;Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN ;
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 500–946 | Not recorded | A1C4O (2R)-N-[(3R)-1,1-dioxo-1lambda~6~-thiolan-3-yl]-N-{[2-(2-hydroxypropan-2-yl)pyridin-4-yl]methyl}-2-methoxy-2-[(1M)-3,3',4'-trifluoro[1,1'-biphenyl]-4-yl]acetamide × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 1 EDO 1,2-ETHANEDIOL × 4 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION；277 K；co-crystallization additive 46 [Proplex-E2-B4+29% glycerol] | Resolution 2.58 Å R-free 0.286 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 10AP | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 10AJ Crystal Structure of Human WRN helicase with compound 1 Deposited 2026-01-08 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
518–946（429 aa）
|
Not recorded | ZN ZINC ION × 1 A1C4J 4-(4-methyl-4H-1,2,4-triazol-3-yl)-1-[4-(1H-pyrrol-1-yl)benzene-1-sulfonyl]piperidine × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；290 K；protocol was established using PROTEROS Standard Protocols
|
Resolution 2.42 Å R-free 0.254 |
| 10AK Crystal Structure of Human WRN helicase with compound 4 Deposited 2026-01-08 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
516–946（431 aa）
|
Not recorded | ZN ZINC ION × 1 A1C4L N-benzyl-N-[(3R)-1,1-dioxo-1lambda~6~-thiolan-3-yl]-2-[4-(1H-pyrrol-1-yl)phenyl]acetamide × 1 CL CHLORIDE ION × 2 EDO 1,2-ETHANEDIOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；290 K；protocol was established using PROTEROS Standard Protocols
|
Resolution 1.37 Å R-free 0.214 |
| 2AXL Solution structure of a multifunctional DNA- and protein-binding domain of human Werner syndrome protein Deposited 2005-09-05 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
949–1092（144 aa）
Fragment：The DPBD
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.4；295.2 K；Ionic strength (raw mmCIF value) 25 mM KPi + 25 mM NaCl；Pressure 1
NMR sample composition
0.35 mM in KPi buffer (pH7.4) 25 mM NaCl | 92.5% H2O/7.5% D2O
|
Resolution not provided |
| 2DGZ Solution structure of the Helicase and RNase D C-terminal domain in Werner syndrome ATP-dependent helicase Deposited 2006-03-16 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1140–1239（100 aa）
Fragment：Helicase and RNase D C-terminal domain
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 100mM；Pressure ambient
NMR sample composition
20mM d-Tris-HCl(pH7.0), 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2E1E Crystal structure of the HRDC Domain of Human Werner Syndrome Protein, WRN Deposited 2006-10-25 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1142–1242（101 aa）
Fragment：HRDC domain
|
Not recorded | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 9.5；293 K；pH 9.5, VAPOR DIFFUSION, temperature 293K
|
Resolution 2.30 Å R-free 0.279 |
| 2E1E Crystal structure of the HRDC Domain of Human Werner Syndrome Protein, WRN Deposited 2006-10-25 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1142–1242（101 aa）
Fragment：HRDC domain
|
Not recorded | CL CHLORIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 9.5；293 K；pH 9.5, VAPOR DIFFUSION, temperature 293K
|
Resolution 2.30 Å R-free 0.279 |
| 2E1F Crystal structure of the HRDC Domain of Human Werner Syndrome Protein, WRN Deposited 2006-10-25 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1142–1242（101 aa）
Fragment：HRDC domain
|
Not recorded | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.5；293 K；pH 5.5, VAPOR DIFFUSION, temperature 293K
|
Resolution 2.00 Å R-free 0.255 |
| 2E1F Crystal structure of the HRDC Domain of Human Werner Syndrome Protein, WRN Deposited 2006-10-25 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1142–1242（101 aa）
Fragment：HRDC domain
|
Not recorded | CL CHLORIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.5；293 K；pH 5.5, VAPOR DIFFUSION, temperature 293K
|
Resolution 2.00 Å R-free 0.255 |
| 2FBT WRN exonuclease Deposited 2005-12-10 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
38–236（199 aa）
Fragment：Exonculease domain
|
Not recorded | ACY ACETIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 4.5；277 K；5ul of 4.5mg/ml WRN exonuclease buffered in 25mM Tris HCl, 100mM NaCl, pH7.5, mixed with 5ul 1% MPEG 2K, 200mM Na Acetate, pH 4.5 from the reservoir solution and 1ul EDTA additive. , VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 4.50
|
Resolution 2.05 Å R-free 0.241 |
| 2FBV WRN exonuclease, Mn complex Deposited 2005-12-10 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
38–236（199 aa）
Fragment：Exonuclease domain
|
Not recorded | MN MANGANESE (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；277 K；5ul of 4.5mg/ml WRN exonuclease buffered in 25mM Tris HCL, 100mM NaCl, pH7.5, mixed with 5ul 1% MPEG 2K, 200mM Na Acetate, pH 4.5 from the reservior solution and 1ul EDTA additive, pH 4.50, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.40 Å R-free 0.277 |
| 2FBX WRN exonuclease, Mg complex Deposited 2005-12-10 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
38–236（199 aa）
Fragment：Exonuclease domain
|
Not recorded | MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；5ul of 4.5mg/ml WRN exonuclease buffered in 25mM Tris HCL, 100mM NaCl, pH 7.5, mixed with 5ul 1% MPEG 2K, 200mM Na Acetate, pH 4.5 from the reservior solution and 1ul EDTA additive, pH 4.50, VAPOR DIFFUSION, SITTING DROP
|
Resolution 2.20 Å R-free 0.262 |
| 2FBY WRN exonuclease, Eu complex Deposited 2005-12-10 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
38–236（199 aa）
Fragment：Exonuclease domain
|
Not recorded | EU3 EUROPIUM (III) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 4.5；5ul of 4.5mg/ml WRN exonuclease buffered in 25mM Tris HCL, 100mM NaCl, pH 7.5, mixed with 5ul 1% MPEG 2K, 200mM Na Acetate, pH 4.5 from the reservior solution and 1ul EDTA additive, VAPOR DIFFUSION, SITTING DROP, pH 4.50
|
Resolution 2.00 Å R-free 0.245 |
| 2FC0 WRN exonuclease, Mn dGMP complex Deposited 2005-12-10 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
38–236（199 aa）
Fragment：Exonuclease domain
|
Not recorded | MN MANGANESE (II) ION × 2 DGP 2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 4.5；277 K；5ul of 4.5mg/ml WRN exonuclease buffered in 25mM Tris HCl, 100mM NaCl, pH 7.5, mixed with 5ul 1% MPEG 2K, 200mM Na Acetate, pH 4.5 from the reservior solution and 1ul EDTA additive, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 4.50
|
Resolution 2.00 Å R-free 0.238 |
| 3AAF Structure of WRN RQC domain bound to double-stranded DNA Deposited 2009-11-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric |
Chain A
949–1079（131 aa）
Fragment：RecQ C-terminal (RQC) domain
Chain B
949–1079（131 aa）
Fragment：RecQ C-terminal (RQC) domain
|
Not recorded | ACT ACETATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 9；293 K；20% PEG 4000, 0.2M sodium acetate, 0.1M CAPS-NaOH, pH 9, VAPOR DIFFUSION, temperature 293K
|
Resolution 1.90 Å R-free 0.244 |
| 6YHR Crystal structure of Werner syndrome helicase Deposited 2020-03-30 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
517–1094（578 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；1M Na Acetate, 0.1 M Cacodylate pH 6.5
|
Resolution 2.20 Å R-free 0.236 |
| 7GQS Crystal Structure of Werner helicase fragment 517-945 in complex with ADP Deposited 2023-10-19 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
517–945（429 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 CD CADMIUM ION × 3 EDO 1,2-ETHANEDIOL × 2 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；277 K；5 mg/mL protein in 20mM TRIS/HCl pH7.5, 350mM NaCl, 0.25mM TCEP, 2.5% glycerol mixed 40nL with 180nL reservoir consisting of 9.091 %w/v Silver Bullets 42, 0.091 M MES monohydrate, 2.727 %v/v Jeffamine T-403, 13.6 %v/v pentaerythritol ethoxylate, 0.182M Potassium acetate pH6
|
Resolution 1.57 Å R-free 0.218 |
| 7GQT Crystal Structure of Werner helicase fragment 517-945 in complex with ATP Deposited 2023-10-19 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
517–945（429 aa）
|
Not recorded | ZN ZINC ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.2；277 K；5 mg/mL protein in 20mM TRIS/HCl pH7.5, 350mM NaCl, 0.25mM TCEP, 2.5% glycerol mixed 20nL with 20nL seed solution and 110nL reservoir consisting of 0.2M NaCl, 0.1M Na/K Phosphate, 10%w/v PEG8000
|
Resolution 2.21 Å R-free 0.263 |
| 7GQU Crystal Structure of Werner helicase fragment 517-945 in covalent complex with N-[(E,1S)-1-cyclopropyl-3-methylsulfonylprop-2-enyl]-2-(1,1-difluoroethyl)-4-phenoxypyrimidine-5-carboxamide Deposited 2023-10-19 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
517–945（429 aa）
|
Not recorded | ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 X1L N-[(1S)-1-cyclopropyl-3-(methanesulfonyl)propyl]-2-(1,1-difluoroethyl)-4-phenoxypyrimidine-5-carboxamide × 1 GOL GLYCEROL × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.2；293 K；5 mg/mL protein in 20mM TRIS/HCl pH7.5, 350mM NaCl, 0.25mM TCEP, 2.5% glycerol mixed 40nL with 180nL reservoir consisting of 20 %v/v Ethylene glycol, 11 %w/v PEG8000, 0.08M MES/NaOH pH6.2, 0.08M imidazole
|
Resolution 1.54 Å R-free 0.209 |
| 7XUT Crystal structure of RPA70N-WRN fusion Deposited 2022-05-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
435–451（17 aa）
Chain B
435–451（17 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；278 K；20% PEG3350, 200 mM ammonium sulfate
|
Resolution 1.60 Å R-free 0.214 |
| 8PFL Crystal structure of WRN helicase domain in complex with 3 Deposited 2023-06-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
517–945（429 aa）
|
Mutation：E625A, R564A, R785A, R803A, E886A, R942A | ZN ZINC ION × 1 YH8 2-[2-(3,6-dihydro-2~{H}-pyran-4-yl)-5-ethyl-7-oxidanylidene-6-[4-(3-oxidanylpyridin-2-yl)carbonylpiperazin-1-yl]-[1,2,4]triazolo[1,5-a]pyrimidin-4-yl]-~{N}-[2-methyl-4-(trifluoromethyl)phenyl]ethanamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30% Jeffamine ED-2001
0.1 M Hepes pH 7
|
Resolution 1.80 Å R-free 0.226 |
| 8PFO Crystal structure of WRN helicase domain in complex with HRO761 Deposited 2023-06-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
517–945（429 aa）
|
Mutation：E625A, R564A, R785A, R803A, E886A, R942A | ZN ZINC ION × 1 YHC ~{N}-[2-chloranyl-4-(trifluoromethyl)phenyl]-2-[2-(3,6-dihydro-2~{H}-pyran-4-yl)-5-ethyl-6-[4-(6-methyl-5-oxidanyl-pyrimidin-4-yl)carbonylpiperazin-1-yl]-7-oxidanylidene-[1,2,4]triazolo[1,5-a]pyrimidin-4-yl]ethanamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；25.5 % PEG 4000
0.17 M ammonium sulfate
15 % glycerol
|
Resolution 1.90 Å R-free 0.248 |
| 8PFP Crystal structure of WRN helicase domain in complex with ATPgammaS Deposited 2023-06-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
517–945（429 aa）
|
Mutation：E625A, R716A, K804A, E886A, R942A | ZN ZINC ION × 1 AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20 % PEG3350
0.2 M Sodium citrate tribasic dihydrate
|
Resolution 1.60 Å R-free 0.238 |
| 8YLE Crystal structure of Werner syndrome helicase complexed with AMP-PCP Deposited 2024-03-06 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
517–945（429 aa）
|
Not recorded | ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 EDO 1,2-ETHANEDIOL × 2 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.5；277 K；pH 6.5,0.1 M Amino acids,0.1 M Buffer System 1, 30.00% % v/v EDO_P8K
|
Resolution 1.86 Å R-free 0.214 |
| 9HZG Ku70/80 bound to WRN-exo Deposited 2025-01-13 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Heteromer；Protein × 3 PDB declaration: pentameric |
Chain E
1–1432（1432 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.33 Å |
| 9MJS Crystal structure of WRN helicase with bound AMPPNP Deposited 2024-12-16 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；277 K；30% Jeffamine ED 2003, 0.2 M NaCl, 0.2 M NDSB-201, 0.1 M MES
|
Resolution 1.84 Å R-free 0.225 |
| 9MJS Crystal structure of WRN helicase with bound AMPPNP Deposited 2024-12-16 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；277 K；30% Jeffamine ED 2003, 0.2 M NaCl, 0.2 M NDSB-201, 0.1 M MES
|
Resolution 1.84 Å R-free 0.225 |
| 9MJT Crystal structure of apo WRN helicase in Form D Deposited 2024-12-16 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 CL CHLORIDE ION × 1 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；21-27.5% w/v PEG1500, 0.1 M MMT, pH 5.75-6.25
|
Resolution 1.73 Å R-free 0.259 |
| 9MJU Crystal structure of WRN helicase with allosteric fragment 1 Deposited 2024-12-16 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 A1BL1 N-[4-(trifluoromethyl)phenyl]acetamide × 1 EDO 1,2-ETHANEDIOL × 4 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；20% v/v PEG6000, 0.1 M HEPES, pH 6.25-6.5
|
Resolution 1.58 Å R-free 0.243 |
| 9MJV Crystal structure of WRN helicase with bound allosteric fragment 2 Deposited 2024-12-16 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 A1BL2 2-[4-(trifluoromethoxy)phenyl]-2H-tetrazole-5-carboxylic acid × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；21-27.5% w/v PEG1500, 0.1 M MMT, pH 5.75-6.25
|
Resolution 2.58 Å R-free 0.288 |
| 9MJW Crystal structure of WRN helicase with bound allosteric fragment 3. Deposited 2024-12-16 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 A1BL3 6-chloro-7-methyl-1lambda~6~,2,4-benzothiadiazine-1,1,3(2H,4H)-trione × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；21%-27.5% (w/v) PEG 1500 and 0.1 M MMT pH 5.75-6.25
|
Resolution 2.00 Å R-free 0.263 |
| 9MJX Crystal structure of WRN helicase with bound allosteric fragment 4 Deposited 2024-12-16 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 A1BL4 (4R)-8-amino-2-cyclopropylimidazo[1,2-a]pyridine-6-carboxylic acid × 1 A1BL5 5,6-diaminopyridine-3-carboxylic acid × 1 SO4 SULFATE ION × 2 CL CHLORIDE ION × 3 EDO 1,2-ETHANEDIOL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；21%-27.5% (w/v) PEG 1500 and 0.1 M MMT pH 5.75-6.25
|
Resolution 1.73 Å R-free 0.229 |
| 9MJY Crystal structure of WRN helicase with bound allosteric compound 1 Deposited 2024-12-16 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 A1BL0 (3-{[4-(trifluoromethyl)phenyl]carbamoyl}furan-2-yl)acetic acid × 2 CL CHLORIDE ION × 5 EDO 1,2-ETHANEDIOL × 5 DMS DIMETHYL SULFOXIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；21%-27.5% (w/v) PEG 1500 and 0.1 M MMT pH 5.75-6.25
|
