1eak

Catalytic domain of proMMP-2 E404Q mutant

Method: X-RAY DIFFRACTION

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

72 KDA TYPE IV COLLAGENASE

HOMO SAPIENS

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	蛋白异源复合物	异源复合物	蛋白 3	INHIBITOR PEPTIDE × 1 SULFATE ION × 3 ZINC ION × 4 CALCIUM ION × 4 water × 2	与蛋白数一致
2	蛋白异源复合物	异源复合物	蛋白 3	INHIBITOR PEPTIDE × 1 SULFATE ION × 3 ZINC ION × 4 CALCIUM ION × 4 water × 2	与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	MM02_HUMAN
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–421； UniProt 32–452 作者链 B； PDB构建体 1–421； UniProt 32–452 作者链 C； PDB构建体 1–421； UniProt 32–452 作者链 D； PDB构建体 1–421； UniProt 32–452

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	1eak
沉积日期 deposition_date	2001-07-12
结构标题 title	Catalytic domain of proMMP-2 E404Q mutant
关键词 keywords	HYDROLASE-HYDROLASE INHIBITOR COMPLEX, HYDROLYSE, MATRIX METALLOPROTEINASE, GELATINASE A, HYDROLASE- HYDROLASE INHIBITOR COMPLEX; HYDROLASE/HYDROLASE INHIBITOR
实验方法 method	X-RAY DIFFRACTION

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3. Official assembly/model 理论 SAXS Official SAXS Profiles

页面只读取最新 assembly 计算结果。每一条曲线都对应明确的 PDB、official biological assembly 和 coordinate model。

1eak__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

下载该曲线 .dat

1eak__assembly_1__model_1 | I(q)

100 个绘图点；横轴和纵轴均采用对数尺度。

1eak__assembly_1__model_1 | P(r) · Pending

P(r) 将按相同 assembly/model 分别计算和展示。

Rg（Guinier）	32.26 Å
Rg（电子密度）	31.99 Å
总 Rg	32.61 Å
原子数	6702
残基数	847
排除体积	117480 Å³
最大 q	0.500 Å⁻¹

Assembly	Model	结构单元	聚集说明	状态	CRYSOL	操作
1	1	`1eak__assembly_1__model_1`	trimeric （3）	成功	4.1.3-1-20251215 (887e7ef)	查看下载
2	1	`1eak__assembly_2__model_1`	trimeric （3）	成功	4.1.3-1-20251215 (887e7ef)	查看下载

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合物信息 Entities & Polymers (6)

实体 1 Entity 1 polymer

描述 description	72 KDA TYPE IV COLLAGENASE
分子量 formula_weight	47304.3 kDa
EC 编号 ec_number	3.4.24.24
突变 mutation	YES
来源方法 src_method	man
拷贝数 entity_count	4

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D
原子数 atom_count	13504

SPIIKFPGDVAPKTDKELAVQYLNTFYGCPKESCNLFVLKDTLKKMQKFFGLPQTGDLDQNTIETMRKPRCGNPDVANYNFFPRKPKWDKNQITYRIIGYTPDLDPETVDDAFARAFQVWSDVTPLRFSRIHDGEADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFLWCSTTYNFEKDGKYGFCPHEALFTMGGNAEGQPCKFPFRFQGTSYDSCTTEGRTDGYRWCGTTEDYDRDKKYGFCPETAMSTVGGNSEGAPCVFPFTFLGNKYESCTSAGRSDGKMWCATTANYDDDRKWGFCPDQGYSLFLVAAHQFGHAMGLEHSQDPGALMAPIYTYTKNFRLSQDDIKGIQELYGASPDID

来源 Source

来源生物 organism	HOMO SAPIENS
通用名 common_name	HUMAN
NCBI 分类编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	TRICHOPLUSIA NI

实体 2 Entity 2 polymer

描述 description	INHIBITOR PEPTIDE
分子量 formula_weight	622.7 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	P,R

GPAGPPGA

实体 3 Entity 3 non-polymer

描述 description	SULFATE ION
分子量 formula_weight	96.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	6

实体 4 Entity 4 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	8

实体 5 Entity 5 non-polymer

描述 description	CALCIUM ION
分子量 formula_weight	40.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	8

实体 6 Entity 6 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	109

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6. 折叠分类 (SCOP + CATH) 40 domains

SCOP 2.08 (20 domains)

结构域编号 domain_id	d1eaka1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.20 — PGBD-like
超家族 Superfamily superfamily	a.20.1 — PGBD-like
家族 Family family	a.20.1.2 — MMP N-terminal domain

结构域编号 domain_id	d1eaka2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.92 — Zincin-like
超家族 Superfamily superfamily	d.92.1 — Metalloproteases ('zincins'), catalytic domain
家族 Family family	d.92.1.11 — Matrix metalloproteases, catalytic domain

结构域编号 domain_id	d1eaka3
类 Class class	g — Small proteins
折叠类型 Fold fold	g.14 — Kringle-like
超家族 Superfamily superfamily	g.14.1 — Kringle-like
家族 Family family	g.14.1.2 — Fibronectin type II module

结构域编号 domain_id	d1eaka4
类 Class class	g — Small proteins
折叠类型 Fold fold	g.14 — Kringle-like
超家族 Superfamily superfamily	g.14.1 — Kringle-like
家族 Family family	g.14.1.2 — Fibronectin type II module

结构域编号 domain_id	d1eaka5
类 Class class	g — Small proteins
折叠类型 Fold fold	g.14 — Kringle-like
超家族 Superfamily superfamily	g.14.1 — Kringle-like
家族 Family family	g.14.1.2 — Fibronectin type II module

结构域编号 domain_id	d1eakb1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.20 — PGBD-like
超家族 Superfamily superfamily	a.20.1 — PGBD-like
家族 Family family	a.20.1.2 — MMP N-terminal domain

结构域编号 domain_id	d1eakb2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.92 — Zincin-like
超家族 Superfamily superfamily	d.92.1 — Metalloproteases ('zincins'), catalytic domain
家族 Family family	d.92.1.11 — Matrix metalloproteases, catalytic domain

结构域编号 domain_id	d1eakb3
类 Class class	g — Small proteins
折叠类型 Fold fold	g.14 — Kringle-like
超家族 Superfamily superfamily	g.14.1 — Kringle-like
家族 Family family	g.14.1.2 — Fibronectin type II module

结构域编号 domain_id	d1eakb4
类 Class class	g — Small proteins
折叠类型 Fold fold	g.14 — Kringle-like
超家族 Superfamily superfamily	g.14.1 — Kringle-like
家族 Family family	g.14.1.2 — Fibronectin type II module

结构域编号 domain_id	d1eakb5
类 Class class	g — Small proteins
折叠类型 Fold fold	g.14 — Kringle-like
超家族 Superfamily superfamily	g.14.1 — Kringle-like
家族 Family family	g.14.1.2 — Fibronectin type II module

结构域编号 domain_id	d1eakc1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.20 — PGBD-like
超家族 Superfamily superfamily	a.20.1 — PGBD-like
家族 Family family	a.20.1.2 — MMP N-terminal domain

结构域编号 domain_id	d1eakc2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.92 — Zincin-like
超家族 Superfamily superfamily	d.92.1 — Metalloproteases ('zincins'), catalytic domain
家族 Family family	d.92.1.11 — Matrix metalloproteases, catalytic domain

结构域编号 domain_id	d1eakc3
类 Class class	g — Small proteins
折叠类型 Fold fold	g.14 — Kringle-like
超家族 Superfamily superfamily	g.14.1 — Kringle-like
家族 Family family	g.14.1.2 — Fibronectin type II module

结构域编号 domain_id	d1eakc4
类 Class class	g — Small proteins
折叠类型 Fold fold	g.14 — Kringle-like
超家族 Superfamily superfamily	g.14.1 — Kringle-like
家族 Family family	g.14.1.2 — Fibronectin type II module

结构域编号 domain_id	d1eakc5
类 Class class	g — Small proteins
折叠类型 Fold fold	g.14 — Kringle-like
超家族 Superfamily superfamily	g.14.1 — Kringle-like
家族 Family family	g.14.1.2 — Fibronectin type II module

结构域编号 domain_id	d1eakd1
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.20 — PGBD-like
超家族 Superfamily superfamily	a.20.1 — PGBD-like
家族 Family family	a.20.1.2 — MMP N-terminal domain

结构域编号 domain_id	d1eakd2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.92 — Zincin-like
超家族 Superfamily superfamily	d.92.1 — Metalloproteases ('zincins'), catalytic domain
家族 Family family	d.92.1.11 — Matrix metalloproteases, catalytic domain

结构域编号 domain_id	d1eakd3
类 Class class	g — Small proteins
折叠类型 Fold fold	g.14 — Kringle-like
超家族 Superfamily superfamily	g.14.1 — Kringle-like
家族 Family family	g.14.1.2 — Fibronectin type II module

结构域编号 domain_id	d1eakd4
类 Class class	g — Small proteins
折叠类型 Fold fold	g.14 — Kringle-like
超家族 Superfamily superfamily	g.14.1 — Kringle-like
家族 Family family	g.14.1.2 — Fibronectin type II module

结构域编号 domain_id	d1eakd5
类 Class class	g — Small proteins
折叠类型 Fold fold	g.14 — Kringle-like
超家族 Superfamily superfamily	g.14.1 — Kringle-like
家族 Family family	g.14.1.2 — Fibronectin type II module

CATH v4.4 (20 domains)

结构域编号 domain_id	1eakA01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	101 — Muramoyl-pentapeptide Carboxypeptidase; domain 1
同源超家族 H-superfamily homologous superfamily	10 — PGBD-like superfamily/PGBD

结构域编号 domain_id	1eakA02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	390 — Collagenase (Catalytic Domain)
同源超家族 H-superfamily homologous superfamily	10 — Collagenase (Catalytic Domain)

结构域编号 domain_id	1eakA03
类 Class class	2 — Mainly Beta
架构 Architecture architecture	10 — Ribbon
拓扑 Topology topology	10 — Seminal Fluid Protein PDC-109 (Domain B)
同源超家族 H-superfamily homologous superfamily	10 — Fibronectin, type II, collagen-binding

结构域编号 domain_id	1eakA04
类 Class class	2 — Mainly Beta
架构 Architecture architecture	10 — Ribbon
拓扑 Topology topology	10 — Seminal Fluid Protein PDC-109 (Domain B)
同源超家族 H-superfamily homologous superfamily	10 — Fibronectin, type II, collagen-binding

结构域编号 domain_id	1eakA05
类 Class class	2 — Mainly Beta
架构 Architecture architecture	10 — Ribbon
拓扑 Topology topology	10 — Seminal Fluid Protein PDC-109 (Domain B)
同源超家族 H-superfamily homologous superfamily	10 — Fibronectin, type II, collagen-binding

结构域编号 domain_id	1eakB01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	101 — Muramoyl-pentapeptide Carboxypeptidase; domain 1
同源超家族 H-superfamily homologous superfamily	10 — PGBD-like superfamily/PGBD

结构域编号 domain_id	1eakB02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	390 — Collagenase (Catalytic Domain)
同源超家族 H-superfamily homologous superfamily	10 — Collagenase (Catalytic Domain)

结构域编号 domain_id	1eakB03
类 Class class	2 — Mainly Beta
架构 Architecture architecture	10 — Ribbon
拓扑 Topology topology	10 — Seminal Fluid Protein PDC-109 (Domain B)
同源超家族 H-superfamily homologous superfamily	10 — Fibronectin, type II, collagen-binding

结构域编号 domain_id	1eakB04
类 Class class	2 — Mainly Beta
架构 Architecture architecture	10 — Ribbon
拓扑 Topology topology	10 — Seminal Fluid Protein PDC-109 (Domain B)
同源超家族 H-superfamily homologous superfamily	10 — Fibronectin, type II, collagen-binding

结构域编号 domain_id	1eakB05
类 Class class	2 — Mainly Beta
架构 Architecture architecture	10 — Ribbon
拓扑 Topology topology	10 — Seminal Fluid Protein PDC-109 (Domain B)
同源超家族 H-superfamily homologous superfamily	10 — Fibronectin, type II, collagen-binding

结构域编号 domain_id	1eakC01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	101 — Muramoyl-pentapeptide Carboxypeptidase; domain 1
同源超家族 H-superfamily homologous superfamily	10 — PGBD-like superfamily/PGBD

结构域编号 domain_id	1eakC02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	390 — Collagenase (Catalytic Domain)
同源超家族 H-superfamily homologous superfamily	10 — Collagenase (Catalytic Domain)

结构域编号 domain_id	1eakC03
类 Class class	2 — Mainly Beta
架构 Architecture architecture	10 — Ribbon
拓扑 Topology topology	10 — Seminal Fluid Protein PDC-109 (Domain B)
同源超家族 H-superfamily homologous superfamily	10 — Fibronectin, type II, collagen-binding

结构域编号 domain_id	1eakC04
类 Class class	2 — Mainly Beta
架构 Architecture architecture	10 — Ribbon
拓扑 Topology topology	10 — Seminal Fluid Protein PDC-109 (Domain B)
同源超家族 H-superfamily homologous superfamily	10 — Fibronectin, type II, collagen-binding

结构域编号 domain_id	1eakC05
类 Class class	2 — Mainly Beta
架构 Architecture architecture	10 — Ribbon
拓扑 Topology topology	10 — Seminal Fluid Protein PDC-109 (Domain B)
同源超家族 H-superfamily homologous superfamily	10 — Fibronectin, type II, collagen-binding

结构域编号 domain_id	1eakD01
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	101 — Muramoyl-pentapeptide Carboxypeptidase; domain 1
同源超家族 H-superfamily homologous superfamily	10 — PGBD-like superfamily/PGBD

结构域编号 domain_id	1eakD02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	390 — Collagenase (Catalytic Domain)
同源超家族 H-superfamily homologous superfamily	10 — Collagenase (Catalytic Domain)

结构域编号 domain_id	1eakD03
类 Class class	2 — Mainly Beta
架构 Architecture architecture	10 — Ribbon
拓扑 Topology topology	10 — Seminal Fluid Protein PDC-109 (Domain B)
同源超家族 H-superfamily homologous superfamily	10 — Fibronectin, type II, collagen-binding

结构域编号 domain_id	1eakD04
类 Class class	2 — Mainly Beta
架构 Architecture architecture	10 — Ribbon
拓扑 Topology topology	10 — Seminal Fluid Protein PDC-109 (Domain B)
同源超家族 H-superfamily homologous superfamily	10 — Fibronectin, type II, collagen-binding

结构域编号 domain_id	1eakD05
类 Class class	2 — Mainly Beta
架构 Architecture architecture	10 — Ribbon
拓扑 Topology topology	10 — Seminal Fluid Protein PDC-109 (Domain B)
同源超家族 H-superfamily homologous superfamily	10 — Fibronectin, type II, collagen-binding

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晶胞边长 a length_a	117.670 Å
晶胞边长 b length_b	166.150 Å
晶胞边长 c length_c	170.130 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	16

空间群 space_group	P 21 21 21
空间群编号 int_tables_number	19
晶系 crystal_system	—

分辨率 resolution	2.660 Å
最低分辨率 resolution_low	35.580 Å
R 因子 r_factor	0.2710
R_work r_work	0.2710
R_free r_free	0.3030

Catalytic domain of proMMP-2 E404Q mutant

1. 蛋白身份与相关结构 Protein Identity & Related Structures

72 KDA TYPE IV COLLAGENASE

当前结构中的状态

数据库中的同蛋白其他状态

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

1eak__assembly_1__model_1

1eak__assembly_1__model_1 | I(q)

1eak__assembly_1__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (6)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 polymer

聚合物 Polymer

实体 3 Entity 3 non-polymer

实体 4 Entity 4 non-polymer

实体 5 Entity 5 non-polymer

实体 6 Entity 6 water

6. 折叠分类 (SCOP + CATH) 40 domains

SCOP 2.08 (20 domains)

CATH v4.4 (20 domains)

7. 引用文献 (1)

Catalytic domain of proMMP-2 E404Q mutant

1. 蛋白身份与相关结构 Protein Identity & Related Structures

72 KDA TYPE IV COLLAGENASE

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

1eak__assembly_1__model_1

1eak__assembly_1__model_1 | I(q)

1eak__assembly_1__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (6)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 polymer

聚合物 Polymer

实体 3 Entity 3 non-polymer

实体 4 Entity 4 non-polymer

实体 5 Entity 5 non-polymer

实体 6 Entity 6 water

6. 折叠分类 (SCOP + CATH) 40 domains

SCOP 2.08 (20 domains)

CATH v4.4 (20 domains)

7. 引用文献 (1)