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11ic

IscB-TID truncated and wRNA bound to Target ssRNA

Method: ELECTRON MICROSCOPY D_max: 117.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	11ic
deposition_date	2026-02-25
title	IscB-TID truncated and wRNA bound to Target ssRNA
keywords	IscB, Cas9, HNH, CRISPR-Cas, RNA BINDING PROTEIN, RNA BINDING PROTEIN-RNA complex; RNA BINDING PROTEIN/RNA
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	35.88 Å
rg_electron	35.46 Å
i0	357053000.00
molecular_weight	103740.0 kDa
excluded_volume	108820 Å³
envelope_volume	164180 Å³
shell_volume	40304 Å³
envelope_diameter	124.6 Å
shell_rg	39.75 Å
envelope_rg	35.36 Å
shape_rg	35.40 Å
total_rg	35.76 Å
total_atoms	11939
n_residues	537
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	117.5 Å
rg_real	35.97 Å
rg_real_error	0.85 Å
i0_real	3.5710e+08
i0_real_error	5.4370e+06
rg_reciprocal	35.92 Å
i0_reciprocal	357000000.0000
total_estimate	0.8880
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	37.4 Å
skewness	0.338
kurtosis	-0.527
angular_range	— – 0.2200 Å⁻¹
current_alpha	0.0000
highest_alpha	14060000.0000
n_real_points	45
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.915; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.947; Smooth: 0.848

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	IscB
formula_weight	50433.2 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	11939

MAVVYVISKSGKPLMPTTRCGHVRILLKEGKARVVERKPFTIQLTYESAEETQPLVLGIDPGRTNIGMSVVTESGESVFNAQIETRNKDVPKLMKDRKQYRMAHRRLKRRCKRRRRAKAAGTAFEEGEKQRLLPGCFKPITCKSIRNKEARFNNRKRPVGWLTPTANHLLVTHLNVVKKVQKILPVAKVVLELNRFSFMAMNNPKVQRWQYQRGPLYGKGSVEEAVSMQQDGHCLFCKHGIDEYHHVVPRRKNGSETLENRVGLCEEHNQLVHTDKEWEANLASKKSGMNKKYHALSVLNQIIPYLADQLADMFPGNFCVTSGQDTYLFREEHGIPKDHYLDAYCIACSALTDAKKVSSPKGRPYMVHQFRRHDRQACHKANLNRSYYMGGKLVATNRHKAMDQKTDSLEEYRAAHSAADVSKLTVKHPSAQYNGGLQIYV

Source

organism	synthetic construct
ncbi_taxonomy_id	32630
host_organism	;Escherichia coli 'BL21-Gold(DE3)pLysS AG' ;

Entity 2 polymer

description	wRNA (233-MER)
formula_weight	75414.8 kDa
src_method	man
entity_count	1

Polymer

type	polyribonucleotide
strand_id	B

AAAAGAGUGAACGAGAGGCUCUUUCAACUUGAAAAGGUUGAAAGAGCACAGGCUGAGACAUUCGUAAGGCCGAAAGGCCGGACGCACCCUGGGAUUUCCCCAGUCCCCGGAACUGCAUAGCGGAUGUCAGUUGAUCGGCCGAGUAAUUUACGUCGACGUUGACGUCGAUGGUUGCGGCCGAUCAGAUAAGCCAGGGGGAACAAUCACCUCUCUGGAAACAGAGAGAGUUUUAC

Source

organism	synthetic construct
ncbi_taxonomy_id	32630
host_organism	;Escherichia coli 'BL21-Gold(DE3)pLysS AG' ;

Entity 3 polymer

description	Target ssRNA
formula_weight	8643.0 kDa
src_method	syn
entity_count	1

Polymer

type	polyribonucleotide
strand_id	C

UCUUCUAGUCUCGUUCACUCUUUUCUCU

Entity 4 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	1

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.630 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.63 Å
resolution_method	—
num_particles	328824

fit	/app/data/saxs_computation/1i/11ic/11ic.fit
pddf	/app/data/pr_computation/1i/11ic/11ic.pr.out
profile	/app/data/saxs_computation/1i/11ic/11ic.dat

IscB-TID truncated and wRNA bound to Target ssRNA

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Entity 4 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)