1312 59 2.989870E-02 2.545680E+00 3.671658E-01 1312 60 3.031850E-02 2.527540E+00 3.687198E-01 1312 61 3.073840E-02 2.196580E+00 3.639742E-01 1312 62 3.115820E-02 2.309760E+00 3.627548E-01 1312 63 3.157800E-02 2.232460E+00 3.595858E-01 1312 64 3.199790E-02 2.420680E+00 3.605544E-01 1312 65 3.241770E-02 1.940860E+00 3.690394E-01 1312 66 3.283750E-02 2.034100E+00 3.603742E-01 1312 67 3.325730E-02 1.876540E+00 3.548288E-01 1312 68 3.367720E-02 1.960080E+00 3.508714E-01 1312 69 3.409700E-02 1.788080E+00 3.433078E-01 1312 70 3.451680E-02 2.012480E+00 3.590954E-01 1312 71 3.493670E-02 1.922300E+00 3.411932E-01 1312 72 3.535650E-02 1.940440E+00 3.434928E-01 1312 73 3.577630E-02 1.516420E+00 3.401674E-01 1312 74 3.619620E-02 1.788560E+00 3.391754E-01 1312 75 3.661600E-02 1.695840E+00 3.419856E-01 1312 76 3.703580E-02 1.610800E+00 3.267154E-01 1312 77 3.745560E-02 1.695280E+00 3.227662E-01 1312 78 3.787550E-02 1.640560E+00 3.199646E-01 1312 79 3.829530E-02 1.560140E+00 3.079186E-01 1312 80 3.871510E-02 1.717440E+00 3.180580E-01 1312 81 3.913500E-02 1.730380E+00 3.125774E-01 1312 82 3.955480E-02 1.562420E+00 3.094236E-01 1312 83 3.997460E-02 1.432440E+00 3.020986E-01 1312 84 4.039450E-02 1.188040E+00 3.015732E-01 1312 85 4.081430E-02 1.302820E+00 2.972598E-01 1312 86 4.123410E-02 1.335960E+00 2.995440E-01 1312 87 4.165390E-02 1.293260E+00 2.945536E-01 1312 88 4.207380E-02 1.358840E+00 2.857020E-01 1312 89 4.249360E-02 1.228660E+00 2.888768E-01 1312 90 4.291340E-02 1.168860E+00 2.926820E-01 1312 91 4.333330E-02 1.012600E+00 2.762476E-01 1312 92 4.375310E-02 1.220300E+00 2.790742E-01 1312 93 4.417290E-02 1.218600E+00 2.695534E-01 1312 94 4.459280E-02 1.083040E+00 2.740540E-01 1312 95 4.501260E-02 1.019320E+00 2.575652E-01 1312 96 4.543240E-02 9.989202E-01 2.716702E-01 1312 97 4.585220E-02 7.578398E-01 2.589586E-01 1312 98 4.627210E-02 1.105340E+00 2.582934E-01 1312 99 4.669190E-02 1.018600E+00 2.726636E-01 1312 100 4.711170E-02 1.003080E+00 2.649176E-01 1312 101 4.753160E-02 7.926000E-01 2.620082E-01 1312 102 4.795140E-02 9.218398E-01 2.587214E-01 1312 103 4.837120E-02 8.163798E-01 2.480006E-01 1312 104 4.879110E-02 1.021240E+00 2.565962E-01 1312 105 4.921090E-02 7.582800E-01 2.477678E-01 1312 106 4.963070E-02 8.289602E-01 2.522328E-01 1312 107 5.005050E-02 6.837000E-01 2.523112E-01 1312 108 5.047040E-02 7.881802E-01 2.543626E-01 1312 109 5.089020E-02 7.972198E-01 2.448372E-01 1312 110 5.131000E-02 8.354004E-01 2.486726E-01 1312 111 5.172990E-02 7.908200E-01 2.472458E-01 1312 112 5.214970E-02 1.021600E+00 2.439942E-01 1312 113 5.256950E-02 8.463204E-01 2.457472E-01 1312 114 5.298940E-02 5.739200E-01 2.398796E-01 1312 115 5.340920E-02 1.011900E+00 2.428752E-01 1312 116 5.382900E-02 8.320998E-01 2.371636E-01 1312 117 5.424890E-02 5.237198E-01 2.388384E-01 1312 118 5.466870E-02 7.084200E-01 2.413800E-01 1312 119 5.508850E-02 6.541000E-01 2.324290E-01 1312 120 5.550830E-02 6.760200E-01 2.351826E-01 1312 121 5.592820E-02 5.839996E-01 2.300404E-01 1312 122 5.634800E-02 8.730000E-01 2.387762E-01 1312 123 5.676780E-02 6.379400E-01 2.352578E-01 1312 124 5.718770E-02 5.965600E-01 2.241964E-01 1312 125 5.760750E-02 5.304404E-01 2.298224E-01 1312 126 5.802730E-02 5.020998E-01 2.258480E-01 1312 127 5.844720E-02 5.195602E-01 2.179966E-01 1312 128 5.886700E-02 5.647594E-01 2.220520E-01 1312 129 5.928680E-02 4.443200E-01 2.159212E-01 1312 130 5.970660E-02 4.819198E-01 2.258430E-01 1312 131 6.012650E-02 4.989600E-01 2.163714E-01 1312 132 6.054630E-02 4.621604E-01 2.172774E-01 1312 133 6.096610E-02 5.170600E-01 2.217628E-01 1312 134 6.138600E-02 5.443608E-01 2.203544E-01 1312 135 6.180580E-02 6.247400E-01 2.218208E-01 1312 136 6.222560E-02 4.695600E-01 2.155926E-01 1312 137 6.264550E-02 3.553196E-01 2.207218E-01 1312 138 6.306530E-02 6.889398E-01 2.151278E-01 1312 139 6.348510E-02 5.820398E-01 2.171528E-01 1312 140 6.390490E-02 6.146404E-01 2.186112E-01 1312 141 6.432480E-02 3.740400E-01 2.111378E-01 1312 142 6.474460E-02 2.976602E-01 2.075710E-01 1312 143 6.516440E-02 4.814200E-01 2.113348E-01 1312 144 6.558430E-02 5.344000E-01 2.101800E-01 1312 145 6.600410E-02 5.278997E-01 2.138350E-01 1312 146 6.642390E-02 5.341600E-01 2.093628E-01 1312 147 6.684380E-02 5.610400E-01 2.125770E-01 1312 148 6.726360E-02 3.240603E-01 2.068172E-01 1312 149 6.768340E-02 4.509200E-01 2.085638E-01 1312 150 6.810320E-02 4.141605E-01 2.045336E-01 1312 151 6.852310E-02 4.527600E-01 2.089960E-01 1312 152 6.894290E-02 4.541398E-01 2.058000E-01 1312 153 6.936270E-02 5.731208E-01 2.007800E-01 1312 154 6.978260E-02 4.408803E-01 2.060412E-01 1312 155 7.020240E-02 3.355200E-01 2.033712E-01 1312 156 7.062220E-02 4.828204E-01 2.000440E-01 1312 157 7.104210E-02 4.524800E-01 2.036614E-01 1312 158 7.146190E-02 2.683004E-01 1.945804E-01 1312 159 7.188170E-02 2.690194E-01 1.969084E-01 1312 160 7.230160E-02 3.932396E-01 1.979410E-01 1312 161 7.272140E-02 4.091395E-01 1.998778E-01 1312 162 7.314120E-02 4.791800E-01 2.005844E-01 1312 163 7.356100E-02 4.253006E-01 1.964886E-01 1312 164 7.398090E-02 3.799198E-01 2.004038E-01 1312 165 7.440070E-02 3.002807E-01 1.898524E-01 1312 166 7.482050E-02 5.496006E-01 1.980622E-01 1312 167 7.524040E-02 3.235802E-01 1.940256E-01 1312 168 7.566020E-02 2.162396E-01 1.885170E-01 1312 169 7.608000E-02 1.913199E-01 1.938124E-01 1312 170 7.649980E-02 3.734000E-01 1.915382E-01 1312 171 7.691970E-02 2.204799E-01 1.913126E-01 1312 172 7.733950E-02 2.899802E-01 1.924056E-01 1312 173 7.775930E-02 3.893600E-01 1.902484E-01 1312 174 7.817920E-02 3.634400E-01 1.947148E-01 1312 175 7.859900E-02 4.237402E-01 1.883862E-01 1312 176 7.901880E-02 2.810996E-01 1.942040E-01 1312 177 7.943870E-02 3.976398E-01 1.922562E-01 1312 178 7.985850E-02 4.195998E-01 1.906576E-01 1312 179 8.027830E-02 2.405001E-01 1.888844E-01 1312 180 8.069810E-02 3.188000E-01 1.878330E-01 1312 181 8.111800E-02 1.821601E-01 1.876844E-01 1312 182 8.153780E-02 1.638594E-01 1.884976E-01 1312 183 8.195760E-02 2.999599E-01 1.867478E-01 1312 184 8.237750E-02 3.181196E-01 1.900974E-01 1312 185 8.279730E-02 2.898598E-01 1.892916E-01 1312 186 8.321710E-02 3.765400E-01 1.915336E-01 1312 187 8.363700E-02 2.409798E-01 1.862382E-01 1312 188 8.405680E-02 3.393798E-01 1.959236E-01 1312 189 8.447660E-02 3.607800E-01 