{ "code": "SASDBZ4", "status": "Published", "type_of_curve": "Merged", "angular_unit": "1/nm", "project": { "title": "Stable preparations of tyrosine hydroxylase provide the solution structure of the full-length enzyme.", "publication": { "title": "Stable preparations of tyrosine hydroxylase provide the solution structure of the full-length enzyme.", "author_list": "Bezem MT, Baumann A, Skjærven L, Meyer R, Kursula P, Martinez A, Flydal MI", "journal": "Sci Rep", "doi": "10.1038/srep30390", "pmid": "27462005", "published_date": "2016 Jul 27" }, "status": "released", "submitted_date": "2016-06-25", "released_date": "2018-01-23" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDBZ4.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDBZ4.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBZ4_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBZ4_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBZ4_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBZ4_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDBZ4.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 2M", "resolution": null }, "name": "PETRA III", "city": "DESY; Hamburg", "country": "Germany", "beamline_name": "EMBL P12", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Tyrosine hydroxylase, isoform 1", "short_name": "TYH", "sequence": "MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIEDARKEREAAVAAAAAAV\r\nPSEPGDPLEAVAFEEKEGKAVLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQR\r\nPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLV\r\nTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLK\r\nGLYATHACGEHLEAFALLERFSGYREDNIPQLEDVSRFLKERTGFQLRPVAGLLSARDFL\r\nASLAFRVFQCTQYIRHASSPMHSPEPDCCHELLGHVPMLADRTFAQFSQDIGLASLGASD\r\nEEIEKLSTLYWFTVEFGLCKQNGEVKAYGAGLLSSYGELLHCLSEEPEIRAFDPEAAAVQ\r\nPYQDQTYQSVYFVSESFSDAKDKLRSYASRIQRPFSVKFDPYTLAIDVLDSPQAVRRSLE\r\nGVQDELDTLAHALSAIG", "organism": "Homo sapiens", "uniprot_code": "P07101", "uniprot_range_first": 1, "uniprot_range_last": 497, "oligomerization": "tetramer", "molecular_type": "protein", "uniprot_sequence": "MPTPDATTPQAKGFRRAVSELDAKQAEAIMVRGQGAPGPSLTGSPWPGTAAPAASYTPTP\nRSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAVLNLLFSPR\nATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVR\nQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEI\nAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLERFSGYREDNI\nPQLEDVSRFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCC\nHELLGHVPMLADRTFAQFSQDIGLASLGASDEEIEKLSTLYWFTVEFGLCKQNGEVKAYG\nAGLLSSYGELLHCLSEEPEIRAFDPEAAAVQPYQDQTYQSVYFVSESFSDAKDKLRSYAS\nRIQRPFSVKFDPYTLAIDVLDSPQAVRRSLEGVQDELDTLAHALSAIG", "mw": 55.611, "total_mw": 222.445, "number_molecules": 4, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM Na-HEPES 200 mM NaCl", "concentration_unit": "mM", "comment": null, "additive": "200 mM NaCl", "concentration": 20.0, "pka": null, "ph": 7.0, "deuteration": null }, "purity_method": null, "name": "Tyrosine hydroxylase (Isoform 1, Homo sapiens)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "UiB", "address": "Bergen, Norway", "full_name": "University of Bergen", "webpage": "http://www.uib.no" } ], "contributor_name": "Lars", "contributor_surname": "Skjærven", "orcid": null } ], "concentration_method": null, "concentration_unit": "mg/ml", "date": "2015-06-14", "storage_temperature": 20.0, "cell_temperature": 20.0, "exposure_time": 0.05, "number_of_frames": 20, "wavelength": 0.12, "sample_detector_distance": 3.1, "concentration_min": 1.0, "concentration_max": 3.0, "sample_volume": null, "flow_rate": null, "s_min": 0.027, "s_max": 4.801, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDBZ4_fit1_model1_img.png", "software": "BUNCH", "pdb_link": [ { "pdb_code": "1toh", "ext_code": "1TOH", "ext_type": "pdb", "difference_with_model": "derived" }, { "pdb_code": "2mda", "ext_code": "2MDA", "ext_type": "pdb", "difference_with_model": "derived" } ], "model_title": null, "type_of_model": "mix", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDBZ4_fit1_model1.pdb", "model_mw": 222.8, "bead_radius": 1.9, "log": null, "symmetry": "P222", "comment": null, "user": 134 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBZ4_fit1_fit_img.png", "software": "CRYSOL", "chi_square_value": 1.55, "p_value": 0.0, "fit_residual_plot": "SASDBZ4_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDBZ4_fit1.fit", "fit_log": null, "software_version": null, "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": "X-ray synchrotron radiation scattering data from solutions of tyrosine hydroxylase, isoform 1 in 20 mM Na-HEPES, 200 mM NaCl, pH 7.0 were collected on the P12 beam line of Petra-III (Hamburg, Germany) using a Pilatus 2M detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.124 nm). Different solute concentrations in the range 1.0-2.0 mg/ml were measured using an exposure time of 1 s (recorded as 20 x 0.050 s frames). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering from the matched solvent-blank was subtracted. The data presented here are merged SAXS data derived from the solute concentration series.", "tags": [], "intensity_unit": null, "experimental_mw": 283.8, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 63330.0, "pddf_i0_error": 219.0, "guinier_i0": 62494.4, "guinier_i0_error": 124.65, "pddf_rg": 4.93, "pddf_rg_error": 0.03, "guinier_rg": 4.74, "guinier_rg_error": 0.33, "pddf_dmax": 20.0, "pddf_dmax_error": null, "porod_volume": 519.8, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 40, "guinier_point_last": 95, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDBZ4_datloglog_img.png", "symmetry": null, "last_modified": "2023-06-21T07:56:39.492710+02:00", "bragg_peak": [] }