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1ckm

STRUCTURE OF TWO DIFFERENT CONFORMATIONS OF MRNA CAPPING ENZYME IN COMPLEX WITH GTP

Method: X-RAY DIFFRACTION D_max: 86.2 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1ckm
deposition_date	1997-04-20
title	STRUCTURE OF TWO DIFFERENT CONFORMATIONS OF MRNA CAPPING ENZYME IN COMPLEX WITH GTP
keywords	MRNA, CAPPING ENZYME, NUCLEOTIDYLTRANSFERASE; CAPPING ENZYME
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	27.62 Å
rg_electron	26.99 Å
i0	83166800.00
molecular_weight	73713.0 kDa
excluded_volume	93297 Å³
envelope_volume	113940 Å³
shell_volume	34846 Å³
envelope_diameter	87.6 Å
shell_rg	34.77 Å
envelope_rg	26.77 Å
shape_rg	26.98 Å
total_rg	27.83 Å
total_atoms	5186
n_residues	634
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	86.2 Å
rg_real	27.55 Å
rg_real_error	0.47 Å
i0_real	8.3170e+07
i0_real_error	1.1350e+06
rg_reciprocal	27.58 Å
i0_reciprocal	83170000.0000
total_estimate	0.9029
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	31.8 Å
skewness	0.288
kurtosis	-0.452
angular_range	— – 0.2850 Å⁻¹
current_alpha	0.0000
highest_alpha	27980000.0000
n_real_points	58
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.935; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.928

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	MRNA CAPPING ENZYME
formula_weight	37885.0 kDa
ec_number	2.7.7.50
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	5546

MVPPTINTGKNITTERAVLTLNGLQIKLHKVVGESRDDIVAKMKDLAMDDHKFPRLPGPNPVSIERKDFEKLKQNKYVVSEKTDGIRFMMFFTRVFGFKVCTIIDRAMTVYLLPFKNIPRVLFQGSIFDGELCVDIVEKKFAFVLFDAVVVSGVTVSQMDLASRFFAMKRSLKEFKNVPEDPAILRYKEWIPLEHPTIIKDHLKKANAIYHTDGLIIMSVDEPVIYGRNFNLFKLKPGTHHTIDFIIMSEDGTIGIFDPNLRKNVPVGKLDGYYNKGSIVECGFADGTWKYIQGRSDKNQANDRLTYEKTLLNIEENITIDELLDLFKWE

Source

organism	Paramecium bursaria Chlorella virus 1
ncbi_taxonomy_id	10506
host_organism	Escherichia coli

Entity 2 non-polymer

description	GUANOSINE-5'-TRIPHOSPHATE
formula_weight	523.2 kDa
src_method	syn
entity_count	2

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	360

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6. Citations (2)

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length_a	93.332 Å
length_b	214.931 Å
length_c	105.752 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	C 2 2 21
int_tables_number	20
crystal_system	—

resolution	2.500 Å
resolution_low	10.000 Å
r_factor	0.2180
r_work	0.2180
r_free	0.2990

fit	/app/data/saxs_computation/ck/1ckm/1ckm.fit
pddf	/app/data/pr_computation/ck/1ckm/1ckm.pr.out
profile	/app/data/saxs_computation/ck/1ckm/1ckm.dat

STRUCTURE OF TWO DIFFERENT CONFORMATIONS OF MRNA CAPPING ENZYME IN COMPLEX WITH GTP

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction