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12e8

2E8 FAB FRAGMENT

Method: X-RAY DIFFRACTION D_max: 113.4 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	12e8
deposition_date	1998-03-14
title	2E8 FAB FRAGMENT
keywords	IMMUNOGLOBULIN; IMMUNOGLOBULIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	36.29 Å
rg_electron	35.90 Å
i0	145594000.00
molecular_weight	94748.0 kDa
excluded_volume	117450 Å³
envelope_volume	160090 Å³
shell_volume	38308 Å³
envelope_diameter	113.0 Å
shell_rg	41.14 Å
envelope_rg	35.04 Å
shape_rg	35.89 Å
total_rg	36.28 Å
total_atoms	6662
n_residues	856
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	113.4 Å
rg_real	36.31 Å
rg_real_error	0.86 Å
i0_real	1.4560e+08
i0_real_error	2.8100e+06
rg_reciprocal	36.30 Å
i0_reciprocal	145600000.0000
total_estimate	0.8773
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	46.4 Å
skewness	0.247
kurtosis	-0.632
angular_range	— – 0.2200 Å⁻¹
current_alpha	0.0000
highest_alpha	20550000.0000
n_real_points	45
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.966; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.964; Smooth: 0.539

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	IGG1-KAPPA 2E8 FAB (LIGHT CHAIN)
formula_weight	23541.0 kDa
src_method	nat
entity_count	2

Polymer

type	polypeptide(L)
strand_id	L,M
atom_count	7429

DIVMTQSQKFMSTSVGDRVSITCKASQNVGTAVAWYQQKPGQSPKLMIYSASNRYTGVPDRFTGSGSGTDFTLTISNMQSEDLADYFCQQYSSYPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSATDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC

Entity 2 polymer

description	IGG1-KAPPA 2E8 FAB (HEAVY CHAIN)
formula_weight	23878.6 kDa
src_method	nat
entity_count	2

Polymer

type	polypeptide(L)
strand_id	H,P

EVQLQQSGAEVVRSGASVKLSCTASGFNIKDYYIHWVKQRPEKGLEWIGWIDPEIGDTEYVPKFQGKATMTADTSSNTAYLQLSSLTSEDTAVYYCNAGHDYDRGRFPYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRD

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	767

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6. Citations (1)

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length_a	66.560 Å
length_b	65.570 Å
length_c	103.220 Å
angle_alpha	90.00 °
angle_beta	97.04 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	1.900 Å
resolution_low	20.000 Å
r_factor	0.2210
r_work	0.2210
r_free	0.2710

fit	/app/data/saxs_computation/2e/12e8/12e8.fit
pddf	/app/data/pr_computation/2e/12e8/12e8.pr.out
profile	/app/data/saxs_computation/2e/12e8/12e8.dat

2E8 FAB FRAGMENT

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)