SAXSdb — 11hx

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1. Structure Basics

entry_id	11hx
deposition_date	2026-02-25
title	IscB-TID truncated and wRNA bound to Target ssRNA
keywords	IscB, Cas9, HNH, CRISPR-Cas, RNA BINDING PROTEIN, RNA BINDING PROTEIN-RNA complex; RNA BINDING PROTEIN/RNA
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	36.45 Å
rg_electron	35.77 Å
i0	399261000.00
molecular_weight	113230.0 kDa
excluded_volume	120930 Å³
envelope_volume	180770 Å³
shell_volume	43373 Å³
envelope_diameter	123.7 Å
shell_rg	40.67 Å
envelope_rg	35.66 Å
shape_rg	35.71 Å
total_rg	36.11 Å
total_atoms	7673
n_residues	629
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	116.1 Å
rg_real	36.47 Å
rg_real_error	0.85 Å
i0_real	3.9930e+08
i0_real_error	7.0900e+06
rg_reciprocal	36.46 Å
i0_reciprocal	399300000.0000
total_estimate	0.8951
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	37.8 Å
skewness	0.279
kurtosis	-0.558
angular_range	— – 0.2150 Å⁻¹
current_alpha	0.0000
highest_alpha	14840000.0000
n_real_points	44
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.955; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.974; Smooth: 0.795

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	IscB
formula_weight	50433.2 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	7673

MAVVYVISKSGKPLMPTTRCGHVRILLKEGKARVVERKPFTIQLTYESAEETQPLVLGIDPGRTNIGMSVVTESGESVFNAQIETRNKDVPKLMKDRKQYRMAHRRLKRRCKRRRRAKAAGTAFEEGEKQRLLPGCFKPITCKSIRNKEARFNNRKRPVGWLTPTANHLLVTHLNVVKKVQKILPVAKVVLELNRFSFMAMNNPKVQRWQYQRGPLYGKGSVEEAVSMQQDGHCLFCKHGIDEYHHVVPRRKNGSETLENRVGLCEEHNQLVHTDKEWEANLASKKSGMNKKYHALSVLNQIIPYLADQLADMFPGNFCVTSGQDTYLFREEHGIPKDHYLDAYCIACSALTDAKKVSSPKGRPYMVHQFRRHDRQACHKANLNRSYYMGGKLVATNRHKAMDQKTDSLEEYRAAHSAADVSKLTVKHPSAQYNGGLQIYV

Source

organism	synthetic construct
ncbi_taxonomy_id	32630
host_organism	;Escherichia coli 'BL21-Gold(DE3)pLysS AG' ;

Entity 2 polymer

description	wRNA (233-MER)
formula_weight	75414.8 kDa
src_method	man
entity_count	1

Polymer

type	polyribonucleotide
strand_id	B

AAAAGAGUGAACGAGAGGCUCUUUCAACUUGAAAAGGUUGAAAGAGCACAGGCUGAGACAUUCGUAAGGCCGAAAGGCCGGACGCACCCUGGGAUUUCCCCAGUCCCCGGAACUGCAUAGCGGAUGUCAGUUGAUCGGCCGAGUAAUUUACGUCGACGUUGACGUCGAUGGUUGCGGCCGAUCAGAUAAGCCAGGGGGAACAAUCACCUCUCUGGAAACAGAGAGAGUUUUAC

Source

organism	synthetic construct
ncbi_taxonomy_id	32630
host_organism	;Escherichia coli 'BL21-Gold(DE3)pLysS AG' ;

Entity 3 polymer

description	Target ssRNA
formula_weight	8643.0 kDa
src_method	syn
entity_count	1

Polymer

type	polyribonucleotide
strand_id	C

UCUUCUAGUCUCGUUCACUCUUUUCUCU

Entity 4 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	1

Entity 5 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.670 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.67 Å
resolution_method	—
num_particles	319480

fit	/app/data/saxs_computation/1h/11hx/11hx.fit
pddf	/app/data/pr_computation/1h/11hx/11hx.pr.out
profile	/app/data/saxs_computation/1h/11hx/11hx.dat

IscB-TID truncated and wRNA bound to Target ssRNA

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Entity 4 non-polymer

Entity 5 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)