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1a14

;COMPLEX BETWEEN NC10 ANTI-INFLUENZA VIRUS NEURAMINIDASE SINGLE CHAIN ANTIBODY WITH A 5 RESIDUE LINKER AND INFLUENZA VIRUS NEURAMINIDASE ;

Method: X-RAY DIFFRACTION D_max: 95.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1a14
deposition_date	1997-12-21
title	;COMPLEX BETWEEN NC10 ANTI-INFLUENZA VIRUS NEURAMINIDASE SINGLE CHAIN ANTIBODY WITH A 5 RESIDUE LINKER AND INFLUENZA VIRUS NEURAMINIDASE ;
keywords	COMPLEX (ANTIBODY-ANTIGEN), SINGLE-CHAIN ANTIBODY, GLYCOSYLATED PROTEIN, COMPLEX (ANTIBODY-ANTIGEN) complex; COMPLEX (ANTIBODY/ANTIGEN)
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	27.27 Å
rg_electron	26.69 Å
i0	85629900.00
molecular_weight	69936.0 kDa
excluded_volume	86115 Å³
envelope_volume	101590 Å³
shell_volume	31848 Å³
envelope_diameter	99.5 Å
shell_rg	33.84 Å
envelope_rg	27.08 Å
shape_rg	26.65 Å
total_rg	27.50 Å
total_atoms	5124
n_residues	612
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	95.8 Å
rg_real	27.31 Å
rg_real_error	0.79 Å
i0_real	8.5630e+07
i0_real_error	1.4400e+06
rg_reciprocal	27.30 Å
i0_reciprocal	85630000.0000
total_estimate	0.8627
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	29.8 Å
skewness	0.406
kurtosis	-0.289
angular_range	— – 0.2900 Å⁻¹
current_alpha	0.0000
highest_alpha	21230000.0000
n_real_points	59
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.752; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.962; Smooth: 0.993

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4. Crystallography & Experiment

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5. Entities & Polymer Info (7)

Entity 1 polymer

description	NEURAMINIDASE
formula_weight	43723.8 kDa
ec_number	3.2.1.18
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	N
atom_count	5124

RDFNNLTKGLCTINSWHIYGKDNAVRIGEDSDVLVTREPYVSCDPDECRFYALSQGTTIRGKHSNGTIHDRSQYRALISWPLSSPPTVYNSRVECIGWSSTSCHDGKTRMSICISGPNNNASAVIWYNRRPVTEINTWARNILRTQESECVCHNGVCPVVFTDGSATGPAETRIYYFKEGKILKWEPLAGTAKHIEECSCYGERAEITCTCRDNWQGSNRPVIRIDPVAMTHTSQYICSPVLTDNPRPNDPTVGKCNDPYPGNNNNGVKGFSYLDGVNTWLGRTISIASRSGYEMLKVPNALTDDKSKPTQGQTIVLNTDWSGYSGSFMDYWAEGECYRACFYVELIRGRPKEDKVWWTSNSIVSMCSSTEFLGQWDWPDGAKIEYFL

Entity 2 polymer

description	NC10 FV (HEAVY CHAIN)
formula_weight	13225.5 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	H

QVQLQQSGAELVKPGASVRMSCKASGYTFTNYNMYWVKQSPGQGLEWIGIFYPGNGDTSYNQKFKDKATLTADKSSNTAYMQLSSLTSEDSAVYYCARSGGSYRYDGGFDYWGQGTTVTV

Source

organism	Mus musculus
common_name	house mouse
ncbi_taxonomy_id	10090
host_organism	Escherichia coli

Entity 3 polymer

description	NC10 FV (LIGHT CHAIN)
formula_weight	11384.3 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	L

DIELTQTTSSLSASLGDRVTISCRASQDISNYLNWYQQNPDGTVKLLIYYTSNLHSEVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQDFTLPFTFGGGTAA

Source

organism	Mus musculus
common_name	house mouse
ncbi_taxonomy_id	10090
host_organism	Escherichia coli

Entity 4 branched

description	;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ;
formula_weight	1073.0 kDa
src_method	man
entity_count	1

Entity 5 non-polymer

description	alpha-D-mannopyranose
formula_weight	180.2 kDa
src_method	man
entity_count	1

Entity 6 non-polymer

description	2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	221.2 kDa
src_method	man
entity_count	2

Entity 7 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	1

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6. Citations (2)

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length_a	165.300 Å
length_b	165.300 Å
length_c	182.400 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	I 4 2 2
int_tables_number	97
crystal_system	—

resolution	2.500 Å
resolution_low	7.000 Å
r_factor	0.2000
r_work	0.2000
r_free	0.2700

fit	/app/data/saxs_computation/a1/1a14/1a14.fit
pddf	/app/data/pr_computation/a1/1a14/1a14.pr.out
profile	/app/data/saxs_computation/a1/1a14/1a14.dat

;COMPLEX BETWEEN NC10 ANTI-INFLUENZA VIRUS NEURAMINIDASE SINGLE CHAIN ANTIBODY WITH A 5 RESIDUE LINKER AND INFLUENZA VIRUS NEURAMINIDASE ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (7)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 branched

Entity 5 non-polymer

Entity 6 non-polymer

Entity 7 non-polymer

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)