TEM-1 BETA LACTAMASE
Escherichia coli
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 24–286 | Mutation：VARIANT V84I, A184V | FOS [[N-(BENZYLOXYCARBONYL)AMINO]METHYL]PHOSPHATE × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.8；BUFFER NA/K-PHOSPHATE 20MM PH 7.8 AMMONIUM SULFATE 45% SATURATION (4 DEGREES) ACETONE 4% (V/V) PROTEIN 14.5 MG/ML | Resolution 2.00 Å R-free 0.206 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 1AXB | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1BT5 CRYSTAL STRUCTURE OF THE IMIPENEM INHIBITED TEM-1 BETA-LACTAMASE FROM ESCHERICHIA COLI Deposited 1998-09-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：V82I, A182V | SO4 SULFATE ION × 8 IM2 (5R)-5-[(1S,2R)-1-formyl-2-hydroxypropyl]-3-[(2-{[(E)-iminomethyl]amino}ethyl)sulfanyl]-4,5-dihydro-1H-pyrrole-2-carbox ylic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.8；pH 7.8
|
Resolution 1.80 Å R-free 0.238 |
| 1BTL CRYSTAL STRUCTURE OF ESCHERICHIA COLI TEM1 BETA-LACTAMASE AT 1.8 ANGSTROMS RESOLUTION Deposited 1993-11-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å |
| 1CK3 N276D MUTANT OF ESCHERICHIA COLI TEM-1 BETA-LACTAMASE Deposited 1999-04-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：V84I, A184V, N276D | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.8；pH 7.80
|
Resolution 2.28 Å R-free 0.235 |
| 1ERM X-RAY CRYSTAL STRUCTURE OF TEM-1 BETA LACTAMASE IN COMPLEX WITH A DESIGNED BORONIC ACID INHIBITOR (1R)-1-ACETAMIDO-2-(3-CARBOXYPHENYL)ETHANE BORONIC ACID Deposited 2000-04-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | BJI 1(R)-1-ACETAMIDO-2-(3-CARBOXYPHENYL)ETHYL BORONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.70 Å |
| 1ERO X-RAY CRYSTAL STRUCTURE OF TEM-1 BETA LACTAMASE IN COMPLEX WITH A DESIGNED BORONIC ACID INHIBITOR (1R)-2-PHENYLACETAMIDO-2-(3-CARBOXYPHENYL)ETHYL BORONIC ACID Deposited 2000-04-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Not recorded | BJP (1R)-2-PHENYLACETAMIDO-2-(3-CARBOXYPHENYL)ETHYL BORONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 2.10 Å |
| 1ERQ X-RAY CRYSTAL STRUCTURE OF TEM-1 BETA LACTAMASE IN COMPLEX WITH A DESIGNED BORONIC ACID INHIBITOR (1R)-1-ACETAMIDO-2-(3-CARBOXY-2-HYDROXYPHENYL)ETHYL BORONIC ACID Deposited 2000-04-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Not recorded | BJH 1(R)-1-ACETAMIDO-2-(3-CARBOXY-2-HYDROXYPHENYL)ETHYL BORONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.90 Å |
| 1ESU S235A MUTANT OF TEM1 BETA-LACTAMASE Deposited 2000-04-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：S235A | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；292 K；Imidazole 0.1M pH7.0, ammonium sulfate 43 to 48%, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.00 Å |
| 1FQG MOLECULAR STRUCTURE OF THE ACYL-ENZYME INTERMEDIATE IN TEM-1 BETA-LACTAMASE Deposited 2000-09-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：GLU166ASN MUTATION | PNM OPEN FORM - PENICILLIN G × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；1.4M sodium potassium phosphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.70 Å |
| 1JTD Crystal structure of beta-lactamase inhibitor protein-II in complex with TEM-1 beta-lactamase Deposited 2001-08-20 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
24–286（263 aa）
|
Not recorded | CA CALCIUM ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；PEG8000, calcium acetate, sodium cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.230 |
| 1JTG CRYSTAL STRUCTURE OF TEM-1 BETA-LACTAMASE / BETA-LACTAMASE INHIBITOR PROTEIN COMPLEX Deposited 2001-08-20 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
24–286（263 aa）
|
Mutation：I84V,V184A | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.8；pH 8.8
|
Resolution 1.73 Å R-free 0.205 |
| 1JTG CRYSTAL STRUCTURE OF TEM-1 BETA-LACTAMASE / BETA-LACTAMASE INHIBITOR PROTEIN COMPLEX Deposited 2001-08-20 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
24–286（263 aa）
|
Mutation：I84V,V184A | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.8；pH 8.8
|
Resolution 1.73 Å R-free 0.205 |
| 1JVJ CRYSTAL STRUCTURE OF N132A MUTANT OF TEM-1 BETA-LACTAMASE IN COMPLEX WITH A N-FORMIMIDOYL-THIENAMYCINE Deposited 2001-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM-1
|
Mutation：N132A | K POTASSIUM ION × 5 IM2 (5R)-5-[(1S,2R)-1-formyl-2-hydroxypropyl]-3-[(2-{[(E)-iminomethyl]amino}ethyl)sulfanyl]-4,5-dihydro-1H-pyrrole-2-carbox ylic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium Phospate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.73 Å R-free 0.193 |
| 1JWP Structure of M182T mutant of TEM-1 beta-lactamase Deposited 2001-09-04 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM-1
|
Mutation：M182T | PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.75 Å R-free 0.220 |
| 1JWV Crystal structure of G238A mutant of TEM-1 beta-lactamase in complex with a boronic acid inhibitor (sefb4) Deposited 2001-09-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM-1
|
Mutation：G238A | K POTASSIUM ION × 5 CB4 PINACOL[[2-AMINO-ALPHA-(1-CARBOXY-1-METHYLETHOXYIMINO)-4-THIAZOLEACETYL]AMINO]METHANEBORONATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.85 Å R-free 0.203 |
| 1JWZ Crystal structure of TEM-64 beta-lactamase in complex with a boronic acid inhibitor (105) Deposited 2001-09-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM-64
|
Mutation：E104K/M182T/R164S | 105 N-[5-METHYL-3-O-TOLYL-ISOXAZOLE-4-CARBOXYLIC ACID AMIDE] BORONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.80 Å R-free 0.189 |