Resolution 1.63 Å R-free 0.222 |
| 9MJZ Crystal structure of WRN helicase with bound allosteric compound 3 Deposited 2024-12-16 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 A1BL6 (2-anilino-5-{[4-(trifluoromethyl)phenyl]carbamoyl}-1,3-thiazol-4-yl)acetic acid × 1 EDO 1,2-ETHANEDIOL × 8 GOL GLYCEROL × 1 DMS DIMETHYL SULFOXIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；21-27.5% w/v PEG1500, 0.1 M MMT, pH 5.75-6.25
|
Resolution 1.70 Å R-free 0.261 |
| 9MK0 Crystal structure of WRN helicase with bound allosteric compound 5 Deposited 2024-12-16 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
|
Not recorded | ZN ZINC ION × 1 A1BL7 (2-[4-(pyrimidine-4-carbonyl)piperazin-1-yl]-4-{[4-(trifluoromethyl)phenyl]carbamoyl}pyrimidin-5-yl)acetic acid × 1 EDO 1,2-ETHANEDIOL × 12 CL CHLORIDE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；20% w/v PEG6000, 0.2 M calcium chloride, 0.1 M MES, pH 6.0
|
Resolution 1.89 Å R-free 0.230 |
| 9MK1 Crystal structure of WRN helicase with bound compound 6 Deposited 2024-12-16 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 A1BL8 (2-[(2R)-2-(phenylcarbamoyl)-2,3-dihydro-1H-inden-4-yl]-5-{[4-(trifluoromethyl)phenyl]carbamoyl}-1,3-thiazol-4-yl)acetic acid × 1 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；24.5% w/v PEG1500, 0.1 M MMT, pH 6.0
|
Resolution 2.12 Å R-free 0.267 |
| 9OG3 Crystal structure of WRN in complex with compound 4 Deposited 2025-04-30 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
526–946（421 aa）
|
Not recorded | A1CA4 (3R,4S)-N-[(2R)-3-cyclohexyl-1-(dimethylamino)-1-oxopropan-2-yl]-1-[(2-fluoro-4-methylphenyl)acetyl]-4-[3-(2-oxo-2,3-dihydro-1H-1,3-benzimidazol-1-yl)propanamido]piperidine-3-carboxamide × 1 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；290.15 K；18% w/v PEG3350 and 0.18 M Tri-sodium citrate
|
Resolution 2.06 Å R-free 0.258 |
| 9OG8 Crystal structure of WRN in complex with compound 43 Deposited 2025-04-30 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
526–946（421 aa）
|
Not recorded | A1CA5 (3R,4S)-N-[(2R)-3-cyclohexyl-1-(methylamino)-1-oxopropan-2-yl]-4-{3-[6-(ethanesulfonamido)-2-oxo-2,3-dihydro-1H-1,3-benzimidazol-1-yl]propanamido}-1-[(2-fluoro-4-methylphenyl)acetyl]piperidine-3-carboxamide × 1 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；290.15 K；18.0% w/v PEG 3350, 0.18 M Tri-ammonium citrate
|
Resolution 1.66 Å R-free 0.239 |
| 9OW9 Human WRN helicase in complex with allosteric ligand Compound 1 Deposited 2025-06-02 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 A1CEV 2-[(9R)-1'-[(1,3-benzoxazol-2-yl)acetyl]-2-(morpholin-4-yl)-8-oxo-5,8-dihydro-4H-spiro[furo[3,4-d][1,2,4]triazolo[1,5-a]pyrimidine-7,4'-piperidin]-4-yl]-N-[2-chloro-4-(trifluoromethyl)phenyl]acetamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；23-24.5% w/v PEG1500, 100 mM MMT, pH 5.75-6.25
|
Resolution 2.34 Å R-free 0.301 |
| 9OWA Human WRN helicase in complex with allosteric ligand Compound 2 Deposited 2025-06-02 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 A1CEW N-[2-chloro-4-(trifluoromethyl)phenyl]-2-[(5'R,10'R)-2'-(3,6-dihydro-2H-pyran-4-yl)-1-(5-hydroxy-6-methylpyrimidine-4-carbonyl)-5'-methyl-9'-oxo-5',9'-dihydrospiro[piperidine-4,8'-pyrano[4,3-d][1,2,4]triazolo[1,5-a]pyrimidin]-4'(6'H)-yl]acetamide × 1 CL CHLORIDE ION × 1 EDO 1,2-ETHANEDIOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；23-24.5% w/v PEG1500, 100 mM MMT, pH 5.75-6.25
|
Resolution 1.98 Å R-free 0.306 |
| 9OWB Human WRN helicase in complex with allosteric ligand Compound 6 Deposited 2025-06-02 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 A1CEX N-[2-chloro-4-(trifluoromethyl)phenyl]-2-[(10'R)-1-(3-hydroxypyridine-2-carbonyl)-2'-(morpholin-4-yl)-9'-oxo-5',9'-dihydrospiro[piperidine-4,8'-pyrano[4,3-d][1,2,4]triazolo[1,5-a]pyrimidin]-4'(6'H)-yl]acetamide × 1 EDO 1,2-ETHANEDIOL × 7 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；20.0% w/v PEG3350, 200 mM ammonium tartrate
|
Resolution 1.56 Å R-free 0.192 |
| 9OWB Human WRN helicase in complex with allosteric ligand Compound 6 Deposited 2025-06-02 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 A1CEX N-[2-chloro-4-(trifluoromethyl)phenyl]-2-[(10'R)-1-(3-hydroxypyridine-2-carbonyl)-2'-(morpholin-4-yl)-9'-oxo-5',9'-dihydrospiro[piperidine-4,8'-pyrano[4,3-d][1,2,4]triazolo[1,5-a]pyrimidin]-4'(6'H)-yl]acetamide × 1 EDO 1,2-ETHANEDIOL × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；20.0% w/v PEG3350, 200 mM ammonium tartrate
|
Resolution 1.56 Å R-free 0.192 |
| 9OWB Human WRN helicase in complex with allosteric ligand Compound 6 Deposited 2025-06-02 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 A1CEX N-[2-chloro-4-(trifluoromethyl)phenyl]-2-[(10'R)-1-(3-hydroxypyridine-2-carbonyl)-2'-(morpholin-4-yl)-9'-oxo-5',9'-dihydrospiro[piperidine-4,8'-pyrano[4,3-d][1,2,4]triazolo[1,5-a]pyrimidin]-4'(6'H)-yl]acetamide × 1 EDO 1,2-ETHANEDIOL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；20.0% w/v PEG3350, 200 mM ammonium tartrate
|
Resolution 1.56 Å R-free 0.192 |
| 9OWB Human WRN helicase in complex with allosteric ligand Compound 6 Deposited 2025-06-02 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 A1CEX N-[2-chloro-4-(trifluoromethyl)phenyl]-2-[(10'R)-1-(3-hydroxypyridine-2-carbonyl)-2'-(morpholin-4-yl)-9'-oxo-5',9'-dihydrospiro[piperidine-4,8'-pyrano[4,3-d][1,2,4]triazolo[1,5-a]pyrimidin]-4'(6'H)-yl]acetamide × 1 EDO 1,2-ETHANEDIOL × 6 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；20.0% w/v PEG3350, 200 mM ammonium tartrate
|
Resolution 1.56 Å R-free 0.192 |
| 9OWC Human WRN helicase in complex with allosteric ligand Compound 7 Deposited 2025-06-02 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 A1CEY N-[2-chloro-4-(trifluoromethyl)phenyl]-2-[(5S,9R)-1'-(3-hydroxypyridine-2-carbonyl)-5-methyl-2-(morpholin-4-yl)-8-oxo-5,8-dihydrospiro[cyclopenta[d][1,2,4]triazolo[1,5-a]pyrimidine-7,4'-piperidin]-4(6H)-yl]acetamide × 1 EDO 1,2-ETHANEDIOL × 8 DMS DIMETHYL SULFOXIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；23-24.5% w/v PEG1500, 100 mM MMT, pH 5.75-6.25
|
Resolution 1.67 Å R-free 0.206 |
| 9OWD Human WRN helicase in complex with allosteric ligand Compound 9 Deposited 2025-06-02 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
Fragment：UNP residues 500-942
|
Not recorded | ZN ZINC ION × 1 A1CEZ N-[2-chloro-4-(trifluoromethyl)phenyl]-2-[(9R)-1'-(5-hydroxy-6-methylpyrimidine-4-carbonyl)-2-(morpholin-4-yl)-8-oxo-5,8-dihydrospiro[cyclopenta[d][1,2,4]triazolo[1,5-a]pyrimidine-7,4'-piperidin]-4(6H)-yl]acetamide × 1 EDO 1,2-ETHANEDIOL × 5 DMS DIMETHYL SULFOXIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；23-24.5% w/v PEG1500, 100 mM MMT, pH 5.75-6.25
|
Resolution 1.96 Å R-free 0.272 |
| 9QBU Crystal structure of WRN in complex with (S)-27 (GSK5819992) Deposited 2025-03-03 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
|
Not recorded | A1I5L 1-[(2~{S})-2-(2-azaspiro[3.3]heptan-2-ylcarbonyl)-4-[2-[4-(trifluoromethyl)phenyl]ethanoyl]piperazin-1-yl]propan-1-one × 1 EDO 1,2-ETHANEDIOL × 1 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277.15 K；7% PEG 20000, 0.1 M MES pH 5.5
|
Resolution 2.10 Å R-free 0.249 |
| 9QBU Crystal structure of WRN in complex with (S)-27 (GSK5819992) Deposited 2025-03-03 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
500–942（443 aa）
|
Not recorded | A1I5L 1-[(2~{S})-2-(2-azaspiro[3.3]heptan-2-ylcarbonyl)-4-[2-[4-(trifluoromethyl)phenyl]ethanoyl]piperazin-1-yl]propan-1-one × 1 EDO 1,2-ETHANEDIOL × 1 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277.15 K；7% PEG 20000, 0.1 M MES pH 5.5
|
Resolution 2.10 Å R-free 0.249 |
| 9QBV Crystal structure of WRN in complex with (R)-11 (GSK4766470) Deposited 2025-03-03 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
500–942（443 aa）
|
Not recorded | A1I5F 1-[(2~{R})-2-methyl-4-[2-[4-(trifluoromethyl)phenyl]ethanoyl]piperazin-1-yl]propan-1-one × 1 EDO 1,2-ETHANEDIOL × 6 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277.15 K；10% PEG 6000, 0.1 M HEPES pH 7.0
|
Resolution 2.15 Å R-free 0.258 |
| 9RTI Structure of WRN in complex with ATPgS and covalent ligand Compound 7 Deposited 2025-07-02 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
517–941（425 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 1 DMS DIMETHYL SULFOXIDE × 2 EDO 1,2-ETHANEDIOL × 15 A1JJ8 ~{N}-[1-[(4-fluorophenyl)methyl]-7-methyl-indazol-3-yl]propanamide × 1 A1JJQ ~{N}-[1-[(4-fluorophenyl)methyl]-7-methyl-indazol-3-yl]prop-2-enamide × 1 MLT D-MALATE × 1 PEG DI(HYDROXYETHYL)ETHER × 5 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；100 mM MMT pH 7.2, 25% w/v PEG 1500, 20% EG
|
Resolution 2.20 Å R-free 0.274 |
| 9RUR Structure of WRN in complex with ATPgS and covalent ligand Compound 4d Deposited 2025-07-04 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
517–941（425 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 1 DMS DIMETHYL SULFOXIDE × 3 EDO 1,2-ETHANEDIOL × 4 A1JJK 2-ethylsulfanyl-~{N}-[1-[(4-fluorophenyl)methyl]indazol-3-yl]ethanamide × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；0.1 mM MMT pH 7.2, 25 %w/v PEG 1500 and 20 %v/v EG
|
Resolution 2.30 Å R-free 0.281 |
| 9RUR Structure of WRN in complex with ATPgS and covalent ligand Compound 4d Deposited 2025-07-04 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
517–941（425 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 1 DMS DIMETHYL SULFOXIDE × 2 EDO 1,2-ETHANEDIOL × 4 ZN ZINC ION × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；0.1 mM MMT pH 7.2, 25 %w/v PEG 1500 and 20 %v/v EG
|
Resolution 2.30 Å R-free 0.281 |
| 9RUS Structure of WRN in complex with ATPgS and Compound 3 Deposited 2025-07-04 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
517–941（425 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 1 GQP 1-[(4-fluorophenyl)methyl]benzimidazole × 1 DMS DIMETHYL SULFOXIDE × 2 EDO 1,2-ETHANEDIOL × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；0.1 mM MMT pH 7.2, 25 %w/v PEG 1500
|
Resolution 2.19 Å R-free 0.298 |
| 9RUS Structure of WRN in complex with ATPgS and Compound 3 Deposited 2025-07-04 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
517–941（425 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 1 DMS DIMETHYL SULFOXIDE × 1 EDO 1,2-ETHANEDIOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；0.1 mM MMT pH 7.2, 25 %w/v PEG 1500
|
Resolution 2.19 Å R-free 0.298 |
| 9S17 WRN helicase in complex with molecule 81 Deposited 2025-07-18 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
517–941（425 aa）
|
Not recorded | ZN ZINC ION × 1 EDO 1,2-ETHANEDIOL × 5 A1JKV (2~{R},4~{R})-4-ethyl-~{N}-[(2~{S})-4-methylsulfonylbutan-2-yl]-2-phenyl-piperidine-1-carboxamide × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.8；293 K；100 mM BIS-TRIS 5.8 pH, 26 % w/v PEG 3350 and 275 mM LiSO4
|
Resolution 1.91 Å R-free 0.248 |
| 9S17 WRN helicase in complex with molecule 81 Deposited 2025-07-18 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
517–941（425 aa）
|
Not recorded | ZN ZINC ION × 1 EDO 1,2-ETHANEDIOL × 12 A1JKV (2~{R},4~{R})-4-ethyl-~{N}-[(2~{S})-4-methylsulfonylbutan-2-yl]-2-phenyl-piperidine-1-carboxamide × 1 SO4 SULFATE ION × 4 DMS DIMETHYL SULFOXIDE × 2 PEG DI(HYDROXYETHYL)ETHER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.8；293 K；100 mM BIS-TRIS 5.8 pH, 26 % w/v PEG 3350 and 275 mM LiSO4
|
Resolution 1.91 Å R-free 0.248 |
| 9S18 WRN helicase in complex with HRO761 Deposited 2025-07-18 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
517–943（427 aa）
|
Not recorded | FLC CITRATE ANION × 3 EDO 1,2-ETHANEDIOL × 4 YHC ~{N}-[2-chloranyl-4-(trifluoromethyl)phenyl]-2-[2-(3,6-dihydro-2~{H}-pyran-4-yl)-5-ethyl-6-[4-(6-methyl-5-oxidanyl-pyrimidin-4-yl)carbonylpiperazin-1-yl]-7-oxidanylidene-[1,2,4]triazolo[1,5-a]pyrimidin-4-yl]ethanamide × 1 DMS DIMETHYL SULFOXIDE × 1 PEG DI(HYDROXYETHYL)ETHER × 1 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；277 K；0.3M trisodium citrate tetrahydrate, 0.1M sodium citrate, pH 4.25 and 10% PEG 3350.
|
Resolution 2.00 Å R-free 0.251 |
| 9S19 WRN helicase in complex with ATPgS and ssDNA Deposited 2025-07-18 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric |
Chain A
517–941（425 aa）
|
Not recorded | ZN ZINC ION × 1 AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 1 EDO 1,2-ETHANEDIOL × 6 DMS DIMETHYL SULFOXIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；100mM HEPES pH 7.2 and 15% w/v PEG 8K
|
Resolution 2.30 Å R-free 0.257 |
| 9S19 WRN helicase in complex with ATPgS and ssDNA Deposited 2025-07-18 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric |
Chain B
517–941（425 aa）
|
Not recorded | ZN ZINC ION × 1 AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 1 EDO 1,2-ETHANEDIOL × 5 DMS DIMETHYL SULFOXIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；100mM HEPES pH 7.2 and 15% w/v PEG 8K
|
Resolution 2.30 Å R-free 0.257 |
| 9S1A WRN helicase in the absence of nucleotide or DNA Deposited 2025-07-18 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
517–941（425 aa）
|
Not recorded | ZN ZINC ION × 1 SO4 SULFATE ION × 2 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.8；293 K；100mM BIS-TRIS pH 5.8, 25% w/v PEG3350 and 200mM LiSO4.
|
Resolution 1.96 Å R-free 0.247 |
| 9S1A WRN helicase in the absence of nucleotide or DNA Deposited 2025-07-18 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
517–941（425 aa）
|
Not recorded | ZN ZINC ION × 1 SO4 SULFATE ION × 3 EDO 1,2-ETHANEDIOL × 6 DMS DIMETHYL SULFOXIDE × 2 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.8；293 K；100mM BIS-TRIS pH 5.8, 25% w/v PEG3350 and 200mM LiSO4.
|
Resolution 1.96 Å R-free 0.247 |
| 9S1B WRN helicase in complex with covalent inhibitor GSK_WRN3 Deposited 2025-07-18 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
517–943（427 aa）
|
Not recorded | ZN ZINC ION × 1 A1JKU ~{N}-[(3~{R})-1,1-bis(oxidanylidene)thiolan-3-yl]-5-oxidanyl-2-oxidanylidene-6-phenyl-1~{H}-pyridine-3-carboxamide × 1 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；277 K；0.3M trisodium citrate tetrahydrate, 0.1M sodium citrate, pH 4.25 and 10% PEG 3350
|
Resolution 2.22 Å R-free 0.294 |
49 other PDB entries and 61 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | WRN_HUMAN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–447； UniProt 500–946 |