2.006508E-01 1312 190 8.489650E-02 1.284798E-01 1.963696E-01 1312 191 8.531630E-02 1.122000E-01 1.984998E-01 1312 192 8.573610E-02 2.467402E-01 2.016870E-01 1312 193 8.615590E-02 2.245199E-01 2.028940E-01 1312 194 8.657580E-02 3.839604E-01 2.032252E-01 1312 195 8.699560E-02 2.237204E-01 2.035294E-01 1312 196 8.741540E-02 3.562804E-01 2.027724E-01 1312 197 8.783530E-02 3.292407E-01 2.017422E-01 1312 198 8.825510E-02 2.121196E-01 2.047026E-01 1312 199 8.867490E-02 7.412035E-02 2.014682E-01 1312 200 8.909480E-02 1.898998E-01 2.032910E-01 1312 201 8.951460E-02 3.746000E-01 2.058620E-01 1312 202 8.993440E-02 2.738998E-01 1.971088E-01 1312 203 9.035420E-02 1.034205E-01 2.073584E-01 1312 204 9.077410E-02 1.158196E-01 2.030046E-01 1312 205 9.119390E-02 1.431002E-01 2.039438E-01 1312 206 9.161370E-02 1.719600E-01 2.059298E-01 1312 207 9.203360E-02 2.225801E-01 2.081156E-01 1312 208 9.245340E-02 9.944030E-02 2.059856E-01 1312 209 9.287320E-02 1.333405E-01 2.018086E-01 1312 210 9.329310E-02 2.712396E-01 2.095924E-01 1312 211 9.371290E-02 2.517196E-01 2.110464E-01 1312 212 9.413270E-02 1.510395E-01 2.069824E-01 1312 213 9.455250E-02 3.250198E-01 2.049956E-01 1312 214 9.497240E-02 1.740000E-01 2.043008E-01 1312 215 9.539220E-02 3.164400E-01 2.085920E-01 1312 216 9.581200E-02 2.524600E-01 2.014360E-01 1312 217 9.623190E-02 -3.725996E-02 2.045126E-01 1312 218 9.665170E-02 3.558033E-02 1.999158E-01 1312 219 9.707150E-02 1.711396E-01 2.008580E-01 1312 220 9.749140E-02 3.669978E-02 1.980242E-01 1312 221 9.791120E-02 1.961803E-01 1.934532E-01 1312 222 9.833100E-02 2.335401E-01 2.003186E-01 1312 223 9.875080E-02 1.771004E-01 1.959492E-01 1312 224 9.917070E-02 3.271402E-01 1.965186E-01 1312 225 9.959050E-02 1.577601E-01 1.964640E-01 1312 226 1.000100E-01 2.861799E-01 1.928162E-01 1312 227 1.004300E-01 6.906004E-02 1.983898E-01 1312 228 1.008500E-01 1.915799E-01 1.914338E-01 1312 229 1.012700E-01 1.883404E-01 1.977360E-01 1312 230 1.016900E-01 1.976800E-01 1.938628E-01 1312 231 1.021090E-01 3.423000E-01 1.929458E-01 1312 232 1.025290E-01 2.840395E-01 1.920822E-01 1312 233 1.029490E-01 1.440394E-01 1.854148E-01 1312 234 1.033690E-01 2.741201E-01 1.938012E-01 1312 235 1.037890E-01 1.676198E-01 1.921724E-01 1312 236 1.042090E-01 7.385974E-02 1.920478E-01 1312 237 1.046280E-01 1.397001E-01 1.902650E-01 1312 238 1.050480E-01 2.440000E-01 1.904032E-01 1312 239 1.054680E-01 1.480400E-01 1.942714E-01 1312 240 1.058880E-01 1.723001E-01 1.873460E-01 1312 241 1.063080E-01 1.423602E-01 1.915538E-01 1312 242 1.067280E-01 1.991804E-01 1.899810E-01 1312 243 1.071470E-01 8.360580E-03 1.909338E-01 1312 244 1.075670E-01 2.227405E-01 1.920920E-01 1312 245 1.079870E-01 1.168596E-01 1.848418E-01 1312 246 1.084070E-01 2.173002E-01 1.888106E-01 1312 247 1.088270E-01 1.117196E-01 1.857672E-01 1312 248 1.092470E-01 1.635199E-01 1.882604E-01 1312 249 1.096660E-01 1.694998E-01 1.869152E-01 1312 250 1.100860E-01 2.866594E-01 1.841028E-01 1312 251 1.105060E-01 1.942199E-01 1.911380E-01 1312 252 1.109260E-01 1.703399E-01 1.850088E-01 1312 253 1.113460E-01 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