| 1LHY Crystal structure of TEM-30 beta-Lactamase at 2.0 Angstrom Deposited 2002-04-17 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：R241S | PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 2.00 Å R-free 0.212 |
| 1LI0 Crystal structure of TEM-32 beta-Lactamase at 1.6 Angstrom Deposited 2002-04-17 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M69I, M182T | BCT BICARBONATE ION × 1 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.61 Å R-free 0.217 |
| 1LI9 Crystal structure of TEM-34 beta-Lactamase at 1.5 Angstrom Deposited 2002-04-17 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M69V | PO4 PHOSPHATE ION × 2 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.52 Å R-free 0.189 |
| 1M40 ULTRA HIGH RESOLUTION CRYSTAL STRUCTURE OF TEM-1 Deposited 2002-07-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T | PO4 PHOSPHATE ION × 3 K POTASSIUM ION × 4 CB4 PINACOL[[2-AMINO-ALPHA-(1-CARBOXY-1-METHYLETHOXYIMINO)-4-THIAZOLEACETYL]AMINO]METHANEBORONATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium buffer, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 0.85 Å R-free 0.112 |
| 1NXY Crystal Structure of the complex between M182T mutant of TEM-1 and a boronic acid inhibitor (SM2) Deposited 2003-02-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T | K POTASSIUM ION × 1 SM2 (1R)-1-(2-THIENYLACETYLAMINO)-1-(3-CARBOXYPHENYL)METHYLBORONIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.3；295 K；sodium-potassium buffer, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.60 Å R-free 0.196 |
| 1NY0 Crystal Structure of the complex between M182T mutant of TEM-1 and a boronic acid inhibitor (NBF) Deposited 2003-02-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T | PO4 PHOSPHATE ION × 1 K POTASSIUM ION × 1 NBF [(2-ETHOXY-1-NAPHTHOYL)AMINO]METHYLBORONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.3；295 K；sodium-potassium buffer , pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.75 Å R-free 0.198 |
| 1NYM Crystal Structure of the complex between M182T mutant of TEM-1 and a boronic acid inhibitor (CXB) Deposited 2003-02-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T | K POTASSIUM ION × 3 PO4 PHOSPHATE ION × 2 CXB [(2-AMINO-ALPHA-METHOXYIMINO-4-THIAZOLYLACETYL)AMINO]METHYLBORONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.3；295 K；sodium-potassium buffer, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.20 Å R-free 0.148 |
| 1NYY Crystal Structure of the complex between M182T mutant of TEM-1 and a boronic acid inhibitor (105) Deposited 2003-02-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T | 105 N-[5-METHYL-3-O-TOLYL-ISOXAZOLE-4-CARBOXYLIC ACID AMIDE] BORONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.3；295 K；sodium-potassium buffer , pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.90 Å R-free 0.230 |
| 1PZO TEM-1 Beta-Lactamase in Complex with a Novel, Core-Disrupting, Allosteric Inhibitor Deposited 2003-07-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T | CBT N,N-BIS(4-CHLOROBENZYL)-1H-1,2,3,4-TETRAAZOL-5-AMINE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.3；295 K；potassium phosphate buffer, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.90 Å R-free 0.247 |
| 1PZP TEM-1 Beta-Lactamase in Complex with a Novel, Core-Disrupting, Allosteric Inhibitor Deposited 2003-07-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：R100N | FTA 3-(4-PHENYLAMINO-PHENYLAMINO)-2-(1H-TETRAZOL-5-YL)-ACRYLONITRILE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.3；295 K；potassium phosphate buffer, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.45 Å R-free 0.245 |
| 1TEM 6 ALPHA HYDROXYMETHYL PENICILLOIC ACID ACYLATED ON THE TEM-1 BETA-LACTAMASE FROM ESCHERICHIA COLI Deposited 1996-05-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Not recorded | ALP 2-(1-CARBOXY-2-HYDROXY-ETHYL)-5,5-DIMETHYL-THIAZOLIDINE-4-CARBOXYLIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.8；pH 7.8
|
Resolution 1.95 Å R-free 0.202 |
| 1XPB STRUCTURE OF BETA-LACTAMASE TEM1 Deposited 1997-01-10 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å R-free 0.158 |
| 1XXM The modular architecture of protein-protein binding site Deposited 2004-11-07 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
24–286（263 aa）
|
Mutation：E104A; Y105A | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 5；298 K；LiCl; sodium Acetate; PEG 6000, pH 5., Microbatch, temperature 298K
|
Resolution 1.90 Å R-free 0.247 |
| 1XXM The modular architecture of protein-protein binding site Deposited 2004-11-07 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
24–286（263 aa）
|
Mutation：E104A; Y105A | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 5；298 K；LiCl; sodium Acetate; PEG 6000, pH 5., Microbatch, temperature 298K
|
Resolution 1.90 Å R-free 0.247 |
| 1YT4 Crystal structure of TEM-76 beta-lactamase at 1.4 Angstrom resolution Deposited 2005-02-09 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM
|
Mutation：S130G | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.3；296 K；sodium potassium phosphate, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 1.40 Å R-free 0.223 |
| 1ZG4 TEM1 beta lactamase Deposited 2005-04-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–286（286 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.55 Å R-free 0.240 |
| 1ZG6 TEM1 beta lactamase mutant S70G Deposited 2005-04-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–286（286 aa）
|
Mutation：S70G | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.10 Å R-free 0.275 |
| 2B5R 1B Lactamase / B Lactamase Inhibitor Deposited 2005-09-29 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
24–286（263 aa）
|
Mutation：V84I, E104Y, Y105N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.5；298 K；PEG 3350, NH4 Acetate, pH 8.5, Microbatch, temperature 298K
|
Resolution 1.65 Å R-free 0.222 |
| 2B5R 1B Lactamase / B Lactamase Inhibitor Deposited 2005-09-29 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
24–286（263 aa）
|
Mutation：V84I, E104Y, Y105N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.5；298 K；PEG 3350, NH4 Acetate, pH 8.5, Microbatch, temperature 298K
|
Resolution 1.65 Å R-free 0.222 |
| 2V1Z Structure of a TEM-1 beta-lactamase insertant allosterically regulated by kanamycin and anions. Deposited 2007-05-31 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
25–38（14 aa）
Fragment：RESIDUES 25-38,41-286
Chain A
41–286（246 aa）
Fragment：RESIDUES 25-38,41-286
|
Mutation：YES Mutation：YES | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.2；RESERVOIR: BIS-TRIS 0.1M PH6.2, PEG6000 25%(W/V), NACL 0.3M, NAN3 0.02%(W/V). HANGING DROP: 1UL PROTEIN AND 1 UL RESERVOIR
|
Resolution 1.60 Å R-free 0.206 |
| 2V20 Structure of a TEM-1 beta-lactamase insertant allosterically regulated by kanamycin and anions. Complex with sulfate. Deposited 2007-05-31 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
25–38（14 aa）
Fragment：RESIDUES 25-38,41-286
Chain A
41–286（246 aa）
Fragment：RESIDUES 25-38,41-286
|
Mutation：YES Mutation：YES | ZN ZINC ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；RESERVOIR: MES 0.1M PH 6.5, PEG5000MME 30%(W/V), AMMONIUM SULFATE 0.2M. DROP: 0.5UL PROTEIN SOLUTION AND 0.5 UL RESERVOIR
|
Resolution 1.67 Å R-free 0.223 |
| 3CMZ TEM-1 Class-A beta-lactamase L201P mutant apo structure Deposited 2008-03-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：L201P | PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.7；293 K；1.6M potassium phosphate, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.92 Å R-free 0.242 |
| 3JYI Structural and biochemical evidence that a TEM-1 {beta}-lactamase Asn170Gly active site mutant acts via substrate-assisted catalysis Deposited 2009-09-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：N170G | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG6000, 0.2 M LiCl, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.260 |
| 3JYI Structural and biochemical evidence that a TEM-1 {beta}-lactamase Asn170Gly active site mutant acts via substrate-assisted catalysis Deposited 2009-09-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：N170G | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG6000, 0.2 M LiCl, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.260 |
| 3JYI Structural and biochemical evidence that a TEM-1 {beta}-lactamase Asn170Gly active site mutant acts via substrate-assisted catalysis Deposited 2009-09-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：N170G | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG6000, 0.2 M LiCl, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.260 |
| 3JYI Structural and biochemical evidence that a TEM-1 {beta}-lactamase Asn170Gly active site mutant acts via substrate-assisted catalysis Deposited 2009-09-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：N170G | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG6000, 0.2 M LiCl, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.260 |
| 3JYI Structural and biochemical evidence that a TEM-1 {beta}-lactamase Asn170Gly active site mutant acts via substrate-assisted catalysis Deposited 2009-09-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
24–286（263 aa）
|
Mutation：N170G | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG6000, 0.2 M LiCl, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.260 |
| 3JYI Structural and biochemical evidence that a TEM-1 {beta}-lactamase Asn170Gly active site mutant acts via substrate-assisted catalysis Deposited 2009-09-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
24–286（263 aa）
|
Mutation：N170G | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG6000, 0.2 M LiCl, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.260 |
| 4DXB 2.29A structure of the engineered MBP TEM-1 fusion protein RG13 in complex with zinc, P1 space group Deposited 2012-02-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
227–286（60 aa）
Fragment：SEE REMARK 999
Chain A
24–226（203 aa）
Fragment：SEE REMARK 999
|
Not recorded | ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；A 1.0 uL drop was prepared using 0.5 uL protein mixture (13.8 mg/mL RG13, 2.5 mM zinc chloride) and 0.5 uL reservoir solution (0.2 M ammonium acetate, 0.1 M Tris, pH 8.5-9.5, 15-30% PEG3350) and equilibrated over a 1 ml reservoir solution, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K
|
Resolution 2.29 Å R-free 0.292 |
| 4DXB 2.29A structure of the engineered MBP TEM-1 fusion protein RG13 in complex with zinc, P1 space group Deposited 2012-02-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
227–286（60 aa）
Fragment：SEE REMARK 999
Chain B
24–226（203 aa）
Fragment：SEE REMARK 999
|
Not recorded | ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；A 1.0 uL drop was prepared using 0.5 uL protein mixture (13.8 mg/mL RG13, 2.5 mM zinc chloride) and 0.5 uL reservoir solution (0.2 M ammonium acetate, 0.1 M Tris, pH 8.5-9.5, 15-30% PEG3350) and equilibrated over a 1 ml reservoir solution, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K
|
Resolution 2.29 Å R-free 0.292 |
| 4DXC Crystal structure of the engineered MBP TEM-1 fusion protein RG13, C2 space group Deposited 2012-02-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
227–286（60 aa）
Fragment：SEE REMARK 999
Chain A
24–226（203 aa）
Fragment：SEE REMARK 999
|
Not recorded | ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；A 1.0 uL drop was prepared using 0.5 uL protein mixture (13.8 mg/mL RG13, 2.5 mM zinc chloride) and 0.5 uL reservoir solution (0.2 M ammonium acetate, 0.1 M Tris, pH 8.5-9.5, 15-30% PEG3350) and equilibrated over a 1 ml reservoir solution, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K
|
Resolution 2.30 Å R-free 0.291 |
| 4GKU Crystal structure of beta lactamase in PET-15B Deposited 2012-08-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.5；293 K；0.2M magnesium chloride, 0.1M BIS-Tris, 25% PEG 3350, pH 6.5, VAPOR DIFFUSION, temperature 293K
|
Resolution 1.92 Å R-free 0.197 |
| 4IBR Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying G238S/E104K mutations Deposited 2012-12-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：A41G, N51A, R119G, M181T, L200A, T262M, G237S, E103K | CA CALCIUM ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；11% (wt/vol) polyethylene glycol (PEG) 8000, 100 mM
MES buffer pH 6.7, 200mM Ca(OAc)2 and 10 M ZnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.247 |
| 4IBR Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying G238S/E104K mutations Deposited 2012-12-09 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
24–286（263 aa）
|
Mutation：A41G, N51A, R119G, M181T, L200A, T262M, G237S, E103K | CA CALCIUM ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；11% (wt/vol) polyethylene glycol (PEG) 8000, 100 mM
MES buffer pH 6.7, 200mM Ca(OAc)2 and 10 M ZnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.247 |
| 4IBX Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 Deposited 2012-12-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：A42G, N52A, I84V, R120G, M182T, L201A, T265M | CA CALCIUM ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.2；293 K；9%(wt/vol) polyethylene glycol (PEG) 8000, 100 mM MES pH 6.2, and 200mM
Ca(OAc)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.68 Å R-free 0.265 |
| 4IBX Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 Deposited 2012-12-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：A42G, N52A, I84V, R120G, M182T, L201A, T265M | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.2；293 K；9%(wt/vol) polyethylene glycol (PEG) 8000, 100 mM MES pH 6.2, and 200mM
Ca(OAc)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.68 Å R-free 0.265 |
| 4IBX Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 Deposited 2012-12-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：A42G, N52A, I84V, R120G, M182T, L201A, T265M | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.2；293 K；9%(wt/vol) polyethylene glycol (PEG) 8000, 100 mM MES pH 6.2, and 200mM
Ca(OAc)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.68 Å R-free 0.265 |
| 4IBX Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 Deposited 2012-12-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：A42G, N52A, I84V, R120G, M182T, L201A, T265M | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.2；293 K；9%(wt/vol) polyethylene glycol (PEG) 8000, 100 mM MES pH 6.2, and 200mM
Ca(OAc)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.68 Å R-free 0.265 |
| 4IBX Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 Deposited 2012-12-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
24–286（263 aa）
|
Mutation：A42G, N52A, I84V, R120G, M182T, L201A, T265M | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.2；293 K；9%(wt/vol) polyethylene glycol (PEG) 8000, 100 mM MES pH 6.2, and 200mM
Ca(OAc)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.68 Å R-free 0.265 |
| 4ID4 Crystal structure of chimeric beta-lactamase cTEM-17m Deposited 2012-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–147（124 aa）
Chain A
189–286（98 aa）
|
Not recorded | CL CHLORIDE ION × 5 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；26% PEG 4000, 0.25M magnesium chloride, 0.1M TrisHCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.05 Å R-free 0.138 |
| 4MEZ Crystal structure of M68L/M69T double mutant TEM-1 Deposited 2013-08-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M66L, M67T | CL CHLORIDE ION × 1 SO4 SULFATE ION × 2 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295.15 K；1.5M ammonium sulfate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K
|
Resolution 2.05 Å R-free 0.248 |
| 4MEZ Crystal structure of M68L/M69T double mutant TEM-1 Deposited 2013-08-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M66L, M67T | CL CHLORIDE ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295.15 K；1.5M ammonium sulfate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K
|
Resolution 2.05 Å R-free 0.248 |
| 4QY5 Crystal structures of chimeric beta-lactamase cTEM-19m showing different conformations Deposited 2014-07-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–65（42 aa）
Chain A
68–147（80 aa）
Chain A
189–286（98 aa）
|
Not recorded | CL CHLORIDE ION × 4 MG MAGNESIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；30% PEG 4000, 0.2M magnesium chloride, 0.1M TrisHCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
Resolution 1.50 Å R-free 0.161 |
| 4QY6 Crystal structures of chimeric beta-lactamase cTEM-19m showing different conformations Deposited 2014-07-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–65（42 aa）
Chain A
68–147（80 aa）
Chain A
189–286（98 aa）
|
Not recorded | CL CHLORIDE ION × 6 MG MAGNESIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；30% PEG 4000, 0.2M magnesium chloride, 0.1M TrisHCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.15 Å R-free 0.139 |
| 4R4R Crystal structure of chimeric beta-lactamase cTEM-19m at 1.2 angstrom resolution Deposited 2014-08-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–65（42 aa）
Chain A
68–147（80 aa）
Chain A
189–286（98 aa）
|
Not recorded | CL CHLORIDE ION × 4 MG MAGNESIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；30% PEG 4000, 0.2M magnesium chloride, 0.1M TrisHCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.20 Å R-free 0.138 |
| 4R4S Crystal structure of chimeric beta-lactamase cTEM-19m at 1.1 angstrom resolution Deposited 2014-08-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–65（42 aa）
Chain A
68–147（80 aa）
Chain A
189–286（98 aa）
|
Not recorded | CL CHLORIDE ION × 5 MG MAGNESIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；30% PEG 4000, 0.2M magnesium chloride, 0.1M TrisHCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.10 Å R-free 0.128 |
| 4RVA A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for deacylation Deposited 2014-11-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM-1
|
Mutation：W165Y/E166Y/P167G/L201P | BCT BICARBONATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；20% PEG 3350, 0.2M sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.44 Å R-free 0.194 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain G
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain H
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX3 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：S70G/W165Y/E166Y/P167G | FLC CITRATE ANION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；0.24M citrate, 25% w/v PEG 4,000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.39 Å R-free 0.194 |
| 4ZJ1 Crystal Structure of p-acrylamido-phenylalanine modified TEM1 beta-lactamase from Escherichia coli : V216AcrF mutant Deposited 2015-04-28 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–286（286 aa）
|
Mutation：V216AcrF Non-standard monomer：Yes (specific site not provided by mmCIF) | GOL GLYCEROL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 PEG DI(HYDROXYETHYL)ETHER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.8；295 K；0.1M 2-(N-morpholino) ethanesulfonic acid (MES) pH 6.5, 15% PEG 20.000 and 15% PEG 550MME
|
Resolution 1.54 Å R-free 0.170 |
| 4ZJ2 Crystal Structure of p-acrylamido-phenylalanine modified TEM1 beta-lactamase from Escherichia coli :E166N mutant Deposited 2015-04-28 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–286（286 aa）
|
Mutation：E166N Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.8；295 K；0.1M 2-(N-morpholino) ethanesulfonic acid (MES) pH 6.5, 15% PEG 20.000 and 15% PEG 550MME
|
Resolution 1.80 Å R-free 0.209 |
| 4ZJ3 Crystal structure of cephalexin bound acyl-enzyme intermediate of Val216AcrF mutant TEM1 beta-lactamase from Escherichia coli: E166N and V216AcrF mutant. Deposited 2015-04-29 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–286（286 aa）
|
Mutation：E166N,V216AcrF Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.8；295 K；0.1M 2-(N-morpholino) ethanesulfonic acid (MES) pH 6.5, 15% PEG 20.000 and 15% PEG 550MME
|
Resolution 1.70 Å R-free 0.188 |
| 5HVI Crystal structure of TEM1 beta-lactamase Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M180T | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.64 Å R-free 0.206 |
| 5HVI Crystal structure of TEM1 beta-lactamase Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M180T | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.64 Å R-free 0.206 |
| 5HVI Crystal structure of TEM1 beta-lactamase Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M180T | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.64 Å R-free 0.206 |
| 5HVI Crystal structure of TEM1 beta-lactamase Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M180T | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.64 Å R-free 0.206 |
| 5HW1 Crystal structure of TEM1 beta-lactamase in the presence of 1.2 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M180T | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.70 Å R-free 0.190 |
| 5HW1 Crystal structure of TEM1 beta-lactamase in the presence of 1.2 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M180T | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.70 Å R-free 0.190 |
| 5HW1 Crystal structure of TEM1 beta-lactamase in the presence of 1.2 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M180T | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.70 Å R-free 0.190 |
| 5HW1 Crystal structure of TEM1 beta-lactamase in the presence of 1.2 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M180T | XE XENON × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.70 Å R-free 0.190 |
| 5HW5 Crystal structure of TEM1 beta-lactamase in the presence of 2.0 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M180T | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.41 Å R-free 0.221 |
| 5HW5 Crystal structure of TEM1 beta-lactamase in the presence of 2.0 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M180T | XE XENON × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.41 Å R-free 0.221 |
| 5HW5 Crystal structure of TEM1 beta-lactamase in the presence of 2.0 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M180T | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.41 Å R-free 0.221 |
| 5HW5 Crystal structure of TEM1 beta-lactamase in the presence of 2.0 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M180T | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.41 Å R-free 0.221 |
| 5I52 Crystal structure of TEM1 beta-lactamase mutant I263N Deposited 2016-02-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M180T, I259N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.75 Å R-free 0.218 |
| 5I52 Crystal structure of TEM1 beta-lactamase mutant I263N Deposited 2016-02-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M180T, I259N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.75 Å R-free 0.218 |
| 5I52 Crystal structure of TEM1 beta-lactamase mutant I263N Deposited 2016-02-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M180T, I259N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.75 Å R-free 0.218 |
| 5I52 Crystal structure of TEM1 beta-lactamase mutant I263N Deposited 2016-02-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M180T, I259N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.75 Å R-free 0.218 |
| 5I63 Crystal structure of TEM1 beta-lactamase mutant I263N in the presence of 1.2 MPa xenon Deposited 2016-02-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M180T, I259N | XE XENON × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.95 Å R-free 0.222 |
| 5I63 Crystal structure of TEM1 beta-lactamase mutant I263N in the presence of 1.2 MPa xenon Deposited 2016-02-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M180T, I259N | XE XENON × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.95 Å R-free 0.222 |
| 5I63 Crystal structure of TEM1 beta-lactamase mutant I263N in the presence of 1.2 MPa xenon Deposited 2016-02-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M180T, I259N | XE XENON × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.95 Å R-free 0.222 |
| 5I63 Crystal structure of TEM1 beta-lactamase mutant I263N in the presence of 1.2 MPa xenon Deposited 2016-02-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M180T, I259N | XE XENON × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.95 Å R-free 0.222 |
| 5IQ8 Crystal structure of TEM1 beta-lactamase mutant A224C/G283C disulfide Deposited 2016-03-10 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M180T, A222C, G279C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M ammonium tartrate dibasic (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 2.06 Å R-free 0.241 |
| 5IQ8 Crystal structure of TEM1 beta-lactamase mutant A224C/G283C disulfide Deposited 2016-03-10 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M180T, A222C, G279C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M ammonium tartrate dibasic (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 2.06 Å R-free 0.241 |
| 5IQ8 Crystal structure of TEM1 beta-lactamase mutant A224C/G283C disulfide Deposited 2016-03-10 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M180T, A222C, G279C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M ammonium tartrate dibasic (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 2.06 Å R-free 0.241 |
| 5IQ8 Crystal structure of TEM1 beta-lactamase mutant A224C/G283C disulfide Deposited 2016-03-10 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M180T, A222C, G279C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M ammonium tartrate dibasic (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 2.06 Å R-free 0.241 |
| 5KKF Crystal structure of TEM1 beta-lactamase mutant I263L Deposited 2016-06-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M180T, I259L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.82 Å R-free 0.227 |
| 5KKF Crystal structure of TEM1 beta-lactamase mutant I263L Deposited 2016-06-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M180T, I259L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.82 Å R-free 0.227 |
| 5KKF Crystal structure of TEM1 beta-lactamase mutant I263L Deposited 2016-06-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M180T, I259L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.82 Å R-free 0.227 |
| 5KKF Crystal structure of TEM1 beta-lactamase mutant I263L Deposited 2016-06-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M180T, I259L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.82 Å R-free 0.227 |
| 5KPU Crystal structure of TEM1 beta-lactamase mutant I263L in the presence of 1.2 MPa xenon Deposited 2016-07-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T, I263L | XE XENON × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.50 Å R-free 0.193 |
| 5KPU Crystal structure of TEM1 beta-lactamase mutant I263L in the presence of 1.2 MPa xenon Deposited 2016-07-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M182T, I263L | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.50 Å R-free 0.193 |
| 5KPU Crystal structure of TEM1 beta-lactamase mutant I263L in the presence of 1.2 MPa xenon Deposited 2016-07-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M182T, I263L | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.50 Å R-free 0.193 |
| 5KPU Crystal structure of TEM1 beta-lactamase mutant I263L in the presence of 1.2 MPa xenon Deposited 2016-07-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M182T, I263L | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.50 Å R-free 0.193 |
