;SITE-DIRECTED MUTAGENESIS ON (SERINE) CARBOXYPEPTIDASE Y FROM YEAST. THE SIGNIFICANCE OF THR 60 AND MET 398 IN HYDROLYSIS AND AMINOLYSIS REACTIONS ;

Method: X-RAY DIFFRACTION D_max: 66.1 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cpy
deposition_date	1995-03-24
title	;SITE-DIRECTED MUTAGENESIS ON (SERINE) CARBOXYPEPTIDASE Y FROM YEAST. THE SIGNIFICANCE OF THR 60 AND MET 398 IN HYDROLYSIS AND AMINOLYSIS REACTIONS ;
keywords	HYDROLASE (CARBOXYPEPTIDASE); HYDROLASE (CARBOXYPEPTIDASE)
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	21.87 Å
rg_electron	20.49 Å
i0	36876800.00
molecular_weight	46468.0 kDa
excluded_volume	57669 Å³
envelope_volume	65925 Å³
shell_volume	25815 Å³
envelope_diameter	70.4 Å
shell_rg	28.05 Å
envelope_rg	20.72 Å
shape_rg	20.48 Å
total_rg	21.41 Å
total_atoms	3287
n_residues	421
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	66.1 Å
rg_real	21.67 Å
rg_real_error	0.29 Å
i0_real	3.6880e+07
i0_real_error	4.0810e+05
rg_reciprocal	21.71 Å
i0_reciprocal	36880000.0000
total_estimate	0.9001
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	29.6 Å
skewness	0.071
kurtosis	-0.470
angular_range	— – 0.3650 Å⁻¹
current_alpha	0.0000
highest_alpha	7265000.0000
n_real_points	68
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.917; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.980; Smooth: 0.966

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

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6. Citations (2)

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length_a	112.000 Å
length_b	112.000 Å
length_c	112.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	12

space_group	P 21 3
int_tables_number	198
crystal_system	—

resolution	2.600 Å
resolution_low	— Å
r_factor	0.1800
r_work	—
r_free	—

description	SERINE CARBOXYPEPTIDASE
formula_weight	47239.3 kDa
ec_number	3.4.16.5
mutation	E65A, E145A
src_method	nat
entity_count	1

type	polypeptide(L)
strand_id	A
atom_count	3325

;SITE-DIRECTED MUTAGENESIS ON (SERINE) CARBOXYPEPTIDASE Y FROM YEAST. THE SIGNIFICANCE OF THR 60 AND MET 398 IN HYDROLYSIS AND AMINOLYSIS REACTIONS ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Entity 2 non-polymer

Entity 3 water

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)

description	2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	221.2 kDa
src_method	man
entity_count	3

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	38

fit	/app/data/saxs_computation/cp/1cpy/1cpy.fit
pddf	/app/data/pr_computation/cp/1cpy/1cpy.pr.out
profile	/app/data/saxs_computation/cp/1cpy/1cpy.dat