1clv

YELLOW MEAL WORM ALPHA-AMYLASE IN COMPLEX WITH THE AMARANTH ALPHA-AMYLASE INHIBITOR

Method: X-RAY DIFFRACTION Dmax: 77.2 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1clv

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1clv
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1clv
沉积日期 deposition_date1999-05-04
结构标题 titleYELLOW MEAL WORM ALPHA-AMYLASE IN COMPLEX WITH THE AMARANTH ALPHA-AMYLASE INHIBITOR
关键词 keywordsINSECT ALPHA-AMYLASE INHIBITOR, AMARANTHUS HYPOCHONDRIACUS, YELLOW MEAL WORM, KNOTTIN, HYDROLASE; HYDROLASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier23.74
回转半径 Rg (电子) rg_electron22.46
零角强度 I(0) i055650700.00
分子量 molecular_weight54863.0 kDa
排除体积 excluded_volume67008 ų
包络体积 envelope_volume75820 ų
水化壳体积 shell_volume27797 ų
包络直径 envelope_diameter80.3
壳层 Rg shell_rg30.06
包络 Rg envelope_rg22.62
形状 Rg shape_rg22.44
总 Rg total_rg23.29
总原子数 total_atoms3854
残基数 n_residues502
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax77.2
Rg (实空间) rg_real23.67
Rg 误差 (实空间) rg_real_error0.49
I(0) (实空间) i0_real5.5650e+07
I(0) 误差 (实空间) i0_real_error7.8250e+05
Rg (倒空间) rg_reciprocal23.69
I(0) (倒空间) i0_reciprocal55650000.0000
解质量估计 total_estimate0.8084
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary28.9
偏度 Skewness skewness0.315
峰度 Kurtosis kurtosis-0.245
角度范围 angular_range— – 0.3350 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha12610000.0000
实空间数据点数 n_real_points65
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.836; Stabil: 0.998; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.000

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (5)

6. 折叠分类 (SCOP + CATH) 5 domains

SCOP 2.08 (3 domains)

结构域编号 domain_idd1clva1
类 Class classb — All beta proteins
折叠类型 Fold foldb.71 — Glycosyl hydrolase domain
超家族 Superfamily superfamilyb.71.1 — Glycosyl hydrolase domain
家族 Family familyb.71.1.1 — alpha-Amylases, C-terminal beta-sheet domain
结构域编号 domain_idd1clva2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.8 — (Trans)glycosidases
家族 Family familyc.1.8.1 — Amylase, catalytic domain
结构域编号 domain_idd1clvi_
类 Class classg — Small proteins
折叠类型 Fold foldg.3 — Knottins (small inhibitors, toxins, lectins)
超家族 Superfamily superfamilyg.3.2 — Plant inhibitors of proteinases and amylases
家族 Family familyg.3.2.1 — Plant inhibitors of proteinases and amylases

CATH v4.4 (2 domains)

结构域编号 domain_id1clvA01
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily80 — Glycosidases
结构域编号 domain_id1clvA02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily1180 — Golgi alpha-mannosidase II

7. 引用文献 (1)

8. 文件与曲线 (10)