| 5NPO Promiscuous Protein Self-Assembly as a Function of Protein Stability Deposited 2017-04-18 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
24–286（263 aa）
|
Not recorded | MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；292 K；7.5% PEG 6000, 0.1M MgCl2 and 0.05M Sodium acetate pH=5.5
|
Resolution 1.95 Å R-free 0.256 |
| 6APA Crystal structure of TEM1 beta-lactamase mutant I263A Deposited 2017-08-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.86 Å R-free 0.249 |
| 6APA Crystal structure of TEM1 beta-lactamase mutant I263A Deposited 2017-08-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.86 Å R-free 0.249 |
| 6APA Crystal structure of TEM1 beta-lactamase mutant I263A Deposited 2017-08-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.86 Å R-free 0.249 |
| 6APA Crystal structure of TEM1 beta-lactamase mutant I263A Deposited 2017-08-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.86 Å R-free 0.249 |
| 6AYK Crystal structure of TEM1 beta-lactamase mutant I263A in the presence of 1.2 MPa xenon Deposited 2017-09-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v Tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.44 Å R-free 0.219 |
| 6AYK Crystal structure of TEM1 beta-lactamase mutant I263A in the presence of 1.2 MPa xenon Deposited 2017-09-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v Tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.44 Å R-free 0.219 |
| 6AYK Crystal structure of TEM1 beta-lactamase mutant I263A in the presence of 1.2 MPa xenon Deposited 2017-09-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v Tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.44 Å R-free 0.219 |
| 6AYK Crystal structure of TEM1 beta-lactamase mutant I263A in the presence of 1.2 MPa xenon Deposited 2017-09-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | XE XENON × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v Tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.44 Å R-free 0.219 |
| 6B2N Crystal structure of TEM-1 beta-lactamase mutant M182N Deposited 2017-09-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.2；291 K；0.1 M sodium phosphate dibasic/citric acid, pH 4.2, 0.1 M lithium sulfate, 20% PEG1000
|
Resolution 2.00 Å R-free 0.283 |
| 6B2N Crystal structure of TEM-1 beta-lactamase mutant M182N Deposited 2017-09-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182N | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.2；291 K；0.1 M sodium phosphate dibasic/citric acid, pH 4.2, 0.1 M lithium sulfate, 20% PEG1000
|
Resolution 2.00 Å R-free 0.283 |
| 6B2N Crystal structure of TEM-1 beta-lactamase mutant M182N Deposited 2017-09-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182N | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.2；291 K；0.1 M sodium phosphate dibasic/citric acid, pH 4.2, 0.1 M lithium sulfate, 20% PEG1000
|
Resolution 2.00 Å R-free 0.283 |
| 6B2N Crystal structure of TEM-1 beta-lactamase mutant M182N Deposited 2017-09-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182N | SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.2；291 K；0.1 M sodium phosphate dibasic/citric acid, pH 4.2, 0.1 M lithium sulfate, 20% PEG1000
|
Resolution 2.00 Å R-free 0.283 |
| 7QLP Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.3 A resolution Deposited 2021-12-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 ACT ACETATE ION × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.30 Å R-free 0.217 |
| 7QLP Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.3 A resolution Deposited 2021-12-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：V84I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.30 Å R-free 0.217 |
| 7QLP Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.3 A resolution Deposited 2021-12-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.30 Å R-free 0.217 |
| 7QLP Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.3 A resolution Deposited 2021-12-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.30 Å R-free 0.217 |
| 7QLP Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.3 A resolution Deposited 2021-12-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.30 Å R-free 0.217 |
| 7QLP Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.3 A resolution Deposited 2021-12-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
24–286（263 aa）
|
Mutation：V84I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.30 Å R-free 0.217 |
| 7QNK Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.5 A resolution Deposited 2021-12-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.50 Å R-free 0.248 |
| 7QNK Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.5 A resolution Deposited 2021-12-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.50 Å R-free 0.248 |
| 7QNK Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.5 A resolution Deposited 2021-12-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.50 Å R-free 0.248 |
| 7QNK Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.5 A resolution Deposited 2021-12-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.50 Å R-free 0.248 |
| 7QNK Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.5 A resolution Deposited 2021-12-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.50 Å R-free 0.248 |
| 7QNK Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.5 A resolution Deposited 2021-12-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
