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1auz

SOLUTION STRUCTURE OF SPOIIAA, A PHOSPHORYLATABLE COMPONENT OF THE SYSTEM THAT REGULATES TRANSCRIPTION FACTOR SIGMA-F OF BACILLUS SUBTILIS, NMR, 24 STRUCTURES

Method: SOLUTION NMR Dmax: 38.0 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

SPOIIAA

Bacillus subtilis

UniProt P10727

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 1–116 Not recorded No other associated polymer SOLUTION NMR NMR measurement conditions：pH 6.4；298 K；Pressure 1 NMR sample composition：1.0 mM SpoIIAA protein, 25 mM NaCl/25 mM K2HPO4/1 mM DTT, pH 7.0, 95% H2O/5% D2O | 95% H2O/5% D2O NMR sample composition：0.7 mM [U-15N] SpoIIAA protein, 25 mM NaCl/25 mM K2HPO4/1 mM DTT, pH 7.0, 95% H2O/5% D2O | 95% H2O/5% D2O NMR sample composition：0.7 mM [U-13C; U-15N] SpoIIAA protein, 25 mM NaCl/25 mM K2HPO4/1 mM DTT, pH 7.0, 95% H2O/5% D2O | 95% H2O/5% D2O Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1AUZ Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1BUZ SOLUTION STRUCTURE OF SPOIIAA, A PHOSPHORYLATABLE COMPONENT OF THE SYSTEM THAT REGULATES TRANSCRIPTION FACTOR SIGMA-F OF BACILLUS SUBTILIS NMR, MINIMIZED AVERAGE STRUCTURE Deposited 1997-09-08 Parsed fields agree Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–116（116 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.4；298 K；Pressure 1
NMR sample composition 1.0 mM SpoIIAA protein, 25 mM NaCl/25 mM K2HPO4/1 mM DTT, pH 7.0, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition 0.7 mM [U-15N] SpoIIAA protein, 25 mM NaCl/25 mM K2HPO4/1 mM DTT, pH 7.0, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition 0.7 mM [U-13C; U-15N] SpoIIAA protein, 25 mM NaCl/25 mM K2HPO4/1 mM DTT, pH 7.0, 95% H2O/5% D2O | 95% H2O/5% D2O
Resolution not provided

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name SP2AA_BACSU
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–116； UniProt 1–116

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1auz

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1auz
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2. Structure Basics 2. Structure Basics

Entry ID entry_id1auz
Deposition date deposition_date1997-09-08
Structure title titleSOLUTION STRUCTURE OF SPOIIAA, A PHOSPHORYLATABLE COMPONENT OF THE SYSTEM THAT REGULATES TRANSCRIPTION FACTOR SIGMA-F OF BACILLUS SUBTILIS, NMR, 24 STRUCTURES
Keywords keywordsTRANSCRIPTION REGULATOR, KINASE SUBSTRATE, ANTI-ANTI SIGMA FACTOR, NOVEL ALPHA/BETA FOLD; TRANSCRIPTION REGULATOR
Experimental Method methodSOLUTION NMR
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier13.56 Å
Radius of gyration Rg (electron density) rg_electron13.08 Å
Forward intensity I(0) i01256630000.00
Molecular weight molecular_weight308160.0 kDa
Excluded volume excluded_volume389330 ų
Envelope volume envelope_volume23379 ų
Hydration-shell volume shell_volume13713 ų
Envelope diameter envelope_diameter44.9 Å
Shell Rg shell_rg20.37 Å
Envelope Rg envelope_rg14.40 Å
Shape Rg shape_rg13.08 Å
Total Rg total_rg13.17 Å
Total atoms total_atoms40632
Residues n_residues2784
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax38.0 Å
Rg (real space) rg_real13.45 Å
Rg uncertainty (real space) rg_real_error0.17 Å
I(0) (real space) i0_real1.2570e+09
I(0) uncertainty (real space) i0_real_error1.2870e+07
Rg (reciprocal space) rg_reciprocal13.46 Å
I(0) (reciprocal space) i0_reciprocal1257000000.0000
Solution quality estimate total_estimate0.8691
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks0
Primary peak position r_peak_primary— Å
Skewness Skewness skewness-0.012
Kurtosis Kurtosis kurtosis-0.451
Angular range angular_range— – 0.5000 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha196200.0000
Real-space data points n_real_points80
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.981; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.975; Smooth: 0.378
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd1auza_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.13 — SpoIIaa-like
Superfamily Superfamily superfamilyc.13.2 — SpoIIaa-like
Family Family familyc.13.2.1 — Anti-sigma factor antagonist SpoIIaa

CATH v4.4 (1 domains)

Domain ID domain_id1auzA00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology750 — Transcription Regulator spoIIAA
Homologous superfamily homologous superfamily24 — STAS domain
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8. Citations (6)

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9. Files and Curves (10)