← Back to search

1ef0

CRYSTAL STRUCTURE OF PI-SCEI MINIPRECURSOR

Method: X-RAY DIFFRACTION D_max: 94.5 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1ef0
deposition_date	2000-02-04
title	CRYSTAL STRUCTURE OF PI-SCEI MINIPRECURSOR
keywords	endonuclease, protein splicing, mini-precursor, HYDROLASE; HYDROLASE
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	30.75 Å
rg_electron	29.64 Å
i0	134383000.00
molecular_weight	91628.0 kDa
excluded_volume	114730 Å³
envelope_volume	144940 Å³
shell_volume	39885 Å³
envelope_diameter	94.2 Å
shell_rg	37.72 Å
envelope_rg	29.42 Å
shape_rg	29.62 Å
total_rg	30.45 Å
total_atoms	6442
n_residues	819
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	94.5 Å
rg_real	30.57 Å
rg_real_error	0.45 Å
i0_real	1.3440e+08
i0_real_error	1.7240e+06
rg_reciprocal	30.65 Å
i0_reciprocal	134400000.0000
total_estimate	0.9076
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	1
r_peak_primary	40.0 Å
skewness	0.105
kurtosis	-0.575
angular_range	— – 0.2600 Å⁻¹
current_alpha	0.0000
highest_alpha	24650000.0000
n_real_points	53
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.951; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.994; Smooth: 0.947

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (3)

Entity 1 polymer

description	PI-SCEI ENDONUCLEASE
formula_weight	51803.8 kDa
ec_number	3.6.1.34
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	6887

KLEGAFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRETMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEVITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARDLSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSDRATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRGNGIRNNLNTENPLWDAIVGLGFLKDGVKNIPSFLSTDNIGTRETFLAGLIDSDGYVTDEHGIKATIKTIHTSVRDGLVSLARSLGLVVSVNAEPAKVDMNGTKHKISYAIYMSGGDVLLNVLSKCAGSKKFRPAPAAAFARECRGFYFELQELKEDDYYGITLSDDSDHQFLLANQVVVHACGER

Source

organism	Saccharomyces cerevisiae
common_name	;baker's yeast ;
ncbi_taxonomy_id	4932
host_organism	Escherichia coli

Entity 2 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	2

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	408

▶

6. Citations (4)

▶

length_a	59.003 Å
length_b	101.683 Å
length_c	86.765 Å
angle_alpha	90.00 °
angle_beta	93.51 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	2.100 Å
resolution_low	20.000 Å
r_factor	0.2310
r_work	0.2310
r_free	0.2810

fit	/app/data/saxs_computation/ef/1ef0/1ef0.fit
pddf	/app/data/pr_computation/ef/1ef0/1ef0.pr.out
profile	/app/data/saxs_computation/ef/1ef0/1ef0.dat

CRYSTAL STRUCTURE OF PI-SCEI MINIPRECURSOR

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction