HOW A PROTEIN BINDS B12: A 3.O ANGSTROM X-RAY STRUCTURE OF THE B12-BINDING DOMAINS OF METHIONINE SYNTHASE

Method: X-RAY DIFFRACTION D_max: 74.2 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1bmt
deposition_date	1994-09-02
title	HOW A PROTEIN BINDS B12: A 3.O ANGSTROM X-RAY STRUCTURE OF THE B12-BINDING DOMAINS OF METHIONINE SYNTHASE
keywords	METHYLTRANSFERASE; METHYLTRANSFERASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	24.74 Å
rg_electron	23.64 Å
i0	53609700.00
molecular_weight	57035.0 kDa
excluded_volume	71588 Å³
envelope_volume	87753 Å³
shell_volume	30345 Å³
envelope_diameter	76.7 Å
shell_rg	31.35 Å
envelope_rg	23.31 Å
shape_rg	23.65 Å
total_rg	24.47 Å
total_atoms	3998
n_residues	492
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	74.2 Å
rg_real	24.52 Å
rg_real_error	0.35 Å
i0_real	5.3610e+07
i0_real_error	6.7900e+05
rg_reciprocal	24.57 Å
i0_reciprocal	53610000.0000
total_estimate	0.9056
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	34.2 Å
skewness	0.037
kurtosis	-0.509
angular_range	— – 0.3200 Å⁻¹
current_alpha	0.0000
highest_alpha	8438000.0000
n_real_points	64
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.931; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.981; Smooth: 0.994

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

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6. Citations (2)

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length_a	96.700 Å
length_b	55.300 Å
length_c	103.800 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	3.000 Å
resolution_low	8.000 Å
r_factor	0.1700
r_work	0.1700
r_free	—

description	METHIONINE SYNTHASE
formula_weight	27209.2 kDa
ec_number	2.1.1.13
src_method	man
entity_count	2

type	polypeptide(L)
strand_id	A,B
atom_count	4000

HOW A PROTEIN BINDS B12: A 3.O ANGSTROM X-RAY STRUCTURE OF THE B12-BINDING DOMAINS OF METHIONINE SYNTHASE

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 non-polymer

Entity 3 water

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	Escherichia coli

description	CO-METHYLCOBALAMIN
formula_weight	1344.4 kDa
src_method	syn
entity_count	2

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	2

fit	/app/data/saxs_computation/bm/1bmt/1bmt.fit
pddf	/app/data/pr_computation/bm/1bmt/1bmt.pr.out
profile	/app/data/saxs_computation/bm/1bmt/1bmt.dat