1cxl
COMPLEX BETWEEN A COVALENT INTERMEDIATE AND BACILLUS CIRCULANS STRAIN 251 CGTASE E257Q
1. 蛋白身份与相关结构 Protein Identity & Related Structures
当前条目没有可用的 UniProt 蛋白身份。
七张关系表仍保留该条目的 assembly 与组成信息，但缺少统一蛋白身份时，不能可靠建立跨 PDB 的同蛋白链接。
当前条目的 assembly 组成
| Assembly | 聚集状态 | 实体与构建体证据 | 突变与修饰 | 配体、离子与共同组分 | 实验方法与环境 | 结构质量 |
|---|---|---|---|---|---|---|
| 1 | 其他组合 单体 蛋白 1 / DNA 0 / RNA 0 / 其他聚合物 4 PDB 声明：monomeric | 实体 1：PROTEIN (CYCLODEXTRIN-GLYCOSYLTRANSFERASE) × 1 实体 2：4-deoxy-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose × 1 实体 3：4-deoxy-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose × 1 实体 4：4-deoxy-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranosyl fluoride × 2 缺少 UniProt 身份时不显示参考序列区间 | 实体 1突变：E257Q | CA CALCIUM ION × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 | X-RAY DIFFRACTION X-ray结晶条件：pH 6.1；CRYSTAL SOAKED FIRST IN 4-DEOXY MALTOSE,THEN IN 4-DEOXY MALTOTRIOSYL ALPHA- FLUORIDE, pH 6.10 | 分辨率 1.81 Å R-free 0.190 |
页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。 链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。
SAXS 散射曲线 SAXS Profile
P(r) 距离分布 P(r) Distribution
2. 结构基本信息 2. Structure Basics
| 条目编号 entry_id | 1cxl |
| 沉积日期 deposition_date | 1999-02-27 |
| 结构标题 title | COMPLEX BETWEEN A COVALENT INTERMEDIATE AND BACILLUS CIRCULANS STRAIN 251 CGTASE E257Q |
| 关键词 keywords | GLYCOSYL HYDROLASE FAMILY 13, ALPHA-AMYLASE FAMILY, INTERMEDIATE COMPLEX, 4- DEOXYMALTOTRIOSE, GLYCOSYLTRANSFERASE; GLYCOSYLTRANSFERASE |
| 实验方法 method | X-RAY DIFFRACTION |
3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)
| 回转半径 Rg (Guinier) rg_guinier | 26.46 Å |
| 回转半径 Rg (电子) rg_electron | 25.29 Å |
| 零角强度 I(0) i0 | 97127300.00 |
| 分子量 molecular_weight | 76474.0 kDa |
| 排除体积 excluded_volume | 95017 ų |
| 包络体积 envelope_volume | 106930 ų |
| 水化壳体积 shell_volume | 34293 ų |
| 包络直径 envelope_diameter | 82.9 Å |
| 壳层 Rg shell_rg | 33.64 Å |
| 包络 Rg envelope_rg | 25.42 Å |
| 形状 Rg shape_rg | 25.28 Å |
| 总 Rg total_rg | 26.14 Å |
| 总原子数 total_atoms | 5394 |
| 残基数 n_residues | 686 |
| 球谐函数阶数 n_harmonics | 20 |
| q 范围 q_range | — – 0.5000 Å−1 |
| 数据点数 n_points | 101 |
| 壳层类型 shell_type | directional |
| 溶剂电子密度 solvent_density | 0.3340 e/ų |
| 壳层衬度 contrast_shell | 0.0300 e/ų |
| CRYSOL 版本 crysol_version | 4.1.3 |
4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)
| 最大尺寸 Dmax dmax | 80.0 Å |
| Rg (实空间) rg_real | 26.31 Å |
| Rg 误差 (实空间) rg_real_error | 0.48 Å |
| I(0) (实空间) i0_real | 9.7130e+07 |
| I(0) 误差 (实空间) i0_real_error | 1.2420e+06 |
| Rg (倒空间) rg_reciprocal | 26.36 Å |
| I(0) (倒空间) i0_reciprocal | 97130000.0000 |
| 解质量估计 total_estimate | 0.9118 |
| 解质量评级 solution_quality | EXCELLENT a EXCELLENT solution |
| P(r) 峰数 n_peaks | 2 |
| 主峰位置 r_peak_primary | 32.0 Å |
| 偏度 Skewness skewness | 0.151 |
| 峰度 Kurtosis kurtosis | -0.551 |
| 角度范围 angular_range | — – 0.3000 Å−1 |
| 当前正则化参数 α current_alpha | 0.0000 |
| 最高正则化参数 α highest_alpha | 19070000.0000 |
| 实空间数据点数 n_real_points | 61 |
| GNOM 版本 gnom_version | 4.1.3 |
| 质量判据 quality_criteria | AN1: 0.000; Oscil: 0.958; Stabil: 0.998; Sysdev: 1.000; Positv: 1.000; Valcen: 0.992; Smooth: 0.989 |
5. 晶体学与实验 5. Crystallography & Experiment
6. 实体与聚合物信息 Entities & Polymers (7)
7. 折叠分类 (SCOP + CATH) 8 domains
SCOP 2.08 (4 domains)
| 结构域编号 domain_id | d1cxla1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.1 — Immunoglobulin-like beta-sandwich |
| 超家族 Superfamily superfamily | b.1.18 — E set domains |
| 家族 Family family | b.1.18.2 — E-set domains of sugar-utilizing enzymes |
| 结构域编号 domain_id | d1cxla2 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.3 — Prealbumin-like |
| 超家族 Superfamily superfamily | b.3.1 — Starch-binding domain-like |
| 家族 Family family | b.3.1.1 — Starch-binding domain |
| 结构域编号 domain_id | d1cxla3 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.71 — Glycosyl hydrolase domain |
| 超家族 Superfamily superfamily | b.71.1 — Glycosyl hydrolase domain |
| 家族 Family family | b.71.1.1 — alpha-Amylases, C-terminal beta-sheet domain |
| 结构域编号 domain_id | d1cxla4 |
| 类 Class class | c — Alpha and beta proteins (a/b) |
| 折叠类型 Fold fold | c.1 — TIM beta/alpha-barrel |
| 超家族 Superfamily superfamily | c.1.8 — (Trans)glycosidases |
| 家族 Family family | c.1.8.1 — Amylase, catalytic domain |
CATH v4.4 (4 domains)
| 结构域编号 domain_id | 1cxlA01 |
| 类 Class class | 3 — Alpha Beta |
| 架构 Architecture architecture | 20 — Alpha-Beta Barrel |
| 拓扑 Topology topology | 20 — TIM Barrel |
| 同源超家族 H-superfamily homologous superfamily | 80 — Glycosidases |
| 结构域编号 domain_id | 1cxlA02 |
| 类 Class class | 2 — Mainly Beta |
| 架构 Architecture architecture | 60 — Sandwich |
| 拓扑 Topology topology | 40 — Immunoglobulin-like |
| 同源超家族 H-superfamily homologous superfamily | 1180 — Golgi alpha-mannosidase II |
| 结构域编号 domain_id | 1cxlA03 |
| 类 Class class | 2 — Mainly Beta |
| 架构 Architecture architecture | 60 — Sandwich |
| 拓扑 Topology topology | 40 — Immunoglobulin-like |
| 同源超家族 H-superfamily homologous superfamily | 10 — Immunoglobulins |
| 结构域编号 domain_id | 1cxlA04 |
| 类 Class class | 2 — Mainly Beta |
| 架构 Architecture architecture | 60 — Sandwich |
| 拓扑 Topology topology | 40 — Immunoglobulin-like |
| 同源超家族 H-superfamily homologous superfamily | 10 — Immunoglobulins |