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1axk

ENGINEERED BACILLUS BIFUNCTIONAL ENZYME GLUXYN-1

Method: X-RAY DIFFRACTION D_max: 111.2 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1axk
deposition_date	1997-10-16
title	ENGINEERED BACILLUS BIFUNCTIONAL ENZYME GLUXYN-1
keywords	GLUXYN-1, BIFUNCTIONAL, FUSION PROTEIN, 1, 4-BETA-XYLANASE, 3-1, 4-BETA-GLUCANASE, HYBRID ENZYME; FUSION PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	34.84 Å
rg_electron	34.19 Å
i0	122102000.00
molecular_weight	87548.0 kDa
excluded_volume	108550 Å³
envelope_volume	138300 Å³
shell_volume	34371 Å³
envelope_diameter	112.1 Å
shell_rg	40.01 Å
envelope_rg	33.41 Å
shape_rg	34.15 Å
total_rg	34.73 Å
total_atoms	6212
n_residues	787
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	111.2 Å
rg_real	34.82 Å
rg_real_error	0.90 Å
i0_real	1.2210e+08
i0_real_error	1.9890e+06
rg_reciprocal	34.84 Å
i0_reciprocal	122100000.0000
total_estimate	0.6699
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	49.4 Å
skewness	0.178
kurtosis	-0.658
angular_range	— – 0.2250 Å⁻¹
current_alpha	0.0000
highest_alpha	42130000.0000
n_real_points	46
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.896; Stabil: 1.000; Sysdev: 0.062; Positv: 1.000; Valcen: 0.960; Smooth: 0.871

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	GLUXYN-1
formula_weight	43812.7 kDa
ec_number	3.2.1.73, 3.2.1.8
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	6533

FDCAEYRSTNIYGYGLYEVSMKPAKNTGIVSSFFTYTGPAHGTQWDEIDIEFLGKDTTKVQFNYYTNGVGGHEKVISLGFDASKGFHTYAFDWQPGYIKWYVDGVLKHTATANIPSTPGKIMMNLWNGTGVDDWLGSYNGANPLYAEYDWVKYTSNASTDYWQNWTDGGGIVNAVNGSGGNYSVNWSNTGNFVVGKGWTTGSPFRTINYNAGVWAPNGNGYLTLYGWTRSPLIEYYVVDSWGTYRPTGTYKGTVKSDGGTYDIYTTTRYNAPSIDGDRTTFTQYWSVRQSKRPTGSNATITFSNHVNAWKSHGMNLGSNWAYQVMATEGYQSSGSSNVTVWGSVFWEPKSYFNPSTWEKADGYSNGGVFNCTWRANNVNFTNDGKLKLGLTSSA

Source

organism	Bacillus subtilis
ncbi_taxonomy_id	1423
host_organism	Escherichia coli

Entity 2 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	2

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	312

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6. Citations (1)

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length_a	45.270 Å
length_b	133.700 Å
length_c	77.950 Å
angle_alpha	90.00 °
angle_beta	99.76 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	2.100 Å
resolution_low	19.960 Å
r_factor	0.1770
r_work	0.1760
r_free	0.2240

fit	/app/data/saxs_computation/ax/1axk/1axk.fit
pddf	/app/data/pr_computation/ax/1axk/1axk.pr.out
profile	/app/data/saxs_computation/ax/1axk/1axk.dat

ENGINEERED BACILLUS BIFUNCTIONAL ENZYME GLUXYN-1

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction