BSOBI RESTRICTION ENDONUCLEASE
Geobacillus stearothermophilus
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein–DNA Homooligomer Protein × 2 DNA 2 PDB declaration: tetrameric（4） Consistent with all polymer counts | Chain A； UniProt 1–323 Chain B； UniProt 1–323 | Not recorded | ;DNA (5'-D(*T*AP*TP*AP*CP*TP*CP*GP*AP*GP*TP*AP*T)-3') ; × 2 DIO 1,4-DIETHYLENE DIOXIDE × 10 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7.6；293 K；EQUILIBRATION AGAINST RESERVOIR OF 20% (V/V) DIOXANE, 2% (V/V) ETHYLENE GLYCOL AND 5 MM DTT. DROPS WERE FORMED BY MIXING 2.5 UL PROTEIN-DNA COMPLEX (10 MG/ML PROTEIN; 1.1 FOLD MOLAR EXCESS DNA) IN BUFFER (20 MM TRIS-HCL PH 7.6, 300 MM NACL, 0.1 MM EDTA, 1 MM DTT, 0.02% NA AZIDE) WITH 2.5 UL DISTILLED WATER, 2.5 UL RESERVOIR SOLUTION AND 1.5 UL 350 MM N-HEPTYL-B-D-GLUCOSIDE, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K | Resolution 1.70 Å R-free 0.253 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
No other PDB entry for the same UniProt protein was found.
View Construct and Data Evidence
| UniProt name | T2B1_BACST |
| Isoform | — |
| PDB entities | 2 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–323； UniProt 1–323 Author chain B； PDBConstruct 1–323； UniProt 1–323 |
