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1cbo

CHOLESTEROL OXIDASE FROM STREPTOMYCES HIS447ASN MUTANT

Method: X-RAY DIFFRACTION D_max: 76.9 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cbo
deposition_date	1999-02-26
title	CHOLESTEROL OXIDASE FROM STREPTOMYCES HIS447ASN MUTANT
keywords	FLAVOENZYME, STEROID METABOLISM, OXIDOREDUCTASE; FLAVOENZYME
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	22.98 Å
rg_electron	21.96 Å
i0	51232000.00
molecular_weight	54996.0 kDa
excluded_volume	68425 Å³
envelope_volume	77675 Å³
shell_volume	28545 Å³
envelope_diameter	80.2 Å
shell_rg	29.79 Å
envelope_rg	22.13 Å
shape_rg	21.96 Å
total_rg	22.80 Å
total_atoms	3876
n_residues	498
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	76.9 Å
rg_real	22.85 Å
rg_real_error	0.38 Å
i0_real	5.1230e+07
i0_real_error	6.5020e+05
rg_reciprocal	22.88 Å
i0_reciprocal	51230000.0000
total_estimate	0.7127
solution_quality	REASONABLE a REASONABLE solution
n_peaks	1
r_peak_primary	75.7 Å
skewness	0.206
kurtosis	-0.333
angular_range	— – 0.3450 Å⁻¹
current_alpha	0.0000
highest_alpha	13720000.0000
n_real_points	66
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.782; Stabil: 1.000; Sysdev: 0.307; Positv: 1.000; Valcen: 0.998; Smooth: 0.997

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	PROTEIN (CHOLESTEROL OXIDASE)
formula_weight	54945.6 kDa
ec_number	1.1.3.6
mutation	H447N
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	4185

DNGGYVPAVVIGTGYGAAVSALRLGEAGVQTLMLEMGQLWNQPGPDGNIFCGMLNPDKRSSWFKNRTEAPLGSFLWLDVVNRNIDPYAGVLDRVNYDQMSVYVGRGVGGGSLVNGGMAVEPKRSYFEEILPRVDSSEMYDRYFPRANSMLRVNHIDTKWFEDTEWYKFARVSREQAGKAGLGTVFVPNVYDFGYMQREAAGEVPKSALATEVIYGNNHGKQSLDKTYLAAALGTGKVTIQTLHQVKTIRQTKDGGYALTVEQKDTDGKLLATKEISCRYLFLGAGSLGSTELLVRARDTGTLPNLNSEVGAGWGPNGNIMTARANHMWNPTGAHQSSIPALGIDAWDNSDSSVFAEIAPMPAGLETWVSLYLAITKNPQRGTFVYDAATDRAKLNWTRDQNAPAVNAAKALFDRINKANGTIYRYDLFGTQLKAFADDFCYNPLGGCVLGKATDDYGRVAGYKNLYVTDGSLIPGSVGVNPFVTITALAERNVERIIKQDVTAS

Source

organism	Streptomyces sp.
ncbi_taxonomy_id	1931
host_organism	Escherichia coli

Entity 2 non-polymer

description	FLAVIN-ADENINE DINUCLEOTIDE
formula_weight	785.5 kDa
src_method	syn
entity_count	1

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	295

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6. Citations (1)

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length_a	51.580 Å
length_b	74.150 Å
length_c	63.740 Å
angle_alpha	90.00 °
angle_beta	105.08 °
angle_gamma	90.00 °
z_pdb	2

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	1.800 Å
resolution_low	20.000 Å
r_factor	—
r_work	0.1590
r_free	0.1940

fit	/app/data/saxs_computation/cb/1cbo/1cbo.fit
pddf	/app/data/pr_computation/cb/1cbo/1cbo.pr.out
profile	/app/data/saxs_computation/cb/1cbo/1cbo.dat

CHOLESTEROL OXIDASE FROM STREPTOMYCES HIS447ASN MUTANT

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction