{
  "code": "SASDUZ5",
  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/nm",
  "project": {
    "title": "Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)",
    "publication": null,
    "status": "released",
    "submitted_date": "2024-03-27",
    "released_date": "2026-03-27"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDUZ5.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDUZ5.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUZ5_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUZ5_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDUZ5_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDUZ5_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDUZ5.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Glucokinase-1",
          "short_name": "GLK1",
          "sequence": "MSDPKLTKAVDSICDQFIVTKSKISQLTEYFIDCMEKGLEPCESDISQNKGLPMIPTFVTDKPSGQEHGVTMLAADLGGTNFRVCSVELLGNHEFKIEQEKSKIPTFFFQDDHHVTSKDLFQHMALITHQFLTKHHKDVIQDYKWKMGFTFSYPVDQTSLSSGKLIRWTKGFKIGDTVGQDVVQLFQQELNDIGLSNVHVVALTNDTTGTLLARCYASSDAARAINEPVIGCIFGTGTNGCYMEKLENIHKLDPASREELLSQGKTHMCINTEWGSFDNELNHLPTTSYDIKIDQQFSTNPGFHLFEKRVSGLYLGEILRNILLDLEKQELFDLKESVLKNNPFILTTETLSHIEIDTVENDLQDTRDALLKAADLETTFEERVLIQKLVRAISRRAAFLAAVPIAAILIKTNALNQSYHCQVEVGCDGSVVEHYPGFRSMMRHALALSPIGPEGERDVHLRISKDGSGVGAALCALHANY",
          "organism": "Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)",
          "uniprot_code": "Q6CUZ3",
          "uniprot_range_first": 1,
          "uniprot_range_last": 481,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSDPKLTKAVDSICDQFIVTKSKISQLTEYFIDCMEKGLEPCESDISQNKGLPMIPTFVT\nDKPSGQEHGVTMLAADLGGTNFRVCSVELLGNHEFKIEQEKSKIPTFFFQDDHHVTSKDL\nFQHMALITHQFLTKHHKDVIQDYKWKMGFTFSYPVDQTSLSSGKLIRWTKGFKIGDTVGQ\nDVVQLFQQELNDIGLSNVHVVALTNDTTGTLLARCYASSDAARAINEPVIGCIFGTGTNG\nCYMEKLENIHKLDPASREELLSQGKTHMCINTEWGSFDNELNHLPTTSYDIKIDQQFSTN\nPGFHLFEKRVSGLYLGEILRNILLDLEKQELFDLKESVLKNNPFILTTETLSHIEIDTVE\nNDLQDTRDALLKAADLETTFEERVLIQKLVRAISRRAAFLAAVPIAAILIKTNALNQSYH\nCQVEVGCDGSVVEHYPGFRSMMRHALALSPIGPEGERDVHLRISKDGSGVGAALCALHAN\nY",
          "mw": 53.757,
          "total_mw": 107.514,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        }
      ],
      "buffer": {
        "name": "10 mM Tris-HCl, 1 mM DTT",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Extrapolated scattering curve of dimeric Kluyveromyces lactis glucokinase 1 (KlGlk1)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "Leipzig University",
            "webpage": null
          }
        ],
        "contributor_name": "Renato H.",
        "contributor_surname": "Weiße",
        "orcid": "https://orcid.org/0000-0002-1736-8085"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2015-10-20",
    "storage_temperature": 10.0,
    "cell_temperature": 10.0,
    "exposure_time": 0.045,
    "number_of_frames": 30,
    "wavelength": 0.12399,
    "sample_detector_distance": 3.0,
    "concentration_min": 14.2,
    "concentration_max": 1.9,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.03,
    "s_max": 4.818,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDUZ5_fit1_model1_img.png",
          "software": "DAMMIN",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "dummy",
          "software_version": "ALPRAXIN 2.0 (r11734)",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDUZ5_fit1_model1.pdb",
          "model_mw": 86.955,
          "bead_radius": 3.25,
          "log": null,
          "symmetry": "P2",
          "comment": null,
          "user": 1050
        },
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDUZ5_fit1_model2_img.png",
          "software": "PyMOL",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": "2.5.5",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDUZ5_fit1_model2.pdb",
          "model_mw": 107.2,
          "bead_radius": null,
          "log": null,
          "symmetry": "P2",
          "comment": null,
          "user": 1050
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDUZ5_fit1_fixed_fit_img.png",
      "software": "DAMMIN",
      "chi_square_value": 1.571,
      "p_value": 0.0932,
      "fit_residual_plot": "SASDUZ5_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDUZ5_fit1.fir",
      "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/sym_p2_repr_prolate_1.9_across.log",
      "software_version": null,
      "description": ""
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": "Extropolation to infinite dilution using ALMERGE 1.1 (r11734) with scattering data of KlGlk1 with the following concentrations: 14.2 mg/mL, 7.3 mg/mL, 3.7 mg/mL, 1.9 mg/mL",
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "1/cm",
  "experimental_mw": 94.225,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 96.0,
  "porod_mw_error": null,
  "pddf_i0": 0.06502,
  "pddf_i0_error": null,
  "guinier_i0": 0.0660649,
  "guinier_i0_error": null,
  "pddf_rg": 3.289,
  "pddf_rg_error": null,
  "guinier_rg": 3.364,
  "guinier_rg_error": 0.003,
  "pddf_dmax": 9.94,
  "pddf_dmax_error": null,
  "porod_volume": 154.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 10,
  "guinier_point_last": 135,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDUZ5_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-03-27T11:00:12.571151+01:00",
  "bragg_peak": []
}