24–286（263 aa）
|
Mutation：V84I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.50 Å R-free 0.248 |
| 7QOR Structure of beta-lactamase TEM-171 Deposited 2021-12-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain AAA
24–286（263 aa）
|
Mutation：V84I | EDO 1,2-ETHANEDIOL × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.211 |
| 7QOR Structure of beta-lactamase TEM-171 Deposited 2021-12-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain BBB
24–286（263 aa）
|
Mutation：V84I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.211 |
| 7QOR Structure of beta-lactamase TEM-171 Deposited 2021-12-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain CCC
24–286（263 aa）
|
Mutation：V84I | EDO 1,2-ETHANEDIOL × 2 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.211 |
| 7QOR Structure of beta-lactamase TEM-171 Deposited 2021-12-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain DDD
24–286（263 aa）
|
Mutation：V84I | ACT ACETATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.211 |
| 7QOR Structure of beta-lactamase TEM-171 Deposited 2021-12-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain EEE
24–286（263 aa）
|
Mutation：V84I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.211 |
| 7QOR Structure of beta-lactamase TEM-171 Deposited 2021-12-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain FFF
24–286（263 aa）
|
Mutation：V84I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.211 |
| 8DDZ TEM-1 beta-lactamase A237Y Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T, A237Y | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG 3350, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)
|
Resolution 1.45 Å R-free 0.212 |
| 8DDZ TEM-1 beta-lactamase A237Y Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M182T, A237Y | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG 3350, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)
|
Resolution 1.45 Å R-free 0.212 |
| 8DDZ TEM-1 beta-lactamase A237Y Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M182T, A237Y | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG 3350, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)
|
Resolution 1.45 Å R-free 0.212 |
| 8DDZ TEM-1 beta-lactamase A237Y Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M182T, A237Y | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG 3350, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)
|
Resolution 1.45 Å R-free 0.212 |
| 8DE0 TEM-1 beta-lactamase covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.72 Å R-free 0.198 |
| 8DE0 TEM-1 beta-lactamase covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M182T | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.72 Å R-free 0.198 |
| 8DE0 TEM-1 beta-lactamase covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M182T | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.72 Å R-free 0.198 |
| 8DE0 TEM-1 beta-lactamase covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M182T | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.72 Å R-free 0.198 |
| 8DE1 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.56 Å R-free 0.221 |
| 8DE1 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M182T A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.56 Å R-free 0.221 |
| 8DE1 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M182T A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.56 Å R-free 0.221 |
| 8DE1 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M182T A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.56 Å R-free 0.221 |
| 8DE2 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam, a room temperature structure Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T, A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES(4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 2.45 Å R-free 0.249 |
| 8DE2 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam, a room temperature structure Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M182T, A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES(4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 2.45 Å R-free 0.249 |
| 8DE2 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam, a room temperature structure Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M182T, A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES(4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 2.45 Å R-free 0.249 |
| 8DE2 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam, a room temperature structure Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M182T, A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES(4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 2.45 Å R-free 0.249 |
| 9Q0C TEM-1 WT in complex with BLIP E73W Deposited 2025-08-12 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
24–286（263 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.1 M magnesium chloride, 0.1 M sodium acetate pH 4.6, 25% (w/v) PEG 400
|
Resolution 1.78 Å R-free 0.215 |
| 9Q0C TEM-1 WT in complex with BLIP E73W Deposited 2025-08-12 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
24–286（263 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.1 M magnesium chloride, 0.1 M sodium acetate pH 4.6, 25% (w/v) PEG 400
|
Resolution 1.78 Å R-free 0.215 |
72 other PDB entries and 155 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | BLAT_ECOLI |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–263； UniProt 24–286 |
