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1bhy

LOW TEMPERATURE MIDDLE RESOLUTION STRUCTURE OF P64K FROM MASC DATA

Method: X-RAY DIFFRACTION Dmax: 84.7 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

P64K

OrganismNot specified

UniProt Q51225

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 112–593 Not recorded FAD FLAVIN-ADENINE DINUCLEOTIDE × 2 X-RAY DIFFRACTION X-ray crystallization conditions：PROTEIN WAS CRYSTALLIZED FROM AMMONIUM SULPHATE Resolution 4.18 Å R-free 0.323

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1BHY Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1OJT STRUCTURE OF DIHYDROLIPOAMIDE DEHYDROGENASE Deposited 1996-09-06 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 112–593（482 aa） Fragment：DIHYDROLIPOAMIDE DEHYDROGENASE DOMAIN
Not recorded FAD FLAVIN-ADENINE DINUCLEOTIDE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；BRIGHT YELLOW CRYSTALS WERE GROWN IN 24-WELL LINBRO PLATES USING HANGING DROP DIFFUSION METHOD AT ROOM TEMPERATURE. RESERVOIR: 1 ML CONTAINING 0.1M POTASSIUM PHOSPHATE AND 2M AMMONIUM SULFATE (PH 7.0). PRISM CRYSTALS TYPICALLY IN TWO WEEKS AT ROOM TEMPERATURE., vapor diffusion - hanging drop THE E3 DOMAIN (117-601) CRYSTALLIZES IN AMMONIUM SULFATE. IT INCLUDES A FAD COFACTOR BUT NOT THE NADH. THE E3 DOMAIN (117-601) CRYSTALLIZES IN AMMONIUM SULFATE. IT INCLUDE A FAD COFACTOR BUT NOT THE NADH.
Resolution 2.75 Å R-free 0.232

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q51225_NEIME
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–482； UniProt 112–593

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1bhy

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1bhy
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2. Structure Basics 2. Structure Basics

Entry ID entry_id1bhy
Deposition date deposition_date1998-06-10
Structure title titleLOW TEMPERATURE MIDDLE RESOLUTION STRUCTURE OF P64K FROM MASC DATA
Keywords keywordsOUTER MEMBRANE PROTEIN, MASC, MULTIWAVELENGTH ANOMALOUS SOLVENT CONTRAST; OUTER MEMBRANE PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier24.98 Å
Radius of gyration Rg (electron density) rg_electron24.03 Å
Forward intensity I(0) i044925500.00
Molecular weight molecular_weight51513.0 kDa
Excluded volume excluded_volume64383 ų
Envelope volume envelope_volume77560 ų
Hydration-shell volume shell_volume26781 ų
Envelope diameter envelope_diameter87.8 Å
Shell Rg shell_rg31.21 Å
Envelope Rg envelope_rg24.15 Å
Shape Rg shape_rg24.03 Å
Total Rg total_rg24.83 Å
Total atoms total_atoms3616
Residues n_residues482
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax84.7 Å
Rg (real space) rg_real24.88 Å
Rg uncertainty (real space) rg_real_error0.78 Å
I(0) (real space) i0_real4.4930e+07
I(0) uncertainty (real space) i0_real_error6.6090e+05
Rg (reciprocal space) rg_reciprocal24.91 Å
I(0) (reciprocal space) i0_reciprocal44930000.0000
Solution quality estimate total_estimate0.8074
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks3
Primary peak position r_peak_primary29.6 Å
Skewness Skewness skewness0.158
Kurtosis Kurtosis kurtosis-0.562
Angular range angular_range— – 0.3200 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha8969000.0000
Real-space data points n_real_points64
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.841; Stabil: 0.998; Sysdev: 1.000; Positv: 1.000; Valcen: 0.976; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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7. Fold Classification (SCOP + CATH) 3 domains

SCOP 2.08 (3 domains)

Domain ID domain_idd1bhya1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.3 — FAD/NAD(P)-binding domain
Superfamily Superfamily superfamilyc.3.1 — FAD/NAD(P)-binding domain
Family Family familyc.3.1.5 — FAD/NAD-linked reductases, N-terminal and central domains
Domain ID domain_idd1bhya2
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.3 — FAD/NAD(P)-binding domain
Superfamily Superfamily superfamilyc.3.1 — FAD/NAD(P)-binding domain
Family Family familyc.3.1.5 — FAD/NAD-linked reductases, N-terminal and central domains
Domain ID domain_idd1bhya3
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.87 — CO dehydrogenase flavoprotein C-domain-like
Superfamily Superfamily superfamilyd.87.1 — FAD/NAD-linked reductases, dimerisation (C-terminal) domain
Family Family familyd.87.1.1 — FAD/NAD-linked reductases, dimerisation (C-terminal) domain
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8. Citations (2)

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9. Files and Curves (10)