← Back to search

1c7d

DEOXY RHB1.2 (RECOMBINANT HEMOGLOBIN)

Method: X-RAY DIFFRACTION D_max: 69.8 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1c7d
deposition_date	2000-02-09
title	DEOXY RHB1.2 (RECOMBINANT HEMOGLOBIN)
keywords	HEME, OXYGEN DELIVERY VEHICLE, BLOOD SUBSTITUTE, OXYGEN STORAGE-TRANSPORT COMPLEX; OXYGEN STORAGE/TRANSPORT
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	24.89 Å
rg_electron	23.56 Å
i0	63834900.00
molecular_weight	64678.0 kDa
excluded_volume	81834 Å³
envelope_volume	97067 Å³
shell_volume	32994 Å³
envelope_diameter	70.7 Å
shell_rg	31.65 Å
envelope_rg	23.27 Å
shape_rg	23.55 Å
total_rg	24.48 Å
total_atoms	4568
n_residues	576
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	69.8 Å
rg_real	24.63 Å
rg_real_error	0.38 Å
i0_real	6.3830e+07
i0_real_error	8.2850e+05
rg_reciprocal	24.70 Å
i0_reciprocal	63840000.0000
total_estimate	0.9121
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	33.2 Å
skewness	-0.001
kurtosis	-0.575
angular_range	— – 0.3200 Å⁻¹
current_alpha	0.0000
highest_alpha	13580000.0000
n_real_points	64
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.976; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.968; Smooth: 0.963

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (4)

Entity 1 polymer

description	PROTEIN (DEOXYHEMOGLOBIN (ALPHA CHAIN))
formula_weight	30428.9 kDa
mutation	V1M,142G,143G
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	4812

MLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYRGGVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 2 polymer

description	PROTEIN (DEOXYHEMOGLOBIN (BETA CHAIN))
formula_weight	15937.3 kDa
mutation	V1M,N108K
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	B,D

MHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGKVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 3 non-polymer

description	PROTOPORPHYRIN IX CONTAINING FE
formula_weight	616.5 kDa
src_method	syn
entity_count	4

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	244

▶

6. Citations (3)

▶

length_a	63.144 Å
length_b	83.282 Å
length_c	53.928 Å
angle_alpha	90.00 °
angle_beta	99.07 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	1.800 Å
resolution_low	8.000 Å
r_factor	0.1879
r_work	—
r_free	0.2397

fit	/app/data/saxs_computation/c7/1c7d/1c7d.fit
pddf	/app/data/pr_computation/c7/1c7d/1c7d.pr.out
profile	/app/data/saxs_computation/c7/1c7d/1c7d.dat

DEOXY RHB1.2 (RECOMBINANT HEMOGLOBIN)

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 water

6. Citations (3)

7. Files & Curves (10)

Files (7)

Curves (3)