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1dcn

INACTIVE MUTANT H162N OF DELTA 2 CRYSTALLIN WITH BOUND ARGININOSUCCINATE

Method: X-RAY DIFFRACTION D_max: 119.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1dcn
deposition_date	1998-10-29
title	INACTIVE MUTANT H162N OF DELTA 2 CRYSTALLIN WITH BOUND ARGININOSUCCINATE
keywords	EYE LENS PROTEIN, DELTA 2 CRYSTALLIN, ARGININOSUCCINATE LYASE; EYE LENS PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	36.92 Å
rg_electron	36.16 Å
i0	511197000.00
molecular_weight	189070.0 kDa
excluded_volume	239280 Å³
envelope_volume	295850 Å³
shell_volume	65156 Å³
envelope_diameter	129.7 Å
shell_rg	44.52 Å
envelope_rg	36.44 Å
shape_rg	36.16 Å
total_rg	36.68 Å
total_atoms	13283
n_residues	1712
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	119.1 Å
rg_real	36.74 Å
rg_real_error	0.74 Å
i0_real	5.1120e+08
i0_real_error	7.7770e+06
rg_reciprocal	36.86 Å
i0_reciprocal	511300000.0000
total_estimate	0.8932
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	44.3 Å
skewness	0.191
kurtosis	-0.458
angular_range	— – 0.2150 Å⁻¹
current_alpha	0.0000
highest_alpha	145500000.0000
n_real_points	44
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.888; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.945

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	DELTA 2 CRYSTALLIN
formula_weight	49500.8 kDa
ec_number	4.3.2.1
mutation	H162N
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	A,B,C,D
atom_count	13754

TDPIMEKLNSSIAYDQRLSEVDIQGSMAYAKALEKAGILTKTELEKILSGLEKISEEWSKGVFVVKQSDEDIHTANERRLKELIGDIAGKLNTGRSRNDQVVTDLKLFMKNSLSIISTHLLQLIKTLVERAAIEIDVILPGYTNLQKAQPIRWSQFLLSHAVALTRDSERLGEVKKRINVLPLGSGALAGNPLDIDREMLRSELEFASISLNSMDAISERDFVVEFLSFATLLMIHLSKMAEDLIIYSTSEFGFLTLSDAFSTGSSLMPQKKNPDSLELIRSKSGRVFGRLASILMVLKGLPSTYNKDLQEDKEAVFDVVDTLTAVLQVATGVISTLQISKENMEKALTPEMLATDLALYLVRKGVPFRQAHTASGKAVHLAETKGITINNLSLEDLKSISPQFSSDVSQVFNFVNSVEQYTALAGTAKSSVTTQIEQLRELMKKQK

Source

organism	Anas platyrhynchos
ncbi_taxonomy_id	8839
host_organism	Escherichia coli

Entity 2 non-polymer

description	ARGININOSUCCINATE
formula_weight	290.3 kDa
src_method	syn
entity_count	1

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	471

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6. Citations (1)

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length_a	94.580 Å
length_b	99.590 Å
length_c	107.140 Å
angle_alpha	90.00 °
angle_beta	101.68 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	2.300 Å
resolution_low	20.000 Å
r_factor	0.2290
r_work	0.2290
r_free	0.2900

fit	/app/data/saxs_computation/dc/1dcn/1dcn.fit
pddf	/app/data/pr_computation/dc/1dcn/1dcn.pr.out
profile	/app/data/saxs_computation/dc/1dcn/1dcn.dat

INACTIVE MUTANT H162N OF DELTA 2 CRYSTALLIN WITH BOUND ARGININOSUCCINATE

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction