1bkh
MUCONATE LACTONIZING ENZYME FROM PSEUDOMONAS PUTIDA
1. 蛋白身份与相关结构 Protein Identity & Related Structures
当前条目没有可用的 UniProt 蛋白身份。
七张关系表仍保留该条目的 assembly 与组成信息，但缺少统一蛋白身份时，不能可靠建立跨 PDB 的同蛋白链接。
当前条目的 assembly 组成
| Assembly | 聚集状态 | 实体与构建体证据 | 突变与修饰 | 配体、离子与共同组分 | 实验方法与环境 | 结构质量 |
|---|---|---|---|---|---|---|
| 1 | 蛋白同源多聚体 同源多聚体 蛋白 8 / DNA 0 / RNA 0 / 其他聚合物 0 PDB 声明：octameric | 实体 1：MUCONATE LACTONIZING ENZYME × 8 缺少 UniProt 身份时不显示参考序列区间 | 未记录 | 未记录非水小分子 | X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, SITTING DROP；pH 5.2；MUCONATE LACTONIZING ENZYME (MLE; 5-10 MG/ML) WAS DIALYZED AGAINST 0.2 MM MNCL2, 7 MM MERCAPTOETHANOL, 50 MM TRIS (PH 7), AND CIS, CIS-MUCONATE WAS ADDED TO A FINAL CONCENTRATION OF 0.2 MM. CRYSTALS WERE GROWN AT ROOM TEMPERATURE BY SITTING-DROP VAPOR DIFFUSION AGAINST A WELL SOLUTION OF 70 MM NACL, 70 MM SODIUM ACETATE (PH 5.2), 0.25% POLYETHYLENE GLYCOL (AVERAGE MOLECULAR WEIGHT 3350). DROPS CONTAINED EQUAL VOLUMES (10 MICRO L) OF WELL SOLUTION AND MLE., vapor diffusion - sitting drop | 分辨率 2.10 Å |
| 2 | 蛋白同源多聚体 同源多聚体 蛋白 8 / DNA 0 / RNA 0 / 其他聚合物 0 PDB 声明：octameric | 实体 1：MUCONATE LACTONIZING ENZYME × 8 缺少 UniProt 身份时不显示参考序列区间 | 未记录 | 未记录非水小分子 | X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, SITTING DROP；pH 5.2；MUCONATE LACTONIZING ENZYME (MLE; 5-10 MG/ML) WAS DIALYZED AGAINST 0.2 MM MNCL2, 7 MM MERCAPTOETHANOL, 50 MM TRIS (PH 7), AND CIS, CIS-MUCONATE WAS ADDED TO A FINAL CONCENTRATION OF 0.2 MM. CRYSTALS WERE GROWN AT ROOM TEMPERATURE BY SITTING-DROP VAPOR DIFFUSION AGAINST A WELL SOLUTION OF 70 MM NACL, 70 MM SODIUM ACETATE (PH 5.2), 0.25% POLYETHYLENE GLYCOL (AVERAGE MOLECULAR WEIGHT 3350). DROPS CONTAINED EQUAL VOLUMES (10 MICRO L) OF WELL SOLUTION AND MLE., vapor diffusion - sitting drop | 分辨率 2.10 Å |
页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。 链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。
SAXS 散射曲线 SAXS Profile
P(r) 距离分布 P(r) Distribution
2. 结构基本信息 2. Structure Basics
| 条目编号 entry_id | 1bkh |
| 沉积日期 deposition_date | 1998-07-07 |
| 结构标题 title | MUCONATE LACTONIZING ENZYME FROM PSEUDOMONAS PUTIDA |
| 关键词 keywords | MUCONATE LACTONIZING ENZYME, MUCONATE CYCLOISOMERASE AROMATIC HYDROCARBONS CATABOLISM, ISOMERASE, MUCONATE CYCLOISOMERASE; MUCONATE CYCLOISOMERASE |
| 实验方法 method | X-RAY DIFFRACTION |
3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)
| 回转半径 Rg (Guinier) rg_guinier | 41.17 Å |
| 回转半径 Rg (电子) rg_electron | 41.43 Å |
| 零角强度 I(0) i0 | 202269000.00 |
| 分子量 molecular_weight | 115850.0 kDa |
| 排除体积 excluded_volume | 145350 ų |
| 包络体积 envelope_volume | 188080 ų |
| 水化壳体积 shell_volume | 40354 ų |
| 包络直径 envelope_diameter | 144.6 Å |
| 壳层 Rg shell_rg | 42.95 Å |
| 包络 Rg envelope_rg | 41.08 Å |
| 形状 Rg shape_rg | 41.41 Å |
| 总 Rg total_rg | 41.56 Å |
| 总原子数 total_atoms | 8157 |
| 残基数 n_residues | 1078 |
| 球谐函数阶数 n_harmonics | 20 |
| q 范围 q_range | — – 0.5000 Å−1 |
| 数据点数 n_points | 101 |
| 壳层类型 shell_type | directional |
| 溶剂电子密度 solvent_density | 0.3340 e/ų |
| 壳层衬度 contrast_shell | 0.0300 e/ų |
| CRYSOL 版本 crysol_version | 4.1.3 |
4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)
| 最大尺寸 Dmax dmax | 137.2 Å |
| Rg (实空间) rg_real | 41.53 Å |
| Rg 误差 (实空间) rg_real_error | 1.09 Å |
| I(0) (实空间) i0_real | 2.0230e+08 |
| I(0) 误差 (实空间) i0_real_error | 3.3230e+06 |
| Rg (倒空间) rg_reciprocal | 41.17 Å |
| I(0) (倒空间) i0_reciprocal | 202200000.0000 |
| 解质量估计 total_estimate | 0.8013 |
| 解质量评级 solution_quality | GOOD a GOOD solution |
| P(r) 峰数 n_peaks | 1 |
| 主峰位置 r_peak_primary | 32.5 Å |
| 偏度 Skewness skewness | 0.498 |
| 峰度 Kurtosis kurtosis | -0.514 |
| 角度范围 angular_range | — – 0.1900 Å−1 |
| 当前正则化参数 α current_alpha | 0.0000 |
| 最高正则化参数 α highest_alpha | 29270000.0000 |
| 实空间数据点数 n_real_points | 39 |
| GNOM 版本 gnom_version | 4.1.3 |
| 质量判据 quality_criteria | AN1: 0.000; Oscil: 0.735; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.755; Smooth: 0.453 |
5. 晶体学与实验 5. Crystallography & Experiment
6. 实体与聚合物信息 Entities & Polymers (2)
7. 折叠分类 (SCOP + CATH) 12 domains
SCOP 2.08 (6 domains)
| 结构域编号 domain_id | d1bkha1 |
| 类 Class class | c — Alpha and beta proteins (a/b) |
| 折叠类型 Fold fold | c.1 — TIM beta/alpha-barrel |
| 超家族 Superfamily superfamily | c.1.11 — Enolase C-terminal domain-like |
| 家族 Family family | c.1.11.2 — D-glucarate dehydratase-like |
| 结构域编号 domain_id | d1bkha2 |
| 类 Class class | d — Alpha and beta proteins (a+b) |
| 折叠类型 Fold fold | d.54 — Enolase N-terminal domain-like |
| 超家族 Superfamily superfamily | d.54.1 — Enolase N-terminal domain-like |
| 家族 Family family | d.54.1.1 — Enolase N-terminal domain-like |
| 结构域编号 domain_id | d1bkhb1 |
| 类 Class class | c — Alpha and beta proteins (a/b) |
| 折叠类型 Fold fold | c.1 — TIM beta/alpha-barrel |
| 超家族 Superfamily superfamily | c.1.11 — Enolase C-terminal domain-like |
| 家族 Family family | c.1.11.2 — D-glucarate dehydratase-like |
| 结构域编号 domain_id | d1bkhb2 |
| 类 Class class | d — Alpha and beta proteins (a+b) |
| 折叠类型 Fold fold | d.54 — Enolase N-terminal domain-like |
| 超家族 Superfamily superfamily | d.54.1 — Enolase N-terminal domain-like |
| 家族 Family family | d.54.1.1 — Enolase N-terminal domain-like |
| 结构域编号 domain_id | d1bkhc1 |
| 类 Class class | c — Alpha and beta proteins (a/b) |
| 折叠类型 Fold fold | c.1 — TIM beta/alpha-barrel |
| 超家族 Superfamily superfamily | c.1.11 — Enolase C-terminal domain-like |
| 家族 Family family | c.1.11.2 — D-glucarate dehydratase-like |
| 结构域编号 domain_id | d1bkhc2 |
| 类 Class class | d — Alpha and beta proteins (a+b) |
| 折叠类型 Fold fold | d.54 — Enolase N-terminal domain-like |
| 超家族 Superfamily superfamily | d.54.1 — Enolase N-terminal domain-like |
| 家族 Family family | d.54.1.1 — Enolase N-terminal domain-like |
CATH v4.4 (6 domains)
| 结构域编号 domain_id | 1bkhA01 |
| 类 Class class | 3 — Alpha Beta |
| 架构 Architecture architecture | 30 — 2-Layer Sandwich |
| 拓扑 Topology topology | 390 — Enolase-like; domain 1 |
| 同源超家族 H-superfamily homologous superfamily | 10 — Enolase-like, N-terminal domain |
| 结构域编号 domain_id | 1bkhA02 |
| 类 Class class | 3 — Alpha Beta |
| 架构 Architecture architecture | 20 — Alpha-Beta Barrel |
| 拓扑 Topology topology | 20 — TIM Barrel |
| 同源超家族 H-superfamily homologous superfamily | 120 — Enolase-like C-terminal domain |
| 结构域编号 domain_id | 1bkhB01 |
| 类 Class class | 3 — Alpha Beta |
| 架构 Architecture architecture | 30 — 2-Layer Sandwich |
| 拓扑 Topology topology | 390 — Enolase-like; domain 1 |
| 同源超家族 H-superfamily homologous superfamily | 10 — Enolase-like, N-terminal domain |
| 结构域编号 domain_id | 1bkhB02 |
| 类 Class class | 3 — Alpha Beta |
| 架构 Architecture architecture | 20 — Alpha-Beta Barrel |
| 拓扑 Topology topology | 20 — TIM Barrel |
| 同源超家族 H-superfamily homologous superfamily | 120 — Enolase-like C-terminal domain |
| 结构域编号 domain_id | 1bkhC01 |
| 类 Class class | 3 — Alpha Beta |
| 架构 Architecture architecture | 30 — 2-Layer Sandwich |
| 拓扑 Topology topology | 390 — Enolase-like; domain 1 |
| 同源超家族 H-superfamily homologous superfamily | 10 — Enolase-like, N-terminal domain |
| 结构域编号 domain_id | 1bkhC02 |
| 类 Class class | 3 — Alpha Beta |
| 架构 Architecture architecture | 20 — Alpha-Beta Barrel |
| 拓扑 Topology topology | 20 — TIM Barrel |
| 同源超家族 H-superfamily homologous superfamily | 120 — Enolase-like C-terminal domain |