{ "code": "SASDCZ7", "status": "Published", "type_of_curve": "Merged", "angular_unit": "1/nm", "project": { "title": "Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo.", "publication": { "title": "Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo.", "author_list": "Ljubetič A, Lapenta F, Gradišar H, Drobnak I, Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R", "journal": "Nat Biotechnol", "doi": "10.1038/nbt.3994", "pmid": "29035374", "published_date": "2017 Nov" }, "status": "released", "submitted_date": "2017-09-28", "released_date": "2017-10-09" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDCZ7.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDCZ7.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCZ7_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCZ7_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCZ7_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCZ7_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDCZ7.sascif", "experiment": { "instrument": { "detector": { "type": "Custom-built", "name": "Pilatus 1M-W", "resolution": 0.172 }, "name": "PETRA III", "city": "DESY; Hamburg", "country": "Germany", "beamline_name": "EMBL P12", "beam_geometry": "Point", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "TET12(1.6)S-c6b", "short_name": null, "sequence": "MQLEDKVEELLSKNYHLENEVSRLKKLVGGDGKGSPEDEIQQLEEEISQLEQKNSQLKEKNQQLKYG GKGDGDIEQELERAKQSIRRLEQEVNQERSRMQYLQTLLSKGKGDGQLEDKVEELLSKNYHLENEVSRLKKLVGGKGDGLEEELKQLEEELQAIEEQLAQLQWKAQARKEKLAQLKEKLGKGDGSPEDENSQLEEKISQLKQKNSQLKEEIQQLEYGGKGDGDIEQELERAKQSIRRLEQEVNQERSRMQYLQTLLSKGKGDGSPEDEIQSLEEKNSQLKQEISQLEEKNQQLKYGGKGDGLEEELKQLEEELQAIEEQLAQLQWKAQARKE KLAQLKEKLGKGDGSPEDKISQLKQKIQQLKQENQQLEEENSQLEYGGDGKGSPEDKISQLKEENQQLEQKIQQLKEENSQLEYGGDGKGSPEDKNSQLKEEIQQLEEENQQLEEKISQLKYGLEHHHHHHHH", "organism": "synthetic construct", "uniprot_code": null, "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": null, "mw": 54.468, "total_mw": 54.468, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM Tris 150 mM NaCl 10% glycerol", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "TET12(1.6)S-c6b", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": null, "full_name": "National Research Council", "webpage": null } ], "contributor_name": "Jana", "contributor_surname": "Aupič", "orcid": "" } ], "concentration_method": null, "concentration_unit": null, "date": "2016-04-07", "storage_temperature": 20.0, "cell_temperature": 20.0, "exposure_time": 0.045, "number_of_frames": 20, "wavelength": 0.124, "sample_detector_distance": 2.0, "concentration_min": null, "concentration_max": 2.91, "sample_volume": null, "flow_rate": null, "s_min": 0.182, "s_max": 6.716, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDCZ7_fit1_model1_img.png", "software": "MODELLER", "pdb_link": [], "model_title": null, "type_of_model": "atomic", "software_version": "9.16", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDCZ7_fit1_model1.pdb", "model_mw": 54.43, "bead_radius": 1.9, "log": null, "symmetry": "", "comment": "", "user": 201 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCZ7_fit1_fit_img.png", "software": "Other", "chi_square_value": 1.01, "p_value": 0.0, "fit_residual_plot": "SASDCZ7_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDCZ7_fit1.fit", "fit_log": null, "software_version": "PepsiSAXS", "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of TET12(1.6)S-c6b in 20 mM Tris 150 mM NaCl 10% glycerol, pH 7.5 were collected on the P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 1M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5 mg/ml were measured at 20°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. Scattering curves were merged and analyzed using PRIMUS software.", "tags": [], "intensity_unit": null, "experimental_mw": 53.2, "experimental_mw_error": null, "guinier_i0_mw": 53.2, "guinier_i0_mw_error": 0.4, "porod_mw": 67.0, "porod_mw_error": null, "pddf_i0": 7157.0, "pddf_i0_error": 41.0, "guinier_i0": 7255.14, "guinier_i0_error": 29.0, "pddf_rg": 3.55, "pddf_rg_error": 0.02, "guinier_rg": 3.54, "guinier_rg_error": 0.05, "pddf_dmax": 11.5, "pddf_dmax_error": 1.0, "porod_volume": 114.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 33, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDCZ7_datloglog_img.png", "symmetry": null, "last_modified": "2023-06-21T07:56:18.120449+02:00", "bragg_peak": [] }